CEP135 (NM_025009) Human Tagged ORF Clone

CAT#: RC224008

CEP135 (Myc-DDK-tagged)-Human centrosomal protein 135kDa (CEP135)

Product Images

Circular map for RC224008

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CEP135
• Synonyms: CEP4; KIAA0635; MCPH8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224008 representing NM_025009
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACAGCTGTAGAGAGAAAGTATATTAATATTAGGAAAAGGCTGGATCAGCTGGGATACCGCCAGA
  CTCTGACAGTGGAGTGTTTACCTTTGGTAGAAAAACTTTTCAGCGACTTAGTTCATACAACTGAGAGCCT
  TCGGCAATCAAAATTATCTGCTGTGAAAGCTGAAAAAGAAAGTGCCAATTTTGATTTTGTTTTGGAACCC
  TATAAACTTGAAAATGCAAGATTGAGTAGAGAAAATAATGAATTATACCTAGAGTTAATGAAACTGAGAG
  AACATTCAGACCAACACGTTAAAGAGTTGAAAACTTCATTGAAGAAATGTGCACGTGAAACAGCTGATCT
  GAAATTTCTGAATAACCAATATGCTCATAAACTCAAACTGTTGGAGAAAGAGAGCAAAGCTAAGAATGAA
  AGAATTCAACAACTTCAAGAAAAGAATTTGCATGCTGTAGTACAAACTCCAGGTGGCAAGAAAAGAAGTA
  TTGCTTTCAGGCGCCAGCGTATGCAAATTGATGAACCGGTTCCTCCCTCTGAAGTCAGTTCATATCCAGT
  TCCTCAACCAGATGACCCTTACATTGCAGACCTCCTTCAAGTGGCTGATAACAGGATTCAAGAACTTCAA
  CAGGAAGTCCACCAGCTACAAGAAAAGTTAGCAATGATGGAAAGTGGGGTGAGAGACTATAGCAAGCAGA
  TTGAGCTAAGAGAACGAGAGATAGAACGACTGTCAGTTGCTTTGGATGGTGGTCGGTCCCCTGATGTCCT
  TTCTCTGGAGTCTAGAAATAAAACCAATGAAAAGCTTATTGCTCATTTAAATATTCAGGTTGACTTTCTT
  CAGCAAGCTAATAAAGACCTGGAGAAGCGTATACGAGAGCTTATGGAAACCAAGGAAACAGTGACATCTG
  AAGTCGTTAATTTAAGTAACAAAAATGAAAAACTCTGCCAAGAATTAACTGAAATAGATCAGTTAGCACA
  GCAGTTGGAAAGACATAAAGAAGAAGTGCTTGAGACTGCTGATAAAGAGCTTGGGGAAGCAAAGAAAGAG
  ATTAAAAGAAAGCTCTCTGAAATGCAGGATCTTGAAGAAACAATGGCAAAACTTCAGCTGGAATTGAACT
  TATGCCAGAAAGAAAAGGAGAGACTGAGTGATGAACTCCTTGTAAAATCAGACCTAGAAACTGTTGTTCA
  TCAGCTTGAACAAGAAAAGCAAAGACTTAGCAAAAAAGTTGAAAGTTTTGCAGTTACAGAACGACAACTT
  ACTCTGGAGGTTGAGAGGATGAGACTAGAACATGGAATAAAACGTCGAGACAGGTCACCTTCTCGTTTAG
  ATACATTTCTGAAAGGTATAGAAGAAGAACGAGATTATTATAAGAAAGAGCTAGAGAGACTCCAACATAT
  AATACAGCGAAGATCTTGCTCTACAAGTTATAGCGCACGTGAAAAAAGTTCAATATTTAGAACACCAGAA
  AAGGGTGATTACAATTCAGAAATTCATCAGATCACAAGAGAAAGAGATGAACTTCAGCGTATGCTAGAAA
  GATTTGAAAAATATATGGAAGATATACAGTCCAATGTTAAATTATTGACAGCAGAAAGAGATAAACTAAG
  TGTCTTATATAATGAAGCTCAGGAAGAATTATCTGCCCTAAGAAAGGAATCCACCCAAACCACAGCACCC
  CATAATATTGTTAGTCTTATGGAAAAGGAAAAAGAACTTGCGTTATCTGACTTAAGAAGAATTATGGCAG
  AAAAGGAAGCTTTAAGAGAAAAATTAGAGCATATTGAAGAAGTGAGTCTTTTTGGAAAATCAGAATTAGA
  GAAAACTATTGAACATTTGACATGTGTTAATCATCAGCTTGAAAGCGAAAAATATGAATTAAAGTCTAAA
  GTGTTAATAATGAAAGAAACAATAGAGTCGTTAGAGAACAAATTAAAAGTCCAAGCTCAAAAATTTAGCC
  ATGTGGCTGGTGACTCATCTCATCAGAAAACAGAGGTGAACTCACTTAGGATAGTGAATGAGCAGCTACA
  GCGGTCAGTTGATGACTATCAGCACCGACTTTCCATAAAAAGAGGTGAACTTGAATCAGCCCAAGCACAA
  ATTAAAATACTGGAGGAAAAGATAGATGAACTAAACCTTAAGATGACTTCACAGGATGAGGAGGCTCATG
  TAATGAAAAAGACCATTGGTGTTATTGATAAAGAAAAAGACTTTCTCCAGGAGACTGTAGATGAGAAGAC
  AGAAAAGATTGCAAATTTGCAAGAAAACCTAGCTAATAAAGAAAAAGCTGTTGCTCAAATGAAGATAATG
  ATCTCAGAGTGTGAATCATCTGTGAACCAGCTGAAAGAAACATTGGTTAATCGAGATCGTGAGATAAACA
  GCCTCCGGCGCCAGCTTGATGCAGCTCACAAAGAACTCGATGAAGTAGGAAGATCTAGAGAAATCGCTTT
  TAAGGAAAACAGAAGACTGCAAGATGACCTGGCTACAATGGCAAGAGAAAATCAAGAAATCTCATTGGAA
  TTGGAAGCAGCAGTGCAAGAAAAAGAAGAAATGAAGAGCAGAGTTCATAAATACATAACAGAGGTGTCAC
  GATGGGAGAGCTTAATGGCTGCCAAGGAAAAAGAAAATCAAGATTTGTTAGATAGATTTCAGATGCTTCA
  TAACCGTGCTGAAGACTGGGAGGTCAAAGCCCATCAAGCTGAGGGAGAAAGCAGCTCAGTTCGACTGGAA
  CTTCTTTCTATTGACACTGAGAGGAGACATCTTCGAGAAAGAGTGGAGCTATTAGAAAAAGAAATTCAAG
  AGCACATAAATGCCCATCATGCTTATGAATCTCAGATCTCATCAATGGCAAAAGCCATGTCTCGATTAGA
  AGAAGAGCTGAGACATCAAGAAGATGAGAAAGCAACAGTATTAAATGACTTGTCATCTCTTAGAGAACTT
  TGCATTAAACTTGATTCAGGCAAAGATATTATGACCCAGCAATTGAATTCGAAAAACCTTGAGTTTGAGA
  GGGTTGTGGTGGAATTAGAAAATGTAAAGTCAGAGTCAGACCTACTGAAAAAACAACTTTCAAATGAGAG
  ACATACAGTTAAAAACCTCGAATCATTGTTGGCTACAAACAGAGATAAAGAATTTCATTCTCACTTAACC
  TCCCACGAGAAGGATACAGAAATCCAGCTACTTAAGGAGAAGTTAACCCTTTCTGAAAGCAAATTAACTA
  GTCAAAGCCGGGAAAACACCATGCTTCGAGCTAAAGTGGCACAGTTACAAACAGATTATGATGCTCTGAA
  AAGGCAGATCTCAACTGAAAGATACGAACGAGAACGAGCAATCCAAGAGATGCGTCGACATGGTCTTGCT
  ACACCACCCCTTAGTTCCACTCTGAGGTCTCCTTCACATTCTCCTGAACATAGAAATGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224008 representing NM_025009
  Red=Cloning site Green=Tags(s)
  
  MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEP
  YKLENARLSRENNELYLELMKLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNE
  RIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPSEVSSYPVPQPDDPYIADLLQVADNRIQELQ
  QEVHQLQEKLAMMESGVRDYSKQIELREREIERLSVALDGGRSPDVLSLESRNKTNEKLIAHLNIQVDFL
  QQANKDLEKRIRELMETKETVTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKKE
  IKRKLSEMQDLEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQL
  TLEVERMRLEHGIKRRDRSPSRLDTFLKGIEEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPE
  KGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLYNEAQEELSALRKESTQTTAP
  HNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSLFGKSELEKTIEHLTCVNHQLESEKYELKSK
  VLIMKETIESLENKLKVQAQKFSHVAGDSSHQKTEVNSLRIVNEQLQRSVDDYQHRLSIKRGELESAQAQ
  IKILEEKIDELNLKMTSQDEEAHVMKKTIGVIDKEKDFLQETVDEKTEKIANLQENLANKEKAVAQMKIM
  ISECESSVNQLKETLVNRDREINSLRRQLDAAHKELDEVGRSREIAFKENRRLQDDLATMARENQEISLE
  LEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDLLDRFQMLHNRAEDWEVKAHQAEGESSSVRLE
  LLSIDTERRHLRERVELLEKEIQEHINAHHAYESQISSMAKAMSRLEEELRHQEDEKATVLNDLSSLREL
  CIKLDSGKDIMTQQLNSKNLEFERVVVELENVKSESDLLKKQLSNERHTVKNLESLLATNRDKEFHSHLT
  SHEKDTEIQLLKEKLTLSESKLTSQSRENTMLRAKVAQLQTDYDALKRQISTERYERERAIQEMRRHGLA
  TPPLSSTLRSPSHSPEHRNV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025009
• ORF Size: 3420 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025009.5
• RefSeq Size: 5760 bp
• RefSeq ORF: 3423 bp
• Locus ID: 9662
• UniProt ID: Q66GS9
• Cytogenetics: 4q12
• MW: 133.5 kDa
• Gene Summary: This gene encodes a centrosomal protein, which acts as a scaffolding protein during early centriole biogenesis, and is also required for centriole-centriole cohesion during interphase. Mutations in this gene are associated with autosomal recessive primary microcephaly-8. [provided by RefSeq, Jun 2012]