CACHD1 (NM_020925) Human Tagged ORF Clone

CAT#: RC224006

CACHD1 (Myc-DDK-tagged)-Human cache domain containing 1 (CACHD1)

Product Images

Circular map for RC224006

• 

  Western blot validation of overexpression lysate (Cat# LY412223) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224006 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CACHD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224006 representing NM_020925
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGAGGCTGGCGGCCGAGGAGCTGGGGGTCGTCACCATGCAGCGGATATTCAACTCCTTTGTTTACA
  CTGAGAAAATCTCAAATGGAGAAAGTGAAGTACAGCAGCTAGCCAAAAAAATCCGAGAGAAGTTCAACCG
  TTACTTGGATGTGGTCAATCGGAACAAGCAAGTTGTAGAAGCATCCTATACGGCTCACCTAACCTCTCCC
  CTAACTGCAATTCAAGACTGCTGTACTATCCCACCTTCCATGATGGAATTCGATGGGAACTTTAATACCA
  ATGTGTCTAGAACAATTAGTTGTGATCGACTTTCTACTACTGTTAATAGCCGGGCCTTCAATCCAGGACG
  AGACTTAAATTCAGTTCTTGCAGACAACCTGAAATCCAACCCTGGAATTAAGTGGCAATATTTCAGTTCA
  GAAGAAGGAATTTTCACTGTTTTCCCAGCACACAAGTTCCGGTGTAAGGGCAGCTACGAACACCGCAGTA
  GACCCATCTACGTCTCTACAGTCCGGCCGCAGTCAAAGCACATAGTAGTGATTCTGGACCACGGGGCTTC
  AGTCACAGACACTCAGCTTCAGATTGCCAAGGACGCTGCTCAGGTCATCCTCAGCGCCATCGATGAACAT
  GACAAGATTTCTGTGTTAACTGTGGCAGATACCGTCCGGACTTGCTCACTAGACCAGTGCTATAAGACCT
  TCTTGTCTCCAGCCACCAGTGAGACAAAAAGGAAAATGTCCACCTTTGTTAGCAGCGTGAAGTCTTCAGA
  CAGTCCTACCCAGCACGCAGTGGGATTCCAAAAGGCATTTCAGCTGATTCGAAGTACAAACAATAACACA
  AAGTTCCAAGCAAATACAGACATGGTCATCATTTACCTGTCAGCTGGCATTACATCAAAGGACTCTTCGG
  AAGAAGATAAAAAAGCGACTCTCCAAGTCATCAATGAAGAAAATAGCTTTCTAAACAACTCTGTAATGAT
  TCTCACCTATGCCCTCATGAACGATGGGGTGACTGGTTTGAAAGAGCTGGCTTTTCTGAGGGATCTAGCT
  GAACAGAATTCAGGGAAGTACGGTGTGCCAGACCGGATGGCCTTGCCTGTGATTAAGGGCAGCATGATGG
  TGCTGAATCAGTTGAGCAACCTGGAGACCACAGTGGGCAGGTTCTACACAAACCTTCCCAACCGGATGAT
  TGATGAAGCCGTCTTCAGCCTGCCCTTCTCTGATGAGATGGGAGATGGTTTGATAATGACTGTGAGTAAA
  CCCTGTTATTTTGGAAACCTACTTCTGGGAATTGTAGGTGTGGACGTGAATCTGGCTTACATTCTTGAAG
  ACGTGACGTATTACCAAGACTCTTTGGCTTCCTATACTTTTCTCATAGACGACAAAGGATATACACTTAT
  GCACCCATCTCTTACCAGGCCATATTTATTGTCAGAGCCCCCACTTCATACTGACATCATACATTATGAA
  AATATTCCAAAATTTGAATTAGTTCGGCAAAATATCCTAAGCCTCCCTCTGGGCAGCCAGATTATCGCAG
  TCCCTGTGAACTCATCCCTGTCTTGGCACATAAACAAGCTGAGAGAAACTGGAAAGGAAGCCTACAATGT
  TAGCTATGCCTGGAAGATGGTACAAGACACTTCCTTTATTCTGTGTATTGTGGTGATACAACCAGAAATA
  CCTGTGAAACAACTGAAGAACCTCAACACTGTTCCCAGCAGCAAGCTGCTGTACCACCGGCTGGATCTCC
  TTGGCCAGCCCAGTGCTTGCCTCCACTTCAAACAGCTGGCAACCCTAGAAAGTCCCACCATCATGCTGTC
  TGCTGGCAGCTTTTCCTCCCCCTATGAGCACCTCAGCCAGCCAGAGACAAAGCGCATGGTAGAGCACTAC
  ACCGCCTATCTCAGCGACAACACCCGCCTCATTGCTAACCCGGGCCTCAAATTCTCTGTCAGAAATGAAG
  TAATGGCTACCAGCCACGTCACAGATGAATGGATGACACAAATGGAAATGAGTAGCCTGAACACTTACAT
  TGTCCGCCGTTACATAGCAACACCCAATGGCGTCCTCAGAATTTATCCTGGTTCCCTCATGGACAAAGCA
  TTTGATCCCACTAGGAGACAATGGTATCTCCATGCAGTAGCTAATCCAGGGTTGATTTCTTTGACTGGTC
  CTTACTTAGATGTTGGAGGAGCTGGTTATGTTGTGACAATCAGTCACACAATTCATTCATCCAGTACACA
  GCTGTCTTCTGGGCACACTGTGGCTGTGATGGGCATTGACTTCACACTCAGATACTTCTACAAAGTTCTG
  ATGGACCTATTACCTGTCTGTAACCAAGATGGTGGCAACAAAATAAGGTGCTTCATAATGGAGGACAGGG
  GTTATCTGGTGGCGCACCCGACTCTCATCGACCCCAAAGGACATGCACCTGTGGAGCAGCAGCACATCAC
  CCACAAGGAGCCCCTGGTAGCAAATGATATCCTCAACCACCCCAACTTTGTAAAGAAAAACCTGTGCAAC
  AGCTTCAGTGACAGAACGGTCCAGAGGTTTTATAAATTCAACACCAGCCTTGCGGGGGATTTGACGAACC
  TTGTGCATGGCAGCCACTGTTCCAAATACAGATTAGCAAGGATCCCAGGAACCAACGCGTTTGTTGGCAT
  TGTCAACGAAACCTGCGACTCTCTTGCCTTCTGTGCCTGCAGCATGGTGGACCGACTCTGTCTCAACTGT
  CACCGAATGGAACAAAATGAATGTGAATGTCCTTGTGAGTGCCCTCTAGAGGTCAATGAGTGCACTGGCA
  ACCTCACCAATGCAGAGAACCGAAACCCCAGCTGCGAGGTCCACCAGGAGCCGGTGACATACACAGCTAT
  TGACCCTGGCCTGCAAGATGCTCTTCACCAGTGTGTCAACAGCAGGTGCAGTCAGAGGCTGGAAAGTGGG
  GACTGTTTTGGGGTGCTGGATTGTGAATGGTGCATGGTGGACAGTGATGGAAAGACTCACCTGGACAAAC
  CCTACTGTGCCCCCCAGAAAGAATGCTTCGGGGGGATTGTGGGAGCCAAAAGTCCCTACGTTGATGACAT
  GGGAGCAATAGGTGATGAGGTGATCACATTAAACATGATTAAAAGCGCCCCTGTGGGTCCTGTGGCTGGA
  GGGATCATGGGATGCATCATGGTCTTGGTCCTGGCGGTGTATGCCTACCGCCACCAGATTCATCGCCGGA
  GCCATCAGCATATGTCTCCTCTTGCTGCCCAAGAAATGTCAGTGCGTATGTCCAACCTGGAGAATGACAG
  AGATGAAAGGGACGACGACAGCCACGAAGACAGAGGCATCATCAGCAACACTCGGTTTATAGCTGCGGTC
  ATCGAACGACATGCACACAGTCCAGAAAGAAGGCGCCGCTACTGGGGTCGATCAGGAACAGAAAGTGATC
  ATGGTTACAGCACCATGAGCCCACAGGAGGACAGTGAAAATCCTCCATGCAACAATGACCCCTTGTCAGC
  CGGGGTCGATGTGGGAAACCATGATGAGGACTTAGACCTGGATACCCCCCCTCAGACTGCTGCCCTACTA
  AGTCACAAGTTCCACCACTACCGGTCACACCACCCTACACTTCATCATAGCCACCACTTACAGGCGGCCG
  TCACGGTACACACTGTCGATGCAGAATGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224006 representing NM_020925
  Red=Cloning site Green=Tags(s)
  
  MRRLAAEELGVVTMQRIFNSFVYTEKISNGESEVQQLAKKIREKFNRYLDVVNRNKQVVEASYTAHLTSP
  LTAIQDCCTIPPSMMEFDGNFNTNVSRTISCDRLSTTVNSRAFNPGRDLNSVLADNLKSNPGIKWQYFSS
  EEGIFTVFPAHKFRCKGSYEHRSRPIYVSTVRPQSKHIVVILDHGASVTDTQLQIAKDAAQVILSAIDEH
  DKISVLTVADTVRTCSLDQCYKTFLSPATSETKRKMSTFVSSVKSSDSPTQHAVGFQKAFQLIRSTNNNT
  KFQANTDMVIIYLSAGITSKDSSEEDKKATLQVINEENSFLNNSVMILTYALMNDGVTGLKELAFLRDLA
  EQNSGKYGVPDRMALPVIKGSMMVLNQLSNLETTVGRFYTNLPNRMIDEAVFSLPFSDEMGDGLIMTVSK
  PCYFGNLLLGIVGVDVNLAYILEDVTYYQDSLASYTFLIDDKGYTLMHPSLTRPYLLSEPPLHTDIIHYE
  NIPKFELVRQNILSLPLGSQIIAVPVNSSLSWHINKLRETGKEAYNVSYAWKMVQDTSFILCIVVIQPEI
  PVKQLKNLNTVPSSKLLYHRLDLLGQPSACLHFKQLATLESPTIMLSAGSFSSPYEHLSQPETKRMVEHY
  TAYLSDNTRLIANPGLKFSVRNEVMATSHVTDEWMTQMEMSSLNTYIVRRYIATPNGVLRIYPGSLMDKA
  FDPTRRQWYLHAVANPGLISLTGPYLDVGGAGYVVTISHTIHSSSTQLSSGHTVAVMGIDFTLRYFYKVL
  MDLLPVCNQDGGNKIRCFIMEDRGYLVAHPTLIDPKGHAPVEQQHITHKEPLVANDILNHPNFVKKNLCN
  SFSDRTVQRFYKFNTSLAGDLTNLVHGSHCSKYRLARIPGTNAFVGIVNETCDSLAFCACSMVDRLCLNC
  HRMEQNECECPCECPLEVNECTGNLTNAENRNPSCEVHQEPVTYTAIDPGLQDALHQCVNSRCSQRLESG
  DCFGVLDCEWCMVDSDGKTHLDKPYCAPQKECFGGIVGAKSPYVDDMGAIGDEVITLNMIKSAPVGPVAG
  GIMGCIMVLVLAVYAYRHQIHRRSHQHMSPLAAQEMSVRMSNLENDRDERDDDSHEDRGIISNTRFIAAV
  IERHAHSPERRRRYWGRSGTESDHGYSTMSPQEDSENPPCNNDPLSAGVDVGNHDEDLDLDTPPQTAALL
  SHKFHHYRSHHPTLHHSHHLQAAVTVHTVDAEC
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020925
• ORF Size: 3669 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020925.2, NP_065976.2
• RefSeq Size: 5274 bp
• RefSeq ORF: 3825 bp
• Locus ID: 57685
• UniProt ID: Q5VU97
• Cytogenetics: 1p31.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 136.5 kDa
• Gene Summary: May regulate voltage-dependent calcium channels.[UniProtKB/Swiss-Prot Function]