Eco1 (ESCO1) (NM_052911) Human Tagged ORF Clone

CAT#: RC224000

ESCO1 (Myc-DDK-tagged)-Human establishment of cohesion 1 homolog 1 (S. cerevisiae) (ESCO1)

Product Images

Circular map for RC224000

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Eco1
• Synonyms: A930014I12Rik; CTF; ECO1; EFO1; ESO1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224000 representing NM_052911
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTCCATTCAGGAGAAATCAAAAGAGAATTCCTCCAAAGTTACTAAAAAAAGTGACGATAAGAATT
  CAGAAACAGAAATTCAGGATTCTCAAAAGAATCTAGCAAAAAAATCAGGTCCAAAGGAGACTATAAAATC
  ACAGGCTAAATCTTCCAGTGAAAGTAAAATAAATCAGCCAGAATTGGAAACACGCATGAGTACAAGGTCA
  TCAAAGGCAGCATCTAATGATAAAGCTACTAAATCCATTAATAAAAATACGGTGACTGTGAGGGGATATT
  CACAAGAATCTACAAAAAAGAAATTATCTCAGAAAAAATTAGTACATGAAAACCCTAAAGCAAATGAACA
  GCTTAACCGGAGATCACAAAGGCTACAACAATTAACAGAGGTTTCAAGAAGGTCGTTACGCAGTAGAGAA
  ATTCAGGGTCAAGTTCAAGCAGTTAAACAGAGTTTGCCACCAACTAAAAAAGAGCAGTGTAGCAGTACTC
  AGAGTAAATCTAATAAAACAAGTCAAAAACATGTGAAGAGAAAAGTACTGGAAGTAAAGTCTGACTCTAA
  AGAAGATGAAAATCTAGTAATTAATGAAGTAATAAATTCTCCCAAAGGGAAAAAACGCAAGGTAGAACAT
  CAGACAGCTTGTGCTTGTAGTTCTCAATGCACGCAAGGATCTGAAAAGTGTCCTCAGAAGACTACTAGAA
  GAGACGAAACGAAACCTGTGCCTGTAACTTCTGAGGTGAAAAGATCAAAAATGGCTACTTCAGTGGTCCC
  GAAAAAGAATGAGATGAAGAAGTCGGTTCATACACAAGTGAATACTAACACAACACTCCCAAAAAGTCCA
  CAGCCATCAGTGCCTGAACAAAGTGATAATGAGCTGGAGCAAGCAGGAAAGAGCAAACGAGGTAGTATTC
  TCCAGCTCTGTGAAGAAATTGCTGGTGAAATTGAGTCAGATAATGTAGAGGTAAAAAAGGAATCTTCACA
  AATGGAAAGTGTAAAGGAAGAAAAGCCCACAGAAATAAAATTGGAAGAGACCAGTGTTGAAAGACAAATA
  CTTCATCAGAAGGAAACAAATCAGGATGTGCAATGTAATCGTTTTTTCCCAAGTAGAAAAACAAAGCCTG
  TGAAATGTATACTAAATGGAATAAACAGCTCAGCCAAGAAGAACTCCAACTGGACTAAAATTAAACTCTC
  AAAATTTAACTCTGTGCAGCACAATAAGTTGGACTCTCAAGTTTCCCCTAAATTAGGCTTATTACGAACC
  AGTTTTTCACCACCAGCTTTAGAAATGCATCATCCAGTGACTCAAAGTACGTTTTTAGGGACAAAGCTAC
  ATGATAGAAATATAACTTGCCAGCAGGAAAAAATGAAAGAAATTAATTCTGAAGAAGTGAAAATTAATGA
  TATTACAGTAGAAATTAATAAAACCACAGAAAGGGCTCCTGAAAATTGTCATTTGGCCAATGAGATAAAA
  CCTTCTGACCCACCATTGGATAATCAGATGAAACATTCTTTTGATTCAGCATCAAATAAGAATTTCAGCC
  AATGTTTGGAATCCAAGCTAGAAAACAGTCCAGTGGAAAATGTTACTGCTGCTTCGACTCTGCTCAGTCA
  AGCAAAAATTGATACAGGAGAGAATAAATTTCCAGGTTCAGCTCCCCAACAGCATAGTATTCTCAGTAAC
  CAGACATCTAAAAGCAGTGATAACAGGGAGACACCACGAAATCATTCTTTGCCTAAGTGTAATTCCCATT
  TGGAGATAACAATTCCAAAGGACTTGAAACTAAAAGAAGCAGAGAAAACTGATGAAAAACAGTTGATTAT
  AGATGCAGGACAAAAAAGATTTGGAGCAGTTTCTTGTAATGTTTGTGGAATGCTGTATACAGCTTCAAAT
  CCAGAAGATGAAACACAGCATCTGCTTTTCCACAACCAGTTTATAAGTGCTGTTAAATATGTGGGCTGGA
  AGAAAGAAAGAATTCTGGCTGAATACCCTGATGGCAGGATAATAATGGTTCTTCCTGAAGACCCAAAGTA
  TGCCCTGAAAAAGGTTGACGAGATTAGAGAGATGGTTGACAATGATTTAGGTTTTCAACAGGCTCCACTA
  ATGTGCTATTCCAGAACTAAAACACTTCTCTTCATTTCCAATGACAAAAAAGTAGTTGGCTGCCTAATTG
  CGGAACATATCCAATGGGGCTACAGAGTTATAGAAGAGAAACTTCCAGTTATCAGGTCAGAAGAAGAAAA
  AGTCAGATTTGAAAGGCAAAAAGCCTGGTGCTGCTCAACATTACCAGAGCCTGCAATCTGCGGGATCAGT
  CGAATATGGGTATTCAGCATGATGCGTCGGAAGAAAATTGCTTCTCGCATGATTGAATGCCTAAGGAGTA
  ACTTTATATATGGCTCATATTTGAGCAAAGAAGAAATTGCTTTCTCAGATCCCACTCCTGATGGAAAGCT
  GTTTGCAACACAGTACTGTGGCACTGGTCAATTTCTGGTATATAATTTTATTAATGGACAGAATAGCACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224000 representing NM_052911
  Red=Cloning site Green=Tags(s)
  
  MMSIQEKSKENSSKVTKKSDDKNSETEIQDSQKNLAKKSGPKETIKSQAKSSSESKINQPELETRMSTRS
  SKAASNDKATKSINKNTVTVRGYSQESTKKKLSQKKLVHENPKANEQLNRRSQRLQQLTEVSRRSLRSRE
  IQGQVQAVKQSLPPTKKEQCSSTQSKSNKTSQKHVKRKVLEVKSDSKEDENLVINEVINSPKGKKRKVEH
  QTACACSSQCTQGSEKCPQKTTRRDETKPVPVTSEVKRSKMATSVVPKKNEMKKSVHTQVNTNTTLPKSP
  QPSVPEQSDNELEQAGKSKRGSILQLCEEIAGEIESDNVEVKKESSQMESVKEEKPTEIKLEETSVERQI
  LHQKETNQDVQCNRFFPSRKTKPVKCILNGINSSAKKNSNWTKIKLSKFNSVQHNKLDSQVSPKLGLLRT
  SFSPPALEMHHPVTQSTFLGTKLHDRNITCQQEKMKEINSEEVKINDITVEINKTTERAPENCHLANEIK
  PSDPPLDNQMKHSFDSASNKNFSQCLESKLENSPVENVTAASTLLSQAKIDTGENKFPGSAPQQHSILSN
  QTSKSSDNRETPRNHSLPKCNSHLEITIPKDLKLKEAEKTDEKQLIIDAGQKRFGAVSCNVCGMLYTASN
  PEDETQHLLFHNQFISAVKYVGWKKERILAEYPDGRIIMVLPEDPKYALKKVDEIREMVDNDLGFQQAPL
  MCYSRTKTLLFISNDKKVVGCLIAEHIQWGYRVIEEKLPVIRSEEEKVRFERQKAWCCSTLPEPAICGIS
  RIWVFSMMRRKKIASRMIECLRSNFIYGSYLSKEEIAFSDPTPDGKLFATQYCGTGQFLVYNFINGQNST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052911
• ORF Size: 2520 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052911.3
• RefSeq Size: 4499 bp
• RefSeq ORF: 2523 bp
• Locus ID: 114799
• UniProt ID: Q5FWF5
• Cytogenetics: 18q11.2
• MW: 95.4 kDa
• Gene Summary: ESCO1 belongs to a conserved family of acetyltransferases involved in sister chromatid cohesion (Hou and Zou, 2005 [PubMed 15958495]).[supplied by OMIM, Mar 2008]