SIGLEC6 (NM_001245) Human Tagged ORF Clone

CAT#: RC223954

SIGLEC6 (Myc-DDK-tagged)-Human sialic acid binding Ig-like lectin 6 (SIGLEC6), transcript variant 1

Product Images

Circular map for RC223954

• 

  Western blot validation of overexpression lysate (Cat# LY420052) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223954 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SIGLEC6
• Synonyms: CD33L; CD33L1; CD33L2; CD327; CDW327; OBBP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223954 representing NM_001245
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGAGCCCAGGAAGCCTCCGCCTCAGAGATGCTACCGCTGCTGCTGCCCCTGCTGTGGGCAGGGG
  CCCTGGCTCAGGAGCGGAGATTCCAGCTGGAGGGGCCAGAGTCACTGACGGTGCAGGAGGGTCTGTGCGT
  CCTCGTACCCTGCAGATTGCCCACTACCCTTCCAGCCTCGTACTATGGTTATGGCTACTGGTTCCTGGAA
  GGGGCTGATGTTCCAGTGGCCACAAACGACCCAGACGAAGAAGTGCAGGAGGAGACCCGGGGCCGATTCC
  ACCTCCTCTGGGATCCCAGAAGGAAGAACTGCTCCCTGAGCATCAGAGATGCCCGGAGGAGGGACAATGC
  TGCATACTTCTTTCGGTTGAAGTCCAAATGGATGAAATACGGTTATACATCTTCCAAGCTCTCTGTGCGT
  GTGATGGCCCTGACCCACAGGCCCAACATCTCCATCCCAGGGACCCTGGAGTCTGGCCATCCCAGCAATC
  TGACCTGCTCTGTGCCCTGGGTCTGTGAGCAGGGGACGCCCCCCATCTTCTCCTGGATGTCAGCTGCCCC
  CACCTCCCTGGGCCCCAGGACCACCCAGTCCTCGGTGCTCACAATCACCCCACGGCCCCAGGACCACAGC
  ACCAACCTCACCTGTCAGGTGACGTTCCCTGGAGCCGGTGTGACCATGGAGAGAACCATCCAGCTCAATG
  TCTCCTATGCTCCACAGAAAGTGGCCATCAGCATCTTCCAAGGAAACAGCGCAGCCTTCAAAATCCTGCA
  AAACACCTCGTCCCTCCCTGTCCTGGAGGGCCAGGCTCTGCGGCTGCTCTGTGATGCTGACGGCAACCCC
  CCTGCACACCTGAGCTGGTTCCAGGGCTTCCCCGCCCTGAACGCCACCCCCATCTCCAATACCGGGGTCC
  TGGAGCTGCCTCAAGTAGGGTCTGCAGAAGAAGGAGATTTCACCTGCCGTGCTCAGCATCCTCTGGGCTC
  CCTGCAAATCTCTCTGAGTCTCTTTGTGCATTGGAAACCAGAAGGCAGGGCTGGTGGTGTCCTGGGAGCA
  GTCTGGGGAGCTAGCATCACAACCCTGGTTTTCCTCTGTGTTTGCTTCATCTTCAGAGTGAAGACTAGAA
  GGAAGAAAGCAGCCCAGCCAGTGCAAAACACGGATGATGTGAACCCCGTCATGGTCTCAGGCTCCAGGGG
  TCATCAGCACCAGTTCCAGACAGGCATAGTTTCAGACCACCCTGCTGAGGCTGGCCCCATCTCAGAAGAT
  GAGCAGGAGCTCCACTACGCTGTCCTACACTTCCACAAGGTGCAACCTCAGGAACCAAAGGTCACCGACA
  CTGAGTACTCAGAAATCAAGATACACAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223954 representing NM_001245
  Red=Cloning site Green=Tags(s)
  
  MQGAQEASASEMLPLLLPLLWAGALAQERRFQLEGPESLTVQEGLCVLVPCRLPTTLPASYYGYGYWFLE
  GADVPVATNDPDEEVQEETRGRFHLLWDPRRKNCSLSIRDARRRDNAAYFFRLKSKWMKYGYTSSKLSVR
  VMALTHRPNISIPGTLESGHPSNLTCSVPWVCEQGTPPIFSWMSAAPTSLGPRTTQSSVLTITPRPQDHS
  TNLTCQVTFPGAGVTMERTIQLNVSYAPQKVAISIFQGNSAAFKILQNTSSLPVLEGQALRLLCDADGNP
  PAHLSWFQGFPALNATPISNTGVLELPQVGSAEEGDFTCRAQHPLGSLQISLSLFVHWKPEGRAGGVLGA
  VWGASITTLVFLCVCFIFRVKTRRKKAAQPVQNTDDVNPVMVSGSRGHQHQFQTGIVSDHPAEAGPISED
  EQELHYAVLHFHKVQPQEPKVTDTEYSEIKIHK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001245
• ORF Size: 1359 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001245.7
• RefSeq Size: 2712 bp
• RefSeq ORF: 1362 bp
• Locus ID: 946
• UniProt ID: O43699
• Cytogenetics: 19q13.41
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 49.91 kDa
• Gene Summary: This gene encodes a member of the SIGLEC (sialic acid binding immunoglobulin-like lectin) family of proteins. The encoded transmembrane receptor binds sialyl-TN glycans and leptin. Placental expression of the encoded protein is upregulated in preeclampsia. [provided by RefSeq, Jul 2016]