KCTD8 (NM_198353) Human Tagged ORF Clone

CAT#: RC223904

KCTD8 (Myc-DDK-tagged)-Human potassium channel tetramerisation domain containing 8 (KCTD8)

Product Images

Circular map for RC223904

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  Western blot validation of overexpression lysate (Cat# LY405027) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223904 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KCTD8 protein (Cat# TP323904). The protein was produced from HEK293T cells transfected with KCTD8 cDNA clone (Cat# RC223904) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCTD8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223904 representing NM_198353
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGAAGGACACGGGCAGCGGCGGCAGCACCATCCTGCCCATTAGCGAGATGGTTTCCTCGTCCA
  GCTCGCCCGGCGCGTCGGCCGCCGCCGCCCCGGGGCCCTGCGCACCCTCGCCCTTCCCTGAAGTAGTGGA
  GCTGAACGTAGGCGGCCAGGTTTATGTGACCAAGCACTCGACGCTGCTCAGCGTCCCGGACAGTACTTTG
  GCCAGCATGTTCTCGCCCTCTAGTCCCCGTGGCGGCGCCCGGCGCCGGGGCGAGCTGCCCAGGGACAGCC
  GGGCGCGCTTCTTCATCGACCGGGACGGCTTCCTTTTCAGGTACGTGCTGGATTATCTGCGGGACAAGCA
  ACTCGCGCTGCCGGAGCACTTCCCCGAGAAGGAGCGGCTGCTGCGCGAGGCCGAGTATTTCCAGCTCACC
  GACTTGGTCAAGCTGCTGTCGCCCAAGGTCACCAAGCAGAACTCTCTCAACGACGAGGGCTGCCAGAGCG
  ACCTGGAGGACAACGTCTCGCAGGGTAGCAGCGACGCGCTGCTGCTGCGCGGGGCGGCGGCCGCCGTGCC
  CTCGGGCCCGGGAGCGCACGGTGGTGGCGGCGGCGGCGGCGCGCAGGACAAGCGCTCGGGCTTCCTCACG
  CTGGGCTACCGGGGCTCCTACACCACCGTGCGCGACAACCAGGCCGACGCCAAATTCCGGCGTGTGGCGC
  GCATCATGGTGTGCGGGCGCATCGCGCTGGCCAAGGAGGTCTTCGGGGACACGCTCAACGAGAGCCGCGA
  CCCCGACCGGCAGCCGGAGAAGTACACGTCCCGCTTCTACCTCAAGTTCACCTACTTGGAGCAGGCCTTT
  GATCGCCTGTCCGAGGCCGGCTTCCACATGGTGGCGTGTAACTCCTCGGGCACCGCCGCCTTCGTCAACC
  AGTACCGCGACGACAAGATCTGGAGCAGCTACACCGAGTACATTTTCTTCCGACCACCTCAGAAAATAGT
  ATCACCTAAACAAGAACATGAAGATAGGAAACATGACAAAGTCACTGATAAAGGAAGTGAAAGTGGGACT
  TCCTGTAATGAGCTCTCCACTTCCAGTTGTGACAGCCATTCAGAGGCAAGCACTCCCCAGGACAACCCAT
  CCAGTGCCCAGCAGGCAACAGCTCACCAACCTAACACTTTAACATTGGATCGCCCCTCTAAAAAAGCACC
  TGTACAATGGATACCCCCACCAGACAAACGCAGAAACAGTGAACTCTTTCAGACCCTCATCAGCAAGTCC
  CGGGAAACAAATCTGTCCAAAAAGAAAGTCTGTGAGAAGCTAAGTGTGGAAGAAGAAATGAAAAAGTGTA
  TTCAGGATTTTAAAAAAATCCACATTCCAGATTATTTTCCAGAGCGCAAACGCCAATGGCAATCTGAACT
  GTTGCAGAAGTATGGGTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223904 representing NM_198353
  Red=Cloning site Green=Tags(s)
  
  MALKDTGSGGSTILPISEMVSSSSSPGASAAAAPGPCAPSPFPEVVELNVGGQVYVTKHSTLLSVPDSTL
  ASMFSPSSPRGGARRRGELPRDSRARFFIDRDGFLFRYVLDYLRDKQLALPEHFPEKERLLREAEYFQLT
  DLVKLLSPKVTKQNSLNDEGCQSDLEDNVSQGSSDALLLRGAAAAVPSGPGAHGGGGGGGAQDKRSGFLT
  LGYRGSYTTVRDNQADAKFRRVARIMVCGRIALAKEVFGDTLNESRDPDRQPEKYTSRFYLKFTYLEQAF
  DRLSEAGFHMVACNSSGTAAFVNQYRDDKIWSSYTEYIFFRPPQKIVSPKQEHEDRKHDKVTDKGSESGT
  SCNELSTSSCDSHSEASTPQDNPSSAQQATAHQPNTLTLDRPSKKAPVQWIPPPDKRRNSELFQTLISKS
  RETNLSKKKVCEKLSVEEEMKKCIQDFKKIHIPDYFPERKRQWQSELLQKYGL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198353
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198353.3
• RefSeq Size: 2240 bp
• RefSeq ORF: 1422 bp
• Locus ID: 386617
• UniProt ID: Q6ZWB6
• Cytogenetics: 4p13
• MW: 52.3 kDa
• Gene Summary: Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization (By similarity).[UniProtKB/Swiss-Prot Function]