RHOBTB1 (NM_001032380) Human Tagged ORF Clone

CAT#: RC223885

RHOBTB1 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 1 (RHOBTB1), transcript variant 3

Product Images

Circular map for RC223885

• 

  Western blot validation of overexpression lysate (Cat# LY422316) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223885 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RHOBTB1
• Synonyms: 1700008H16Rik; 3110048G13Rik; AI173445; AI573858; AV350930; KIAA0740, MGC33059, MGC33841; MGC107538; Rho-related BTB domain containing 1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223885 representing NM_001032380
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCTGACATGGACTACGAAAGACCCAACGTTGAAACTATCAAATGTGTGGTCGTGGGTGACAATG
  CCGTGGGGAAGACGCGCTTGATCTGTGCCAGGGCGTGCAACACCACACTCACGCAGTATCAGCTGCTGGC
  CACCCACGTGCCAACAGTGTGGGCGATTGACCAGTACCGCGTGTGCCAGGAGGTCTTGGAGCGTTCTCGG
  GATGTTGTTGATGAAGTGAGTGTTTCTCTCAGGCTTTGGGATACTTTTGGTGATCATCACAAAGACAGAC
  GCTTTGCATATGGCAGGTCTGATGTTGTGGTCCTCTGTTTTTCGATTGCTAATCCCAATTCCCTAAATCA
  TGTGAAAAGCATGTGGTATCCAGAAATCAAGCACTTTTGCCCTCGAACACCCGTTATCCTTGTTGGGTGC
  CAGCTTGATCTCCGCTATGCCGACCTGGAAGCTGTTAATCGAGCCAGGCGCCCGTTAGCAAGGCCCATAA
  AGAGAGGGGATATTTTGCCCCCAGAAAAAGGCCGAGAGGTAGCAAAGGAACTTGGCTTACCATACTATGA
  AACAAGCGTGTTTGACCAGTTTGGTATCAAGGATGTGTTTGACAATGCAATCCGAGCAGCGCTGATTTCC
  CGCAGGCACCTGCAATTCTGGAAATCCCACCTAAAGAAAGTCCAGAAACCTTTACTTCAGGCACCCTTCC
  TACCTCCAAAAGCCCCTCCACCGGTCATCAAAATTCCAGAGTGTCCTTCCATGGGGACAAATGAAGCTGC
  CTGTTTACTGGACAATCCTCTATGTGCCGATGTTCTGTTCATCCTTCAGGACCAGGAACACATCTTTGCA
  CATCGAATTTACCTCGCTACCTCTTCTTCCAAATTTTATGATCTGTTTTTAATGGAATGTGAAGAATCCC
  CAAATGGGAGTGAAGGAGCCTGTGAGAAAGAGAAGCAGAGCAGAGATTTCCAGGGGCGGATATTGAGTGT
  CGACCCAGAGGAAGAAAGGGAGGAGGGCCCGCCTAGGATTCCTCAGGCCGACCAGTGGAAGTCTTCAAAC
  AAGAGCCTGGTGGAGGCTCTGGGGCTGGAAGCCGAGGGTGCAGTTCCTGAGACACAGACTTTGACCGGAT
  GGAGTAAGGGGTTCATTGGCATGCACAGGGAAATGCAAGTCAACCCCATTTCAAAGCGGATGGGGCCCAT
  GACTGTGGTCAGGATGGACGCTTCAGTCCAGCCAGGCCCTTTTCGGACCCTGCTCCAGTTTCTTTATACG
  GGACAACTGGATGAAAAGGAAAAGGATTTGGTGGGCCTGGCTCAGATCGCAGAGGTCCTCGAGATGTTCG
  ATTTGAGGATGATGGTGGAAAACATCATGAACAAGGAAGCCTTCATGAACCAGGAGATTACGAAAGCCTT
  TCACGTAAGGAAAGCCAATCGGATAAAAGAGTGTCTCAGCAAGGGAACGTTCTCGGACGTGACATTTAAA
  TTGGACGATGGAGCCATCAGTGCCCACAAGCCGCTGCTGATCTGTAGCTGTGAGTGGATGGCAGCCATGT
  TCGGGGGGTCATTTGTGGAAAGTGCCAACAGTGAGGTGTATCTCCCGAACATAAACAAGATATCAATGCA
  AGCAGTATTGGATTATCTCTATACCAAGCAGTTGTCTCCTAACTTGGATCTGGACCCGCTGGAATTAATT
  GCCTTGGCAAACAGATTTTGCCTGCCACACTTGGTTGCACTTGCAGAACAGCATGCCGTTCAGGAGTTGA
  CCAAAGCCGCCACGAGTGGCGTGGGCATTGACGGAGAAGTGCTCTCTTACTTGGAATTGGCTCAGTTTCA
  CAATGCCCACCAGTTGGCCGCCTGGTGTTTGCACCACATCTGCACCAACTACAACAGTGTATGCTCCAAG
  TTCCGTAAGGAAATCAAATCAAAATCTGCAGACAACCAGGAATACTTCGAGCGGCACCGCTGGCCCCCTG
  TGTGGTACCTGAAGGAAGAAGATCACTACCAGCGTGTGAAAAGGGAACGAGAGAAGGAAGATATTGCACT
  AAATAAGCATCGCTCAAGACGAAAGTGGTGCTTCTGGAATTCATCTCCAGCAGTGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223885 representing NM_001032380
  Red=Cloning site Green=Tags(s)
  
  MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYRVCQEVLERSR
  DVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKSMWYPEIKHFCPRTPVILVGC
  QLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKELGLPYYETSVFDQFGIKDVFDNAIRAALIS
  RRHLQFWKSHLKKVQKPLLQAPFLPPKAPPPVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFA
  HRIYLATSSSKFYDLFLMECEESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQADQWKSSN
  KSLVEALGLEAEGAVPETQTLTGWSKGFIGMHREMQVNPISKRMGPMTVVRMDASVQPGPFRTLLQFLYT
  GQLDEKEKDLVGLAQIAEVLEMFDLRMMVENIMNKEAFMNQEITKAFHVRKANRIKECLSKGTFSDVTFK
  LDDGAISAHKPLLICSCEWMAAMFGGSFVESANSEVYLPNINKISMQAVLDYLYTKQLSPNLDLDPLELI
  ALANRFCLPHLVALAEQHAVQELTKAATSGVGIDGEVLSYLELAQFHNAHQLAAWCLHHICTNYNSVCSK
  FRKEIKSKSADNQEYFERHRWPPVWYLKEEDHYQRVKREREKEDIALNKHRSRRKWCFWNSSPAVA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001032380
• ORF Size: 2088 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001032380.1, NP_001027552.1
• RefSeq Size: 4381 bp
• RefSeq ORF: 2090 bp
• Locus ID: 9886
• Cytogenetics: 10q21.2
• MW: 79.9 kDa
• Gene Summary: The protein encoded by this gene belongs to the Rho family of the small GTPase superfamily. It contains a GTPase domain, a proline-rich region, a tandem of 2 BTB (broad complex, tramtrack, and bric-a-brac) domains, and a conserved C-terminal region. The protein plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]