FUT4 (NM_002033) Human Tagged ORF Clone

CAT#: RC223829

FUT4 (Myc-DDK-tagged)-Human fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific) (FUT4)

Product Images

Circular map for RC223829

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  Western blot validation of overexpression lysate (Cat# LY419574) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223829 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FUT4
• Synonyms: CD15; ELFT; FCT3A; FUC-TIV; FUTIV; LeX; SSEA-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223829 representing NM_002033
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGCTTGTGGGGCGCGGCCCGGAAGCCCTCGGGCGCGGGCTGGGAGAAGGAGTGGGCGGAGGCGC
  CGCAGGAGGCTCCCGGGGCCTGGTCGGGCCGGCTGGGCCCCGGGCGCAGTGGAAGAAAGGGACGGGCGGT
  GCCCGGTTGGGCGTCCTGGCCAGCTCACCTTGCCCTGGCGGCTCGCCCCGCCCGGCACTTGGGAGGAGCA
  GGGCAGGGCCCGCGGCCTTTGCATTCTGGGACCGCCCCCTTCCATTCCCGGGCCAGCGGCGAGCGGCAGC
  GACGGCTGGAGCCGCAGCTACAGCATGAGAGCCGGTGCCGCTCCTCCACGCCTGCGGACGCGTGGCGAGC
  GGAGGCAGCGCTGCCTGTTCGCGCCATGGGGGCACCGTGGGGCTCGCCGACGGCGGCGGCGGGCGGGCGG
  CGCGGGTGGCGCCGAGGCCGGGGGCTGCCATGGACCGTCTGTGTGCTGGCGGCCGCCGGCTTGACGTGTA
  CGGCGCTGATCACCTACGCTTGCTGGGGGCAGCTGCCGCCGCTGCCCTGGGCGTCGCCAACCCCGTCGCG
  ACCGGTGGGCGTGCTGCTGTGGTGGGAGCCCTTCGGGGGGCGCGATAGCGCCCCGAGGCCGCCCCCTGAC
  TGCCGGCTGCGCTTCAACATCAGCGGCTGCCGCCTGCTCACCGACCGCGCGTCCTACGGAGAGGCTCAGG
  CCGTGCTTTTCCACCACCGCGACCTCGTGAAGGGGCCCCCCGACTGGCCCCCGCCCTGGGGCATCCAGGC
  GCACACTGCCGAGGAGGTGGATCTGCGCGTGTTGGACTACGAGGAGGCAGCGGCGGCGGCAGAAGCCCTG
  GCGACCTCCAGCCCCAGGCCCCCGGGCCAGCGCTGGGTTTGGATGAACTTCGAGTCGCCCTCGCACTCCC
  CGGGGCTGCGAAGCCTGGCAAGTAACCTCTTCAACTGGACGCTCTCCTACCGGGCGGACTCGGACGTCTT
  TGTGCCTTATGGCTACCTCTACCCCAGAAGCCACCCCGGCGACCCGCCCTCAGGCCTGGCCCCGCCACTG
  TCCAGGAAACAGGGGCTGGTGGCATGGGTGGTGAGCCACTGGGACGAGCGCCAGGCCCGGGTCCGCTACT
  ACCACCAACTGAGCCAACATGTGACCGTGGACGTGTTCGGCCGGGGCGGGCCGGGGCAGCCGGTGCCCGA
  AATTGGGCTCCTGCACACAGTGGCCCGCTACAAGTTCTACCTGGCTTTCGAGAACTCGCAGCACCTGGAT
  TATATCACCGAGAAGCTCTGGCGCAACGCGTTGCTCGCTGGGGCGGTGCCGGTGGTGCTGGGCCCAGACC
  GTGCCAACTACGAGCGCTTTGTGCCCCGCGGCGCCTTCATCCACGTGGACGACTTCCCAAGTGCCTCCTC
  CCTGGCCTCGTACCTGCTTTTCCTCGACCGCAACCCCGCGGTCTATCGCCGCTACTTCCACTGGCGCCGG
  AGCTACGCTGTCCACATCACCTCCTTCTGGGACGAGCCTTGGTGCCGGGTGTGCCAGGCTGTACAGAGGG
  CTGGGGACCGGCCCAAGAGCATACGGAACTTGGCCAGCTGGTTCGAGCGG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223829 representing NM_002033
  Red=Cloning site Green=Tags(s)
  
  MRRLWGAARKPSGAGWEKEWAEAPQEAPGAWSGRLGPGRSGRKGRAVPGWASWPAHLALAARPARHLGGA
  GQGPRPLHSGTAPFHSRASGERQRRLEPQLQHESRCRSSTPADAWRAEAALPVRAMGAPWGSPTAAAGGR
  RGWRRGRGLPWTVCVLAAAGLTCTALITYACWGQLPPLPWASPTPSRPVGVLLWWEPFGGRDSAPRPPPD
  CRLRFNISGCRLLTDRASYGEAQAVLFHHRDLVKGPPDWPPPWGIQAHTAEEVDLRVLDYEEAAAAAEAL
  ATSSPRPPGQRWVWMNFESPSHSPGLRSLASNLFNWTLSYRADSDVFVPYGYLYPRSHPGDPPSGLAPPL
  SRKQGLVAWVVSHWDERQARVRYYHQLSQHVTVDVFGRGGPGQPVPEIGLLHTVARYKFYLAFENSQHLD
  YITEKLWRNALLAGAVPVVLGPDRANYERFVPRGAFIHVDDFPSASSLASYLLFLDRNPAVYRRYFHWRR
  SYAVHITSFWDEPWCRVCQAVQRAGDRPKSIRNLASWFER
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002033
• ORF Size: 1590 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002033.3
• RefSeq Size: 5937 bp
• RefSeq ORF: 1593 bp
• Locus ID: 2526
• UniProt ID: P22083
• Cytogenetics: 11q21
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
• MW: 58.9 kDa
• Gene Summary: The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]