GSDMC (NM_031415) Human Tagged ORF Clone

CAT#: RC223817

GSDMC (Myc-DDK-tagged)-Human gasdermin C (GSDMC)

Product Images

Circular map for RC223817

• 

  Western blot validation of overexpression lysate (Cat# LY410540) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223817 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GSDMC
• Synonyms: MLZE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223817 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTCCATGTTGGAACGCATTAGCAAAAATTTGGTCAAAGAGATTGGAAGCAAAGACCTGACATCTG
  TCAAATACCTATTGAGTGCCACCAAATTACGTCAGTTTGTTATATTACGAAAGAAGAAGGATTCTCGTTC
  ATCATTTTGGGAACAATCTGACTATGTTCCAGTTGAATTCTCCCTCAATGACATCCTGGAGCCAAGTTCT
  TCAGTCCTAGAAACTGTTGTGACAGGACCGTTCCACTTCAGTGACATTATGATCCAGAAGCATAAGGCTG
  ACATGGGTGTGAATGTTGGTATAGAAGTGAGTGTGTCAGGGGAGGCCTCTGTGGACCATGGATGCTCCCT
  CGAGTTTCAAATTGTTACCATCCCATCACCAAACCTGGAAGACTTTCAAAAAAGGAAACTGTTGGATCCA
  GAGCCATCATTTCTGAAGGAGTGCCGGAGGAGAGGGGACAACCTGTACGTGGTGACAGAGGCTGTTGAAC
  TGATCAACAATACTGTGCTGTACGATAGCAGTAGTGTGAATATTTTAGGGAAAATTGCTCTTTGGATTAC
  CTATGGCAAGGGTCAAGGCCAAGGAGAGAGTCTCAGAGTGAAGAAGAAGGCGCTGACTCTTCAGAAAGGC
  ATGGTGATGGCTTATAAGAGAAAGCAGCTGGTTATCAAGGAGAAAGCCATTCTCATCTCAGATGATGATG
  AACAGAGAACCTTTCAAGATGAGTACGAAATTTCCGAAATGGTAGGCTACTGTGCTGCGAGGAGTGAGGG
  GTTGCTACCATCATTTCATACCATCTCTCCAACCCTCTTCAATGCCTCATCCAATGATATGAAGTTAAAA
  CCAGAGCTATTTCTGACACAGCAATTTTTGAGCGGGCATTTGCCAAAATACGAACAAGTTCACATCCTCC
  CAGTAGGAAGAATAGAGGAACCCTTCTGGCAAAATTTCAAGCATCTACAAGAGGAGGTTTTCCAGAAAAT
  AAAGACACTGGCTCAGCTCTCAAAGGATGTTCAGGATGTCATGTTCTACAGTATCCTGGCCATGCTCAGA
  GACAGAGGGGCTCTACAGGACCTGATGAACATGCTGGAATTGGACAGCTCAGGTCATTTGGATGGCCCTG
  GTGGTGCCATCCTAAAGAAACTTCAACAGGATTCAAACCATGCATGGTTTAACCCAAAGGACCCCATTCT
  TTATCTCCTTGAAGCCATAATGGTGCTGAGTGACTTCCAACACGATTTGCTGGCCTGTTCCATGGAGAAG
  AGGATCCTGCTTCAGCAACAGGAGCTGGTAAGGAGCATCCTGGAGCCAAACTTCAGATACCCCTGGAGCA
  TTCCCTTCACCCTCAAACCTGAGCTCCTCGCCCCACTCCAGAGTGAGGGTTTGGCCATCACCTATGGCCT
  GCTGGAGGAGTGTGGCCTTAGGACGGAGCTGGATAACCCCAGGTCAACCTGGGATGTAGAAGCAAAGATG
  CCCCTGTCTGCCCTCTATGGGACTCTCTCATTGCTGCAGCAGCTGGCTGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223817 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPSMLERISKNLVKEIGSKDLTSVKYLLSATKLRQFVILRKKKDSRSSFWEQSDYVPVEFSLNDILEPSS
  SVLETVVTGPFHFSDIMIQKHKADMGVNVGIEVSVSGEASVDHGCSLEFQIVTIPSPNLEDFQKRKLLDP
  EPSFLKECRRRGDNLYVVTEAVELINNTVLYDSSSVNILGKIALWITYGKGQGQGESLRVKKKALTLQKG
  MVMAYKRKQLVIKEKAILISDDDEQRTFQDEYEISEMVGYCAARSEGLLPSFHTISPTLFNASSNDMKLK
  PELFLTQQFLSGHLPKYEQVHILPVGRIEEPFWQNFKHLQEEVFQKIKTLAQLSKDVQDVMFYSILAMLR
  DRGALQDLMNMLELDSSGHLDGPGGAILKKLQQDSNHAWFNPKDPILYLLEAIMVLSDFQHDLLACSMEK
  RILLQQQELVRSILEPNFRYPWSIPFTLKPELLAPLQSEGLAITYGLLEECGLRTELDNPRSTWDVEAKM
  PLSALYGTLSLLQQLAEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031415
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031415.3
• RefSeq Size: 2731 bp
• RefSeq ORF: 1527 bp
• Locus ID: 56169
• UniProt ID: Q9BYG8
• Cytogenetics: 8q24.21
• Domains: DFNA5
• MW: 57.7 kDa
• Gene Summary: The N-terminal moiety promotes pyroptosis. May be acting by homooligomerizing within the membrane and forming pores (PubMed:27281216). The physiological relevance of this observation is unknown (Probable).[UniProtKB/Swiss-Prot Function]