PMFBP1 (NM_031293) Human Tagged ORF Clone

CAT#: RC223808

PMFBP1 (Myc-DDK-tagged)-Human polyamine modulated factor 1 binding protein 1 (PMFBP1), transcript variant 1

Product Images

Circular map for RC223808

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PMFBP1
• Synonyms: SPGF31; STAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223808 representing NM_031293
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGATGAGGCGGGGGAGAGAGACAGAGAAGTGAGCAGCCTGAACAGCAAGCTGTTAAGCCTGCAAC
  TTGACATCAAGAATCTGCACGATGTCTGCAAGAGACAGAGGAAGACCTTGCAGGACAATCAGCTCTGCAT
  GGAGGAGGCAATGAACAGCAGCCACGACAAGAAGCAAGCACAGGCATTAGCATTCGAGGAGTCAGAGGTG
  GAATTTGGGTCCAGTAAACAGTGTCATCTGAGACAACTCCAGCAACTGAAGAAAAAATTGCTGGTCCTTC
  AACAAGAACTGGAGTTTCACACAGAGGAGTTGCAGACTTCTTACTATTCTCTCCGCCAGTATCAGTCCAT
  CCTAGAGAAGCAGACTTCCGACCTGGTTCTTCTGCACCATCACTGCAAACTGAAAGAAGATGAGGTGATT
  CTCTATGAGGAGGAAATGGGAAATCACAACGAGAACACAGGGGAGAAGCTCCATTTGGCGCAGGAGCAAC
  TCGCCTTGGCCGGGGACAAGATCGCCTCTCTAGAGAGGAGCTTAAACCTCTACAGGGATAAATACCAGTC
  TTCCCTGAGCAACATCGAGTTACTAGAATGCCAAGTGAAGATGTTGCAGGGGGAACTCGGCGGGATCATG
  GGTCAGGAGCCTGAGAACAAGGGTGATCATTCAAAGGTACGGATATACACTTCTCCTTGCATGATTCAAG
  AGCATCAGGAGACTCAGAAACGACTGTCTGAAGTCTGGCAAAAGGTCTCTCAACAGGATGATCTCATTCA
  AGAACTTCGAAATAAGCTGGCCTGCAGTAACGCTTTGGTTCTGGAGCGTGAAAAGGCTTTGATAAAACTA
  CAAGCCGATTTTGCTTCCTGTACAGCCACCCACAGATACCCTCCTAGCTCCTCAGAAGAGTGTGAAGACA
  TCAAAAAGATACTGAAGCACTTGCAGGAGCAGAAAGACAGCCAGTGCCTGCATGTGGAGGAGTACCAGAA
  CCTGGTGAAGGATCTGCGCGTGGAACTAGAGGCCGTGTCGGAACAGAAGAGAAACATCATGAAGGACATG
  ATGAAGCTGGAGCTGGACCTGCACGGACTGCGGGAGGAGACATCTGCCCACATTGAGAGGAAGGATAAGG
  ACATCACCATCCTGCAGTGCCGGCTGCAGGAGCTGCAGCTGGAGTTCACCGAGACCCAAAAGCTCACTTT
  GAAGAAAGACAAGTTCCTCCAAGAGAAAGATGAGATGCTGCAAGAGCTGGAGAAGAAACTGACACAGGTT
  CAGAACAGCCTCCTGAAAAAGGAGAAGGAGCTGGAGAAGCAGCAGTGCATGGCCACAGAACTTGAAATGA
  CAGTCAAGGAGGCTAAGCAGGACAAGTCCAAGGAGGCGGAGTGCAAGGCCCTGCAGGCTGAGGTCCAGAA
  GCTGAAGAACAGTCTCGAAGAGGCCAAGCAGCAGGAGAGGCTGGCTGCTCAGCAAGCAGCCCAGTGCAAA
  GAAGAGGCTGCACTGGCAGGCTGTCACCTGGAGGACACCCAGAGGAAACTGCAGAAGGGTCTCCTCCTGG
  ACAAGCAGAAGGCAGACACCATCCAGGAACTACAGAGAGAACTTCAGATGCTGCAGAAGGAGTCCTCGAT
  GGCTGAGAAGGAACAAACCTCCAACAGAAAACGGGTGGAGGAGCTGTCATTAGAACTCTCTGAAGCCCTG
  AGGAAGCTTGAAAATTCAGACAAGGAAAAGAGGCAGCTTCAGAAGACAGTGGCTGAGCAGGATATGAAAA
  TGAATGACATGCTTGATCGTATCAAGCACCAGCACAGGGAGCAAGGCTCCATCAAATGCAAGTTAGAAGA
  AGATCTTCAGGAGGCCACAAAGCTTCTGGAGGACAAACGGGAGCAGTTGAAGAAGAGCAAAGAGCATGAG
  AAGCTGATGGAGGGAGAACTTGAAGCTTTGCGGCAGGAATTTAAAAAGAAAGACAAGACGTTGAAAGAGA
  ATTCCAGAAAGTTGGAGGAAGAAAATGAGAATCTCCGAGCAGAGCTACAGTGTTGTTCTACACAACTGGA
  ATCCTCTCTCAACAAATACAACACCAGCCAGCAAGTCATCCAAGACTTGAATAAAGAGATAGCCCTTCAG
  AAGGAGTCCTTAATGAGCCTGCAGGCCCAGCTGGACAAAGCTCTGCAGAAGGAGAAGCACTATCTCCAGA
  CTACCATCACCAAAGAAGCCTATGATGCATTATCCCGGAAGTCAGCCGCCTGCCAGGATGACCTGACACA
  AGCCCTCGAGAAGCTCAATCACGTGACCTCAGAGACAAAGAGCCTGCAGCAAAGCTTGACACAGACCCAA
  GAGAAGAAAGCTCAGCTGGAAGAGGAAATCATTGCTTATGAGGAAAGGATGAAAAAGCTCAATACGGAAT
  TAAGAAAACTGCGGGGCTTCCACCAGGAGAGTGAGCTGGAGGTGCACGCCTTTGACAAGAAGCTAGAGGA
  GATGAGCTGCCAGGTGCTGCAGTGGCAGAAGCAACACCAGAATGACCTCAAGATGCTGGCAGCCAAAGAG
  GAGCAGCTCAGGGAGTTCCAGGAGGAGATGGCCGCCTTAAAAGAGAACCTCCTTGAGGACGATAAGGAGC
  CCTGCTGCCTGCCCCAGTGGTCTGTGCCCAAAGACACCTGTAGGCTCTACCGAGGGAATGATCAGATTAT
  GACCAACTTGGAGCAATGGGCAAAACAGCAGAAGGTCGCCAATGAGAAACTAGGAAACCAGCTCCGAGAG
  CAGGTGAAATACATTGCCAAGCTGAGTGGCGAAAAGGACCACCTCCACAGTGTAATGGTCCACTTGCAGC
  AGGAAAACAAGAAGCTGAAGAAGGAGATAGAAGAGAAGAAGATGAAAGCCGAGAACACAAGGCTATGCAC
  CAAAGCCCTAGGCCCGAGCAGAACGGAGTCCACACAGAGGGAGAAAGTGTGCGGCACCTTGGGCTGGAAG
  GGGTTGCCCCAGGATATGGGTCAAAGAATGGACCTCACCAAGTACATCGGGATGCCCCACTGCCCGGGTT
  CCTCATACTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223808 representing NM_031293
  Red=Cloning site Green=Tags(s)
  
  MKDEAGERDREVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQLCMEEAMNSSHDKKQAQALAFEESEV
  EFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSILEKQTSDLVLLHHHCKLKEDEVI
  LYEEEMGNHNENTGEKLHLAQEQLALAGDKIASLERSLNLYRDKYQSSLSNIELLECQVKMLQGELGGIM
  GQEPENKGDHSKVRIYTSPCMIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKL
  QADFASCTATHRYPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVSEQKRNIMKDM
  MKLELDLHGLREETSAHIERKDKDITILQCRLQELQLEFTETQKLTLKKDKFLQEKDEMLQELEKKLTQV
  QNSLLKKEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAEVQKLKNSLEEAKQQERLAAQQAAQCK
  EEAALAGCHLEDTQRKLQKGLLLDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEAL
  RKLENSDKEKRQLQKTVAEQDMKMNDMLDRIKHQHREQGSIKCKLEEDLQEATKLLEDKREQLKKSKEHE
  KLMEGELEALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSLNKYNTSQQVIQDLNKEIALQ
  KESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAACQDDLTQALEKLNHVTSETKSLQQSLTQTQ
  EKKAQLEEEIIAYEERMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKE
  EQLREFQEEMAALKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLRE
  QVKYIAKLSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAENTRLCTKALGPSRTESTQREKVCGTLGWK
  GLPQDMGQRMDLTKYIGMPHCPGSSYC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031293
• ORF Size: 3021 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031293.3
• RefSeq Size: 3405 bp
• RefSeq ORF: 3024 bp
• Locus ID: 83449
• UniProt ID: Q8TBY8
• Cytogenetics: 16q22.2
• MW: 117.3 kDa
• Gene Summary: Required for normal spermatogenesis (PubMed:1770140, PubMed:30032984, PubMed:30298696). It functions as a scaffold protein that attaches the sperm head-tail connecting piece to the nuclear envelope, thus maintaining sperm head and tail integrity (PubMed:30032984). May also be involved in the general organization of cellular cytoskeleton (By similarity).[UniProtKB/Swiss-Prot Function]