PIP5K1 beta (PIP5K1B) (NM_003558) Human Tagged ORF Clone

CAT#: RC223713

PIP5K1B (Myc-DDK-tagged)-Human phosphatidylinositol-4-phosphate 5-kinase, type I, beta (PIP5K1B), transcript variant 2

Product Images

Circular map for RC223713

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  Western blot validation of overexpression lysate (Cat# LY418599) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223713 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PIP5K1B protein (Cat# TP323713). The protein was produced from HEK293T cells transfected with PIP5K1B cDNA clone (Cat# RC223713) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PIP5K1 beta
• Synonyms: MSS4; STM7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223713 representing NM_003558
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCTGCTGCTGAAAATGGAGAGGCAGCACCTGGAAAACAAAATGAAGAAAAAACCTATAAAAAGA
  CTGCATCATCTGCTATTAAAGGTGCTATTCAGCTGGGAATAGGATACACAGTGGGTAATCTCACTTCCAA
  GCCAGAACGAGATGTTCTTATGCAAGACTTTTATGTGGTGGAAAGTGTGTTCCTACCCAGCGAAGGGAGC
  AATCTGACCCCAGCACATCACTACCCAGACTTTAGATTTAAGACATACGCTCCATTAGCATTCCGATATT
  TCAGAGAACTTTTTGGTATCAAGCCTGATGATTACTTGTATTCCATCTGCAGTGAACCTCTAATAGAACT
  GTCTAACCCTGGAGCCAGTGGATCCTTGTTTTTTGTGACCAGTGATGATGAATTTATCATCAAAACAGTT
  CAGCACAAAGAAGCTGAGTTTCTTCAGAAGCTACTGCCAGGCTATTACATGAATTTAAACCAGAATCCAA
  GGACTCTTTTGCCAAAATTTTACGGACTGTATTGTATGCAATCAGGAGGCATTAATATCAGGATTGTGGT
  GATGAACAACGTTTTGCCACGCTCCATGAGAATGCACTTTACATATGACTTGAAAGGCTCAACGTATAAG
  CGAAGAGCATCCCGTAAAGAGAGAGAGAAATCCAACCCCACATTTAAGGACTTAGATTTCCTGCAAGACA
  TGCACGAAGGGTTGTATTTTGATACGGAAACATACAACGCGCTTATGAAAACACTTCAGAGAGACTGCCG
  GGTGCTAGAAAGCTTCAAGATCATGGATTATAGCCTTCTGTTGGGAATTCATTTCCTGGACCATTCCCTC
  AAAGAGAAAGAGGAGGAGACCCCACAAAATGTGCCTGATGCTAAGCGGACTGGGATGCAGAAGGTTCTCT
  ACTCAACAGCCATGGAATCTATCCAGGGTCCAGGGAAATCTGGAGATGGGATAATCACAGAGAACCCAGA
  CACAATGGGAGGCATTCCAGCTAAAAGCCATAGGGGAGAAAAACTACTTTTATTTATGGGCATTATTGAC
  ATTCTGCAATCATATAGGTTAATGAAGAAGTTAGAACATTCCTGGAAAGCTCTTGTTTATGATGGGGACA
  CTGTTTCTGTTCATAGACCAAGCTTTTATGCAGACAGATTTCTTAAGTTCATGAATTCCAGAGTTTTCAA
  GAAAATTCAAGCTTTGAAGGCTTCACCGTCTAAGAAACGGTGCAATTCAATCGCCGCCCTAAAGGCCACT
  TCACAGGAGATTGTGTCCTCAATTAGCCAGGAATGGAAGGATGAGAAGCGGGATTTGCTGACTGAAGGAC
  AAAGTTTTAGCAGCCTTGATGAAGAAGCCCTGGGATCCCGACACAGGCCAGACCTGGTCCCTAGCACTCC
  ATCACTGTTTGAAGCTGCTTCCTTGGCAACCACAATTTCATCTTCTTCCTTATACGTCAATGAGCACTAT
  CCACACGACAGGCCTACACTCTATTCAAACAGCAAAGGGTTACCTTCCAGTTCAACATTTACCTTGGAAG
  AGGGGACCATCTACTTGACCGCTGAGCCCAACACTCTGGAAGTGCAGGATGACAATGCTTCTGTGCTTGA
  CGTCTATTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223713 representing NM_003558
  Red=Cloning site Green=Tags(s)
  
  MSSAAENGEAAPGKQNEEKTYKKTASSAIKGAIQLGIGYTVGNLTSKPERDVLMQDFYVVESVFLPSEGS
  NLTPAHHYPDFRFKTYAPLAFRYFRELFGIKPDDYLYSICSEPLIELSNPGASGSLFFVTSDDEFIIKTV
  QHKEAEFLQKLLPGYYMNLNQNPRTLLPKFYGLYCMQSGGINIRIVVMNNVLPRSMRMHFTYDLKGSTYK
  RRASRKEREKSNPTFKDLDFLQDMHEGLYFDTETYNALMKTLQRDCRVLESFKIMDYSLLLGIHFLDHSL
  KEKEEETPQNVPDAKRTGMQKVLYSTAMESIQGPGKSGDGIITENPDTMGGIPAKSHRGEKLLLFMGIID
  ILQSYRLMKKLEHSWKALVYDGDTVSVHRPSFYADRFLKFMNSRVFKKIQALKASPSKKRCNSIAALKAT
  SQEIVSSISQEWKDEKRDLLTEGQSFSSLDEEALGSRHRPDLVPSTPSLFEAASLATTISSSSLYVNEHY
  PHDRPTLYSNSKGLPSSSTFTLEEGTIYLTAEPNTLEVQDDNASVLDVYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003558
• ORF Size: 1620 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003558.4
• RefSeq Size: 2764 bp
• RefSeq ORF: 1623 bp
• Locus ID: 8395
• UniProt ID: O14986
• Cytogenetics: 9q21.11
• Domains: PIP5K
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
• MW: 60.9 kDa
• Gene Summary: Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2. Together with PIP5K1A is required after stimulation of G-protein coupled receptors for stable platelet adhesion (By similarity).[UniProtKB/Swiss-Prot Function]