PIP5K1 beta (PIP5K1B) (NM_003558) Human Untagged Clone

CAT#: SC117935

PIP5K1B (untagged)-Human phosphatidylinositol-4-phosphate 5-kinase, type I, beta (PIP5K1B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIP5K1 beta
• Synonyms: MSS4; STM7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117935 sequence for NM_003558 edited (data generated by NextGen Sequencing)
  ATGTCTTCTGCTGCTGAAAATGGAGAGGCAGCACCTGGAAAACAAAATGAAGAAAAAACC
  TATAAAAAGACTGCATCATCTGCTATTAAAGGTGCTATTCAGCTGGGAATAGGATACACA
  GTGGGTAATCTCACTTCCAAGCCAGAACGAGATGTTCTTATGCAAGACTTTTATGTGGTG
  GAAAGTGTGTTCCTACCCAGCGAAGGGAGCAATCTGACCCCAGCACATCACTACCCAGAC
  TTTAGATTTAAGACATACGCTCCATTAGCATTCCGATATTTCAGAGAACTTTTTGGTATC
  AAGCCTGATGATTACTTGTATTCCATCTGCAGTGAACCTCTAATAGAACTGTCTAACCCT
  GGAGCCAGTGGATCCTTGTTTTTTGTGACCAGTGATGATGAATTTATCATCAAAACAGTT
  CAGCACAAAGAAGCTGAGTTTCTTCAGAAGCTACTGCCAGGCTATTACATGAATTTAAAC
  CAGAATCCAAGGACTCTTTTGCCAAAATTTTACGGACTGTATTGTATGCAATCAGGAGGC
  ATTAATATCAGGATTGTGGTGATGAACAACGTTTTGCCACGCTCCATGAGAATGCACTTT
  ACATATGACTTGAAAGGCTCAACGTATAAGCGAAGAGCATCCCGTAAAGAGAGAGAGAAA
  TCCAACCCCACATTTAAGGACTTAGATTTCCTGCAAGACATGCACGAAGGGTTGTATTTT
  GATACGGAAACATACAACGCGCTTATGAAAACACTTCAGAGAGACTGCCGGGTGCTAGAA
  AGCTTCAAGATCATGGATTATAGCCTTCTGTTGGGAATTCATTTCCTGGACCATTCCCTC
  AAAGAGAAAGAGGAGGAGACCCCACAAAATGTGCCTGATGCTAAGCGGACTGGGATGCAG
  AAGGTTCTCTACTCAACAGCCATGGAATCTATCCAGGGTCCAGGGAAATCTGGAGATGGG
  ATAATCACAGAGAACCCAGACACAATGGGAGGCATTCCAGCTAAAAGCCATAGGGGAGAA
  AAACTACTTTTATTTATGGGCATTATTGACATTCTGCAATCATATAGGTTAATGAAGAAG
  TTAGAACATTCCTGGAAAGCTCTTGTTTATGATGGGGACACTGTTTCTGTTCATAGACCA
  AGCTTTTATGCAGACAGATTTCTTAAGTTCATGAATTCCAGAGTTTTCAAGAAAATTCAA
  GCTTTGAAGGCTTCACCGTCTAAGAAACGGTGCAATTCAATCGCCGCCCTAAAGGCCACT
  TCACAGGAGATTGTGTCCTCAATTAGCCAGGAATGGAAGGATGAGAAGCGGGATTTGCTG
  ACTGAAGGACAAAGTTTTAGCAGCCTTGATGAAGAAGCCCTGGGATCCCGACACAGGCCA
  GACCTGGTCCCTAGCACTCCATCACTGTTTGAAGCTGCTTCCTTGGCAACCACAATTTCA
  TCTTCTTCCTTATACGTCAATGAGCACTATCCACACGACAGGCCTACACTCTATTCAAAC
  AGCAAAGGGTTACCTTCCAGTTCAACATTTACCTTGGAAGAGGGGACCATCTACTTGACC
  GCTGAGCCCAACACTCTGGAAGTGCAGGATGACAATGCTTCTGTGCTTGACGTCTATTTA
  TAA
  
  Clone variation with respect to NM_003558.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003558 unedited
  TTTAGATTTGTATCCCATTATATAGGCGGCCGCGCAATTCGCACGAGGCACTGCTCTCCC
  TTCGCTGTGGGGAAGCGACAACGTCCCGATAACTTGCAGACTGTGGCGCAACTGGTCTTG
  GTAGCGGAGGCACCCGAATGCTGCCCGGGTGAGAAACCTGGCAAAGAAAACGGTCTCGAC
  AATGAGTAGGCCACCCATCACTACTAACTACAGATGACTTGCCATTTCATTTACAAAGAT
  GTCTTCTGCTGCTGAAAATGGAGAGGCAGCACCTGGAAAACAAAATGAAGAAAAAACCTA
  TAAAAAGACTGCATCATCTGCTATTAAAGGTGCTATTCAGCTGGGAATAGGATACACAGT
  GGGTAATCTCACTTCCAAGCCAGAACGAGATGTTCTTATGCAAGACTTTTATGTGGTGGA
  AAGTGTGTTCCTACCCAGCGAAGGGAGCAATCTGACCCCAGCACATCACTACCCAGACTT
  TAGATTTAAGACATACGCTCCATTAGCATTCCGATATTTCAGAGAACTTTTTGGTATCAA
  GCCTGATGATTACTTGTATTCCATCTGCAGTGAACCTCTAATAGAACTGTCTAACCCTGG
  AGCCAGTGGATCCTTGTTTTTTGTGACCAGTGATGATGAATTTATCATCAAAACAGTTCA
  GCACANAGAAGCTGAGTTTCTTCAGAAGCTACTGCCAGGCTATTACATGAATTTTTAACC
  AGATCCNANGACTCTTTTGCCAAAATTTACNGACTGTATTGTATGCAATCAGGAGGCATT
  AATATCAGGGATGTGGGTGATGAACCANNCGTTTGNCCACGCTCATGAGAATGCACTTTA
  CATATGACTTTGAAGGGCTCACGTATAAGCGAAGAGCATCCCGTAAAGAGAGAGAGAAAT
  CCACCCCCATTTTAGGACTTAGATTCCTGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003558 unedited
  GTACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAGAACAAAATATT
  TTATTTCTCTCACAAAACTATAGTTTACAGTAGTATTTACTAAATAAATTATATAATTTC
  TCTTTTACAAGCAATAAACTAGATCACAAATATTTCAAAGACAATGTATGTACACGACAT
  AAGGCCTTTTATAAATAGCTTTGCTTTTCTCATTTCACTAGTAAACAGTGAAAAAATCTT
  TATAGAAGCTAGTTTTCTATAAAGTGAAGGCAGACCTAGTATATTGATCTTTAAATGAAA
  CCAATTTAAACTTTCAATGACATGAACTATAATTTTGCAAAAGAATTTATACTTAATTTA
  CCATGTGTGTGGATGGATTTTTAAAAATATATAAGCAGATTACATATGTTGCTTATGTAT
  AATCACTGGATGCATGCACAATGAAATAATGCAAAAGTCCATAGGAAAATAGCACTTCCA
  ACTCCAAAAAGGTGATTGGCAATGGTACAGAAAGCAGCTTAAATATAGTTCATGCAGTTC
  TTCACAGTTCAGCAAGCAACAGCAAATGATAGAATAGGGTAGTATTTTTCACATCCCATC
  TGCTGATCAGCCTGAAGTTGACATCTTGAAAACTCTTAAGTCAGGTTGATGATTTCTCTG
  TGTGTAGTGGGGCTCACCCAAGTTGCTGTGGATAATTTTTGGTGCGGAGAAACTTCTCTG
  CCATAACTGTGCTCACGTCCCATCCATGGGCTTAAGCGACCCCCATTTTCACTTATAAAT
  AGACGTTCAGCACCACAACATTGGCTTCCCGCACTTACCAAGAGTTGGGCTCACCGGTCA
  CATTAATGGTCCCCTCTTCCAAGGAAAAGTTGAACCGGAAGCAACCCTTTGTTGTTGCAA
  AAACGGACGCGCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003558
• Insert Size: 2570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003558.1, NP_003549.1
• RefSeq Size: 2764 bp
• RefSeq ORF: 1623 bp
• Locus ID: 8395
• UniProt ID: O14986
• Cytogenetics: 9q21.11
• Domains: PIP5K
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
• Gene Summary: Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of PLD2. Together with PIP5K1A is required after stimulation of G-protein coupled receptors for stable platelet adhesion (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2).