GLIS2 (NM_032575) Human Tagged ORF Clone

CAT#: RC223616

GLIS2 (Myc-DDK-tagged)-Human GLIS family zinc finger 2 (GLIS2)

Product Images

Circular map for RC223616

• 

  Western blot validation of overexpression lysate (Cat# LY410031) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223616 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GLIS2
• Synonyms: NKL; NPHP7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223616 representing NM_032575
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACTCCCTGGACGAGCCGCTCGACCTGAAGCTGAGTATCACCAAGCTCCGGGCGGCAAGAGAGAAGC
  GGGAGAGGACGCTGGGTGTGGTCCGGCCCCGTGCTCTGCACAGGGAGCTGGGCCTGGTGGATGACAGCCC
  CACACCTGGCTCTCCAGGCTCCCCGCCCTCAGGCTTCCTGCTGAACTCCAAGTTCCCCGAGAAGGTGGAG
  GGACGCTTTTCAGCAGCCCCTCTCGTGGACCTCAGCCTGTCACCACCATCTGGGCTGGACTCCCCCAATG
  GCAGCAGCTCGCTGTCCCCCGAGCGCCAGGGCAACGGGGACCTGCCTCCAGTGCCCAGTGCCTCGGACTT
  CCAGCCACTGCGCTATTTGGATGGTGTCCCCAGCTCCTTCCAGTTCTTCCTGCCCCTCGGCTCCGGGGGG
  GCCCTGCACCTGCCTGCCTCCTCCTTCCTTACCCCTCCCAAGGACAAGTGCCTCTCGCCAGACCTGCCCC
  TGCCCAAGCAGCTGGTGTGTCGCTGGGCCAAGTGTAACCAGCTCTTTGAGCTCCTGCAAGACCTGGTGGA
  CCATGTCAACGATTACCATGTCAAGCCCGAGAAGGATGCGGGGTACTGCTGCCACTGGGAGGGCTGCGCC
  CGCCATGGCCGAGGTTTCAACGCCAGGTACAAGATGCTCATCCACATCCGCACACACACCAACGAGAAGC
  CACACCGCTGTCCGACCTGCAGCAAGAGCTTCTCCCGCCTGGAGAACCTGAAGATCCACAACCGGTCGCA
  CACAGGTGAGAAGCCCTACGTCTGCCCCTACGAGGGCTGCAACAAGCGCTATTCCAACTCCAGTGACCGC
  TTTAAGCACACGCGCACCCACTATGTGGACAAGCCCTACTACTGCAAGATGCCCGGCTGCCACAAGCGCT
  ACACGGACCCCAGCTCACTGCGCAAGCACATCAAGGCCCATGGCCACTTTGTGTCCCACGAGCAGCAAGA
  GCTCCTGCAGCTGCGCCCACCCCCCAAGCCGCCACTGCCCGCCCCCGACGGCGGCCCCTATGTCAGTGGG
  GCCCAGATCATCATCCCCAACCCAGCTGCCCTCTTTGGAGGCCCTGGCCTGCCCGGCTTACCCCTACCCC
  TGGCCCCCGGCCCCCTTGACCTCAGTGCCCTGGCCTGTGGCAACGGTGGGGGCAGTGGGGGTGGGGGGGG
  CATGGGCCCTGGGCTGCCAGGCCCCGTCCTGCCTCTCAATCTGGCCAAGAACCCGCTGCTGCCCTCGCCC
  TTTGGGGCTGGCGGACTGGGCTTGCCTGTGGTCTCCCTCCTTGCTGGCGCAGCTGGTGGCAAGGCCGAGG
  GGGAGAAGGGGCGTGGGTCGGTGCCCACCAGGGCCCTGGGCATGGAGGGCCACAAGACGCCCCTTGAAAG
  GACGGAGAGCAGCTGCTCCCGGCCAAGCCCCGATGGACTCCCCCTGCTGCCAGGCACCGTGCTGGACCTG
  TCCACGGGCGTCAACTCAGCTGCCAGCAGCCCAGAGGCGTTGGCCCCTGGCTGGGTGGTCATCCCGCCGG
  GCTCGGTGCTGCTCAAACCGGCTGTGGTGAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223616 representing NM_032575
  Red=Cloning site Green=Tags(s)
  
  MHSLDEPLDLKLSITKLRAAREKRERTLGVVRPRALHRELGLVDDSPTPGSPGSPPSGFLLNSKFPEKVE
  GRFSAAPLVDLSLSPPSGLDSPNGSSSLSPERQGNGDLPPVPSASDFQPLRYLDGVPSSFQFFLPLGSGG
  ALHLPASSFLTPPKDKCLSPDLPLPKQLVCRWAKCNQLFELLQDLVDHVNDYHVKPEKDAGYCCHWEGCA
  RHGRGFNARYKMLIHIRTHTNEKPHRCPTCSKSFSRLENLKIHNRSHTGEKPYVCPYEGCNKRYSNSSDR
  FKHTRTHYVDKPYYCKMPGCHKRYTDPSSLRKHIKAHGHFVSHEQQELLQLRPPPKPPLPAPDGGPYVSG
  AQIIIPNPAALFGGPGLPGLPLPLAPGPLDLSALACGNGGGSGGGGGMGPGLPGPVLPLNLAKNPLLPSP
  FGAGGLGLPVVSLLAGAAGGKAEGEKGRGSVPTRALGMEGHKTPLERTESSCSRPSPDGLPLLPGTVLDL
  STGVNSAASSPEALAPGWVVIPPGSVLLKPAVVN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032575
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032575.3
• RefSeq Size: 3705 bp
• RefSeq ORF: 1575 bp
• Locus ID: 84662
• UniProt ID: Q9BZE0
• Cytogenetics: 16p13.3
• Domains: zf-C2H2
• Protein Families: ES Cell Differentiation/IPS
• MW: 55.5 kDa
• Gene Summary: This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear transcription factor with five C2H2-type zinc finger domains. The protein encoded by this gene is widely expressed at low levels in the neural tube and peripheral nervous system and likely promotes neuronal differentiation. It is abundantly expressed in the kidney and may have a role in the regulation of kidney morphogenesis. p120 regulates the expression level of this protein and induces the cleavage of this protein's C-terminal zinc finger domain. This protein also promotes the nuclear translocation of p120. Mutations in this gene cause nephronophthisis (NPHP), an autosomal recessive kidney disease characterized by tubular basement membrane disruption, interstitial lymphohistiocytic cell infiltration, and development of cysts at the corticomedullary border of the kidneys.[provided by RefSeq, Jan 2010]