RASGRF2 (NM_006909) Human Tagged ORF Clone

CAT#: RC223580

RASGRF2 (Myc-DDK-tagged)-Human Ras protein-specific guanine nucleotide-releasing factor 2 (RASGRF2)

Product Images

Circular map for RC223580

• 

  Western blot validation of overexpression lysate (Cat# LY416330) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223580 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RASGRF2 protein (Cat# TP323580). The protein was produced from HEK293T cells transfected with RASGRF2 cDNA clone (Cat# RC223580) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RASGRF2
• Synonyms: GRF2; RAS-GRF2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223580 representing NM_006909
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAGAGCGTGCGCTACAACGAGGGGCACGCCCTGTACCTGGCCTTTCTGGCGCGCAAGGAGGGCA
  CCAAGCGCGGCTTCCTGAGTAAGAAGACGGCCGAGGCGAGCCGCTGGCACGAGAAGTGGTTCGCCCTCTA
  CCAGAATGTGCTCTTCTACTTCGAGGGCGAGCAGAGCTGCCGCCCGGCGGGCATGTACCTCCTGGAGGGC
  TGCAGCTGCGAACGAACGCCCGCGCCACCCAGGGCCGGCGCCGGGCAGGGAGGCGTCCGAGACGCGCTGG
  ACAAGCAGTATTACTTTACTGTTCTTTTTGGCCATGAAGGTCAGAAGCCACTGGAGCTGCGCTGTGAGGA
  GGAGCAGGATGGTAAAGAGTGGATGGAGGCCATTCACCAAGCCAGTTATGCAGACATTTTGATTGAGAGG
  GAAGTATTAATGCAGAAGTACATTCATCTAGTTCAGATCGTAGAGACAGAAAAAATTGCAGCTAACCAAC
  TCCGACATCAACTTGAAGATCAAGACACAGAAATCGAAAGGCTTAAATCAGAGATTATTGCTCTTAATAA
  AACCAAAGAACGAATGCGACCTTACCAAAGCAACCAAGAAGACGAAGATCCAGACATCAAGAAGATTAAA
  AAGGTTCAGAGCTTCATGCGAGGATGGTTGTGCAGAAGGAAATGGAAGACCATCGTGCAGGATTACATTT
  GTTCTCCTCATGCTGAAAGTATGAGGAAGAGAAACCAGATTGTGTTCACCATGGTGGAGGCAGAGTCAGA
  GTACGTTCACCAGCTCTACATCCTGGTCAATGGCTTTCTCCGGCCCCTGCGTATGGCCGCCAGCTCCAAG
  AAGCCCCCCATCAGCCACGACGACGTCAGCAGTATTTTTCTTAACAGTGAAACAATCATGTTTCTTCATG
  AAATATTTCATCAAGGACTAAAGGCAAGGATAGCAAACTGGCCCACTTTAATTTTAGCTGATCTGTTTGA
  TATTTTGCTCCCCATGCTGAACATTTATCAAGAATTTGTGCGTAATCACCAGTACAGCCTGCAAGTTCTC
  GCCAATTGTAAGCAAAACAGAGATTTTGACAAACTCTTAAAACAGTATGAAGCCAATCCAGCCTGTGAGG
  GGAGGATGCTGGAGACATTCTTGACCTATCCCATGTTTCAGATCCCCAGATATATCATCACACTCCATGA
  GCTCCTTGCTCACACACCCCATGAGCATGTGGAAAGGAAAAGCCTGGAGTTTGCCAAATCAAAGCTAGAG
  GAACTATCCAGAGTAATGCACGATGAAGTCAGCGACACTGAAAACATAAGGAAAAACCTTGCCATCGAAA
  GAATGATCGTGGAGGGCTGTGACATCTTGCTGGACACCAGCCAAACGTTCATCCGCCAAGGTTCTCTTAT
  TCAAGTACCTTCCGTTGAGAGGGGGAAACTTAGTAAAGTTCGCCTGGGTTCGTTGTCTTTGAAAAAGGAA
  GGAGAGAGACAATGCTTCTTATTTACAAAACACTTTTTAATATGTACAAGAAGTTCAGGAGGGAAGCTTC
  ATCTGCTCAAGACAGGTGGGGTTCTGTCTCTAATAGACTGCACATTGATTGAGGAGCCAGATGCAAGCGA
  TGATGACTCTAAAGGTTCTGGGCAAGTGTTTGGGCACCTGGATTTTAAAATAGTGGTGGAGCCTCCTGAC
  GCTGCCGCCTTCACTGTTGTCTTGTTAGCACCCTCACGCCAGGAGAAAGCTGCCTGGATGAGTGACATCA
  GTCAGTGTGTGGACAATATACGATGTAATGGTTTAATGACTATAGTGTTTGAAGAGAATTCCAAAGTCAC
  TGTGCCACATATGATTAAGTCTGATGCCCGTCTTCATAAAGACGACACTGACATTTGCTTCAGTAAAACA
  CTCAACTCCTGCAAAGTGCCCCAGATCCGTTATGCCAGCGTGGAGCGCCTCTTGGAACGACTGACAGACT
  TGCGGTTTCTTAGTATTGATTTCCTCAACACCTTTCTGCACACCTATCGTATTTTCACTACTGCCGCTGT
  GGTGCTGGGGAAACTCTCCGACATATACAAGAGGCCTTTCACCTCCATCCCTGTCAGGTCATTGGAATTG
  TTTTTTGCTACCAGCCAGAACAACAGAGGTGAACATTTGGTGGATGGCAAATCCCCACGTCTGTGTCGCA
  AATTCTCTTCCCCGCCACCACTGGCTGTGTCCAGAACATCTTCCCCAGTGAGGGCCAGAAAGCTGTCTTT
  GACTTCTCCCTTGAACTCAAAGATAGGAGCATTGGACCTGACAACTTCCAGCAGTCCCACCACCACCACC
  CAGAGTCCCGCTGCGTCTCCACCACCACACACTGGTCAGATACCACTGGATCTCAGCAGAGGCCTCTCTT
  CTCCAGAGCAAAGCCCGGGAACGGTAGAAGAGAATGTCGATAACCCACGCGTGGATCTGTGTAACAAGCT
  AAAACGAAGTATTCAAAAAGCAGTCCTAGAGTCTGCACCAGCGGACCGAGCAGGAGTGGAAAGCTCCCCT
  GCAGCGGACACCACAGAACTTTCACCTTGCAGATCCCCCTCAACTCCTCGGCACCTCCGCTATCGACAGC
  CTGGAGGACAGACGGCGGACAATGCCCACTGCTCTGTTTCACCGGCTTCTGCTTTTGCAATAGCCACAGC
  TGCAGCAGGACATGGGAGTCCACCAGGCTTTAACAACACCGAGAGAACATGTGATAAAGAGTTTATTATA
  CGGAGAACGGCTACCAATCGAGTTCTGAACGTCCTCCGTCACTGGGTCTCAAAGCACGCACAGGATTTCG
  AACTCAACAATGAACTAAAGATGAATGTCCTAAATTTGCTAGAAGAAGTTTTGCGAGACCCAGACCTTCT
  TCCCCAAGAAAGGAAAGCCGCCGCGAATATCCTCAGGGCCCTTTCACAAGATGACCAAGATGACATCCAC
  CTAAAATTAGAGGATATAATTCAAATGACTGACTGCATGAAGGCCGAATGCTTTGAGTCCTTGTCGGCCA
  TGGAGCTGGCAGAACAGATCACCCTCCTGGACCATGTCATTTTCAGAAGCATTCCCTACGAGGAGTTTCT
  TGGGCAGGGGTGGATGAAGCTGGATAAAAACGAAAGAACTCCTTACATTATGAAAACCAGCCAACACTTC
  AATGACATGAGTAACCTGGTGGCCTCCCAGATAATGAACTATGCTGATGTCAGCTCCCGTGCCAACGCCA
  TCGAGAAATGGGTGGCAGTGGCGGACATCTGCCGATGCCTGCACAACTACAACGGCGTGCTGGAGATCAC
  CTCGGCCTTAAACAGAAGTGCCATCTACAGGCTGAAGAAAACCTGGGCCAAGGTCTCTAAGCAGACAAAA
  GCTCTAATGGACAAACTTCAAAAGACTGTTTCCTCTGAAGGAAGATTTAAAAATCTTAGAGAAACCCTTA
  AAAATTGTAACCCTCCTGCAGTTCCTTATCTTGGGATGTACTTGACAGACCTGGCATTTATTGAAGAAGG
  AACACCAAACTTTACTGAGGAAGGCCTTGTCAATTTCTCCAAAATGAGAATGATATCACACATCATCAGA
  GAGATACGCCAGTTCCAGCAGACTTCCTACAGAATAGATCATCAGCCAAAGGTCGCACAGTACTTGCTTG
  ACAAAGACCTTATCATAGATGAAGATACGCTATATGAGCTGTCACTAAAAATTGAACCTCGACTCCCTGC
  T
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223580 representing NM_006909
  Red=Cloning site Green=Tags(s)
  
  MQKSVRYNEGHALYLAFLARKEGTKRGFLSKKTAEASRWHEKWFALYQNVLFYFEGEQSCRPAGMYLLEG
  CSCERTPAPPRAGAGQGGVRDALDKQYYFTVLFGHEGQKPLELRCEEEQDGKEWMEAIHQASYADILIER
  EVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIK
  KVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSK
  KPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVL
  ANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLE
  ELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKE
  GERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIDCTLIEEPDASDDDSKGSGQVFGHLDFKIVVEPPD
  AAAFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKT
  LNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLEL
  FFATSQNNRGEHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSKIGALDLTTSSSPTTTT
  QSPAASPPPHTGQIPLDLSRGLSSPEQSPGTVEENVDNPRVDLCNKLKRSIQKAVLESAPADRAGVESSP
  AADTTELSPCRSPSTPRHLRYRQPGGQTADNAHCSVSPASAFAIATAAAGHGSPPGFNNTERTCDKEFII
  RRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKAAANILRALSQDDQDDIH
  LKLEDIIQMTDCMKAECFESLSAMELAEQITLLDHVIFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHF
  NDMSNLVASQIMNYADVSSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTK
  ALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIR
  EIRQFQQTSYRIDHQPKVAQYLLDKDLIIDEDTLYELSLKIEPRLPA
  
  TRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006909
• ORF Size: 3711 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006909.3
• RefSeq Size: 4078 bp
• RefSeq ORF: 3714 bp
• Locus ID: 5924
• UniProt ID: O14827
• Cytogenetics: 5q14.1
• Protein Pathways: MAPK signaling pathway
• MW: 140.6 kDa
• Gene Summary: RAS GTPases cycle between an inactive GDP-bound state and an active GTP-bound state. This gene encodes a calcium-regulated nucleotide exchange factor activating both RAS and RAS-related protein, RAC1, through the exchange of bound GDP for GTP, thereby, coordinating the signaling of distinct mitogen-activated protein kinase pathways. [provided by RefSeq, Oct 2011]