PPP1R14B (NM_138689) Human Tagged ORF Clone
CAT#: RC223367
PPP1R14B (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 14B (PPP1R14B)
ORF Plasmid: tGFP
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_138689" in other vectors (4)
USD 198.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PPP1R14B |
Synonyms | PHI-1; PLCB3N; PNG; SOM172 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223367 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACAGCGGCACCGCGGGGGGCGCGGCGTTGGCGGCCCCGGCCCCCGGGCCGGGCAGTGGCGGCC CAGGACCACGCGTCTACTTTCAGAGCCCCCCCGGGGCCGCAGGAGAGGGCCCGGGCGGGGCGGACGATGA GGGCCCAGTGAGGCGCCAAGGGAAGGTCACCGTCAAGTATGACCGCAAGGAGCTACGGAAGCGCCTCAAC CTAGAGGAGTGGATCCTGGAGCAGCTCACGCGCCTCTACGACTGCCAGGAAGAGGAGATCCCAGAACTGG AGATTGACGTGGATGAGCTCCTGGACATGGAGAGTGACGATGCCCGGGCTGCCAGGGTCAAGGAGCTGCT GGTTGACTGTTACAAACCCACAGAGGCCTTCATTTCTGGCCTGCTGGACAAGATCCGGGGCATGCAGAAG CTGAGCACACCCCAGAAGAAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC223367 protein sequence
Red=Cloning site Green=Tags(s) MADSGTAGGAALAAPAPGPGSGGPGPRVYFQSPPGAAGEGPGGADDEGPVRRQGKVTVKYDRKELRKRLN LEEWILEQLTRLYDCQEEEIPELEIDVDELLDMESDDARAARVKELLVDCYKPTEAFISGLLDKIRGMQK LSTPQKK SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_138689 |
ORF Size | 441 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138689.3 |
RefSeq Size | 1038 bp |
RefSeq ORF | 444 bp |
Locus ID | 26472 |
UniProt ID | Q96C90 |
Cytogenetics | 11q13.1 |
Domains | PP1_inhibitor |
MW | 15.9 kDa |
Gene Summary | Inhibitor of PPP1CA. Has over 50-fold higher inhibitory activity when phosphorylated (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223367L3 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 14B (PPP1R14B), Myc-DDK-tagged |
USD 450.00 |
|
RC223367L4 | Lenti ORF clone of Human protein phosphatase 1, regulatory (inhibitor) subunit 14B (PPP1R14B), mGFP tagged |
USD 450.00 |
|
RG223367 | PPP1R14B (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 14B (PPP1R14B) |
USD 350.00 |
|
SC120667 | PPP1R14B (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 14B (PPP1R14B) |
USD 150.00 |
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