ASTL (NM_001002036) Human Tagged ORF Clone

CAT#: RC223331

ASTL (Myc-DDK-tagged)-Human astacin-like metallo-endopeptidase (M12 family) (ASTL)

Product Images

Circular map for RC223331

• 

  Western blot validation of overexpression lysate (Cat# LY424175) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223331 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASTL
• Synonyms: SAS1B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223331 representing NM_001002036
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGTGTAGGGGGTCTCTGGCCTTGGGTGCTGGGTCTGCTCTCCTTGCCAGGTGTGATCCTAGGAG
  CGCCCCTGGCCTCCAGCTGCGCAGGAGCCTGTGGTACCAGCTTCCCAGATGGCCTCACCCCTGAGGGAAC
  CCAGGCCTCCGGGGACAAGGACATTCCTGCAATTAACCAAGGGCTCATCCTGGAAGAAACCCCAGAGAGC
  AGCTTCCTCATCGAGGGGGACATCATCCGGCCGAGTCCCTTCCGACTGCTGTCAGCAACCAGCAACAAAT
  GGCCCATGGGTGGTAGTGGTGTCGTGGAGGTCCCCTTCCTGCTCTCCAGCAAGTACGATGAGCCCAGCCG
  CCAGGTCATCCTGGAGGCTCTTGCGGAGTTTGAACGTTCCACGTGCATCAGGTTTGTCACCTATCAGGAC
  CAGAGAGACTTCATTTCCATCATCCCCATGTATGGGTGCTTCTCGAGTGTGGGGCGCAGTGGAGGGATGC
  AGGTGGTCTCCCTGGCGCCCACGTGTCTCCAGAAGGGCCGGGGCATTGTCCTTCATGAGCTCATGCATGT
  GCTGGGCTTCTGGCACGAGCACACGCGGGCCGACCGGGACCGCTATATCCGTGTCAACTGGAACGAGATC
  CTGCCAGGCTTTGAAATCAACTTCATCAAGTCTCGGAGCAGCAACATGCTGACGCCCTATGACTACTCCT
  CTGTGATGCACTATGGGAGGCTTGCCTTCAGCCGGCGTGGGCTGCCCACCATCACACCACTTTGGGCCCC
  CAGTGTCCACATCGGCCAGCGATGGAACCTGAGTGCCTCGGACATCACCCGGGTCCTCAAACTCTACGGC
  TGCAGCCCAAGTGGCCCCAGGCCCCGTGGGAGAGGGTCCCATGCCCACAGCACTGGTAGGAGCCCCGCTC
  CGGCCTCCCTATCTCTGCAGCGGCTTTTGGAGGCACTGTCGGCGGAATCCAGGAGCCCCGACCCCAGTGG
  TTCCAGTGCGGGAGGCCAGCCCGTTCCTGCAGGGCCTGGGGAGAGCCCACATGGGTGGGAGTCCCCTGCC
  CTGAAAAAGCTCAGTGCAGAGGCCTCGGCAAGGCAGCCTCAGACCCTGGCTTCCTCCCCAAGATCAAGGC
  CTGGAGCAGGTGCCCCCGGTGTTGCTCAGGAGCAGTCCTGGCTGGCCGGAGTGTCCACCAAGCCCACAGT
  CCCATCTTCAGAAGCAGGAATCCAGCCAGTCCCTGTCCAGGGAAGCCCAGCTCTGCCAGGGGGCTGTGTA
  CCTAGAAATCATTTCAAGGGGATGTCCGAAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223331 representing NM_001002036
  Red=Cloning site Green=Tags(s)
  
  MEGVGGLWPWVLGLLSLPGVILGAPLASSCAGACGTSFPDGLTPEGTQASGDKDIPAINQGLILEETPES
  SFLIEGDIIRPSPFRLLSATSNKWPMGGSGVVEVPFLLSSKYDEPSRQVILEALAEFERSTCIRFVTYQD
  QRDFISIIPMYGCFSSVGRSGGMQVVSLAPTCLQKGRGIVLHELMHVLGFWHEHTRADRDRYIRVNWNEI
  LPGFEINFIKSRSSNMLTPYDYSSVMHYGRLAFSRRGLPTITPLWAPSVHIGQRWNLSASDITRVLKLYG
  CSPSGPRPRGRGSHAHSTGRSPAPASLSLQRLLEALSAESRSPDPSGSSAGGQPVPAGPGESPHGWESPA
  LKKLSAEASARQPQTLASSPRSRPGAGAPGVAQEQSWLAGVSTKPTVPSSEAGIQPVPVQGSPALPGGCV
  PRNHFKGMSED
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002036
• ORF Size: 1293 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002036.3
• RefSeq Size: 1296 bp
• RefSeq ORF: 1296 bp
• Locus ID: 431705
• UniProt ID: Q6HA08
• Cytogenetics: 2q11.2
• Protein Families: Transmembrane
• MW: 45.8 kDa
• Gene Summary: Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding (By similarity).[UniProtKB/Swiss-Prot Function]