MAP3K15 (NM_001001671) Human Tagged ORF Clone

CAT#: RC223307

MAP3K15 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 15 (MAP3K15)

Product Images

Circular map for RC223307

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAP3K15 (Cat# RC223307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP3K15 (Cat# TA808730)(1:2000). Positive lysates LY400363 (100ug) and LC400363 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY400363) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223307 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified MAP3K15 protein (Cat# TP323307). The protein was produced from HEK293T cells transfected with MAP3K15 cDNA clone (Cat# RC223307) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAP3K15
• Synonyms: ASK3; bA723P2.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223307 representing NM_001001671
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATGTCTCACCCACAGAAATGAAACAGATGCACGAATGGAATTTTACAGCCTCTTCCATAAAGGGA
  ATAAGGCTGGAGTACAGTGGCACGATCTCGGCTCACTGCAACCTCTGCCTCCCAGGTTCAAGCGATTCTC
  CTGCCTCAGCCTCCAGAGTAGCTGGGACTACAGCCTATCAAAGTTTGATGAAAGGTGTTGTTTTCTTTAT
  GTCCATGATAATTCTGATGACTTTCAAATCTACTTTTCCACCGAAGAGCAGTGCAGTAGATTTTTCTCTT
  TGGTCAAAGAGATGATAACCAATACAGCAGGCAGTACGGTGGAGCTGGAGGGAGAGACCGATGGAGACAC
  CTTGGAGTATGAGTATGACCATGATGCAAATGGTGAGAGAGTTGTCTTGGGGAAAGGCACGTATGGGATT
  GTGTATGCTGGCCGAGATCTGAGCAATCAAGTGCGAATAGCCATCAAAGAAATCCCGGAGAGAGATAGCA
  GGTATTCTCAGCCTCTGCACGAGGAGATAGCCCTGCACAAGTACCTTAAGCACCGCAATATCGTTCAGTA
  CCTGGGCTCTGTTTCAGAGAACGGCTACATTAAGATATTTATGGAGCAGGTGCCTGGAGGAAGCCTTTCT
  GCTCTTCTGCGATCCAAATGGGGGCCGATGAAGGAACCGACAATCAAGTTTTACACCAAACAGATCCTGG
  AGGGCCTTAAGTATCTTCATGAAAACCAGATCGTGCACAGAGACATAAAGGGCGATAATGTTCTGGTGAA
  CACCTACAGCGGAGTGGTGAAAATCTCCGATTTTGGAACCTCGAAACGTCTTGCGGGTGTGAACCCCTGC
  ACAGAGACTTTTACTGGCACCCTGCAGTACATGGCACCTGAGATAATTGACCAAGGGCCTCGCGGATATG
  GTGCCCCAGCCGATATCTGGTCCCTGGGCTGCACCATCATTGAGATGGCCACCAGCAAGCCTCCGTTCCA
  TGAGCTTGGTGAGCCGCAGGCAGCCATGTTCAAAGTGGGCATGTTTAAGATCCACCCTGAGATTCCAGAA
  GCCCTTTCAGCTGAAGCCCGAGCCTTCATTTTATCCTGTTTCGAGCCTGACCCCCACAAACGTGCCACCA
  CTGCTGAGCTACTGAGAGAGGGTTTCTTAAGGCAGGTGAACAAGGGCAAGAAGAACCGAATTGCCTTCAA
  GCCCTCAGAAGGTCCCCGCGGTGTCGTCCTGGCCCTGCCCACACAGGGAGAGCCCATGGCCACCAGCAGC
  AGCGAGCACGGCTCTGTCTCCCCAGACTCCGACGCCCAGCCTGACGCACTCTTTGAGAGGACCCGGGCGC
  CCAGGCACCACCTTGGCCACCTCCTCAGTGTTCCAGACGAGAGCTCAGCCTTGGAAGACCGGGGCTTGGC
  CTCGTCCCCGGAGGACAGGGACCAGGGCCTCTTCCTGCTACGCAAGGACAGTGAGCGCCGTGCCATCCTG
  TACAAAATCCTCTGGGAGGAGCAGAACCAGGTGGCTTCCAACCTGCAGGAGTGTGTGGCCCAGAGTTCCG
  AAGAGTTGCATCTCTCAGTTGGACACATCAAGCAAATCATTGGGATCCTGAGGGACTTCATCCGCTCCCC
  AGAGCACCGGGTGATGGCGACCACAATATCAAAGCTCAAGGTGGACCTGGACTTTGACAGCTCGTCCATC
  AGTCAGATTCACCTGGTGCTGTTCGGATTTCAGGATGCCGTAAATAAAATTTTGAGGAACCACTTAATTA
  GGCCCCACTGGATGTTCGCGATGGACAACATCATCCGCCGAGCGGTGCAGGCCGCGGTCACCATTCTCAT
  CCCAGAGCTCCGAGCCCACTTTGAGCCTACCTGTGAGACTGAAGGGGTAGATAAGGACATGGATGAAGCG
  GAAGAGGGCTATCCCCCAGCCACCGGACCTGGCCAGGAGGCCCAGCCCCACCAGCAGCACCTGAGCCTCC
  AGCTGGGTGAGCTCAGACAGGAGACCAACAGACTTTTGGAACACCTAGTTGAAAAAGAGAGAGAGTACCA
  GAATCTTCTGCGGCAAACTCTAGAACAGAAAACTCAAGAATTGTATCACCTTCAGTTAAAATTAAAATCG
  AATTGTATTACAGAGAACCCAGCAGGCCCCTACGGGCAGAGAACAGATAAAGAGCTTATAGACTGGTTGC
  GGCTGCAAGGAGCTGATGCAAAGACAATTGAAAAGATTGTTGAAGAGGGTTATACACTTTCGGATATTCT
  TAATGAGATCACTAAGGAAGATCTAAGATACCTTCGACTACGGGGTGGTCTCCTCTGCAGACTCTGGAGT
  GCGGTCTCCCAGTACAGAAGGGCTCAGGAGGCCTCAGAAACCAAAGACAAGGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223307 representing NM_001001671
  Red=Cloning site Green=Tags(s)
  
  MACLTHRNETDARMEFYSLFHKGNKAGVQWHDLGSLQPLPPRFKRFSCLSLQSSWDYSLSKFDERCCFLY
  VHDNSDDFQIYFSTEEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGI
  VYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLS
  ALLRSKWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLAGVNPC
  TETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTIIEMATSKPPFHELGEPQAAMFKVGMFKIHPEIPE
  ALSAEARAFILSCFEPDPHKRATTAELLREGFLRQVNKGKKNRIAFKPSEGPRGVVLALPTQGEPMATSS
  SEHGSVSPDSDAQPDALFERTRAPRHHLGHLLSVPDESSALEDRGLASSPEDRDQGLFLLRKDSERRAIL
  YKILWEEQNQVASNLQECVAQSSEELHLSVGHIKQIIGILRDFIRSPEHRVMATTISKLKVDLDFDSSSI
  SQIHLVLFGFQDAVNKILRNHLIRPHWMFAMDNIIRRAVQAAVTILIPELRAHFEPTCETEGVDKDMDEA
  EEGYPPATGPGQEAQPHQQHLSLQLGELRQETNRLLEHLVEKEREYQNLLRQTLEQKTQELYHLQLKLKS
  NCITENPAGPYGQRTDKELIDWLRLQGADAKTIEKIVEEGYTLSDILNEITKEDLRYLRLRGGLLCRLWS
  AVSQYRRAQEASETKDKA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001671
• ORF Size: 2364 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001671.2
• RefSeq Size: 4633 bp
• RefSeq ORF: 3942 bp
• Locus ID: 389840
• UniProt ID: Q6ZN16
• Cytogenetics: Xp22.12
• Protein Families: Druggable Genome, Protein Kinase
• MW: 89.4 kDa
• Gene Summary: The protein encoded by this gene is a member of the mitogen-activated protein kinase (MAPK) family. These family members function in a protein kinase signal transduction cascade, where an activated MAPK kinase kinase (MAP3K) phosphorylates and activates a specific MAPK kinase (MAP2K), which then activates a specific MAPK. This MAP3K protein plays an essential role in apoptotic cell death triggered by cellular stresses. [provided by RefSeq, Jul 2010]