CSPP1 (NM_024790) Human Tagged ORF Clone

CAT#: RC223163

CSPP1 (Myc-DDK-tagged)-Human centrosome and spindle pole associated protein 1 (CSPP1)

Product Images

Circular map for RC223163

• 

  Western blot validation of overexpression lysate (Cat# LY411051) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223163 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CSPP1
• Synonyms: CSPP; CSPP-L; JBTS21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223163 representing NM_024790
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTTCCCGCTCCAGGTGGCCGCTGTAACCTCTTCGGTCCGCGACGATCCTCTAGAGCACTGTGTGT
  CTCCCCGGACGCGAGCCCGCTCCCCTGAAATCTGCAAAATGGCTGATAATTTGGATGAATTTATTGAAGA
  GCAAAAAGCCAGATTGGCCGAAGACAAAGCAGAGTTGGAAAGTGATCCACCTTACATGGAAATGAAGGGA
  AAGTTGTCAGCGAAGCTTTCTGAAAACAGTAAGATACTGATCTCTATGGCTAAGGAAAACATACCACCAA
  ATAGTCAACAGACCAGGGGTTCCTTAGGAATTGATTATGGATTAAGTTTACCACTTGGAGAAGACTATGA
  ACGGAAGAAACATAAATTAAAAGAAGAATTGCGGCAAGATTACAGACGTTATCTTACTCAGGAAAGGTTG
  AAACTTGAACGTAACAAAGAATACAATCAGTTTCTCAGGGGTAAGGAAGAATCCAGTGAAAAGTTCAGGC
  AGGTGGAAAAGAGTACTGAGCCCAAGAGTCAGAGAAATAAAAAACCTATTGGTCAAGTTAAGCCTGATCT
  AACTTCACAAATACAGACATCTTGTGAAAATTCAGAGGGTCCTAGAAAAGATGTCTTAACTCCTTCAGAG
  GCATATGAAGAACTTCTGAACCAAAGACGACTAGAGGAGGACAGATACCGACAACTAGATGATGAAATCG
  AATTAAGGAATAGAAGAATTATTAAAAAAGCAAATGAAGAAGTGGGCATTTCCAACCTAAAACATCAAAG
  GTTTGCAAGCAAGGCTGGCATTCCAGATAGAAGATTTCACAGATTTAATGAGGATCGTGTTTTTGATAGA
  CGGTATCATAGACCAGACCAAGATCCTGAAGTAAGTGAAGAAATGGATGAGAGGTTTAGATATGAAAGTG
  ATTTTGATAGAAGACTTTCGAGAGTGTATACAAATGACAGGATGCACAGGAACAAACGAGGGAATATGCC
  TCCTATGGAACATGATGGGGATGTTATAGAACAGTCAAACATAAGAATTTCATCTGCTGAAAATAAAAGT
  GCTCCAGACAATGAAACATCCAAATCTGCTAATCAAGATACCTGTAGTCCTTTTGCAGGGATGCTCTTTG
  GAGGTGAAGATCGAGAACTTATTCAGAGAAGGAAAGAGAAATACAGACTAGAACTGTTGGAACAAATGGC
  TGAGCAACAGAGGAACAAGAGACGAGAAAAAGATTTAGAACTCAGGGTTGCAGCGTCTGGAGCACAAGAC
  CCTGAGAAATCGCCTGATAGACTAAAGCAGTTTAGTGTGGCACCAAGACACTTTGAAGAGATGATACCAC
  CTGAAAGACCCAGAATAGCTTTCCAGACACCTCTCCCTCCTTTATCTGCCCCATCTGTCCCACCCATCCC
  ATCAGTTCATCCTGTTCCTTCTCAAAATGAAGATTTGCGCAGTGGACTCAGCAGCGCCCTTGGTGAAATG
  GTGTCTCCCAGGATTGCACCTCTGCCTCCACCTCCCCTACTACCACCTTTGGCTACTAACTATCGAACTC
  CTTATGATGATGCATACTATTTTTATGGGTCCAGGAATACTTTCGATCCCAGTCTTGCTTATTATGGTTC
  AGGAATGATGGGCGTACAGCCTGCAGCTTATGTTAGTGCTCCTGTCACCCACCAACTAGCACAACCTGTT
  GTGAATACGGTTGGACAGAATGAACTGAAGATTACAAGTGATCAAGTGATAAATTCAGGATTGATTTTTG
  AAGATAAACCGAAACCTTCCAAACAGTCACTTCAGTCTTACCAAGAGGCTTTGCAGCAGCAGATTCGGGA
  AAGAGAAGAAAGAAGGAAGAAAGAACGTGAAGAAAAAGAAGAATATGAAGCTAAATTAGAAGCTGAAATG
  AGAACATATAATCCCTGGGGAAAAGGTGGAGGTGGTGCTCCTCTCAGGGATGCAAAAGGAAATCTGATAA
  CTGATTTGAATAGGATGCACAGACAAAATATAGATGCCTACCATAACCCAGATGCAAGAACATATGAAGA
  TAAAAGGGCTGTTGTATCTCTAGACCCAAATTTAGCCACTTCAAATGCTGAGAACCTAGAAGATGCTGCA
  AATAAAAGCTCAGGTCATATGCAAACACAGAGCTCTCCTTTTGCTCGGGGAAATGTATTTGGTGAGCCTC
  CAACTGAACTTCAGATTAAACAGCAAGAATTATACAAGAATTTTCTTCGTTTCCAGATTGAGGAAAAGAA
  ACAAAGAGAGGAAGCAGAGCGAGAGAGACTGAGAATTGCAGAAGAAAAAGAAGAAAGACGGCTTGCAGAA
  CAGAGGGCACGAATTCAGCAGGAGTATGAAGAGGAACAGGAAAAGAAAAGAGAGAAAGAGGAGGAGCAAA
  GGCTAAAAAATGAAGAGCATATTCGGTTAGCTGAAGAAAGACAAAAAGAAGCAGAAAGAAAGAAGAAAGA
  AGAAGAAGAAAAATATAACCTGCAACTTCAGCACTACTGTGAAAGAGACAATTTGATTGGGGAAGAAACA
  AAGCACATGAGACAGCCTTCTCCTATAGTTCCTGCTCTTCAGAACAAAATTGCAAGCAAACTCCAAAGAC
  CTCCTTCAGTTGACAGCATCATACGTTCCTTTATTCATGAAAGTTCCATGTCCAGGGCACAGTCACCCCC
  GGTACCTGCCAGGAAAAATCAGCTCCGTGCAGAAGAGGAGAAAAAAAATGTAATTATGGAATTATCAGAA
  ATGAGAAAACAGCTTCGTAGTGAAGAGAGGCGTCTACAAGAGCGATTGCTACACATGGACAGTGATGATG
  AAATTCCTATCAGGAAAAAGGAAAGGAATCCCATGGATATATTTGATATGGCTAGACATCGGTTGCAAGC
  TCCTGTCAGAAGACAGTCCCCTAAGGGCTTAGACGCTGCCACTTTTCAGAATGTTCATGATTTTAATGAG
  CTGAAAGATAGAGATTCAGAAACACGAGTTGATCTGAAATTTATGTACCTGGATCCTCCAAGAGATCATC
  ACACCTTAGAGATTCAGCAGCAAGCCCTGCTAAGAGAGCAGCAGAAGAGGCTGAACAGAATAAAAATGCA
  GGAAGGTGCCAAAGTTGACTTAGATGCCATCCCAAGTGCTAAAGTACGAGAGCAAAGAATGCCCAGAGAT
  GACACTAGTGATTTCTTGAAAAACTCATTATTGGAATCTGATAGTGCTTTTATTGGGGCTTACGGTGAGA
  CATATCCTGCCATTGAAGATGACGTCCTCCCTCCACCATCACAGTTGCCCTCTGCACGGGAGCGCAGGAG
  GAACAAATGGAAAGGACTAGACATTGATAGCAGTCGTCCTAATGTAGCACCAGATGGTCTCTCTCTAAAA
  TCTATATCCAGTGTAAATGTTGATGAGCTTAGAGTGAGAAATGAGGAACGAATGCGAAGACTGAATGAAT
  TTCACAATAAACCTATTAATACAGATGATGAGAGTTCACTGGTTGACCCTGATGACATCATGAAACACAT
  AGGGGATGACGGATCAAACTCTGTAGCAACTGAGCCCTGGCTCCGCCCTGGCACTTCAGAAACGCTGAAA
  CGTTTCATGGCAGAGCAGCTGAACCAGGAGCAGCAGCAGATTCCTGGAAAACCAGGCACTTTCACTTGGC
  AGGGCCTGTCGACTGCACATGGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223163 representing NM_024790
  Red=Cloning site Green=Tags(s)
  
  MLFPLQVAAVTSSVRDDPLEHCVSPRTRARSPEICKMADNLDEFIEEQKARLAEDKAELESDPPYMEMKG
  KLSAKLSENSKILISMAKENIPPNSQQTRGSLGIDYGLSLPLGEDYERKKHKLKEELRQDYRRYLTQERL
  KLERNKEYNQFLRGKEESSEKFRQVEKSTEPKSQRNKKPIGQVKPDLTSQIQTSCENSEGPRKDVLTPSE
  AYEELLNQRRLEEDRYRQLDDEIELRNRRIIKKANEEVGISNLKHQRFASKAGIPDRRFHRFNEDRVFDR
  RYHRPDQDPEVSEEMDERFRYESDFDRRLSRVYTNDRMHRNKRGNMPPMEHDGDVIEQSNIRISSAENKS
  APDNETSKSANQDTCSPFAGMLFGGEDRELIQRRKEKYRLELLEQMAEQQRNKRREKDLELRVAASGAQD
  PEKSPDRLKQFSVAPRHFEEMIPPERPRIAFQTPLPPLSAPSVPPIPSVHPVPSQNEDLRSGLSSALGEM
  VSPRIAPLPPPPLLPPLATNYRTPYDDAYYFYGSRNTFDPSLAYYGSGMMGVQPAAYVSAPVTHQLAQPV
  VNTVGQNELKITSDQVINSGLIFEDKPKPSKQSLQSYQEALQQQIREREERRKKEREEKEEYEAKLEAEM
  RTYNPWGKGGGGAPLRDAKGNLITDLNRMHRQNIDAYHNPDARTYEDKRAVVSLDPNLATSNAENLEDAA
  NKSSGHMQTQSSPFARGNVFGEPPTELQIKQQELYKNFLRFQIEEKKQREEAERERLRIAEEKEERRLAE
  QRARIQQEYEEEQEKKREKEEEQRLKNEEHIRLAEERQKEAERKKKEEEEKYNLQLQHYCERDNLIGEET
  KHMRQPSPIVPALQNKIASKLQRPPSVDSIIRSFIHESSMSRAQSPPVPARKNQLRAEEEKKNVIMELSE
  MRKQLRSEERRLQERLLHMDSDDEIPIRKKERNPMDIFDMARHRLQAPVRRQSPKGLDAATFQNVHDFNE
  LKDRDSETRVDLKFMYLDPPRDHHTLEIQQQALLREQQKRLNRIKMQEGAKVDLDAIPSAKVREQRMPRD
  DTSDFLKNSLLESDSAFIGAYGETYPAIEDDVLPPPSQLPSARERRRNKWKGLDIDSSRPNVAPDGLSLK
  SISSVNVDELRVRNEERMRRLNEFHNKPINTDDESSLVDPDDIMKHIGDDGSNSVATEPWLRPGTSETLK
  RFMAEQLNQEQQQIPGKPGTFTWQGLSTAHG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024790
• ORF Size: 3663 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024790.6, NP_079066.5
• RefSeq Size: 4735 bp
• RefSeq ORF: 3666 bp
• Locus ID: 79848
• UniProt ID: Q1MSJ5
• Cytogenetics: 8q13.1-q13.2
• MW: 141.8 kDa
• Gene Summary: This gene encodes a centrosome and spindle pole associated protein. The encoded protein plays a role in cell-cycle progression and spindle organization, regulates cytokinesis, interacts with Nephrocystin 8 and is required for cilia formation. Mutations in this gene result in primary cilia abnormalities and classical Joubert syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]