CSPP1 (NM_024790) Human Untagged Clone

CAT#: SC314850

CSPP1 (untagged)-Human centrosome and spindle pole associated protein 1 (CSPP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSPP1
• Synonyms: CSPP; CSPP-L; JBTS21
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_024790 edited
  ATGGATGAGAGGTTTAGATATGAAAGTGATTTTGATAGAAGACTTTCGAGAGTGTATACA
  AATGACAGGATGCACAGGAACAAACGAGGGAATATGCCTCCTATGGAACATGATGGGGAT
  GTTATAGAACAGTCAAACATAAGAATTTCATCTGCTGAAAATAAAAGTGCTCCAGACAAT
  GAAACATCCAAATCTGCTAATCAAGATACCTGTAGTCCTTTTGCAGGGATGCTCTTTGGA
  GGTGAAGATCGAGAACTTATTCAGAGAAGGAAAGAGAAATACAGACTAGAACTGTTGGAA
  CAAATGGCTGAGCAACAGAGGAACAAGAGACGAGAAAAAGATTTAGAACTCAGGGTTGCA
  GCGTCTGGAGCACAAGACCCTGAGAAATCGCCTGATAGACTAAAGCAGTTTAGTGTGGCA
  CCAAGACACTTTGAAGAGATGATACCACCTGAAAGACCCAGAATAGCTTTCCAGACACCT
  CTCCCTCCTTTATCTGCCCCATCTGTCCCACCCATCCCATCAGTTCATCCTGTTCCTTCT
  CAAAATGAAGATTTGCGCAGTGGACTCAGCAGCGCCCTTGGTGAAATGGTGTCTCCCAGG
  ATTGCACCTCTGCCTCCACCTCCCCTACTACCACCTTTGGCTACTAACTATCGAACTCCT
  TATGATGATGCATACTATTTTTATGGGTCCAGGAATACTTTCGATCCCAGTCTTGCTTAT
  TATGGTTCAGGAATGATGGGCGTACAGCCTGCAGCTTATGTTAGTGCTCCTGTCACCCAC
  CAACTAGCACAACCTGTTGTGAATACGGTTGGACAGAATGAACTGAAGATTACAAGTGAT
  CAAGTGATAAATTCAGGATTGATTTTTGAAGATAAACCGAAACCTTCCAAACAGTCACTT
  CAGTCTTACCAAGAGGCTTTGCAGCAGCAGATTCGGGAAAGAGAAGAAAGAAGGAAGAAA
  GAACGTGAAGAAAAAGAAGAATATGAAGCTAAATTAGAAGCTGAAATGAGAACATATAAT
  CCCTGGGGAAAAGGTGGAGGTGGTGCTCCTCTCAGGGATGCAAAAGGAAATCTGATAAGT
  CATATGCAAACACAGAGCTCTCCTTTTGCTCGGGGAAATGTATTTGGTGAGCCTCCAACT
  GAACTTCAGATTAAACAGCAAGAATTATACAAGAATTTTCTTCGTTTCCAGATTGAGGAA
  AAGAAACAAAGAGAGGAAGCAGAGCGAGAGAGACTGAGAATTGCAGAAGAAAAAGAAGAA
  AGACGGCTTGCAGAACAGAGGGCACGAATTCAGCAGGAGTATGAAGAGGAACAGGAAAAG
  AAAAGAGAGAAAGAGGAGGAGCAAAGGCTAAAAAATGAAGAGCATATTCGGTTAGCTGAA
  GAAAGACAAAAAGAAGCAGAAAGAAAGAAGAAAGAAGAAGAAGAAAAATATAACCTGCAA
  CTTCAGCACTACTGTGAAAGAGACAATTTGATTGGGGAAGAAACAAAGCACATGAGACAG
  CCTTCTCCTATAGTTCCTGCTCTTCAGAACAAAATTGCAAGCAAACTCCAAAGACCTCCT
  TCAGTTGACAGCATCATACGTTCCTTTATTCATGAAAGTTCCATGTCCAGGGCACAGTCA
  CCCCCGGTACCTGCCAGGAAAAATCAGCTCCGTGCAGAAGAGGAGAAAAAAAATGTAATT
  ATGGAATTATCAGAAATGAGAAAACAGCTTCGTAGTGAAGAGAGGCGTCTACAAGAGCGA
  TTGCTACACATGGACAGTGATGATGAAATTCCTATCAGGAAAAAGGAAAGGAATCCCATG
  GATATATTTGATATGGCTAGACATCGGTTGCAAGCTCCTGTCAGAAGACAGTCCCCTAAG
  GGCTTAGACGCTGCCACTTTTCAGAATGTTCATGATTTTAATGAGCTGAAAGATAGAGAT
  TCAGAAACACGAGTTGATCTGAAATTTATGTACCTGGATCCTCCAAGAGATCATCACACC
  TTAGAGATTCAGCAGCAAGCCCTGCTAAGAGAGCAGCAGAAGAGGCTGAACAGAATAAAA
  ATGCAGGAAGGTGCCAAAGTTGACTTAGATGCCATCCCAAGTGCTAAAGTACGAGAGCAA
  AGAATGCCCAGAGATGACACTAGTGATTTCTTGAAAAACTCATTATTGGAATCTGATAGT
  GCTTTTATTGGGGCTTACGGTGAGACATATCCTGCCATTGAAGATGACGTCCTCCCTCCA
  CCATCACAGTTGCCCTCTGCACGGGAGCGCAGGAGGAACAAATGGAAAGGACTAGACATT
  GATAGCAGTCGTCCTAATGTAGCACCAGATGGTCTCTCTCTAAAATCTATATCCAGTGTA
  AATGTTGATGAGCTTAGAGTGAGAAATGAGGAACGAATGCGAAGACTGAATGAATTTCAC
  AATAAACCTATTAATACAGATGATGAGAGTTCACTGGTTGACCCTGATGACATCATGAAA
  CACATAGGGGATGACGGATCAAACTCTGTAGCAACTGAGCCCTGGCTCCGCCCTGGCACT
  TCAGAAACGCTGAAACGTTTCATGGCAGAGCAGCTGAACCAGGAGCAGCAGCAGATTCCT
  GGAAAACCAGGCACTTTCACTTGGCAGGGCCTGTCGACTGCACATGGTTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_024790
• Insert Size: 3300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024790.3, NP_079066.3
• RefSeq Size: 3726 bp
• RefSeq ORF: 2631 bp
• Locus ID: 79848
• UniProt ID: Q1MSJ5
• Cytogenetics: 8q13.1-q13.2
• Gene Summary: This gene encodes a centrosome and spindle pole associated protein. The encoded protein plays a role in cell-cycle progression and spindle organization, regulates cytokinesis, interacts with Nephrocystin 8 and is required for cilia formation. Mutations in this gene result in primary cilia abnormalities and classical Joubert syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
  Transcript Variant: This variant (1) encodes the longest isoform (1, also known as CSPP-L).