TNKS1BP1 (NM_033396) Human Tagged ORF Clone

CAT#: RC223115

TNKS1BP1 (Myc-DDK-tagged)-Human tankyrase 1 binding protein 1, 182kDa (TNKS1BP1)

Product Images

Circular map for RC223115

• 

  Western blot validation of overexpression lysate (Cat# LY409576) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223115 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified TNKS1BP1 protein (Cat# TP323115). The protein was produced from HEK293T cells transfected with TNKS1BP1 cDNA clone (Cat# RC223115) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TNKS1BP1
• Synonyms: TAB182
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223115 representing NM_033396
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGTGTCTACTCTCAGGGAAAGCTCAGCCATGGCTTCCCCACTGCCCCGGGAGATGGAGGAGGAGC
  TGGTGCCTACTGGCTCTGAGCCAGGTGACACTCGGGCCAAACCCCCTGTCAAGCCCAAACCCCGGGCCCT
  GCCTGCCAAGCCAGCCCTGCCTGCCAAACCCAGCCTGCTGGTGCCTGTTGGGCCTCGGCCTCCCCGGGGT
  CCCCTGGCTGAGTTGCCTTCTGCCAGGAAGATGAACATGCTGGCAGGACCCCAGCCCTATGGTGGCAGCA
  AGCGCCCCCTTCCCTTTGCACCAAGGCCTGCGGTTGAGGCCTCCACTGGAGGAGAAGCCACCCAAGAGAC
  TGGGAAAGAGGAGGCTGGGAAAGAGGAGCCACCCCCTTTGACACCCCCAGCTCGATGTGCAGCCCCAGGG
  GGTGTACGGAAGGCCCCTGCCCCTTTCCGCCCAGCCTCAGAGCGCTTCGCGGCCACCACGGTGGAAGAGA
  TCCTGGCCAAGATGGAGCAGCCTCGGAAGGAGGTCCTTGCCAGCCCCGACCGCCTGTGGGGTTCCCGCCT
  CACCTTTAACCACGATGGCAGCTCGCGATATGGCCCCAGGACCTATGGCACGACCACTGCTCCCAGGGAT
  GAGGATGGCAGCACCCTCTTCAGGGGATGGTCCCAGGAGGGGCCAGTAAAGTCTCCAGCAGAGTGCCGGG
  AAGAGCACAGCAAGACCCCTGAGGAGAGGAGCCTTCCTTCCGACCTGGCCTTCAACGGGGACCTGGCTAA
  GGCAGCCAGCTCGGAGCTACCTGCTGATATTTCCAAGCCCTGGATTCCCTCAAGTCCAGCCCCCTCCTCA
  GAGAATGGAGGCCCTGCCAGCCCAGGCCTCCCCGCAGAAGCCTCAGGCTCAGGCCCTGGCTCTCCCCATC
  TTCACCCGCCTGATAAGAGTTCTCCCTGCCACTCACAGCTTCTGGAAGCCCAGACTCCTGAAGCTTCCCA
  GGCTTCTCCCTGCCCCGCTGTGACTCCATCAGCTCCAAGTGCAGCCCTGCCTGACGAGGGCTCCCGCCAC
  ACCCCCAGCCCGGGGCTCCCTGCCGAGGGGGCTCCAGAGGCCCCCAGACCCAGCAGCCCACCCCCTGAGG
  TCTTGGAGCCCCATAGCCTGGATCAGCCCCCTGCCACCTCACCCCGGCCCCTGATCGAGGTGGGTGAGTT
  GCTGGATCTCACTCGGACGTTTCCATCTGGCGGGGAGGAGGAGGCCAAGGGTGACGCACACCTCCGCCCC
  ACCAGCCTGGTTCAGCGCCGATTCTCTGAAGGTGTGCTCCAGTCACCCAGTCAGGACCAGGAGAAGCTGG
  GGGGCTCGCTGGCTGCCCTGCCCCAAGGCCAGGGGAGCCAGTTGGCCCTGGATCGTCCCTTTGGGGCAGA
  GTCCAACTGGAGCTTATCACAGTCCTTCGAATGGACCTTCCCCACGAGGCCCTCGGGTCTGGGCGTGTGG
  CGGCTGGACTCCCCGCCTCCCTCCCCCATCACTGAAGCCAGTGAGGCCGCCGAGGCTGCTGAGGCTGGCA
  ACTTGGCCGTTTCCAGCAGGGAAGAAGGAGTGTCTCAGCAGGGGCAAGGGGCTGGGTCAGCTCCAAGTGG
  GTCAGGAAGTTCCTGGGTGCAGGGGGATGATCCAAGCATGTCCCTCACCCAGAAGGGCGATGGGGAGAGT
  CAACCTCAATTCCCAGCTGTTCCCCTTGAGCCCCTGCCTACAACTGAGGGCACACCTGGATTACCTTTGC
  AGCAGGCAGAGGAGAGATACGAGTCGCAGGAGCCCTTGGCTGGACAGGAGTCCCCTCTCCCCCTGGCTAC
  CAGGGAGGCAGCCTTGCCCATCCTGGAGCCAGTCCTGGGGCAGGAGCAGCCAGCAGCCCCTGACCAGCCC
  TGTGTTCTCTTTGCTGATGCCCCTGAGCCTGGACAGGCACTGCCTGTTGAGGAGGAGGCCGTGACCCTAG
  CCCGGGCTGAGACCACCCAAGCCAGGACAGAGGCTCAAGACTTGTGTAGGGCATCCCCCGAGCCTCCAGG
  CCCTGAAAGCAGCTCCCGCTGGCTGGACGACCTCCTGGCTTCACCACCACCCAGTGGTGGCGGTGCAAGG
  CGGGGAGCTGGAGCTGAGCTGAAGGACACACAGTCCCCAAGTACCTGCTCTGAGGGACTCCTTGGCTGGT
  CCCAGAAAGATCTGCAGAGTGAATTTGGGATCACAGGAGACCCACAGCCCAGCAGTTTCAGTCCTTCCAG
  CTGGTGTCAAGGTGCTTCTCAGGACTATGGCCTTGGGGGTGCAAGCCCTAGAGGAGACCCAGGTCTCGGA
  GAGAGGGACTGGACCAGCAAGTATGGGCAAGGAGCAGGGGAAGGGAGCACCAGGGAGTGGGCCAGCAGGT
  GTGGCATCGGCCAGGAGGAGATGGAGGCCAGCAGCAGCCAAGACCAGAGTAAAGTGTCTGCCCCAGGGGT
  GCTCACAGCCCAGGACCGGGTAGTTGGAAAGCCAGCCCAGCTTGGCACTCAGCGGAGCCAGGAGGCAGAT
  GTTCAGGACTGGGAGTTCAGAAAGAGGGATTCCCAGGGCACTTACTCCAGCCGGGATGCAGAACTCCAGG
  ACCAGGAATTCGGAAAGAGAGATTCACTGGGTACCTACAGTAGTCGAGATGTAAGCCTTGGGGACTGGGA
  ATTTGGGAAGAGAGATTCTCTGGGTGCTTATGCCAGCCAAGATGCCAACGAGCAGGGCCAAGATTTGGGG
  AAGAGGGACCACCATGGTAGGTACAGCAGCCAGGATGCCGATGAGCAGGACTGGGAGTTTCAGAAGAGAG
  ATGTGTCACTCGGCACCTATGGCAGCCGGGCTGCGGAGCCACAGGAACAGGAGTTTGGGAAGAGCGCTTG
  GATAAGGGACTACAGCAGTGGTGGCAGCTCCAGGACCCTTGACGCCCAGGACAGAAGCTTTGGAACGAGA
  CCCCTGAGCTCTGGGTTCAGCCCCGAGGAAGCCCAGCAACAGGATGAGGAATTTGAGAAGAAGATTCCAA
  GTGTGGAAGACAGCCTTGGAGAGGGCAGCAGGGATGCTGGCCGGCCAGGAGAGAGAGGATCCGGGGGCTT
  GTTCAGTCCTAGCACTGCCCACGTGCCGGATGGGGCACTCGGGCAGAGAGACCAGAGCAGCTGGCAAAAC
  AGTGATGCTAGCCAGGAGGTGGGAGGGCATCAGGAGAGACAGCAGGCAGGGGCTCAGGGCCCTGGCAGTG
  CTGACCTGGAAGATGGGGAGATGGGAAAGCGAGGCTGGGTCGGTGAGTTTAGCCTCAGTGTTGGCCCCCA
  GCGAGAGGCAGCATTTAGCCCAGGGCAGCAGGACTGGAGCCGGGACTTCTGCATCGAGGCCAGTGAGAGG
  AGCTATCAGTTTGGCATCATTGGCAACGACAGAGTGAGTGGTGCTGGCTTTAGCCCTTCTAGCAAGATGG
  AAGGTGGTCACTTTGTGCCTCCTGGGAAGACCACAGCTGGCTCGGTGGACTGGACTGACCAGCTGGGTCT
  CAGGAACTTGGAAGTGTCCAGCTGTGTGGGTTCTGGGGGCTCGAGCGAGGCCAGGGAGAGTGCCGTGGGA
  CAGATGGGCTGGTCAGGTGGCCTGAGCTTGAGAGACATGAACCTGACCGGCTGTTTGGAAAGTGGAGGGT
  CTGAAGAGCCGGGGGGAATCGGAGTTGGGGAGAAGGACTGGACTTCTGATGTTAATGTGAAGAGCAAAGA
  TTTGGCTGAGGTCGGGGAGGGAGGAGGCCACAGCCAGGCCAGAGAGAGTGGCGTGGGGCAGACTGACTGG
  TCAGGTGTGGAGGCCGGAGAGTTCCTTAAATCAAGGGAGCGTGGAGTTGGACAGGCAGACTGGACACCTG
  ACCTTGGGCTGAGAAACATGGCCCCAGGGGCAGTCTGCAGTCCTGGAGAGTCCAAAGAGCTTGGGGTGGG
  CCAGATGGACTGGGGTAACAATCTGGGCCTGAGGGATTTGGAGGTGACCTGTGACCCAGACTCTGGAGGT
  TCTCAGGGGCTACGGGGATGTGGAGTGGGGCAGATGGACTGGACCCAGGACTTGGCGCCCCAGAATGTGG
  AGCTCTTTGGGGCTCCAAGTGAAGCCAGGGAGCATGGGGTGGGCGGGGTGAGCCAGTGCCCAGAGCCCGG
  CCTGAGGCACAATGGCAGCTTGTCTCCTGGCCTGGAGGCCAGAGACCCCTTGGAGGCCAGGGAGCTGGGG
  GTTGGTGAGACAAGTGGGCCAGAGACCCAGGGTGAAGATTACTCCTCGTCTTCCTTGGAGCCACACCCTG
  CAGACCCTGGAATGGAGACAGGAGAAGCCCTCAGCTTCGGAGCAAGCCCTGGCAGGTGCCCGGCCCGCCC
  CCCACCCTCCGGCTCCCAGGGCCTGCTGGAGGAGATGCTGGCAGCCAGCAGCTCCAAGGCGGTGGCTCGG
  AGGGAGTCAGCGGCCTCGGGCCTTGGGGGCCTGTTGGAGGAGGAAGGAGCCGGGGCAGGTGCTGCCCAAG
  AGGAGGTGCTGGAGCCTGGCAGGGACTCTCCACCCTCCTGGAGGCCGCAGCCTGATGGTGAGGCCAGCCA
  GACAGAAGACGTGGATGGCACCTGGGGCTCTTCAGCAGCCAGGTGGAGCGATCAGGGGCCAGCACAGACT
  TCTCGGCGACCCTCCCAAGGCCCTCCTGCCAGATCCCCCAGTCAGGACTTCTCCTTCATTGAGGACACCG
  AGATCCTCGACAGTGCCATGTATCGGAGCCGTGCCAACTTGGGGCGCAAGCGTGGGCACCGGGCCCCGGT
  CATTCGGCCTGGGGGTACCTTGGGCCTGTCGGAGGCAGCAGACTCGGATGCACACCTGTTCCAGGACTCT
  ACAGAGCCACGGGCATCTCGGGTGCCATCTTCAGATGAAGAGGTAGTGGAGGAACCTCAGAGCCGCCGGA
  CACGGATGTCGTTGGGCACCAAGGGGCTGAAAGTCAACCTCTTTCCTGGCCTGAGCCCCTCAGCCCTGAA
  GGCCAAGCTGCGCCCCCGGAATCGCTCAGCTGAGGAGGGAGAGCTGGCTGAGAGCAAGTCGAGCCAGAAG
  GAGTCCGCGGTCCAGCGTTCGAAATCCTGCAAGGTCCCAGGACTGGGAAAGCCCCTCACGTTACCTCCCA
  AGCCAGAGAAATCCTCAGGGTCAGAAGGATCGTCGCCCAACTGGCTTCAAGCCCTGAAACTGAAGAAGAA
  GAAGGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223115 representing NM_033396
  Red=Cloning site Green=Tags(s)
  
  MKVSTLRESSAMASPLPREMEEELVPTGSEPGDTRAKPPVKPKPRALPAKPALPAKPSLLVPVGPRPPRG
  PLAELPSARKMNMLAGPQPYGGSKRPLPFAPRPAVEASTGGEATQETGKEEAGKEEPPPLTPPARCAAPG
  GVRKAPAPFRPASERFAATTVEEILAKMEQPRKEVLASPDRLWGSRLTFNHDGSSRYGPRTYGTTTAPRD
  EDGSTLFRGWSQEGPVKSPAECREEHSKTPEERSLPSDLAFNGDLAKAASSELPADISKPWIPSSPAPSS
  ENGGPASPGLPAEASGSGPGSPHLHPPDKSSPCHSQLLEAQTPEASQASPCPAVTPSAPSAALPDEGSRH
  TPSPGLPAEGAPEAPRPSSPPPEVLEPHSLDQPPATSPRPLIEVGELLDLTRTFPSGGEEEAKGDAHLRP
  TSLVQRRFSEGVLQSPSQDQEKLGGSLAALPQGQGSQLALDRPFGAESNWSLSQSFEWTFPTRPSGLGVW
  RLDSPPPSPITEASEAAEAAEAGNLAVSSREEGVSQQGQGAGSAPSGSGSSWVQGDDPSMSLTQKGDGES
  QPQFPAVPLEPLPTTEGTPGLPLQQAEERYESQEPLAGQESPLPLATREAALPILEPVLGQEQPAAPDQP
  CVLFADAPEPGQALPVEEEAVTLARAETTQARTEAQDLCRASPEPPGPESSSRWLDDLLASPPPSGGGAR
  RGAGAELKDTQSPSTCSEGLLGWSQKDLQSEFGITGDPQPSSFSPSSWCQGASQDYGLGGASPRGDPGLG
  ERDWTSKYGQGAGEGSTREWASRCGIGQEEMEASSSQDQSKVSAPGVLTAQDRVVGKPAQLGTQRSQEAD
  VQDWEFRKRDSQGTYSSRDAELQDQEFGKRDSLGTYSSRDVSLGDWEFGKRDSLGAYASQDANEQGQDLG
  KRDHHGRYSSQDADEQDWEFQKRDVSLGTYGSRAAEPQEQEFGKSAWIRDYSSGGSSRTLDAQDRSFGTR
  PLSSGFSPEEAQQQDEEFEKKIPSVEDSLGEGSRDAGRPGERGSGGLFSPSTAHVPDGALGQRDQSSWQN
  SDASQEVGGHQERQQAGAQGPGSADLEDGEMGKRGWVGEFSLSVGPQREAAFSPGQQDWSRDFCIEASER
  SYQFGIIGNDRVSGAGFSPSSKMEGGHFVPPGKTTAGSVDWTDQLGLRNLEVSSCVGSGGSSEARESAVG
  QMGWSGGLSLRDMNLTGCLESGGSEEPGGIGVGEKDWTSDVNVKSKDLAEVGEGGGHSQARESGVGQTDW
  SGVEAGEFLKSRERGVGQADWTPDLGLRNMAPGAVCSPGESKELGVGQMDWGNNLGLRDLEVTCDPDSGG
  SQGLRGCGVGQMDWTQDLAPQNVELFGAPSEAREHGVGGVSQCPEPGLRHNGSLSPGLEARDPLEARELG
  VGETSGPETQGEDYSSSSLEPHPADPGMETGEALSFGASPGRCPARPPPSGSQGLLEEMLAASSSKAVAR
  RESAASGLGGLLEEEGAGAGAAQEEVLEPGRDSPPSWRPQPDGEASQTEDVDGTWGSSAARWSDQGPAQT
  SRRPSQGPPARSPSQDFSFIEDTEILDSAMYRSRANLGRKRGHRAPVIRPGGTLGLSEAADSDAHLFQDS
  TEPRASRVPSSDEEVVEEPQSRRTRMSLGTKGLKVNLFPGLSPSALKAKLRPRNRSAEEGELAESKSSQK
  ESAVQRSKSCKVPGLGKPLTLPPKPEKSSGSEGSSPNWLQALKLKKKKV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033396
• ORF Size: 5187 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033396.3
• RefSeq Size: 5947 bp
• RefSeq ORF: 5190 bp
• Locus ID: 85456
• UniProt ID: Q9C0C2
• Cytogenetics: 11q12.1
• MW: 181.6 kDa