ANKFY1 (NM_020740) Human Tagged ORF Clone

CAT#: RC223110

ANKFY1 (Myc-DDK-tagged)-Human ankyrin repeat and FYVE domain containing 1 (ANKFY1), transcript variant 2

Product Images

Circular map for RC223110

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  Western blot validation of overexpression lysate (Cat# LY412365) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223110 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ANKFY1 protein (Cat# TP323110). The protein was produced from HEK293T cells transfected with ANKFY1 cDNA clone (Cat# RC223110) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANKFY1
• Synonyms: ANKHZN; DKFZp686M19106; KIAA1255; ZFYVE14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223110 representing NM_020740
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGAGGAGGTGGCCAAGTTGGAGAAGCACTTGATGCTTCTGCGGCAGGAGTATGTCAAGCTGC
  AGAAGAAGCTGGCGGAGACAGAGAAGCGCTGCGCTCTCTTGGCTGCGCAGGCAAACAAGGAGAGCAGCAG
  CGAGTCCTTCATCAGCCGTCTGCTGGCCATCGTGGCAGACCTCTACGAGCAGGAGCAGTACAGCGATCTG
  AAGATAAAGGTTGGGGACAGGCACATCAGTGCTCACAAGTTTGTCCTGGCAGCCCGCAGTGACAGCTGGA
  GTCTGGCTAACTTGTCTTCCACTAAAGAGTTGGACCTGTCAGATGCTAATCCTGAGGTGACGATGACAAT
  GCTTCGCTGGATCTATACAGATGAGCTGGAGTTCAGAGAGGATGATGTGTTCCTGACTGAACTGATGAAA
  CTAGCAAATCGGTTTCAGCTACAGCTCCTCAGGGAGAGATGTGAGAAGGGTGTTATGTCTCTAGTGAATG
  TCAGGAACTGTATTCGCTTCTACCAGACGGCAGAGGAGCTGAATGCCAGCACACTGATGAACTACTGTGC
  AGAAATTATTGCAAGTCATTGGGACGACCTGAGGAAGGAGGATTTCAGCAGCATGAGCGCTCAGTTGTTA
  TACAAAATGATCAAATCCAAGACAGAGTACCCGCTACATAAAGCCATCAAAGTGGAGAGAGAAGACGTGG
  TCTTCCTGTATCTGATTGAAATGGATTCCCAGCTCCCTGGGAAGCTGAATGAAGCGGATCATAACGGAGA
  TCTGGCATTAGATCTAGCCCTCTCACGACGACTGGAGAGTATTGCCACCACGCTGGTTAGTCACAAAGCT
  GATGTGGACATGGTGGACAAGAGTGGCTGGAGCTTGTTACACAAAGGAATCCAAAGAGGAGATCTCTTTG
  CTGCCACTTTCCTCATTAAGAATGGGGCCTTTGTCAACGCTGCTACACTGGGTGCCCAGGAGACACCACT
  GCACCTTGTGGCCTTGTACAGTTCAAAGAAACACTCAGCAGATGTGATGTCTGAGATGGCGCAGATTGCA
  GAGGCCCTTCTGCAGGCTGGTGCCAACCCCAACATGCAGGACAGCAAGGGGAGGACTCCTTTACATGTGT
  CCATCATGGCCGGGAATGAATATGTGTTCAGTCAGCTGCTGCAGTGCAAACAACTAGATTTAGAACTCAA
  AGACCACGAGGGCAGCACGGCTCTGTGGCTGGCAGTGCAGCATATCACAGTGTCTTCTGACCAGTCTGTG
  AACCCCTTCGAAGATGTCCCCGTGGTAAATGGGACTTCATTTGATGAGAACAGCTTTGCAGCCAGACTCA
  TCCAGCGCGGCAGCCACACAGACGCACCTGACACGGCGACAGGTAAAGCACGTGCCTCCCGAAGGGGCGA
  CGCTGGGGTCTGCAGGCGCCAGGAAATGGCATGTAAATGCCTTCACCCCAAATTTAGAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223110 representing NM_020740
  Red=Cloning site Green=Tags(s)
  
  MAEEEVAKLEKHLMLLRQEYVKLQKKLAETEKRCALLAAQANKESSSESFISRLLAIVADLYEQEQYSDL
  KIKVGDRHISAHKFVLAARSDSWSLANLSSTKELDLSDANPEVTMTMLRWIYTDELEFREDDVFLTELMK
  LANRFQLQLLRERCEKGVMSLVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMSAQLL
  YKMIKSKTEYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNEADHNGDLALDLALSRRLESIATTLVSHKA
  DVDMVDKSGWSLLHKGIQRGDLFAATFLIKNGAFVNAATLGAQETPLHLVALYSSKKHSADVMSEMAQIA
  EALLQAGANPNMQDSKGRTPLHVSIMAGNEYVFSQLLQCKQLDLELKDHEGSTALWLAVQHITVSSDQSV
  NPFEDVPVVNGTSFDENSFAARLIQRGSHTDAPDTATGKARASRRGDAGVCRRQEMACKCLHPKFRN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020740
• ORF Size: 1461 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020740.2, NP_065791.1
• RefSeq Size: 1904 bp
• RefSeq ORF: 1463 bp
• Locus ID: 51479
• Cytogenetics: 17p13.2
• MW: 54.6 kDa
• Gene Summary: This gene encodes a cytoplasmic protein that contains a coiled-coil structure and a BTB/POZ domain at its N-terminus, ankyrin repeats in the middle portion, and a FYVE-finger motif at its C-terminus. This protein belongs to a subgroup of double zinc finger proteins which may be involved in vesicle or protein transport. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Apr 2012]