TNK1 (NM_003985) Human Tagged ORF Clone

CAT#: RC223073

TNK1 (Myc-DDK-tagged)-Human tyrosine kinase, non-receptor, 1 (TNK1)

Product Images

Circular map for RC223073

• 

  Western blot validation of overexpression lysate (Cat# LY401305) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223073 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TNK1
• Synonyms: KOS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223073 representing NM_003985
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTCCTGAGGCTGGCTCTCTGTGGCTACTGAAGCTGCTCCGGGACATCCAGTTGGCCCAGTTTTACT
  GGCCCATCCTTGAGGAGCTTAATGTCACTCGGCCAGAGCACTTCGACTTTGTAAAGCCTGAGGACCTGGA
  CGGCATTGGCATGGGCCGGCCTGCCCAGCGCAGACTGTCCGAAGCTCTGAAAAGGCTACGTTCTGGGCCT
  AAGTCTAAGAACTGGGTCTACAAGATCCTTGGAGGTTTTGCCCCTGAGCACAAGGAGCCCACCCTGCCCT
  CGGACAGCCCACGGCACCTCCCTGAGCCAGAGGGGGGCCTCAAGTGTCTGATCCCAGAGGGTGCTGTTTG
  CAGAGGGGAGCTGCTGGGTTCAGGCTGCTTCGGTGTGGTGCACCGAGGGCTGTGGACGCTGCCCAGTGGC
  AAGAGTGTCCCAGTGGCTGTCAAGTCCCTCCGGGTAGGTCCCGAAGGCCCGATGGGCACAGAACTGGGGG
  ACTTCCTGCGAGAGGTATCGGTCATGATGAACTTGGAGCACCCACACGTGCTGCGTCTGCACGGCCTTGT
  ACTGGGCCAGCCTCTGCAGATGGTGATGGAGCTGGCGCCACTGGGCTCCCTGCACGCGCGCCTAACGGCC
  CCGGCCCCGACACCCCCGCTGCTCGTGGCCCTGCTCTGCCTCTTCCTGCGGCAGCTGGCGGGAGCCATGG
  CGTACCTGGGGGCCCGCGGGCTGGTGCACCGAGACCTCGCTACGCGCAACCTACTGCTGGCGTCGCCGCG
  CACCATCAAGGTGGCTGACTTCGGGCTGGTGCGGCCTCTGGGCGGTGCCCGGGGCCGCTACGTCATGGGC
  GGGCCCCGCCCTATCCCCTACGCCTGGTGTGCCCCAGAGAGCCTGCGCCACGGAGCCTTCTCGTCTGCCT
  CGGACGTGTGGATGTTTGGGGTGACGCTGTGGGAGATGTTCTCCGGGGGCGAGGAACCCTGGGCCGGGGT
  CCCACCGTACCTCATCCTGCAGCGGCTGGAGGACAGAGCCCGGCTGCCTAGGCCTCCCCTCTGCTCCAGG
  GCCCTCTACTCCCTCGCCTTGCGCTGCTGGGCCCCCCACCCTGCCGACCGGCCTAGCTTTTCCCACCTGG
  AGGGGCTGCTGCAAGAGGCCGGGCCTTCGGAAGCATGTTGTGTGAGGGATGTCACAGAACCAGGCGCCCT
  GAGGATGGAGACTGGTGACCCCATCACAGTCATCGAGGGCAGCCCCGACTCCACAATCTGGAAGGGCCAG
  AATGGTCGCACCTTCAAAGTGGGCAGCTTCCCAGCCTCGGCAGTGACGCTGGCAGATGCGGGGGGCTTGC
  CAGCCACCCGTCCAGTCCACAGAGGCACCCCTGCCCGGGGAGATCAACACCCAGGAAGCATAGATGGAGA
  CAGAAAGAAGGCAAATCTTTGGGATGCGCCCCCAGCACGGGGCCAGAGGAGGAACATGCCCCTGGAGAGG
  ATGAAAGGCATTTCCAGGAGTCTGGAGTCAGTTCTGTCCCTCGGTCCTCGTCCCACAGGGGGTGGTTCAA
  GCCCCCCTGAAATTCGACAAGCCAGAGCTGTGCCCCAGGGACCTCCAGGCCTGCCTCCACGCCCACCTTT
  ATCCTCTAGCTCTCCTCAGCCCAGCCAGCCCTCTAGGGAGAGGCTTCCCTGGCCCAAAAGAAAACCCCCA
  CACAATCACCCCATGGGAATGCCTGGAGCCCGTAAAGCCGCTGCCCTCTCTGGAGGCCTCTTGTCCGATC
  CTGAGTTGCAGAGGAAGATTATGGAGATGGAGCTGAGTGTGCATGGGGTCACCCACCAGGAGTGCCAGAC
  AGCACTAGGAGCCACTGGGGGAGATGTGGTTTCTGCCATCCGGAACCTCAAGGTAGATCAGCTCTTCCAC
  CTGAGTAGCCGGTCCAGAGCTGACTGCTGGCGCATCCTGGAGCATTACCAGTGGGACCTCTCAGCTGCCA
  GCCGCTATGTCCTGGCCAGGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223073 representing NM_003985
  Red=Cloning site Green=Tags(s)
  
  MLPEAGSLWLLKLLRDIQLAQFYWPILEELNVTRPEHFDFVKPEDLDGIGMGRPAQRRLSEALKRLRSGP
  KSKNWVYKILGGFAPEHKEPTLPSDSPRHLPEPEGGLKCLIPEGAVCRGELLGSGCFGVVHRGLWTLPSG
  KSVPVAVKSLRVGPEGPMGTELGDFLREVSVMMNLEHPHVLRLHGLVLGQPLQMVMELAPLGSLHARLTA
  PAPTPPLLVALLCLFLRQLAGAMAYLGARGLVHRDLATRNLLLASPRTIKVADFGLVRPLGGARGRYVMG
  GPRPIPYAWCAPESLRHGAFSSASDVWMFGVTLWEMFSGGEEPWAGVPPYLILQRLEDRARLPRPPLCSR
  ALYSLALRCWAPHPADRPSFSHLEGLLQEAGPSEACCVRDVTEPGALRMETGDPITVIEGSPDSTIWKGQ
  NGRTFKVGSFPASAVTLADAGGLPATRPVHRGTPARGDQHPGSIDGDRKKANLWDAPPARGQRRNMPLER
  MKGISRSLESVLSLGPRPTGGGSSPPEIRQARAVPQGPPGLPPRPPLSSSSPQPSQPSRERLPWPKRKPP
  HNHPMGMPGARKAAALSGGLLSDPELQRKIMEMELSVHGVTHQECQTALGATGGDVVSAIRNLKVDQLFH
  LSSRSRADCWRILEHYQWDLSAASRYVLARP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003985
• ORF Size: 1983 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003985.5
• RefSeq Size: 2771 bp
• RefSeq ORF: 1986 bp
• Locus ID: 8711
• UniProt ID: Q13470
• Cytogenetics: 17p13.1
• Domains: pkinase, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 71.7 kDa
• Gene Summary: The protein encoded by this gene belongs to the tyrosine protein kinase family. Tyrosine protein kinases are important regulators of intracellular signal transduction pathways, mediating cellular proliferation, survival, and development. This gene is highly expressed in fetal tissues and at lower levels in few adult tissues, thus may function in signaling pathways utilized broadly during fetal development, and more selectively in adult tissues. It plays a negative regulatory role in the Ras-Raf1-MAPK pathway, and knockout mice have been shown to develop spontaneous tumors, suggesting a role as a tumor suppressor gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]