GRIK2 (NM_175768) Human Tagged ORF Clone

CAT#: RC223048

GRIK2 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 2

Product Images

Circular map for RC223048

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  Western blot validation of overexpression lysate (Cat# LY406220) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223048 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GRIK2 protein (Cat# TP323048). The protein was produced from HEK293T cells transfected with GRIK2 cDNA clone (Cat# RC223048) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRIK2
• Synonyms: EAA4; GLR6; GluK2; GLUK6; GLUR6; MRT6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223048 representing NM_175768
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGATTATTTTCCCGATTCTAAGTAATCCAGTCTTCAGGCGCACCGTTAAACTCCTGCTCTGTTTAC
  TGTGGATTGGATATTCTCAAGGAACCACACATGTATTAAGATTTGGTGGTATTTTTGAATATGTGGAATC
  TGGCCCAATGGGAGCTGAGGAACTTGCATTCAGATTTGCTGTGAACACAATTAACAGAAACAGAACATTG
  CTACCCAATACTACCCTTACCTATGATACCCAGAAGATAAACCTTTATGATAGTTTTGAAGCATCCAAGA
  AAGCCTGTGATCAGCTGTCTCTTGGGGTGGCTGCCATCTTCGGGCCTTCACACAGCTCATCAGCAAACGC
  AGTGCAGTCCATCTGCAATGCTCTGGGAGTTCCCCACATACAGACCCGCTGGAAGCACCAGGTGTCAGAC
  AACAAAGATTCCTTCTATGTCAGTCTCTACCCAGACTTCTCTTCACTCAGCCGTGCCATTTTAGACCTGG
  TGCAGTTTTTCAAGTGGAAAACCGTCACGGTTGTGTATGATGACAGCACTGGTCTCATTCGTTTGCAAGA
  GCTCATCAAAGCTCCATCAAGGTATAATCTTCGACTCAAAATTCGTCAGTTACCTGCTGATACAAAGGAT
  GCAAAACCCTTACTAAAAGAAATGAAAAGAGGCAAGGAGTTTCATGTAATCTTTGATTGTAGCCATGAAA
  TGGCAGCAGGCATTTTAAAACAGGCATTAGCTATGGGAATGATGACAGAATACTATCATTATATCTTTAC
  CACTCTGGACCTCTTTGCTCTTGATGTTGAGCCCTACCGATACAGTGGTGTTAACATGACAGGGTTCAGA
  ATATTAAATACAGAAAATACCCAAGTCTCCTCCATCATTGAAAAGTGGTCGATGGAACGATTGCAGGCAC
  CTCCGAAACCCGATTCAGGTTTGCTGGATGGATTTATGACGACTGATGCTGCTCTAATGTATGATGCTGT
  GCATGTGGTGTCTGTGGCCGTTCAACAGTTTCCCCAGATGACAGTCAGTTCCTTGCAGTGTAATCGACAT
  AAACCCTGGCGCTTCGGGACCCGCTTTATGAGTCTAATTAAAGAGGCACATTGGGAAGGCCTCACAGGCA
  GAATAACTTTCAACAAAACCAATGGCTTGAGAACAGATTTTGATTTGGATGTGATCAGTCTGAAGGAAGA
  AGGTCTAGAAAAGATTGGAACGTGGGATCCAGCCAGTGGCCTGAATATGACAGAAAGTCAAAAGGGAAAG
  CCAGCGAACATCACAGATTCCTTATCCAATCGTTCTTTGATTGTTACCACCATTTTGGAAGAGCCTTATG
  TCCTTTTTAAGAAGTCTGACAAACCTCTCTATGGTAATGATCGATTTGAAGGCTATTGCATTGATCTCCT
  CAGAGAGTTATCTACAATCCTTGGCTTTACATATGAAATTAGACTTGTGGAAGATGGGAAATATGGAGCC
  CAGGATGATGCCAATGGACAATGGAATGGAATGGTTCGTGAACTAATTGATCATAAAGCTGACCTTGCAG
  TTGCTCCACTGGCTATTACCTATGTTCGAGAGAAGGTCATCGACTTTTCCAAGCCCTTTATGACACTTGG
  AATAAGTATTTTGTACCGCAAGCCCAATGGTACAAACCCAGGCGTCTTCTCCTTCCTGAATCCTCTCTCC
  CCTGATATCTGGATGTATATTCTGCTGGCTTACTTGGGTGTCAGTTGTGTGCTCTTTGTCATAGCCAGGT
  TTAGTCCTTATGAGTGGTATAATCCACACCCTTGCAACCCTGACTCAGACGTGGTGGAAAACAATTTTAC
  CTTGCTAAATAGTTTCTGGTTTGGAGTTGGAGCTCTCATGCAGCAAGGTTCTGAGCTCATGCCCAAAGCA
  CTGTCCACCAGGATAGTGGGAGGCATTTGGTGGTTTTTCACACTTATCATCATTTCTTCGTATACTGCTA
  ACTTAGCCGCCTTTCTGACAGTGGAACGCATGGAATCCCCTATTGACTCTGCTGATGATTTAGCTAAACA
  AACCAAGATAGAATATGGAGCAGTAGAGGATGGTGCAACCATGACTTTTTTCAAGAAATCAAAAATCTCC
  ACGTATGACAAAATGTGGGCCTTTATGAGTAGCAGAAGGCAGTCAGTGCTGGTCAAAAGTAATGAAGAAG
  GAATCCAGCGAGTCCTCACCTCTGATTATGCTTTCCTAATGGAGTCAACAACCATCGAGTTTGTTACCCA
  GCGGAACTGTAACCTGACACAGATTGGCGGCCTTATAGACTCTAAAGGTTATGGCGTTGGCACTCCCATG
  GGTTCTCCATATCGAGACAAAATTACCATAGCAATTCTTCAGCTGCAAGAGGAAGGCAAACTGCATATGA
  TGAAGGAGAAATGGTGGAGGGGCAATGGTTGCCCAGAAGAGGAGAGCAAAGAGGCCAGTGCCCTGGGGGT
  TCAGAATATTGGTGGCATCTTCATTGTTCTGGCAGCCGGCTTGGTGCTTTCAGTTTTTGTGGCAGTGGGA
  GAATTTTTATACAAATCCAAAAAAAACGCTCAATTGGAAAAGGAATCTTCTATTTGGTTAGTGCCACCAT
  ACCATCCAGACACTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223048 representing NM_175768
  Red=Cloning site Green=Tags(s)
  
  MKIIFPILSNPVFRRTVKLLLCLLWIGYSQGTTHVLRFGGIFEYVESGPMGAEELAFRFAVNTINRNRTL
  LPNTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSD
  NKDSFYVSLYPDFSSLSRAILDLVQFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQLPADTKD
  AKPLLKEMKRGKEFHVIFDCSHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMTGFR
  ILNTENTQVSSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQMTVSSLQCNRH
  KPWRFGTRFMSLIKEAHWEGLTGRITFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPASGLNMTESQKGK
  PANITDSLSNRSLIVTTILEEPYVLFKKSDKPLYGNDRFEGYCIDLLRELSTILGFTYEIRLVEDGKYGA
  QDDANGQWNGMVRELIDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLS
  PDIWMYILLAYLGVSCVLFVIARFSPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQGSELMPKA
  LSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVEDGATMTFFKKSKIS
  TYDKMWAFMSSRRQSVLVKSNEEGIQRVLTSDYAFLMESTTIEFVTQRNCNLTQIGGLIDSKGYGVGTPM
  GSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEEESKEASALGVQNIGGIFIVLAAGLVLSVFVAVG
  EFLYKSKKNAQLEKESSIWLVPPYHPDTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175768
• ORF Size: 2607 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175768.3
• RefSeq Size: 3409 bp
• RefSeq ORF: 2610 bp
• Locus ID: 2898
• UniProt ID: Q13002
• Cytogenetics: 6q16.3
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 94.3 kDa
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive cognitive disability. [provided by RefSeq, Jul 2008]