GRIK2 (NM_175768) Human Untagged Clone

CAT#: SC310072

GRIK2 (untagged)-Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRIK2
• Synonyms: EAA4; GLR6; GluK2; GLUK6; GLUR6; MRT6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_175768 edited
  ATTTCTCCCGGATGCTCTCCGACTAACATGGATGTCCCACCATTCCTTGCAGTGGAAGGT
  TGTTCCTTGGCGCAGTGAGTGAAGAACATGCAGCGATTGCTAATGGGTTTGGGAAGCGGA
  GACTCCTTCCTCTCTCTATGACCATGCCGTGATCGTGTCTGCGGTCACCACTCGACGCAT
  CCTCATTTCTACCCGAACCCAGGAGCCGAACGCTAGATCGGGGAAGTGGGTGCCGTGCGT
  GTGGGCACAGAAACACCATGAAGATTATTTTCCCGATTCTAAGTAATCCAGTCTTCAGGC
  GCACCGTTAAACTCCTGCTCTGTTTACTGTGGATTGGATATTCTCAAGGAACCACACATG
  TATTAAGATTTGGTGGTATTTTTGAATATGTGGAATCTGGCCCAATGGGAGCTGAGGAAC
  TTGCATTCAGATTTGCTGTGAACACAATTAACAGAAACAGAACATTGCTACCCAATACTA
  CCCTTACCTATGATACCCAGAAGATAAACCTTTATGATAGTTTTGAAGCATCCAAGAAAG
  CCTGTGATCAGCTGTCTCTTGGGGTGGCTGCCATCTTCGGGCCTTCACACAGCTCATCAG
  CAAACGCAGTGCAGTCCATCTGCAATGCTCTGGGAGTTCCCCACATACAGACCCGCTGGA
  AGCACCAGGTGTCAGACAACAAAGATTCCTTCTATGTCAGTCTCTACCCAGACTTCTCTT
  CACTCAGCCGTGCCATTTTAGACCTGGTGCAGTTTTTCAAGTGGAAAACCGTCACGGTTG
  TGTATGATGACAGCACTGGTCTCATTCGTTTGCAAGAGCTCATCAAAGCTCCATCAAGGT
  ATAATCTTCGACTCAAAATTCGTCAGTTACCTGCTGATACAAAGGATGCAAAACCCTTAC
  TAAAAGAAATGAAAAGAGGCAAGGAGTTTCATGTAATCTTTGATTGTAGCCATGAAATGG
  CAGCAGGCATTTTAAAACAGGCATTAGCTATGGGAATGATGACAGAATACTATCATTATA
  TCTTTACCACTCTGGACCTCTTTGCTCTTGATGTTGAGCCCTACCGATACAGTGGTGTTA
  ACATGACAGGGTTCAGAATATTAAATACAGAAAATACCCAAGTCTCCTCCATCATTGAAA
  AGTGGTCGATGGAACGATTGCAGGCACCTCCGAAACCCGATTCAGGTTTGCTGGATGGAT
  TTATGACGACTGATGCTGCTCTAATGTATGATGCTGTGCATGTGGTGTCTGTGGCCGTTC
  AACAGTTTCCCCAGATGACAGTCAGTTCCTTGCAGTGTAATCGACATAAACCCTGGCGCT
  TCGGGACCCGCTTTATGAGTCTAATTAAAGAGGCACATTGGGAAGGCCTCACAGGCAGAA
  TAACTTTCAACAAAACCAATGGCTTGAGAACAGATTTTGATTTGGATGTGATCAGTCTGA
  AGGAAGAAGGTCTAGAAAAGATTGGAACGTGGGATCCAGCCAGTGGCCTGAATATGACAG
  AAAGTCAAAAGGGAAAGCCAGCGAACATCACAGATTCCTTATCCAATCGTTCTTTGATTG
  TTACCACCATTTTGGAAGAGCCTTATGTCCTTTTTAAGAAGTCTGACAAACCTCTCTATG
  GTAATGATCGATTTGAAGGCTATTGCATTGATCTCCTCAGAGAGTTATCTACAATCCTTG
  GCTTTACATATGAAATTAGACTTGTGGAAGATGGGAAATATGGAGCCCAGGATGATGCCA
  ATGGACAATGGAATGGAATGGTTCGTGAACTAATTGATCATAAAGCTGACCTTGCAGTTG
  CTCCACTGGCTATTACCTATGTTCGAGAGAAGGTCATCGACTTTTCCAAGCCCTTTATGA
  CACTTGGAATAAGTATTTTGTACCGCAAGCCCAATGGTACAAACCCAGGCGTCTTCTCCT
  TCCTGAATCCTCTCTCCCCTGATATCTGGATGTATATTCTGCTGGCTTACTTGGGTGTCA
  GTTGTGTGCTCTTTGTCATAGCCAGGTTTAGTCCTTATGAGTGGTATAATCCACACCCTT
  GCAACCCTGACTCAGACGTGGTGGAAAACAATTTTACCTTGCTAAATAGTTTCTGGTTTG
  GAGTTGGAGCTCTCATGCAGCAAGGTTCTGAGCTCATGCCCAAAGCACTGTCCACCAGGA
  TAGTGGGAGGCATTTGGTGGTTTTTCACACTTATCATCATTTCTTCGTATACTGCTAACT
  TAGCCGCCTTTCTGACAGTGGAACGCATGGAATCCCCTATTGACTCTGCTGATGATTTAG
  CTAAACAAACCAAGATAGAATATGGAGCAGTAGAGGATGGTGCAACCATGACTTTTTTCA
  AGAAATCAAAAATCTCCACGTATGACAAAATGTGGGCCTTTATGAGTAGCAGAAGGCAGT
  CAGTGCTGGTCAAAAGTAATGAAGAAGGAATCCAGCGAGTCCTCACCTCTGATTATGCTT
  TCCTAATGGAGTCAACAACCATCGAGTTTGTTACCCAGCGGAACTGTAACCTGACACAGA
  TTGGCGGCCTTATAGACTCTAAAGGTTATGGCGTTGGCACTCCCATGGGTTCTCCATATC
  GAGACAAAATTACCATAGCAATTCTTCAGCTGCAAGAGGAAGGCAAACTGCATATGATGA
  AGGAGAAATGGTGGAGGGGCAATGGTTGCCCAGAAGAGGAGAGCAAAGAGGCCAGTGCCC
  TGGGGGTTCAGAATATTGGTGGCATCTTCATTGTTCTGGCAGCCGGCTTGGTGCTTTCAG
  TTTTTGTGGCAGTGGGAGAATTTTTATACAAATCCAAAAAAAACGCTCAATTGGAAAAGG
  AATCTTCTATTTGGTTAGTGCCACCATACCATCCAGACACTGTTTAGT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175768 unedited
  CGTCACAATTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGATTTCTC
  CCGGATGCTCTCCGACTAACATGGATGTCCCACCATTCCTTGCAGTGGAAGGTTGTTCCT
  TGGCGCAGTGAGTGAAGAACATGCAGCGATTGCTAATGGGTTTGGGAAGCGGAGACTCCT
  TCCTCTCTCTATGACCATGCCGTGATCGTGTCTGCGGTCACCACTCGACGCATCCTCATT
  TCTACCCGAACCCAGGAGCCGAACGCTAGATCGGGGAAGTGGGTGCCGTGCGTGTGGGCA
  CAGAAACACCATGAAGATTATTTTCCCGATTCTAAGTAATCCAGTCTTCAGGCGCACCGT
  TAAACTCCTGCTCTGTTTACTGTGGATTGGATATTCTCAAGGAACCACACATGTATTAAG
  ATTTGGTGGTATTTTTGAATATGTGGAATCTGGCCCAATGGGAGCTGAGGAACTTGCATT
  CAGATTTGCTGTGAACACAATTAACAGAAACAGAACATTGCTACCCAATACTACCCTTAC
  CTATGATACCCAGAAGATAAACCTTTATGATAGTTTTGAAGCATCCAAGAAAGCCTGTGA
  TCAGCTGTCTCTTGGGGTGGCTGCCATCTTNCGGCCTTCACACAGCTCATCAGCAAACGC
  AGTGCAGTCCATCTGCAATGCTCTGGGAGTTCCCCACATACAGACCCGCTGGAAGCACCA
  GGTGTCAGACAACAAAAGATCCTTCTATGTCAGTCTCTACCCAGACTTTTTTTCACTCAG
  CCGTGCCATTTTAGAACTGGTGCAGTTTTTTCAAGTGGAAAACCGTACGGTTGGGTATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_175768 unedited
  NCCCTGGGTGATGGCACTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGATGCCA
  CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACTAAACAG
  TGTCTGGATGGTATGGTGGCACTAACCAAATAGAAGATTCCTTTTCCAATTGAGCGTTTT
  TTTTGGATTTGTATAAAAATTCTCCCACTGCCACAAAAACTGAAAGCACCAAGCCGGCTG
  CCAGAACAATGAAGATGCCACCAATATTCTGAACCCCCAGGGCACTGGCCTCTTTGCTCT
  CCTCTTCTGGGCAACCATTGCCCCTCCACCATTTCTCCTTCATCATATGCAGTTTGCCTT
  CCTCTTGCAGCTGAAGAATTGCTATGGTAATTTTGTCTCGATATGGAGAACCCATGGGAG
  TGCCAACGCCATAACCTTTAGAGTCTATAAGGCCGCCAATCTGTGTCAGGTTACAGTTCC
  GCTGGGTAACAAACTCGATGGTTGTTGACTCCATTAGGAAAGCATAATCAGAGGTGAGGA
  CTCGCTGGATTCCTTCTTCATTACTTTTGACCAGCACTGACTGCCTTCTGCTACTCATAA
  AGGCCCACATTTTGTCATACGTGGAGATTTTTGATTTCTTGAAAAAAGTCATGGTTGCAC
  CATCCTCTACTGCTCCATATTCTATCTTGGTTTGTTTAGCTAAATCATCAGCAGAGTCAA
  TAGGGGATTCCATGCGTTCCACTGTCAGAAAAGGCGCTAAGTTAGCAGTATACGAAGAAA
  TGATGATNAGTGTGAAAAACCACAAATGCCTCCNACTATNCTGNTGNACAGTGCTTTTGG
  CATGAGCTCAGAACCTTGCTGCATGAGAGCTCCCACTCCCAACCAGAACTATTTAGCAAG
  
• Restriction Sites: Please inquire
• ACCN: NM_175768
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175768.1, NP_786944.1
• RefSeq Size: 3409 bp
• RefSeq ORF: 2610 bp
• Locus ID: 2898
• UniProt ID: Q13002
• Cytogenetics: 6q16.3
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive cognitive disability. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) contains an additional exon in the 3' coding region, compared to transcript variant 1. The resulting isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. RNA editing changes Ile567Val, Tyr571Cys and Gln621Arg. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.