SLC22A14 (NM_004803) Human Tagged ORF Clone

CAT#: RC223019

SLC22A14 (Myc-DDK-tagged)-Human solute carrier family 22, member 14 (SLC22A14)

Product Images

Circular map for RC223019

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC22A14
• Synonyms: OCTL2; OCTL4; ORCTL4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223019 representing NM_004803
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGCAGGAGAGGAGAACTTCAAGGAAGAGCTCAGATCCCAGGATGCTTCCAGGAACTTGAACCAGCATG
  AGGTAGCAGGACATCCACATTCCTGGTCTCTGGAGATGCTGTTACGCAGATTGAGGGCTGTCCACACCAA
  GCAGGATGACAAGTTTGCCAACCTCCTGGATGCGGTGGGGGAGTTTGGCACATTCCAGCAGAGGCTAGTA
  GCCCTCACCTTTATCCCCAGCATCATGTCGGCCTTCTTCATGTTTGCTGACCACTTCGTGTTCACAGCCC
  AGAAGCCCTATTGCAATACCAGCTGGATCCTGGCAGTGGGCCCCCACCTGTCCAAAGCTGAGCAGCTGAA
  TCTGACCATACCCCAAGCACCCAATGGCAGTTTCCTGACATGCTTCATGTACCTTCCTGTGCCTTGGAAT
  CTGGATTCTACCATCCAGTTTGGCCTCAATGACACAGACACATGCCAAGATGGGTGGATCTATCCTGACG
  CTAAGAAGCGATCGCTGATCAATGAGTTTGACTTGGTATGTGGCATGGAGACGAAGAAGGACACTGCACA
  GATCATGTTCATGGCAGGGCTCCCGATAGGCTCTCTCATCTTCAGGCTCATAACTGACAAGATGGGCCGC
  TACCCTGCCATCCTGCTGTCACTGCTGGGGCTGATCATCTTCGGCTTTGGGACAGCCTTCATGAACAGCT
  TTCACCTGTATTTGTTCTTTCGCTTTGGCATCTCGCAGTCAGTGGTGGGCTACGCCATCAGCAGCATTTC
  TTTGGCCACTGAGTGGTTAGTGGGTGAGCACCGGGCCCACGCCATTATCCTGGGACACTGCTTTTTCGCT
  GTGTGGGCCATGTTGCTGACAGGGATCGCCTACGGTCTTCCCCACTGGCAGCTGCTGTTTCTGGTGGGTG
  GGATACTTGTGATCCCCTTCATCTCCTATATCTGGATTCTCCCGGAGTCCCCGCGGTGGCTGATGATGAA
  AGGGAAGGTGAAGGAGGCCAAGCAGGTGCTGTGCTACGCCGCAAGTGTGAACAAGAAGACCATTCCTTCA
  AATCTGCTGGACGAGCTGCAGCTGCCCAGAAAGAAGGTGACTCGGGCCTCTGTCCTGGACTTCTGTAAGA
  ATAGGCAGCTCTGCAAGGTGACCTTGGTGATGAGCTGTGTGTGGTTTACCGTCAGTTACACCTATTTTAC
  GTTGAGCCTGAGAATGAGAGAGCTGGGCGTGAGCGTCCACTTCAGACACGTGGTCCCCAGCATCATGGAG
  GTGCCTGCCCGGCTGTGCTGCATCTTTCTCCTCCAGCAGATTGGGAGGAAGTGGAGCCTGGCTGTGACTC
  TCCTCCAAGCCATCATCTGGTGCTTGCTTCTCCTTTTCCTCCCTGAAGGGGAGGATGGCCTCAGACTCAA
  GTGGCCACGTTGTCCGGCCACAGAGCTGAAATCCATGACGATCTTGGTGCTCATGCTCAGAGAGTTCAGC
  CTGGCCGCCACTGTCACTGTGTTCTTCCTCTACACCGCTGAGCTCCTCCCCACTGTGCTCAGGGCGACAG
  GTCTGGGGCTGGTGTCTCTGGCCTCGGTGGCTGGAGCCATCTTGTCCCTGACAATCATCAGCCAGACCCC
  CTCCCTCCTGCCCATCTTTCTCTGCTGCGTCTTAGCCATCGTGGCCTTTTCCCTCTCCTCCCTGCTGCCG
  GAAACGCGAGATCAGCCCCTCTCCGAGAGCCTGAACCACTCCTCACAGATAAGGAATAAGGTCAAGGACA
  TGAAGACTAAGGAAACATCATCTGATGATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223019 representing NM_004803
  Red=Cloning site Green=Tags(s)
  
  MAGEENFKEELRSQDASRNLNQHEVAGHPHSWSLEMLLRRLRAVHTKQDDKFANLLDAVGEFGTFQQRLV
  ALTFIPSIMSAFFMFADHFVFTAQKPYCNTSWILAVGPHLSKAEQLNLTIPQAPNGSFLTCFMYLPVPWN
  LDSTIQFGLNDTDTCQDGWIYPDAKKRSLINEFDLVCGMETKKDTAQIMFMAGLPIGSLIFRLITDKMGR
  YPAILLSLLGLIIFGFGTAFMNSFHLYLFFRFGISQSVVGYAISSISLATEWLVGEHRAHAIILGHCFFA
  VWAMLLTGIAYGLPHWQLLFLVGGILVIPFISYIWILPESPRWLMMKGKVKEAKQVLCYAASVNKKTIPS
  NLLDELQLPRKKVTRASVLDFCKNRQLCKVTLVMSCVWFTVSYTYFTLSLRMRELGVSVHFRHVVPSIME
  VPARLCCIFLLQQIGRKWSLAVTLLQAIIWCLLLLFLPEGEDGLRLKWPRCPATELKSMTILVLMLREFS
  LAATVTVFFLYTAELLPTVLRATGLGLVSLASVAGAILSLTIISQTPSLLPIFLCCVLAIVAFSLSSLLP
  ETRDQPLSESLNHSSQIRNKVKDMKTKETSSDDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004803
• ORF Size: 1782 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004803.2, NP_004794.1
• RefSeq Size: 2201 bp
• RefSeq ORF: 1785 bp
• Locus ID: 9389
• UniProt ID: Q9Y267
• Cytogenetics: 3p22.2
• Protein Families: Transmembrane
• MW: 66.5 kDa
• Gene Summary: This gene encodes a member of the organic-cation transporter family. It is located in a gene cluster with another member of the family, organic cation transporter like 3. The encoded protein is a transmembrane protein which is thought to transport small molecules and since this protein is conserved among several species, it is suggested to have a fundamental role in mammalian systems. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]