MYO6 (NM_004999) Human Tagged ORF Clone

CAT#: RC222917

MYO6 (Myc-DDK-tagged)-Human myosin VI (MYO6)

Product Images

Circular map for RC222917

• 

  Coomassie blue staining of purified MYO6 protein (Cat# TP322917). The protein was produced from HEK293T cells transfected with MYO6 cDNA clone (Cat# RC222917) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MYO6
• Synonyms: DFNA22; DFNB37
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222917 representing NM_004999
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGATGGAAAGCCCGTTTGGGCGCCACACCCTACAGATGGATTTCAGATGGGCAATATTGTGGATA
  TTGGCCCCGACAGCTTAACAATTGAACCCTTGAATCAGAAAGGCAAGACATTTTTGGCTCTCATAAACCA
  AGTGTTTCCTGCAGAAGAGGACAGTAAAAAAGATGTGGAAGATAACTGTTCACTAATGTATTTAAATGAA
  GCCACACTGCTCCATAATATCAAAGTTCGATATAGTAAAGACAGAATTTATACATATGTCGCCAACATTC
  TGATTGCAGTGAATCCATACTTTGACATACCTAAAATATATTCTTCAGAAGCAATAAAGTCATATCAAGG
  AAAATCTCTTGGGACAAGACCACCTCATGTCTTTGCAATTGCTGATAAAGCTTTTCGAGACATGAAGGTG
  CTCAAGATGAGTCAGTCTATCATTGTATCTGGAGAATCAGGAGCCGGCAAAACAGAAAATACAAAATTTG
  TTCTAAGATACCTGACTGAATCCTATGGAACAGGTCAAGATATTGATGACAGAATTGTTGAAGCTAACCC
  ACTCCTAGAAGCCTTTGGAAATGCGAAGACTGTTCGCAACAATAATAGCAGTCGATTTGGGAAATTTGTA
  GAAATACATTTTAATGAAAAGAGCTCAGTTGTTGGAGGATTTGTTTCACATTATCTCCTAGAGAAATCTA
  GGATCTGTGTTCAAGGCAAAGAGGAAAGAAATTATCATATCTTTTATAGGTTGTGTGCTGGTGCTTCTGA
  AGATATTAGAGAAAAACTTCATTTGAGTTCACCAGATAATTTTCGGTATTTAAACCGAGGCTGCACTAGA
  TACTTTGCTAACAAAGAAACTGACAAACAGATTTTACAGAACCGCAAAAGTCCTGAGTACCTTAAGGCAG
  GTTCTATGAAAGATCCTCTGCTAGATGACCATGGTGATTTTATTAGAATGTGCACGGCTATGAAAAAAAT
  TGGTTTGGATGATGAAGAAAAGCTTGATCTCTTCCGGGTAGTAGCTGGCGTCCTGCACCTTGGAAATATT
  GATTTTGAGGAAGCTGGCAGCACTTCAGGTGGTTGTAATCTGAAGAATAAATCTGCTCAGTCTTTGGAAT
  ATTGTGCTGAATTACTGGGTTTGGACCAAGATGATCTTCGAGTAAGTTTGACCACAAGAGTCATGCTAAC
  AACAGCAGGGGGCACCAAAGGAACAGTTATAAAGGTACCTCTGAAAGTGGAGCAAGCAAACAATGCTCGT
  GATGCCCTGGCAAAGACAGTGTATAGCCATCTTTTTGATCATGTGGTAAACAGAGTAAATCAGTGTTTTC
  CTTTTGAAACATCATCCTATTTTATTGGAGTCCTAGATATTGCTGGTTTTGAGTACTTTGAGCATAACAG
  TTTTGAACAATTTTGCATCAACTATTGCAATGAAAAACTTCAACAATTTTTTAATGAAAGGATTCTGAAG
  GAGGAACAAGAACTCTATCAAAAAGAAGGTTTAGGTGTTAATGAAGTGCATTATGTGGATAATCAGGACT
  GTATAGATTTAATTGAAGCCAAATTAGTGGGAATACTGGATATTTTGGATGAAGAAAATCGCCTTCCCCA
  GCCAAGTGATCAACACTTTACATCTGCAGTTCACCAAAAGCACAAGGATCATTTTCGACTCACTATTCCC
  AGAAAATCTAAGCTGGCAGTTCATAGGAATATCAGAGACGACGAAGGCTTCATTATCAGGCATTTTGCGG
  GGGCAGTGTGCTATGAAACAACCCAGTTTGTGGAGAAAAATAATGATGCTTTACATATGTCTCTTGAATC
  CTTAATATGTGAATCCAGAGATAAGTTTATACGGGAATTATTTGAATCATCCACAAATAACAACAAAGAT
  ACTAAACAAAAAGCAGGAAAACTTAGCTTCATCAGCGTGGGAAACAAGTTTAAGACACAGTTAAATTTGC
  TTCTGGATAAACTTCGAAGTACTGGAGCAAGCTTTATTCGTTGCATCAAACCTAACTTAAAGATGACAAG
  CCACCACTTTGAAGGTGCTCAAATTCTGTCTCAGCTTCAGTGTTCAGGGATGGTGTCTGTTTTGGACTTG
  ATGCAGGGTGGTTACCCATCACGAGCTTCATTTCATGAACTCTACAACATGTACAAAAAGTATATGCCAG
  ATAAACTTGCAAGATTGGATCCAAGACTATTTTGTAAGGCTTTGTTTAAAGCTTTGGGCTTAAATGAAAA
  TGACTACAAGTTTGGGTTAACCAAAGTATTTTTTAGACCTGGCAAGTTTGCAGAATTTGATCAGATCATG
  AAGTCTGACCCTGACCACTTAGCAGAGTTGGTTAAAAGAGTCAATCACTGGCTCACATGCAGTCGCTGGA
  AGAAAGTTCAGTGGTGCTCACTCTCAGTCATCAAATTGAAAAACAAAATAAAATATCGAGCTGAAGCCTG
  CATTAAAATGCAAAAAACTATTCGAATGTGGCTTTGCAAGAGGAGACACAAACCTCGCATTGATGGTCTG
  GTTAAGGTGGGCACACTGAAAAAACGACTTGATAAATTTAATGAGGTAGTCAGTGTGTTGAAAGATGGAA
  AACCCGAGATGAATAAACAGATCAAGAATCTGGAAATTTCTATTGATACTTTGATGGCCAAAATTAAGTC
  CACTATGATGACGCAGGAACAAATCCAGAAAGAATATGATGCACTGGTTAAAAGCTCAGAGGAACTCCTC
  AGTGCATTACAGAAAAAAAAACAGCAGGAAGAGGAAGCAGAAAGGCTGAGGCGTATTCAAGAAGAAATGG
  AAAAGGAAAGAAAAAGACGTGAAGAAGACGAAAAACGTCGAAGAAAGGAAGAGGAGGAAAGGCGGATGAA
  ACTTGAGATGGAAGCAAAGAGAAAACAAGAAGAAGAAGAGAGAAAGAAAAGGGAAGATGATGAAAAACGC
  ATTCAAGCTGAAGTGGAGGCACAGCTGGCCCGACAGAAGGAGGAGGAATCCCAACAGCAAGCAGTTCTGG
  AGCAGGAGCGCAGGGACCGGGAGCTGGCCCTGAGGATTGCCCAGAGTGAAGCCGAGCTCATCAGTGATGA
  GGCCCAGGCCGACCTGGCGCTGCGGAGAAATGATGGAACAAGACCCAAAATGACACCGGAACAAATGGCC
  AAAGAAATGTCAGAATTTTTGAGTAGAGGTCCTGCTGTACTAGCCACCAAAGCAGCTGCTGGTACTAAGA
  AATATGATCTTAGTAAATGGAAATATGCAGAACTACGTGATACCATCAATACTTCTTGTGATATTGAGCT
  CCTGGCAGCTTGCAGAGAAGAATTTCATAGGAGACTAAAAGTGTATCATGCTTGGAAATCTAAGAACAAG
  AAGAGAAATACTGAAACAGAGCAACGTGCTCCAAAGTCTGTTACTGATTATGATTTTGCACCATTTTTGA
  ACAATTCACCTCAGCAAAACCCAGCAGCTCAGATTCCTGCCAGGCAGCGGGAGATTGAAATGAACCGACA
  GCAACGCTTCTTCCGCATCCCATTCATCCGCCCTGCCGACCAGTACAAAGACCCTCAGAGTAAGAAAAAA
  GGCTGGTGGTATGCCCATTTTGATGGACCATGGATTGCCCGGCAAATGGAACTCCATCCTGACAAGCCAC
  CCATCCTACTTGTGGCTGGTAAGGACGACATGGAGATGTGTGAGCTGAATCTTGAGGAGACTGGCCTGAC
  TCGGAAGCGTGGTGCTGAGATCTTGCCAAGACAGTTTGAAGAAATCTGGGAACGCTGTGGAGGCATCCAG
  TACCTTCAGAATGCGATTGAGAGCAGACAGGCTCGGCCCACCTATGCAACAGCCATGCTGCAGAGTCTGT
  TAAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222917 representing NM_004999
  Red=Cloning site Green=Tags(s)
  
  MEDGKPVWAPHPTDGFQMGNIVDIGPDSLTIEPLNQKGKTFLALINQVFPAEEDSKKDVEDNCSLMYLNE
  ATLLHNIKVRYSKDRIYTYVANILIAVNPYFDIPKIYSSEAIKSYQGKSLGTRPPHVFAIADKAFRDMKV
  LKMSQSIIVSGESGAGKTENTKFVLRYLTESYGTGQDIDDRIVEANPLLEAFGNAKTVRNNNSSRFGKFV
  EIHFNEKSSVVGGFVSHYLLEKSRICVQGKEERNYHIFYRLCAGASEDIREKLHLSSPDNFRYLNRGCTR
  YFANKETDKQILQNRKSPEYLKAGSMKDPLLDDHGDFIRMCTAMKKIGLDDEEKLDLFRVVAGVLHLGNI
  DFEEAGSTSGGCNLKNKSAQSLEYCAELLGLDQDDLRVSLTTRVMLTTAGGTKGTVIKVPLKVEQANNAR
  DALAKTVYSHLFDHVVNRVNQCFPFETSSYFIGVLDIAGFEYFEHNSFEQFCINYCNEKLQQFFNERILK
  EEQELYQKEGLGVNEVHYVDNQDCIDLIEAKLVGILDILDEENRLPQPSDQHFTSAVHQKHKDHFRLTIP
  RKSKLAVHRNIRDDEGFIIRHFAGAVCYETTQFVEKNNDALHMSLESLICESRDKFIRELFESSTNNNKD
  TKQKAGKLSFISVGNKFKTQLNLLLDKLRSTGASFIRCIKPNLKMTSHHFEGAQILSQLQCSGMVSVLDL
  MQGGYPSRASFHELYNMYKKYMPDKLARLDPRLFCKALFKALGLNENDYKFGLTKVFFRPGKFAEFDQIM
  KSDPDHLAELVKRVNHWLTCSRWKKVQWCSLSVIKLKNKIKYRAEACIKMQKTIRMWLCKRRHKPRIDGL
  VKVGTLKKRLDKFNEVVSVLKDGKPEMNKQIKNLEISIDTLMAKIKSTMMTQEQIQKEYDALVKSSEELL
  SALQKKKQQEEEAERLRRIQEEMEKERKRREEDEKRRRKEEEERRMKLEMEAKRKQEEEERKKREDDEKR
  IQAEVEAQLARQKEEESQQQAVLEQERRDRELALRIAQSEAELISDEAQADLALRRNDGTRPKMTPEQMA
  KEMSEFLSRGPAVLATKAAAGTKKYDLSKWKYAELRDTINTSCDIELLAACREEFHRRLKVYHAWKSKNK
  KRNTETEQRAPKSVTDYDFAPFLNNSPQQNPAAQIPARQREIEMNRQQRFFRIPFIRPADQYKDPQSKKK
  GWWYAHFDGPWIARQMELHPDKPPILLVAGKDDMEMCELNLEETGLTRKRGAEILPRQFEEIWERCGGIQ
  YLQNAIESRQARPTYATAMLQSLLK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004999
• ORF Size: 3855 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004999.4
• RefSeq Size: 8662 bp
• RefSeq ORF: 3858 bp
• Locus ID: 4646
• UniProt ID: Q9UM54
• Cytogenetics: 6q14.1
• Domains: IQ, myosin_head
• MW: 149.2 kDa
• Gene Summary: This gene encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. The protein consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. This protein maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]