EDC4 (NM_014329) Human Tagged ORF Clone

CAT#: RC222770

EDC4 (Myc-DDK-tagged)-Human enhancer of mRNA decapping 4 (EDC4)

Product Images

Circular map for RC222770

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EDC4
• Synonyms: Ge-1; GE1; HEDL5; HEDLS; RCD-8; RCD8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222770 representing NM_014329
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCTGCGCGAGCATCGACATCGAGGACGCCACGCAGCACCTGCGGGACATCCTCAAGCTGGACC
  GGCCCGCGGGCGGCCCCAGTGCAGAGAGCCCACGGCCATCCAGTGCCTACAATGGGGACCTCAATGGACT
  TCTGGTCCCAGACCCGCTCTGCTCAGGTGATAGTACCTCAGCAAACAAGACTGGTCTTCGGACCATGCCA
  CCCATTAACCTGCAAGAGAAGCAGGTCATCTGTCTCTCAGGAGATGATAGCTCCACCTGCATTGGGATTT
  TGGCCAAGGAGGTGGAGATTGTGGCTAGCAGTGACTCTAGCATTTCAAGCAAGGCCCGGGGAAGCAACAA
  GGTGAAAATTCAGCCTGTCGCCAAGTATGACTGGGAACAGAAGTACTACTATGGCAACCTGATTGCTGTG
  TCTAACTCCTTCTTGGCCTATGCCATTCGGGCTGCCAACAATGGCTCTGCCATGGTGCGGGTGATCAGCG
  TCAGCACTTCGGAGCGGACCTTGCTCAAGGGCTTCACAGGCAGTGTGGCTGATCTGGCTTTCGCGCACCT
  CAACTCTCCACAGCTGGCCTGCCTGGATGAGGCAGGCAACCTGTTCGTGTGGCGCTTGGCTCTGGTTAAT
  GGCAAAATTCAAGAAGAGATCTTGGTCCATATTCGGCAGCCAGAGGGCACGCCACTGAACCACTTTCGCA
  GGATCATCTGGTGCCCCTTCATCCCTGAGGAGAGCGAAGACTGCTGTGAGGAGAGCAGCCCAACAGTGGC
  CCTGCTGCATGAAGACCGGGCTGAGGTGTGGGACCTGGACATGCTCCGCTCCAGCCACAGTACCTGGCCT
  GTGGATGTTAGCCAGATCAAGCAGGGCTTCATTGTGGTAAAAGGTCATAGCACGTGCCTCAGTGAAGGAG
  CCCTCTCTCCTGATGGGACTGTGCTGGCTACTGCGAGCCACGATGGCTATGTCAAGTTCTGGCAGATCTA
  CATTGAGGGGCAAGATGAGCCAAGGTGTCTGCACGAGTGGAAACCTCATGATGGGCGGCCCCTCTCCTGC
  CTCCTGTTCTGTGACAACCATAAGAAACAAGACCCTGATGTCCCTTTCTGGAGGTTCCTTATTACTGGTG
  CTGACCAGAACCGAGAGTTAAAGATGTGGTGTACAGTATCCTGGACCTGCCTGCAGACTATTCGCTTCTC
  CCCAGATATCTTCAGCTCAGTGAGTGTGCCCCCTAGCCTCAAGGTTTGCTTGGACCTCTCAGCAGAATAC
  CTGATTCTCAGCGATGTGCAACGGAAGGTCCTCTATGTGATGGAGCTGCTGCAAAACCAGGAGGAGGGCC
  ACGCCTGCTTCAGCTCCATCTCGGAGTTCCTGCTCACCCACCCTGTGCTGAGCTTTGGTATCCAGGTTGT
  GAGTCGCTGCCGGCTACGGCACACTGAGGTGCTGCCTGCCGAAGAGGAAAATGACAGCCTGGGTGCTGAT
  GGTACCCATGGAGCCGGTGCCATGGAGTCTGCGGCCGGTGTGCTCATCAAGCTCTTTTGTGTGCATACTA
  AGGCACTGCAAGATGTGCAGATCCGCTTCCAGCCACAGCTGAACCCTGATGTGGTGGCCCCACTGCCCAC
  CCACACTGCCCACGAGGACTTCACATTTGGAGAGTCTCGGCCCGAACTGGGCTCTGAGGGCCTGGGGTCA
  GCCGCTCACGGCTCCCAGCCTGACCTCCGACGAATCGTGGAGCTGCCTGCACCTGCCGACTTCCTCAGTC
  TGAGCAGTGAGACCAAGCCCAAGTTGATGACACCTGACGCCTTCATGACACCTAGCGCCTCCTTGCAGCA
  GATCACTGCCTCTCCCAGCAGCAGCAGCAGCGGTAGCAGCAGCAGCAGCAGCAGTAGCAGCAGCTCCCTT
  ACAGCTGTGTCTGCCATGAGCAGCACCTCAGCTGTGGACCCCTCCTTGACCAGGCCACCTGAGGAGCTGA
  CCTTGAGCCCCAAGCTGCAGCTGGATGGCAGCCTGACAATGAGCAGCAGTGGCAGCCTTCAGGCAAGCCC
  GCGTGGCCTCCTGCCTGGCCTGCTCCCAGCCCCAGCTGACAAACTGACTCCCAAGGGGCCGGGCCAGGTG
  CCTACTGCCACCTCTGCACTGTCCCTGGAGCTGCAGGAAGTGGAGCCCCTGGGGCTACCCCAAGCCTCCC
  CTAGCCGCACTCGTTCCCCTGATGTCATCTCCTCAGCTTCCACTGCCCTGTCCCAGGACATCCCTGAGAT
  TGCATCTGAGGCCCTGTCCCGTGGTTTTGGCTCCTCTGCACCAGAGGGCCTTGAGCCAGACAGTATGGCT
  TCAGCCGCCTCGGCACTGCACCTGCTGTCCCCACGGCCCCGGCCAGGGCCCGAGCTCGGCCCCCAGCTCG
  GGCTTGATGGAGGCCCTGGGGATGGAGATCGGCATAATACCCCCTCCCTCCTGGAGGCAGCCTTGACCCA
  GGAGGCCTCGACTCCTGACAGTCAGGTTTGGCCCACAGCACCTGACATTACTCGTGAGACCTGCAGCACC
  CTGGCAGAAAGCCCCAGGAATGGCCTTCAGGAAAAGCACAAGAGCCTGGCCTTCCACCGACCACCATATC
  ACCTGCTGCAGCAACGTGACAGCCAGGATGCCAGTGCTGAGCAAAGTGACCATGATGATGAGGTGGCCAG
  CCTTGCCTCTGCTTCAGGAGGCTTTGGCACCAAAGTTCCTGCTCCACGGCTGCCTGCCAAGGACTGGAAG
  ACCAAGGGATCCCCTCGAACCTCACCCAAGCTCAAGAGGAAAAGCAAGAAGGATGATGGGGATGCAGCCA
  TGGGATCCCGGCTCACAGAGCACCAGGTGGCAGAGCCCCCTGAGGACTGGCCAGCACTAATTTGGCAACA
  GCAGAGAGAGCTGGCAGAGCTGCGGCACAGCCAGGAAGAGCTGCTGCAGCGTCTGTGTACCCAACTCGAA
  GGCCTGCAGAGCACAGTCACAGGCCACGTAGAACGTGCCCTTGAGACTCGGCACGAGCAGGAACAGCGGC
  GGCTGGAGCGAGCACTGGCTGAGGGGCAGCAGCGGGGAGGGCAGCTGCAGGAGCAGCTGACACAACAGTT
  GTCCCAAGCACTGTCGTCAGCTGTAGCTGGGCGGCTAGAGCGCAGCATACGGGATGAGATCAAGAAGACA
  GTCCCTCCATGTGTCTCAAGGAGTCTGGAGCCTATGGCAGGCCAACTGAGCAACTCAGTGGCTACCAAGC
  TCACAGCTGTGGAGGGCAGCATGAAAGAGAACATCTCCAAGCTGCTCAAGTCCAAGAACTTGACTGATGC
  CATCGCCCGAGCAGCTGCAGACACATTACAAGGGCCGATGCAGGCTGCCTACCGGGAAGCCTTCCAGAGT
  GTGGTGCTGCCGGCCTTTGAGAAGAGCTGCCAGGCCATGTTCCAGCAAATCAATGATAGCTTCCGGCTGG
  GGACACAGGAATACTTGCAGCAGCTAGAAAGCCACATGAAGAGCCGGAAGGCACGGGAACAGGAGGCCAG
  GGAGCCTGTGCTAGCCCAGCTGCGGGGCCTGGTCAGCACACTGCAGAGTGCCACTGAGCAGATGGCAGCC
  ACCGTGGCCGGCAGTGTTCGTGCTGAGGTGCAGCACCAGCTGCATGTGGCTGTGGGCAGCCTGCAGGAGT
  CCATTTTAGCACAGGTACAGCGCATCGTTAAGGGTGAGGTGAGTGTGGCGCTCAAGGAGCAGCAGGCCGC
  CGTCACCTCCAGCATCATGCAGGCCATGCGCTCAGCTGCTGGCACACCTGTCCCCTCTGCCCACCTTGAC
  TGCCAGGCCCAGCAAGCCCATATCCTGCAGCTGCTGCAGCAGGGCCACCTCAATCAGGCCTTCCAGCAGG
  CGCTGACAGCTGCTGACCTGAACCTGGTGCTGTATGTGTGTGAAACTGTGGACCCAGCCCAGGTTTTTGG
  GCAGCCACCCTGCCCGCTCTCCCAGCCTGTGCTCCTTTCCCTCATCCAGCAGCTGGCATCTGACCTTGGC
  ACTCGAACTGACCTCAAGCTCAGCTACCTGGAAGAGGCCGTGATGCACCTGGACCACAGTGACCCCATCA
  CTCGGGACCACATGGGCTCCGTTATGGCCCAGGTGCGCCAAAAGCTTTTTCAGTTCCTGCAGGCTGAGCC
  ACACAACTCACTTGGCAAAGCAGCTCGGCGTCTCAGCCTCATGCTGCATGGCCTCGTGACCCCCAGCCTC
  CCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222770 representing NM_014329
  Red=Cloning site Green=Tags(s)
  
  MASCASIDIEDATQHLRDILKLDRPAGGPSAESPRPSSAYNGDLNGLLVPDPLCSGDSTSANKTGLRTMP
  PINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAV
  SNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVN
  GKIQEEILVHIRQPEGTPLNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHSTWP
  VDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGYVKFWQIYIEGQDEPRCLHEWKPHDGRPLSC
  LLFCDNHKKQDPDVPFWRFLITGADQNRELKMWCTVSWTCLQTIRFSPDIFSSVSVPPSLKVCLDLSAEY
  LILSDVQRKVLYVMELLQNQEEGHACFSSISEFLLTHPVLSFGIQVVSRCRLRHTEVLPAEEENDSLGAD
  GTHGAGAMESAAGVLIKLFCVHTKALQDVQIRFQPQLNPDVVAPLPTHTAHEDFTFGESRPELGSEGLGS
  AAHGSQPDLRRIVELPAPADFLSLSSETKPKLMTPDAFMTPSASLQQITASPSSSSSGSSSSSSSSSSSL
  TAVSAMSSTSAVDPSLTRPPEELTLSPKLQLDGSLTMSSSGSLQASPRGLLPGLLPAPADKLTPKGPGQV
  PTATSALSLELQEVEPLGLPQASPSRTRSPDVISSASTALSQDIPEIASEALSRGFGSSAPEGLEPDSMA
  SAASALHLLSPRPRPGPELGPQLGLDGGPGDGDRHNTPSLLEAALTQEASTPDSQVWPTAPDITRETCST
  LAESPRNGLQEKHKSLAFHRPPYHLLQQRDSQDASAEQSDHDDEVASLASASGGFGTKVPAPRLPAKDWK
  TKGSPRTSPKLKRKSKKDDGDAAMGSRLTEHQVAEPPEDWPALIWQQQRELAELRHSQEELLQRLCTQLE
  GLQSTVTGHVERALETRHEQEQRRLERALAEGQQRGGQLQEQLTQQLSQALSSAVAGRLERSIRDEIKKT
  VPPCVSRSLEPMAGQLSNSVATKLTAVEGSMKENISKLLKSKNLTDAIARAAADTLQGPMQAAYREAFQS
  VVLPAFEKSCQAMFQQINDSFRLGTQEYLQQLESHMKSRKAREQEAREPVLAQLRGLVSTLQSATEQMAA
  TVAGSVRAEVQHQLHVAVGSLQESILAQVQRIVKGEVSVALKEQQAAVTSSIMQAMRSAAGTPVPSAHLD
  CQAQQAHILQLLQQGHLNQAFQQALTAADLNLVLYVCETVDPAQVFGQPPCPLSQPVLLSLIQQLASDLG
  TRTDLKLSYLEEAVMHLDHSDPITRDHMGSVMAQVRQKLFQFLQAEPHNSLGKAARRLSLMLHGLVTPSL
  P
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014329
• ORF Size: 4203 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014329.5
• RefSeq Size: 4811 bp
• RefSeq ORF: 4206 bp
• Locus ID: 23644
• UniProt ID: Q6P2E9
• Cytogenetics: 16q22.1
• Domains: WD40
• Protein Pathways: RNA degradation
• MW: 151.7 kDa
• Gene Summary: In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).[UniProtKB/Swiss-Prot Function]