HIC1 (NM_006497) Human Tagged ORF Clone

CAT#: RC222752

HIC1 (Myc-DDK-tagged)-Human hypermethylated in cancer 1 (HIC1), transcript variant 1

Product Images

Circular map for RC222752

• 

  Coomassie blue staining of purified HIC1 protein (Cat# TP322752). The protein was produced from HEK293T cells transfected with HIC1 cDNA clone (Cat# RC222752) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HIC1
• Synonyms: hic-1; ZBTB29; ZNF901
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222752 representing NM_006497
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGACACGATGGAGGCGCCCGGCCACTCCAGGCAGCTGCTGCTGCAGCTCAACAACCAGCGCACCA
  AGGGCTTCTTGTGCGACGTGATCATCGTGGTGCAGAACGCCCTCTTCCGCGCGCACAAGAACGTGCTGGC
  GGCCAGCAGCGCCTACCTCAAGTCCCTGGTGGTGCATGACAACCTGCTCAACCTGGACCATGACATGGTG
  AGCCCGGCCGTGTTCCGCCTGGTGCTGGACTTCATCTACACCGGCCGCCTGGCTGACGGCGCAGAGGCGG
  CTGCGGCCGCGGCCGTGGCCCCGGGGGCTGAGCCGAGCCTGGGCGCCGTGCTGGCCGCCGCCAGCTACCT
  GCAGATCCCCGACCTCGTGGCGCTGTGCAAGAAACGCCTCAAGCGCCACGGCAAGTACTGCCACCTGCGG
  GGCGGCGGCGGCGGCGGCGGCGGCTACGCGCCCTATGGTCGGCCGGGCCGGGGCCTGCGGGCCGCCACGC
  CGGTCATCCAGGCCTGCTACCCGTCCCCAGTCGGGCCTCCGCCGCCGCCTGCCGCGGAGCCGCCCTCGGG
  CCCAGAGGCCGCGGTCAACACGCACTGCGCCGAGCTGTACGCGTCGGGACCCGGCCCGGCCGCCGCACTC
  TGTGCCTCGGAGCGCCGCTGCTCCCCTCTTTGTGGCCTGGACCTGTCCAAGAAGAGCCCGCCGGGCTCCG
  CGGCGCCAGAGCGGCCGCTGGCTGAGCGCGAGCTGCCCCCGCGCCCGGACAGCCCTCCCAGCGCCGGCCC
  CGCCGCCTACAAGGAGCCGCCTCTCGCCCTGCCGTCGCTGCCGCCGCTGCCCTTCCAGAAGCTGGAGGAG
  GCCGCACCGCCTTCCGACCCATTTCGCGGCGGCAGCGGCAGCCCGGGACCCGAGCCCCCCGGCCGCCCCG
  ACGGGCCTAGTCTCCTCTATCGCTGGATGAAGCACGAGCCGGGCCTGGGTAGCTATGGCGACGAGCTGGG
  CCGGGAGCGCGGCTCCCCCAGCGAGCGCTGCGAAGAGCGTGGTGGGGACGCGGCCGTCTCGCCCGGGGGG
  CCCCCGCTCGGCCTGGCGCCGCCGCCGCGCTACCCTGGCAGCCTGGACGGGCCCGGCGCGGGCGGCGACG
  GCGACGACTACAAGAGCAGCAGCGAGGAGACCGGTAGCAGCGAGGACCCCAGCCCGCCTGGCGGCCACCT
  CGAGGGCTACCCATGCCCGCACCTGGCCTATGGCGAGCCCGAGAGCTTCGGTGACAACCTGTACGTGTGC
  ATTCCGTGCGGCAAGGGCTTCCCCAGCTCTGAGCAGCTGAACGCGCACGTGGAGGCTCACGTGGAGGAGG
  AGGAAGCGCTGTACGGCAGGGCCGAGGCGGCCGAAGTGGCCGCTGGGGCCGCCGGCCTAGGGCCCCCTTT
  TGGAGGCGGCGGGGACAAGGTCGCCGGGGCTCCGGGTGGCCTGGGAGAGCTGCTGCGGCCCTACCGCTGC
  GCGTCGTGCGACAAGAGCTACAAGGACCCGGCCACGCTGCGGCAGCACGAGAAGACGCACTGGCTGACCC
  GGCCCTACCCATGCACCATCTGCGGGAAGAAGTTCACGCAGCGTGGGACCATGACGCGCCACATGCGCAG
  CCACCTGGGCCTCAAGCCCTTCGCGTGCGACGCGTGCGGCATGCGGTTCACGCGCCAGTACCGCCTCACG
  GAGCACATGCGCATCCACTCGGGCGAGAAGCCCTACGAGTGCCAGGTGTGCGGCGGCAAGTTCGCACAGC
  AACGCAACCTCATCAGCCACATGAAGATGCACGCCGTGGGGGGCGCGGCCGGCGCGGCCGGGGCGCTGGC
  GGGCTTGGGGGGGCTCCCCGGCGTCCCCGGCCCCGACGGCAAGGGCAAGCTCGACTTCCCCGAGGGCGTC
  TTTGCTGTGGCTCGCCTCACGGCCGAGCAGCTGAGCCTGAAGCAGCAGGACAAGGCGGCCGCGGCCGAGC
  TGCTGGCGCAGACCACGCACTTCCTGCACGACCCCAAGGTGGCGCTGGAGAGCCTCTACCCGCTGGCCAA
  GTTCACGGCCGAGCTGGGCCTCAGCCCCGACAAGGCGGCCGAGGTGCTGAGCCAGGGCGCTCACCTGGCG
  GCCGGGCCCGACGGCCGGACCATCGACCGTTTCTCTCCCACC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222752 representing NM_006497
  Red=Cloning site Green=Tags(s)
  
  MLDTMEAPGHSRQLLLQLNNQRTKGFLCDVIIVVQNALFRAHKNVLAASSAYLKSLVVHDNLLNLDHDMV
  SPAVFRLVLDFIYTGRLADGAEAAAAAAVAPGAEPSLGAVLAAASYLQIPDLVALCKKRLKRHGKYCHLR
  GGGGGGGGYAPYGRPGRGLRAATPVIQACYPSPVGPPPPPAAEPPSGPEAAVNTHCAELYASGPGPAAAL
  CASERRCSPLCGLDLSKKSPPGSAAPERPLAERELPPRPDSPPSAGPAAYKEPPLALPSLPPLPFQKLEE
  AAPPSDPFRGGSGSPGPEPPGRPDGPSLLYRWMKHEPGLGSYGDELGRERGSPSERCEERGGDAAVSPGG
  PPLGLAPPPRYPGSLDGPGAGGDGDDYKSSSEETGSSEDPSPPGGHLEGYPCPHLAYGEPESFGDNLYVC
  IPCGKGFPSSEQLNAHVEAHVEEEEALYGRAEAAEVAAGAAGLGPPFGGGGDKVAGAPGGLGELLRPYRC
  ASCDKSYKDPATLRQHEKTHWLTRPYPCTICGKKFTQRGTMTRHMRSHLGLKPFACDACGMRFTRQYRLT
  EHMRIHSGEKPYECQVCGGKFAQQRNLISHMKMHAVGGAAGAAGALAGLGGLPGVPGPDGKGKLDFPEGV
  FAVARLTAEQLSLKQQDKAAAAELLAQTTHFLHDPKVALESLYPLAKFTAELGLSPDKAAEVLSQGAHLA
  AGPDGRTIDRFSPT
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006497
• ORF Size: 2142 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006497.4
• RefSeq Size: 2626 bp
• RefSeq ORF: 2145 bp
• Locus ID: 3090
• UniProt ID: Q14526
• Cytogenetics: 17p13.3
• MW: 74.3 kDa
• Gene Summary: This gene functions as a growth regulatory and tumor repressor gene. Hypermethylation or deletion of the region of this gene have been associated with tumors and the contiguous-gene syndrome, Miller-Dieker syndrome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2010]