RCCD1 (NM_001017919) Human Tagged ORF Clone

CAT#: RC222746

RCCD1 (Myc-DDK-tagged)-Human RCC1 domain containing 1 (RCCD1), transcript variant 2

Product Images

Circular map for RC222746

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  Western blot validation of overexpression lysate (Cat# LY422749) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222746 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RCCD1 protein (Cat# TP322746). The protein was produced from HEK293T cells transfected with RCCD1 cDNA clone (Cat# RC222746) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RCCD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222746 representing NM_001017919
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGAGCGGCCGGGGGCCTGGTTCGGCTTCGGTTTCTGCGGCTTCGGGCAGGAGCTGGGCTCCG
  GACGCGGGCGCCAGGTGCACAGCCCCAGTCCGCTGCGGGCGGGCGTCGACATCTGCCGCGTGAGCGCGAG
  CTGGAGCTACACCGCTTTCGTGACCCGTGGAGGCCGCTTGGAGCTGTCGGGCTCAGCCAGCGGCGCGGCG
  GGCCGCTGCAAGGACGCGTGGGCCTCGGAGGGGCTCCTCGCGGTGCTGCGCGCCGGGCCGGGGCCGGAGG
  CGTTACTGCAGGTCTGGGCGGCCGAATCGGCGCTGCGTGGGGAGCCATTGTGGGCCCAGAATGTGGTGCC
  CGAGGCCGAAGGGGAAGACGATCCGGCCGGTGAGGCCCAGGCTGGGAGGCTACCCCTGCTGCCCTGCGCC
  CGTGCCTACGTGAGCCCGCGGGCGCCCTTCTACCGGCCTCTGGCTCCGGAGCTGCGGGCACGCCAGCTGG
  AGCTGGGCGCCGAGCACGCGTTGCTGCTGGACGCTGCTGGCCAGGTGTTCTCCTGGGGCGGGGGCAGGCA
  TGGACAGCTGGGCCATGGGACCCTGGAGGCAGAGCTGGAGCCACGGCTGTTGGAGGCGTTGCAGGGCCTA
  GTCATGGCTGAGGTGGCCGCGGGGGGCTGGCATTCTGTGTGTGTGAGTGAGACTGGGGATATTTATATCT
  GGGGCTGGAATGAATCAGGGCAGCTGGCCCTGCCCACCAGGAACCTGGCAGAGGATGGAGAGACTGTCGC
  AAGGGAAGCCACAGAACTGAATGAAGATGGTTCTCAGGTGAAGAGAACGGGTGGGGCTGAGGATGGAGCC
  CCTGCCCCCTTCATAGCTGTCCAGCCCTTCCCGGCATTACTGGATCTCCCCATGGGCTCAGATGCAGTCA
  AGGCCAGCTGTGGATCCCGGCACACAGCTGTGGTGACACGAACAGGGGAGCTCTACACCTGGGGCTGGGG
  TAAATATGGACAGCTGGGCCACGAGGACACCACCAGCTTGGATCGGCCTCGCCGTGTGGAATACTTTGTA
  GATAAGCAACTCCAAGTAAAGGCTGTCACCTGTGGGCCGTGGAACACCTACGTGTATGCTGTGGAGAAAG
  GGAAGAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222746 representing NM_001017919
  Red=Cloning site Green=Tags(s)
  
  MAEERPGAWFGFGFCGFGQELGSGRGRQVHSPSPLRAGVDICRVSASWSYTAFVTRGGRLELSGSASGAA
  GRCKDAWASEGLLAVLRAGPGPEALLQVWAAESALRGEPLWAQNVVPEAEGEDDPAGEAQAGRLPLLPCA
  RAYVSPRAPFYRPLAPELRARQLELGAEHALLLDAAGQVFSWGGGRHGQLGHGTLEAELEPRLLEALQGL
  VMAEVAAGGWHSVCVSETGDIYIWGWNESGQLALPTRNLAEDGETVAREATELNEDGSQVKRTGGAEDGA
  PAPFIAVQPFPALLDLPMGSDAVKASCGSRHTAVVTRTGELYTWGWGKYGQLGHEDTTSLDRPRRVEYFV
  DKQLQVKAVTCGPWNTYVYAVEKGKS
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001017919
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017919.1, NP_001017919.1
• RefSeq Size: 2690 bp
• RefSeq ORF: 1131 bp
• Locus ID: 91433
• UniProt ID: A6NED2
• Cytogenetics: 15q26.1
• MW: 39.9 kDa
• Gene Summary: Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with KDM8 (PubMed:24981860). Possibly together with KDM8, is involved in proper mitotic spindle organization and chromosome segregation (PubMed:24981860). Plays a role in regulating alpha-tubulin deacetylation and cytoskeletal microtubule stability, thereby promoting cell migration and TGF-beta-induced epithelial to mesenchymal transition (EMT), potentially through the inhibition of KDM8 (PubMed:28455245).[UniProtKB/Swiss-Prot Function]