CARD11 (NM_032415) Human Tagged ORF Clone

CAT#: RC222740

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CARD11 (Myc-DDK-tagged)-Human caspase recruitment domain family, member 11 (CARD11)



  "NM_032415" in other vectors (6)

Reconstitution Protocol

USD 1,506.00


Availability*
In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CARD11
Synonyms BENTA; BIMP3; CARMA1; IMD11; IMD11A; PPBL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222740 representing NM_032415.
Blue=ORF Red=Cloning site Green=Tag(s)

GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCAGTA
CCGAGGAGATCTGCGCCGCGATCGCCGGCGCGCCC
ATGCCAGGAGGAGGGCCAGAGATGGATGACTACATGGAGACGCTGAAGGATGAAGAGGACGCCTTGTGG
GAGAATGTGGAGTGTAACCGGCACATGCTCAGCCGCTATATCAACCCTGCCAAGCTCACGCCCTACCTG
CGTCAGTGTAAGGTCATTGATGAGCAGGATGAAGATGAAGTGCTTAATGCCCCTATGCTGCCATCCAAG
ATCAACCGAGCAGGCCGGCTGTTGGACATTCTACATACCAAGGGGCAAAGGGGCTATGTGGTCTTCTTG
GAGAGCCTAGAATTTTATTACCCAGAACTGTACAAACTGGTGACTGGGAAAGAGCCCACTCGGAGATTC
TCCACCATTGTGGTGGAGGAAGGCCACGAGGGCCTCACGCACTTCCTGATGAACGAGGTCATCAAGCTG
CAGCAGCAGATGAAGGCCAAGGACCTGCAACGCTGCGAGCTGCTGGCCAGGTTGCGGCAGCTGGAGGAT
GAGAAGAAGCAGATGACGCTGACGCGCGTGGAGCTGCTAACCTTCCAGGAGCGGTACTACAAGATGAAG
GAAGAGCGGGACAGCTACAATGACGAGCTGGTCAAGGTGAAGGACGACAACTACAACTTAGCCATGCGC
TACGCACAGCTCAGTGAGGAGAAGAACATGGCGGTCATGAGGAGCCGAGACCTCCAACTCGAGATCGAT
CAGCTAAAGCACCGGTTGAATAAGATGGAGGAGGAATGTAAGCTGGAGAGAAATCAGTCTCTAAAACTG
AAGAATGACATTGAAAATCGGCCCAAGAAGGAGCAGGTTCTGGAACTGGAGCGGGAGAATGAAATGCTG
AAGACCAAAAACCAGGAGCTGCAGTCCATCATCCAGGCCGGGAAGCGCAGCCTGCCAGACTCAGACAAG
GCCATCCTGGACATCTTGGAACACGACCGCAAGGAGGCCCTGGAGGACAGGCAGGAGCTGGTCAACAGG
ATCTACAACCTGCAGGAGGAGGCCCGCCAGGCAGAGGAGCTGCGAGACAAGTACCTGGAGGAGAAGGAG
GACCTGGAGCTCAAGTGCTCGACCCTGGGAAAGGACTGTGAAATGTACAAGCACCGCATGAACACGGTC
ATGCTGCAGCTGGAGGAGGTGGAGCGGGAGCGGGACCAGGCCTTCCACTCCCGAGATGAAGCTCAGACA
CAGTACTCGCAGTGCTTAATCGAAAAGGACAAGTACAGGAAGCAGATCCGCGAGCTGGAGGAGAAGAAC
GATGAGATGAGGATCGAGATGGTGCGGCGGGAGGCCTGCATCGTCAACCTGGAGAGCAAGCTGCGGCGC
CTCTCCAAGGACAGCAACAACCTGGACCAGAGTCTGCCCAGGAACCTGCCAGTAACCATCATCTCTCAG
GACTTTGGGGATGCCAGCCCCAGGACCAATGGTCAAGAAGCTGACGATTCTTCCACCTCGGAGGAGTCA
CCTGAAGACAGCAAGTACTTCCTGCCCTACCATCCGCCCCAGCGCAGGATGAACCTGAAGGGCATCCAG
CTGCAGAGAGCCAAATCCCCCATCAGCCTGAAGCGAACATCAGATTTTCAAGCCAAGGGGCACGAGGAA
GAAGGCACGGATGCCAGCCCTAGCTCCTGCGGATCTCTGCCCATCACCAACTCCTTCACCAAGATGCAG
CCCCCCCGGAGCCGCAGCAGCATCATGTCAATCACCGCCGAGCCCCCGGGAAACGACTCCATCGTCAGA
CGCTACAAGGAGGACGCGCCCCATCGCAGCACAGTCGAAGAAGACAATGACAGCGGCGGGTTTGACGCC
TTAGATCTGGATGATGACAGTCACGAACGCTACTCCTTCGGACCCTCCTCCATCCACTCCTCCTCCTCC
TCCCACCAATCCGAGGGCCTGGATGCCTACGACCTGGAGCAGGTCAACCTCATGTTCAGGAAGTTCTCT
CTGGAAAGACCCTTCCGGCCTTCGGTCACCTCTGTGGGGCACGTGCGGGGCCCAGGGCCCTCGGTGCAG
CACACGACGCTGAATGGCGACAGCCTCACCTCCCAGCTCACCCTGCTGGGGGGCAACGCGCGAGGGAGC
TTCGTGCACTCGGTCAAGCCTGGCTCTCTGGCCGAGAAAGCCGGCCTCCGTGAGGGCCACCAGCTGCTG
CTGCTAGAAGGCTGCATCCGAGGCGAGAGGCAGAGTGTCCCGTTGGACACATGCACCAAAGAGGAAGCC
CACTGGACCATCCAGAGGTGCAGCGGCCCCGTCACGCTGCACTACAAGGTCAACCACGAAGGGTACCGG
AAGCTGGTGAAGGACATGGAGGACGGCCTGATCACATCGGGGGACTCGTTCTACATCCGGCTGAACCTG
AACATCTCCAGCCAGCTGGACGCCTGCACCATGTCCCTGAAGTGTGACGATGTTGTGCACGTCCGTGAC
ACCATGTACCAGGACAGGCACGAGTGGCTGTGCGCGCGGGTCGACCCTTTCACAGACCATGACCTGGAT
ATGGGCACCATACCCAGCTACAGCCGAGCCCAGCAGCTCCTCCTGGTGAAACTGCAGCGCCTGATGCAC
CGAGGCAGCCGGGAGGAGGTAGACGGCACCCACCACACCCTGCGGGCACTCCGGAACACCCTGCAGCCA
GAAGAAGCGCTTTCAACAAGCGACCCCCGGGTCAGCCCCCGTCTCTCGCGAGCAAGCTTCCTTTTTGGC
CAGCTCCTTCAGTTCGTCAGCAGGTCCGAGAACAAGTATAAGCGGATGAACAGCAACGAGCGGGTCCGC
ATCATCTCGGGGAGTCCGCTAGGGAGCCTGGCCCGGTCCTCGCTGGACGCCACCAAGCTCTTGACTGAG
AAGCAGGAAGAGCTGGACCCTGAGAGCGAGCTGGGCAAGAACCTCAGCCTCATCCCCTACAGCCTGGTA
CGCGCCTTCTACTGCGAGCGCCGCCGGCCCGTGCTCTTCACACCCACCGTGCTGGCCAAGACGCTGGTG
CAGAGGCTGCTCAACTCGGGAGGTGCCATGGAGTTCACCATCTGCAAGTCAGATATCGTCACAAGAGAT
GAGTTCCTCAGAAGGCAGAAGACGGAGACCATCATCTACTCCCGAGAGAAGAACCCCAACGCGTTCGAA
TGCATCGCCCCTGCCAACATTGAAGCTGTGGCCGCCAAGAACAAGCACTGCCTGCTGGAGGCTGGGATC
GGCTGCACAAGAGACTTGATCAAGTCCAACATCTACCCCATCGTGCTCTTCATCCGGGTGTGTGAGAAG
AACATCAAGAGGTTCAGAAAGCTGCTGCCCCGACCTGAGACGGAGGAGGAGTTCCTGCGCGTGTGCCGG
CTGAAGGAGAAGGAGCTGGAGGCCCTGCCGTGCCTGTACGCCACGGTGGAACCTGACATGTGGGGCAGC
GTAGAGGAGCTGCTCCGCGTTGTCAAGGACAAGATCGGCGAGGAGCAGCGCAAGACCATCTGGGTGGAC
GAGGACCAGCTG

AGCGGTCCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
ATCCTGGATTACAAGGATGACGACGATAAG
GTTTAA
>Peptide sequence encoded by RC222740
Blue=ORF Red=Cloning site Green=Tag(s)

MPGGGPEMDDYMETLKDEEDALWENVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNAPMLPSK
INRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEVIKL
QQQMKAKDLQRCELLARLRQLEDEKKQMTLTRVELLTFQERYYKMKEERDSYNDELVKVKDDNYNLAMR
YAQLSEEKNMAVMRSRDLQLEIDQLKHRLNKMEEECKLERNQSLKLKNDIENRPKKEQVLELERENEML
KTKNQELQSIIQAGKRSLPDSDKAILDILEHDRKEALEDRQELVNRIYNLQEEARQAEELRDKYLEEKE
DLELKCSTLGKDCEMYKHRMNTVMLQLEEVERERDQAFHSRDEAQTQYSQCLIEKDKYRKQIRELEEKN
DEMRIEMVRREACIVNLESKLRRLSKDSNNLDQSLPRNLPVTIISQDFGDASPRTNGQEADDSSTSEES
PEDSKYFLPYHPPQRRMNLKGIQLQRAKSPISLKRTSDFQAKGHEEEGTDASPSSCGSLPITNSFTKMQ
PPRSRSSIMSITAEPPGNDSIVRRYKEDAPHRSTVEEDNDSGGFDALDLDDDSHERYSFGPSSIHSSSS
SHQSEGLDAYDLEQVNLMFRKFSLERPFRPSVTSVGHVRGPGPSVQHTTLNGDSLTSQLTLLGGNARGS
FVHSVKPGSLAEKAGLREGHQLLLLEGCIRGERQSVPLDTCTKEEAHWTIQRCSGPVTLHYKVNHEGYR
KLVKDMEDGLITSGDSFYIRLNLNISSQLDACTMSLKCDDVVHVRDTMYQDRHEWLCARVDPFTDHDLD
MGTIPSYSRAQQLLLVKLQRLMHRGSREEVDGTHHTLRALRNTLQPEEALSTSDPRVSPRLSRASFLFG
QLLQFVSRSENKYKRMNSNERVRIISGSPLGSLARSSLDATKLLTEKQEELDPESELGKNLSLIPYSLV
RAFYCERRRPVLFTPTVLAKTLVQRLLNSGGAMEFTICKSDIVTRDEFLRRQKTETIIYSREKNPNAFE
CIAPANIEAVAAKNKHCLLEAGIGCTRDLIKSNIYPIVLFIRVCEKNIKRFRKLLPRPETEEEFLRVCR
LKEKELEALPCLYATVEPDMWGSVEELLRVVKDKIGEEQRKTIWVDEDQL

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV

Recombinant protein using RC222740 also available, TP322740
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites AscI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_032415
ORF Size 3462 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq Size 4372 bp
RefSeq ORF 3465 bp
Locus ID 84433
UniProt ID Q9BXL7
Cytogenetics 7p22.2
Protein Families Druggable Genome
Protein Pathways B cell receptor signaling pathway, T cell receptor signaling pathway
MW 133.3 kDa
Gene Summary The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq, Jul 2008]

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