TAP2 (NM_018833) Human Tagged ORF Clone

CAT#: RC222668

TAP2 (Myc-DDK-tagged)-Human transporter 2, ATP-binding cassette, sub-family B (MDR/TAP) (TAP2), transcript variant 2

Product Images

Circular map for RC222668

• 

  Western blot validation of overexpression lysate (Cat# LY412873) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222668 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAP2
• Synonyms: ABC18; ABCB3; APT2; D6S217E; PSF-2; PSF2; RING11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222668 representing NM_018833
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTCCCTGACCTGAGACCCTGGACCTCCCTGCTGCTGGTGGACGCGGCTTTACTGTGGCTGCTTC
  AGGGCCCTCTGGGGACTTTGCTTCCTCAAGGGCTGCCAGGACTATGGCTGGAGGGGACCCTGCGGCTGGG
  AGGGCTGTGGGGGCTGCTAAAGCTAAGAGGGCTGCTGGGATTTGTGGGGACACTGCTGCTCCCGCTCTGT
  CTGGCCACCCCCCTGACTGTCTCCCTGAGAGCCCTGGTCGCGGGGGCCTCACGTGCTCCCCCAGCCAGAG
  TCGCTTCAGCCCCTTGGAGCTGGCTGCTGGTGGGGTACGGGGCTGCGGGGCTCAGCTGGTCACTGTGGGC
  TGTTCTGAGCCCTCCTGGAGCCCAGGAGAAGGAGCAGGACCAGGTGAACAACAAAGTCTTGATGTGGAGG
  CTGCTGAAGCTCTCCAGGCCGGACCTGCCTCTCCTCGTTGCCGCCTTCTTCTTCCTTGTCCTTGCTGTTT
  TGGGTGAGACATTAATCCCTCACTATTCTGGTCGTGTGATTGACATCCTGGGAGGTGATTTTGACCCCCA
  TGCCTTTGCCAGTGCCATCTTCTTCATGTGCCTCTTCTCCTTTGGCAGCTCACTGTCTGCAGGCTGCCGA
  GGAGGCTGCTTCACCTACACCATGTCTCGAATCAACTTGCGGATCCGGGAGCAGCTTTTCTCCTCCCTGC
  TGCGCCAGGACCTCGGTTTCTTCCAGGAGACTAAGACAGGGGAGCTGAACTCACGGCTGAGCTCGGATAC
  CACCCTGATGAGTAACTGGCTTCCTTTAAATGCCAATGTGCTCTTGCGAAGCCTGGTGAAAGTGGTGGGG
  CTGTATGGCTTCATGCTCAGCATATCGCCTCGACTCACCCTCCTTTCTCTGCTGCACATGCCCTTCACAA
  TAGCAGCGGAGAAGGTGTACAACACCCGCCATCAGGAAGTGCTTCGGGAGATCCAGGATGCAGTGGCCAG
  GGCGGGGCAGGTGGTGCGGGAAGCCGTTGGAGGGCTGCAGACCGTTCGCAGTTTTGGGGCCGAGGAGCAT
  GAAGTCTGTCGCTATAAAGAGGCCCTTGAACAATGTCGGCAGCTGTATTGGCGGAGAGACCTGGAACGCG
  CCTTGTACCTGCTCGTAAGGAGGGTGCTGCACTTGGGGGTGCAGATGCTGATGCTGAGCTGTGGGCTGCA
  GCAGATGCAGGATGGGGAGCTCACCCAGGGCAGCCTGCTTTCCTTTATGATCTACCAGGAGAGCGTGGGG
  AGCTATGTGCAGACCCTGGTATACATATATGGGGATATGCTCAGCAACGTGGGAGCTGCAGAGAAGGTTT
  TCTCCTACATGGACCGACAGCCAAATCTGCCTTCACCTGGCACGCTTGCCCCCACCACTCTGCAGGGGGT
  TGTGAAATTCCAAGACGTCTCCTTTGCATATCCCAATCGCCCTGACAGGCCTGTGCTCAAGGGGCTGACG
  TTTACCCTACGTCCTGGTGAGGTGACGGCGCTGGTGGGACCCAATGGGTCTGGGAAGAGCACAGTGGCTG
  CCCTGCTGCAGAATCTGTACCAGCCCACAGGGGGACAGGTGCTGCTGGATGAAAAGCCCATCTCACAGTA
  TGAACACTGCTACCTGCACAGCCAGGTGGTTTCAGTTGGGCAGGAGCCTGTGCTGTTCTCCGGTTCTGTG
  AGGAACAACATTGCTTATGGGCTGCAGAGCTGCGAAGATGATAAGGTGATGGCGGCTGCCCAGGCTGCCC
  ACGCAGATGACTTCATCCAGGAAATGGAGCATGGAATATACACAGATGTAGGGGAGAAGGGAAGCCAGCT
  GGCTGCGGGACAGAAACAACGTCTGGCCATTGCCCGGGCCCTTGTACGAGACCCGCGGGTCCTCATCCTG
  GATGAGGCTACTAGTGCCCTAGATGTGCAGTGCGAGCAGGCCAAAACCCTTTGGAAGTTCATGATATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222668 representing NM_018833
  Red=Cloning site Green=Tags(s)
  
  MRLPDLRPWTSLLLVDAALLWLLQGPLGTLLPQGLPGLWLEGTLRLGGLWGLLKLRGLLGFVGTLLLPLC
  LATPLTVSLRALVAGASRAPPARVASAPWSWLLVGYGAAGLSWSLWAVLSPPGAQEKEQDQVNNKVLMWR
  LLKLSRPDLPLLVAAFFFLVLAVLGETLIPHYSGRVIDILGGDFDPHAFASAIFFMCLFSFGSSLSAGCR
  GGCFTYTMSRINLRIREQLFSSLLRQDLGFFQETKTGELNSRLSSDTTLMSNWLPLNANVLLRSLVKVVG
  LYGFMLSISPRLTLLSLLHMPFTIAAEKVYNTRHQEVLREIQDAVARAGQVVREAVGGLQTVRSFGAEEH
  EVCRYKEALEQCRQLYWRRDLERALYLLVRRVLHLGVQMLMLSCGLQQMQDGELTQGSLLSFMIYQESVG
  SYVQTLVYIYGDMLSNVGAAEKVFSYMDRQPNLPSPGTLAPTTLQGVVKFQDVSFAYPNRPDRPVLKGLT
  FTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQVLLDEKPISQYEHCYLHSQVVSVGQEPVLFSGSV
  RNNIAYGLQSCEDDKVMAAAQAAHADDFIQEMEHGIYTDVGEKGSQLAAGQKQRLAIARALVRDPRVLIL
  DEATSALDVQCEQAKTLWKFMIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018833
• ORF Size: 1959 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018833.2, NP_061313.2
• RefSeq Size: 2540 bp
• RefSeq ORF: 1962 bp
• Locus ID: 6891
• UniProt ID: Q03519
• Cytogenetics: 6p21.32
• Domains: ABC_membrane, ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters, Antigen processing and presentation, Primary immunodeficiency
• MW: 71.8 kDa
• Gene Summary: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. This gene is located 7 kb telomeric to gene family member ABCB2. The protein encoded by this gene is involved in antigen presentation. This protein forms a heterodimer with ABCB2 in order to transport peptides from the cytoplasm to the endoplasmic reticulum. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Alternative splicing of this gene produces products which differ in peptide selectivity and level of restoration of surface expression of MHC class I molecules. [provided by RefSeq, Feb 2014]