EFS (NM_005864) Human Tagged ORF Clone

CAT#: RC222649

EFS (Myc-DDK-tagged)-Human embryonal Fyn-associated substrate (EFS), transcript variant 1

Product Images

Circular map for RC222649

• 

  Western blot validation of overexpression lysate (Cat# LY417016) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222649 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EFS
• Synonyms: CAS3; CASS3; EFS1; EFS2; HEFS; SIN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222649 representing NM_005864
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATTGCCACGTCGACCCAGCTGGCCCGGGCACTGTATGACAACACCGCTGAGTCCCCCCAGGAGC
  TGTCCTTCCGCCGAGGGGATGTCCTACGGGTCCTGCAGAGAGAGGGCGCTGGTGGACTGGACGGCTGGTG
  CCTCTGCTCCCTACACGGCCAGCAGGGCATTGTGCCCGCCAACAGGGTGAAGCTCTTGCCTGCTGGCCCA
  GCACCCAAGCCCAGCCTCTCTCCTGCGTCCCCAGCCCAGCCTGGCTCACCATATCCAGCCCCAGATCACA
  GCAATGAGGACCAGGAGGTGTATGTGGTGCCGCCCCCAGCTCGGCCCTGTCCAACCTCAGGACCTCCAGC
  TGGACCTTGCCCACCCTCTCCTGACCTCATCTACAAAATCCCCAGAGCTAGTGGGACCCAGCTGGCTGCT
  CCCAGAGATGCCTTGGAGGTCTACGATGTGCCCCCCACCGCCCTCCGGGTGCCCTCCAGTGGCCCCTATG
  ACTGCCCTGCCTCCTTTTCCCACCCTCTGACCCGGGTTGCCCCGCAGCCCCCTGGAGAGGATGATGCTCC
  CTATGATGTGCCTCTGACCCCAAAGCCACCTGCAGAGCTGGAACCAGATCTGGAGTGGGAAGGAGGCCGG
  GAGCCGGGGCCCCCCATCTATGCTGCCCCCTCCAACCTGAAACGAGCGTCAGCCTTACTCAATTTGTATG
  AAGCACCCGAGGAACTGCTGGCAGACGGGGAGGGCGGGGGCACTGATGAGGGGATCTACGATGTGCCTCT
  GCTGGGGCCAGAGGCTCCCCCTTCTCCAGAGCCCCCTGGAGCCTTGGCCTCCCATGACCAGGACACCCTG
  GCCCAGCTTCTGGCCAGAAGCCCCCCACCCCCACACAGGCCCCGGCTCCCCTCAGCTGAGAGCCTGTCCC
  GCCGCCCTCTGCCTGCCCTGCCTGTCCCTGAGGCCCCCAGCCCCTCCCCAGTGCCCTCTCCTGCCCCAGG
  CCGGAAGGGCAGCATCCAGGACCGGCCTCTGCCCCCACCCCCACCCCGCCTGCCTGGTTATGGAGGCCCC
  AAGGTCGAGGGGGATCCAGAGGGCAGGGAGATGGAGGATGACCCAGCAGGACACCACAATGAGTACGAGG
  GCATTCCGATGGCCGAGGAGTATGACTATGTCCACCTGAAGGGCATGGACAAAGCTCAGGGATCTAGGCC
  CCCGGATCAGGCCTGCACAGGGGATCCTGAACTGCCCGAGAGGGGGATGCCGGCGCCGCAGGAGGCCCTG
  TCCCCAGGGGAGCCACTGGTTGTGTCCACCGGAGATCTGCAGCTCCTGTACTTCTATGCTGGGCAATGCC
  AGAGCCACTACTCAGCCCTGCAGGCAGCCGTGGCAGCCCTGATGTCCAGTACCCAGGCTAATCAGCCCCC
  GCGCCTTTTCGTGCCCCACAGCAAGAGGGTGGTGGTGGCTGCTCATCGCCTGGTGTTTGTTGGGGACACC
  CTGGGCCGGCTGGCAGCCTCTGCCCCTCTGAGAGCACAGGTCAGGGCTGCAGGTACAGCACTGGGCCAGG
  CATTGCGGGCCACTGTGCTGGCTGTCAAGGGAGCTGCCCTGGGCTACCCATCCAGCCCTGCCATCCAAGA
  GATGGTGCAGTGTGTAACAGAACTGGCAGGGCAGGCCCTGCAATTCACTACCCTGCTCACTAGCCTGGCT
  CCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222649 representing NM_005864
  Red=Cloning site Green=Tags(s)
  
  MAIATSTQLARALYDNTAESPQELSFRRGDVLRVLQREGAGGLDGWCLCSLHGQQGIVPANRVKLLPAGP
  APKPSLSPASPAQPGSPYPAPDHSNEDQEVYVVPPPARPCPTSGPPAGPCPPSPDLIYKIPRASGTQLAA
  PRDALEVYDVPPTALRVPSSGPYDCPASFSHPLTRVAPQPPGEDDAPYDVPLTPKPPAELEPDLEWEGGR
  EPGPPIYAAPSNLKRASALLNLYEAPEELLADGEGGGTDEGIYDVPLLGPEAPPSPEPPGALASHDQDTL
  AQLLARSPPPPHRPRLPSAESLSRRPLPALPVPEAPSPSPVPSPAPGRKGSIQDRPLPPPPPRLPGYGGP
  KVEGDPEGREMEDDPAGHHNEYEGIPMAEEYDYVHLKGMDKAQGSRPPDQACTGDPELPERGMPAPQEAL
  SPGEPLVVSTGDLQLLYFYAGQCQSHYSALQAAVAALMSSTQANQPPRLFVPHSKRVVVAAHRLVFVGDT
  LGRLAASAPLRAQVRAAGTALGQALRATVLAVKGAALGYPSSPAIQEMVQCVTELAGQALQFTTLLTSLA
  P
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005864
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005864.4
• RefSeq Size: 3132 bp
• RefSeq ORF: 1686 bp
• Locus ID: 10278
• UniProt ID: O43281
• Cytogenetics: 14q11.2
• MW: 58.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the CAS (CRK-associated substrate) family of adaptor proteins which typically serve as scaffolds for the assembly of larger signaling complexes. These complexes form at the cell surface where integrin binding leads to the subsequent phosphorylation of a CAS protein. Additional binding of SRC family kinases leads to CAS hyperphosphorylation and the creation of binding sites for CRK and other proteins that cause actin cytoskeleton reorganization. This gene plays a role in integrin-mediated cell attachment, spreading, and migration and also plays a role in both normal and malignant cellular transformation. This broadly expressed gene has been shown to play a role in neurite outgrowth and its expression in the thymus and lymphocytes is important for T cell maturation and the development of immunological self-tolerance. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2020]