USP15 (NM_006313) Human Tagged ORF Clone

CAT#: RC222642

Reviews ()
Write a review

USP15 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 15 (USP15)



  "NM_006313" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 851.00


Availability*
In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol USP15
Synonyms UNPH-2; UNPH4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC222642 representing NM_006313
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAAGGCGGAGCGGCGGATCTGGACACCCAGCGGTCTGACATCGCGACGCTGCTCAAAACCTCGC
TCCGGAAAGGGGACACCTGGTACCTAGTCGATAGTCGCTGGTTCAAACAGTGGAAAAAATATGTTGGCTT
TGACAGTTGGGACAAATACCAGATGGGAGATCAAAATGTGTATCCTGGACCCATTGATAACTCTGGACTT
CTCAAAGATGGTGATGCCCAGTCACTTAAGGAACACCTTATTGATGAATTGGATTACATACTGTTGCCAA
CTGAAGGTTGGAATAAACTTGTCAGCTGGTACACATTGATGGAAGGTCAAGAGCCAATAGCACGAAAGGT
GGTTGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTATATCTCACAGAATTGAAGCTATGTGAA
AATGGAAACATGAATAATGTTGTAACTCGAAGATTTAGCAAAGCTGACACAATAGATACAATTGAAAAGG
AAATAAGAAAAATCTTCAGTATTCCAGATGAAAAGGAGACCAGATTGTGGAACAAATACATGAGTAACAC
ATTTGAACCACTGAATAAACCAGACAGCACCATTCAGGATGCTGGTTTATACCAAGGACAGGTATTAGTG
ATAGAACAGAAAAATGAAGATGGAACATGGCCAAGGGGTCCTTCTACTCCTAATGTGAAAAACTCAAATT
ACTGTCTTCCATCATATACCGCTTATAAGAACTATGATTATTCGGAACCTGGAAGAAACAATGAACAGCC
AGGCCTCTGTGGCCTAAGTAACTTGGGAAATACGTGTTTCATGAACTCAGCTATTCAGTGTTTGAGCAAC
ACACCTCCACTTACTGAGTATTTCCTCAATGATAAGTATCAAGAAGAACTGAATTTTGACAATCCCTTAG
GAATGAGAGGTGAAATAGCTAAATCTTATGCCGAACTGATCAAGCAAATGTGGTCTGGAAAGTTTAGCTA
CGTCACCCCAAGAGCCTTTAAGACACAGGTAGGACGTTTTGCACCTCAGTTCTCTGGATATCAGCAGCAA
GACTGTCAAGAACTGTTAGCTTTCCTATTAGATGGATTACATGAGGATTTGAATAGAATTAGGAAAAAAC
CATATATACAATTAAAAGATGCAGATGGAAGGCCAGATAAGGTGGTTGCCGAAGAAGCCTGGGAAAACCA
TTTAAAACGAAATGATTCTATCATAGTAGATATATTTCATGGCCTTTTCAAATCAACTTTAGTTTGTCCT
GAGTGTGCTAAGATTTCAGTAACATTTGATCCTTTTTGTTACTTGACACTTCCATTGCCCATGAAAAAAG
AACGCACCTTGGAAGTTTACTTAGTTAGAATGGATCCACTTACCAAACCTATGCAGTACAAAGTGGTTGT
CCCCAAAATTGGAAACATATTAGATCTTTGTACAGCATTGTCTGCTTTGTCAGGAATACCTGCAGATAAG
ATGATAGTTACTGATATATACAATCATAGATTTCACAGAATATTCGCTATGGATGAAAACCTTAGTAGTA
TTATGGAACGGGATGATATTTATGTGTTTGAAATTAACATCAATAGGACAGAAGATACAGAGCACGTGAT
TATTCCTGTTTGCCTAAGAGAAAAATTCAGACACTCGAGTTATACCCACCATACTGGTTCTTCACTTTTT
GGTCAGCCCTTTCTTATGGCTGTACCACGAAACAATACTGAAGACAAACTTTATAATCTCCTGCTCTTGA
GAATGTGCCGATATGTCAAAATATCTACTGAAACTGAAGAAACTGAAGGATCCCTACACTGCTGTAAGGA
CCAAAATATTAATGGGAATGGCCCAAATGGCATACATGAAGAAGGCTCACCAAGTGAAATGGAAACAGAT
GAGCCAGATGATGAATCCAGCCAGGATCAAGAACTTCCCTCAGAGAATGAAAACAGTCAGTCTGAAGATT
CAGTTGGAGGAGATAATGATTCTGAAAATGGATTATGTACTGAGGATACTTGCAAAGGTCAACTCACGGG
ACACAAAAAACGATTGTTTACATTCCAGTTCAACAACTTAGGCAATACTGATATCAACTACATCAAAGAT
GATACCAGGCATATAAGATTTGATGATAGGCAGCTTAGGCTAGATGAAAGATTTTTTCTTGCTTTGGATT
GGGATCCTGATTTGAAAAAAAGATATTTTGATGAAAATGCTGCCGAGGACTTTGAAAAACATGAAAGTGT
GGAGTATAAACCTCCTAAAAAACCCTTTGTGAAATTAAAAGATTGCATTGAACTTTTTACAACAAAAGAA
AAGCTAGGTGCTGAAGATCCCTGGTATTGTCCGAATTGTAAAGAACATCAGCAAGCCACAAAGAAATTGG
ATTTATGGTCCCTGCCTCCAGTACTTGTAGTACATCTCAAGCGATTTTCTTACAGTCGATACATGAGAGA
CAAGTTGGATACCTTAGTTGATTTTCCTATCAATGACTTGGATATGTCGGAATTCTTAATTAATCCAAAT
GCAGGTCCTTGCCGCTATAATCTGATTGCTGTTTCCAACCACTATGGAGGGATGGGAGGAGGACACTATA
CTGCTTTTGCAAAAAATAAAGATGATGGAAAATGGTACTATTTTGATGACAGTAGTGTCTCCACTGCATC
TGAAGACCAAATTGTGTCCAAAGCAGCATATGTACTCTTCTACCAGAGACAAGACACTTTCAGTGGAACT
GGCTTTTTTCCTCTTGACCGAGAAACTAAAGGTGCTTCAGCTGCCACTGGCATCCCATTAGAAAGTGATG
AAGATAGCAATGATAATGACAATGATATAGAAAATGAAAACTGTATGCACACTAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC222642 representing NM_006313
Red=Cloning site Green=Tags(s)

MAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGL
LKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCE
NGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKETRLWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLV
IEQKNEDGTWPRGPSTPNVKNSNYCLPSYTAYKNYDYSEPGRNNEQPGLCGLSNLGNTCFMNSAIQCLSN
TPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKFSYVTPRAFKTQVGRFAPQFSGYQQQ
DCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCP
ECAKISVTFDPFCYLTLPLPMKKERTLEVYLVRMDPLTKPMQYKVVVPKIGNILDLCTALSALSGIPADK
MIVTDIYNHRFHRIFAMDENLSSIMERDDIYVFEININRTEDTEHVIIPVCLREKFRHSSYTHHTGSSLF
GQPFLMAVPRNNTEDKLYNLLLLRMCRYVKISTETEETEGSLHCCKDQNINGNGPNGIHEEGSPSEMETD
EPDDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEDTCKGQLTGHKKRLFTFQFNNLGNTDINYIKD
DTRHIRFDDRQLRLDERFFLALDWDPDLKKRYFDENAAEDFEKHESVEYKPPKKPFVKLKDCIELFTTKE
KLGAEDPWYCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDTLVDFPINDLDMSEFLINPN
AGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDSSVSTASEDQIVSKAAYVLFYQRQDTFSGT
GFFPLDRETKGASAATGIPLESDEDSNDNDNDIENENCMHTN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006313
ORF Size 2856 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006313.3
RefSeq Size 4611 bp
RefSeq ORF 2859 bp
Locus ID 9958
UniProt ID Q9Y4E8
Cytogenetics 12q14.1
Domains UCH, DUSP
Protein Families Druggable Genome, Protease
MW 109.1 kDa
Gene Summary This gene encodes a member of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. USP enzymes play critical roles in ubiquitin-dependent processes through polyubiquitin chain disassembly and hydrolysis of ubiquitin-substrate bonds. The encoded protein associates with the COP9 signalosome, and also plays a role in transforming growth factor beta signalling through deubiquitination of receptor-activated SMAD transcription factors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 2. [provided by RefSeq, Nov 2011]

Other Versions

Other products for "USP15"
Frequently bought together (5)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 30 ul

USD 165.00


pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

USD 627.00


Rabbit Polyclonal antibody to USP15 (ubiquitin specific peptidase 15)
    • 100 ul

USD 473.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

USD 149.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

USD 55.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.