Keratin 39 (KRT39) (NM_213656) Human Tagged ORF Clone

CAT#: RC222547

KRT39 (Myc-DDK-tagged)-Human keratin 39 (KRT39)

Product Images

Circular map for RC222547

• 

  Western blot validation of overexpression lysate (Cat# LY403734) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222547 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Keratin 39
• Synonyms: CK-39; K39; KA35
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222547 representing NM_213656
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACCAAGGGCTGTACAACAACCAATTCTCCTTCAACCCCATGTCAAAACTGCTCTAGGATTACAA
  ATGTTAGTACCATCTCTTCTAACAACGGCTGCCATCCTGGTGGCCTTACAGTCAACAACTGTCAACCAGC
  TGGCCACGTTCTCAGAATTCCCTGGGACCAGGGCTGCCAACCCACTCCTCGCTTTTGTCGCAAGCCCATC
  TACCTAATGAACAACTTCAATGCCCGTTTTTCTCTGGATGACTGCAGCTGGTATGGTGAAGGCATCAACA
  GTAATGAGAAGGAGACCATGCAAATCTTGAACGAGCGCCTTGCTAACTACCTGCAAAAGGTGCGAATGCT
  AGAACGAGAGAATGCTGAACTGGAATCTAAAATCCAGGAAGAAAGTAACAAAGAGCTCCCTGTTCTATGT
  CCTGATTACCTGTCTTACTACACTACCATTGAGGAGCTCCAGCAGAAGATCTTGTGTACCAAGGCCGAGA
  ATTCCAGACTGGTCTCGCAAATTGACAACACCAAACTGACTGCAGATGACTTGAGAGCCAAATACGAAGC
  TGAGGTGTCTCTACGCCAGCTGGTAGAGTCAGATGCCAATGGCCTCAAGCAGATCCTGAATGTGCTGACC
  CTGGGCAAGGCCGACCTAGAGGCACAAGTCCAGTCTCTGAAAGAGGAGCTCCTTTGCCTCAAGAACAACC
  ACAAAGAGGAAATCAATTCTTTACAGTGTCAGCTTGGGGAGAGACTTGACATTGAAGTGACTGCTGCCCC
  TTCTGCTGACCTAAACCAGGTTCTACAAGAAATGAGATGTCAATATGAGCCCATCATGGAGACAAACCGC
  AAAGATGTGGAACAGTGGTTCAACACGCAGATAGAGGAGCTGAATCAACAAGTGGTGACCAGCTCTCAAC
  AGCAGCAATGCTGCCAAAAGGAGATCATAGAACTGAGACGCAGTGTGAACACTCTGGAGGTTGAACTGCA
  GGCCCAGCATCGAATGAGAGATTCCCAAGAGTGCATCCTAACGGAGACAGAGGCTCGCTACACGGCCTTG
  CTGACCCAGATCCAGAGTCTGATTGATAACCTGGAAGCTCAGCTGGCAGAGATCCGGTGTGCCCTGGAAA
  GACAGAACCAAGAATACGAGATCCTGCTGGACGTCAAGTCCCGGCTGGAATGTGAGATTACCACATACCG
  CAGCCTTCTGGAGAGCTCGGATGGCAAGCGTCCCTGTTACCCACGTGCCACCAAATGTGAGCCTTCCCCT
  TGGACATCTTGTAAGTCCGGAGCCATAGAAAGCACGGCCCCAGCTTGCACATCCTCATCCCCCTGCAGCT
  TAAAGGAGCACTGCAGTGCCTGCGGACCCCTGTCCCGGATACTGGTTAAAATTTGCACCATCACCAAGGA
  GATTAAGGATGGGAAGGTCATTTCTTCTTACGAGCATGTGCAGCCTTGTTTCATCATCAGACCTGCCAAA
  GTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222547 representing NM_213656
  Red=Cloning site Green=Tags(s)
  
  MDTKGCTTTNSPSTPCQNCSRITNVSTISSNNGCHPGGLTVNNCQPAGHVLRIPWDQGCQPTPRFCRKPI
  YLMNNFNARFSLDDCSWYGEGINSNEKETMQILNERLANYLQKVRMLERENAELESKIQEESNKELPVLC
  PDYLSYYTTIEELQQKILCTKAENSRLVSQIDNTKLTADDLRAKYEAEVSLRQLVESDANGLKQILNVLT
  LGKADLEAQVQSLKEELLCLKNNHKEEINSLQCQLGERLDIEVTAAPSADLNQVLQEMRCQYEPIMETNR
  KDVEQWFNTQIEELNQQVVTSSQQQQCCQKEIIELRRSVNTLEVELQAQHRMRDSQECILTETEARYTAL
  LTQIQSLIDNLEAQLAEIRCALERQNQEYEILLDVKSRLECEITTYRSLLESSDGKRPCYPRATKCEPSP
  WTSCKSGAIESTAPACTSSSPCSLKEHCSACGPLSRILVKICTITKEIKDGKVISSYEHVQPCFIIRPAK
  V
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_213656
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_213656.4
• RefSeq Size: 1696 bp
• RefSeq ORF: 1476 bp
• Locus ID: 390792
• UniProt ID: Q6A163
• Cytogenetics: 17q21.2
• MW: 55.5 kDa
• Gene Summary: This gene encodes a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2009]