KRT26 (NM_181539) Human Tagged ORF Clone

CAT#: RC222282

KRT26 (Myc-DDK-tagged)-Human keratin 26 (KRT26)

Product Images

Circular map for RC222282

• 

  Western blot validation of overexpression lysate (Cat# LY405700) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222282 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KRT26
• Synonyms: CK26; K25B; K25IRS2; K26; KRT25B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222282 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTTCGACTTTCTGGTGGATCCAGGAGGATCTGCTCGCGAACTGGGTCTGGTAGGCTGTCCGGTG
  GAGGAACAGGCTTCGTGGCTGGGAATGTGTGTGTTGGATCGGGAGCAAGAAGCAGCTTTTCTTGTACTCT
  TGAGGGCATCTCTTCTGGAGGAAGCTTCTGCAATAGCGGTGGAGGGTTGGGAAGTGGTGCCTGTGCTGGT
  TTTCTTGGCAATGAGCACAGCCTCCTCTCTGGGAATGAAAAGGTGACCATGCAGAATCTCAACGACCGCC
  TGGCATCCTACCTGGACCATGTGCATGCTCTAGAGGAGGCCAACGCAGACCTGGAGCAGAAGATCAAGGG
  CTGGTACGAGAAATGTGAGCCTGGCTCTTCCCGGGAACACGATCATGACTATAGCAGATACTTCTCAGTC
  ATAGAAGATCTTAAAAGGCAGATTATTTCTGCGACCATCTGCAATGCCAGCATTGTTCTACAAAATGACA
  ATGCCAGACTGACCGCTGATGACTTCAGGCTGAAGTATGAAAATGAGCTGGCTCTGCACCACAGTGTTGA
  GGCCGACACCAGTGGTCTTCGCAGAGTGTTGGATGAACTGACCCTTTGTACAACCGACCTGGAGATACAG
  TGTGAGACCCTCAGTGAGGAATTGACCTACCTCAAAAAAAGTCATGAGGAGGAAATGGAAGTCTTGCAAT
  ATACAGCTGGGGGGAACGTGAACGTGGAGATGAATGCAACCCCAGGAGTGGACCTAACTGTCCTGTTGAA
  CAACATGAGGGCTGAGTATGAGGACTTGGCTGAGCAGAACCGCAAAGATGCTGAAGCCTGGTTCAACGAG
  AGGAGTGCAACGCTGCAACAACAGATTTCCGATCATGAGGGAGCAGCCACAGCAGCCAGAAATGAGCTGA
  CCGAATTAAAACGCAATCTGCAAACCCTGGAAATAGAACTTCAGTCCCTCATGGCTGTGAAACATTCCTA
  TGAATGCTCCTTGGCTGAGACTGAAGGAAATTACTGCAATCAACTCCAGCAAATTCAGGATCAGATAGGG
  GTGATGGAGGAACAACTGCAACAGATTCGAACAGAAACAGAAGGCCAGAAGCTGGAGTATGAACAGCTTC
  TTGATGTAAAAATATTTTTAGAAAAAGAAATTGACATTTATTGCAACTTACTAGATGGAGAAGAAAGAAA
  AAGCAAATCCACATGTTACAAATCAAAAGGATACAGGCCTGTGAATTCTGGAAATCAAGCCAAAGACTCA
  ACAGAAGAAACTATTGTTAAAACAGTGGTTGAGGAACTGGATCAAATTGGCAATCTCCTTTCACTGAGAG
  TCCACTCAGTTGAAGAAAAGTCCTCTAAAATAAGCAACATTACAGTAGAGCAACGAGTACCTTCTAAAGC
  ACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222282 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSFRLSGGSRRICSRTGSGRLSGGGTGFVAGNVCVGSGARSSFSCTLEGISSGGSFCNSGGGLGSGACAG
  FLGNEHSLLSGNEKVTMQNLNDRLASYLDHVHALEEANADLEQKIKGWYEKCEPGSSREHDHDYSRYFSV
  IEDLKRQIISATICNASIVLQNDNARLTADDFRLKYENELALHHSVEADTSGLRRVLDELTLCTTDLEIQ
  CETLSEELTYLKKSHEEEMEVLQYTAGGNVNVEMNATPGVDLTVLLNNMRAEYEDLAEQNRKDAEAWFNE
  RSATLQQQISDHEGAATAARNELTELKRNLQTLEIELQSLMAVKHSYECSLAETEGNYCNQLQQIQDQIG
  VMEEQLQQIRTETEGQKLEYEQLLDVKIFLEKEIDIYCNLLDGEERKSKSTCYKSKGYRPVNSGNQAKDS
  TEETIVKTVVEELDQIGNLLSLRVHSVEEKSSKISNITVEQRVPSKAP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181539
• ORF Size: 1404 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181539.4, NP_853517.2
• RefSeq Size: 1748 bp
• RefSeq ORF: 1407 bp
• Locus ID: 353288
• UniProt ID: Q7Z3Y9
• Cytogenetics: 17q21.2
• MW: 51.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the keratin superfamily. This keratin protein is a type I keratin that is specific for the inner root sheath of the hair follicle. This gene exists in a cluster with other keratin genes on chromosome 17q21. [provided by RefSeq, Jul 2009]