PI 3 Kinase Class 2A (PIK3C2A) (NM_002645) Human Tagged ORF Clone

CAT#: RC222192

PIK3C2A (Myc-DDK-tagged)-Human phosphoinositide-3-kinase, class 2, alpha polypeptide (PIK3C2A)

Product Images

Circular map for RC222192

• 

  Western blot validation of overexpression lysate (Cat# LY400940) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222192 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PI 3 Kinase Class 2A
• Synonyms: CPK; OCSKD; PI3-K-C2(ALPHA); PI3-K-C2A; PI3K-C2-alpha; PI3K-C2alpha
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222192 representing NM_002645
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCAGATATCTAGCAACAGCGGATTTAAAGAATGTCCATCTTCACATCCGGAACCAACAAGAGCAA
  AAGATGTGGACAAAGAAGAAGCATTACAGATGGAAGCAGAGGCTTTAGCAAAACTGCAAAAGGATAGACA
  AGTGACTGACAATCAGAGAGGCTTTGAGTTGTCAAGCAGCACCAGAAAAAAAGCACAGGTTTATAACAAG
  CAGGATTATGATCTCATGGTGTTTCCTGAATCAGATTCCCAAAAAAGAGCATTAGATATTGATGTAGAAA
  AGCTCACCCAAGCTGAACTTGAGAAACTATTGCTGGATGACAGTTTCGAGACTAAAAAAACACCTGTATT
  ACCAGTTACTCCTATTCTGAGCCCTTCCTTTTCAGCACAGCTCTATTTTAGACCTACTATTCAGAGAGGA
  CAGTGGCCACCTGGATTACCTGGGCCTTCCACTTATGCTTTACCTTCTATTTATCCTTCTACTTACAGTA
  AACAGGCTGCATTCCAAAATGGCTTCAATCCAAGAATGCCCACTTTTCCATCTACAGAACCTATATATTT
  AAGTCTTCCGGGACAATCTCCATATTTCTCATATCCTTTGACACCTGCCACACCCTTTCATCCACAAGGA
  AGCTTACCTATCTATCGTCCAGTAGTCAGTACTGACATGGCAAAACTATTTGACAAAATAGCTAGTACAT
  CAGAATTTTTAAAAAATGGGAAAGCAAGGACTGATTTGGAGATAACAGATTCAAAAGTCAGCAATCTACA
  GGTATCTCCAAAGTCTGAGGATATCAGTAAATTTGACTGGTTAGACTTGGATCCTCTAAGTAAGCCTAAG
  GTGGATAATGTGGAGGTATTAGACCATGAGGAAGAGAAAAATGTTTCAAGTTTGCTAGCAAAGGATCCTT
  GGGATGCTGTTCTTCTTGAAGAGAGATCGACAGCAAATTGTCATCTTGAAAGAAAGGTGAATGGAAAATC
  CCTTTCTGTGGCAACTGTTACAAGAAGCCAGTCTTTAAATATTCGAACAACTCAGCTTGCAAAAGCCCAG
  GGCCATATATCTCAGAAAGACCCAAATGGGACCAGTAGTTTGCCAACTGGAAGTTCTCTTCTTCAAGAAG
  TTGAAGTACAGAATGAGGAGATGGCAGCTTTTTGTCGATCCATTACAAAATTGAAGACCAAATTTCCATA
  TACCAATCACCGCACAAACCCAGGCTATTTGTTAAGTCCAGTCACAGCGCAAAGAAACATATGCGGAGAA
  AATGCTAGTGTGAAGGTCTCCATTGACATTGAAGGATTTCAGCTACCAGTTACTTTTACGTGTGATGTGA
  GTTCTACTGTAGAAATCATTATAATGCAAGCCCTTTGCTGGGTACATGATGACTTGAATCAAGTAGATGT
  TGGCAGCTATGTTCTAAAAGTTTGTGGTCAAGAGGAAGTGCTGCAGAATAATCATTGCCTTGGAAGTCAT
  GAGCATATTCAAAACTGTCGAAAATGGGACACAGAAATTAGACTACAACTCTTGACCTTCAGTGCAATGT
  GTCAAAATCTGGCCCGAACAGCAGAAGATGATGAAACACCCGTGGATTTAAACAAACACCTGTATCAAAT
  AGAAAAACCTTGCAAAGAAGCCATGACGAGACACCCTGTTGAAGAACTCTTAGATTCTTATCACAACCAA
  GTAGAACTGGCTCTTCAAATTGAAAACCAACACCGAGCAGTAGATCAAGTAATTAAAGCTGTAAGAAAAA
  TCTGTAGTGCTTTAGATGGTGTCGAGACTCTTGCCATTACAGAATCAGTAAAGAAGCTAAAGAGAGCAGT
  TAATCTTCCAAGGAGTAAAACTGCTGATGTGACTTCTTTGTTTGGAGGAGAAGACACTAGCAGGAGTTCA
  ACTAGGGGCTCACTTAATCCTGAAAATCCTGTTCAAGTAAGCATAAACCAATTAACTGCAGCAATTTATG
  ATCTTCTCAGACTCCATGCAAATTCTGGTAGGAGTCCTACAGACTGTGCCCAAAGTAGCAAGAGTGTCAA
  GGAAGCATGGACTACAACAGAGCAGCTCCAGTTTACTATTTTTGCTGCTCATGGAATTTCAAGTAATTGG
  GTATCAAATTATGAAAAATACTACTTGATATGTTCACTGTCTCACAATGGAAAGGATCTTTTTAAACCTA
  TTCAATCAAAGAAGGTTGGCACTTACAAGAATTTCTTCTATCTTATTAAATGGGATGAACTAATCATTTT
  TCCTATCCAGATATCACAATTGCCATTAGAATCAGTTCTTCACCTTACTCTTTTTGGAATTTTAAATCAG
  AGCAGTGGAAGTTCCCCTGATTCTAATAAGCAGAGAAAGGGACCAGAAGCTTTGGGCAAAGTTTCTTTAC
  CTCTTTTTGACTTTAAACGGTTTTTAACATGTGGAACTAAACTTCTATATCTTTGGACTTCATCACATAC
  AAATTCTGTTCCTGGAACAGTTACCAAAAAAGGATATGTCATGGAAAGAATAGTGCTACAGGTTGATTTT
  CCTTCTCCTGCATTTGATATTATTTATACAACTCCTCAAGTTGACAGAAGCATTATACAGCAACATAACT
  TAGAAACACTAGAGAATGATATAAAAGGGAAACTTCTTGATATTCTTCATAAAGACTCATCACTTGGACT
  TTCTAAAGAAGATAAAGCTTTTTTATGGGAGAAACGTTATTATTGCTTCAAACACCCAAATTGTCTTCCT
  AAAATATTAGCAAGCGCCCCAAACTGGAAATGGGTTAATCTTGCCAAAACTTACTCATTGCTTCACCAGT
  GGCCTGCATTGTACCCACTAATTGCATTGGAACTTCTTGATTCAAAATTTGCTGATCAGGAAGTAAGATC
  CCTAGCTGTGACCTGGATTGAGGCCATTAGTGATGATGAGCTAACAGATCTTCTTCCACAGTTTGTACAA
  GCTTTGAAATATGAAATTTACTTGAATAGTTCATTAGTGCAATTCCTTTTGTCCAGGGCATTGGGAAATA
  TCCAGATAGCACACAATTTATATTGGCTTCTCAAAGATGCCCTGCATGATGTACAGTTTAGTACCCGATA
  CGAACATGTTTTGGGTGCTCTCCTGTCAGTAGGAGGAAAACGACTTAGAGAAGAACTTCTAAAACAGACG
  AAACTTGTACAGCTTTTAGGAGGAGTAGCAGAAAAAGTAAGGCAGGCTAGTGGATCAGCCAGACAGGTTG
  TTCTCCAAAGAAGTATGGAACGAGTACAGTCCTTTTTTCAGAAAAATAAATGCCGTCTCCCTCTCAAGCC
  AAGTCTAGTGGCAAAAGAATTAAATATTAAGTCGTGTTCCTTCTTCAGTTCTAATGCTGTCCCCCTAAAA
  GTCACAATGGTGAATGCTGACCCTATGGGAGAAGAAATTAATGTCATGTTTAAGGTTGGTGAAGATCTTC
  GGCAAGATATGTTAGCTTTACAGATGATAAAGATTATGGATAAGATCTGGCTTAAAGAAGGACTAGATCT
  GAGGATGGTAATTTTCAAATGTCTCTCAACTGGCAGAGATCGAGGCATGGTGGAGCTGGTTCCTGCTTCC
  GATACCCTCAGGAAAATCCAAGTGGAATATGGTGTGACAGGATCCTTTAAAGATAAACCACTTGCAGAGT
  GGCTAAGGAAATACAATCCCTCTGAAGAAGAATATGAAAAGGCTTCAGAGAACTTTATCTATTCCTGTGC
  TGGATGCTGTGTAGCCACCTATGTTTTAGGCATCTGTGATCGACACAATGACAATATAATGCTTCGAAGC
  ACGGGACACATGTTTCACATTGACTTTGGAAAGTTTTTGGGACATGCACAGATGTTTGGCAGCTTCAAAA
  GGGATCGGGCTCCTTTTGTGCTGACCTCTGATATGGCATATGTCATTAATGGGGGTGAAAAGCCCACCAT
  TCGTTTTCAGTTGTTTGTGGACCTCTGCTGTCAGGCCTACAACTTGATAAGAAAGCAGACAAACCTTTTT
  CTTAACCTCCTTTCACTGATGATTCCTTCAGGGTTACCAGAACTTACAAGTATTCAAGATTTGAAATACG
  TTAGAGATGCACTTCAACCCCAAACTACAGACGCAGAAGCTACAATTTTCTTTACTAGGCTTATTGAATC
  AAGTTTGGGAAGCATTGCCACAAAGTTTAACTTCTTCATTCACAACCTTGCTCAGCTTCGTTTTTCTGGT
  CTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACTCCTTTAGACAAGATGGTCGAA
  TCAAGGAAGTCTCTGTTTTTACATATCATAAGAAATACAACCCAGATAAACATTATATTTATGTAGTCCG
  AATTTTGAGGGAAGGACAGATTGAACCATCATTTGTCTTCCGAACATTTGACGAATTTCAGGAACTTCAC
  AATAAGCTCAGTATTATTTTTCCACTTTGGAAGTTACCAGGCTTTCCTAATAGGATGGTTCTAGGAAGAA
  CACACATAAAAGATGTAGCAGCCAAAAGGAAAATTGAGTTAAACAGTTACTTACAGAGTTTGATGAATGC
  TTCAACGGATGTAGCAGAGTGTGATCTTGTTTGTACTTTCTTCCACCCTTTACTTCGTGATGAGAAAGCT
  GAAGGGATAGCTAGGTCTGCAGATGCAGGTTCCTTCAGTCCTACTCCAGGCCAAATAGGAGGAGCTGTGA
  AATTATCCATCTCTTACCGAAATGGTACTCTTTTCATCATGGTGATGCATATCAAAGATCTTGTTACTGA
  AGATGGAGCTGACCCAAATCCATATGTCAAAACATACCTACTTCCAGATAACCACAAAACATCCAAACGT
  AAAACCAAAATTTCACGAAAAACGAGGAATCCGACATTCAATGAAATGCTTGTATACAGTGGATATAGCA
  AAGAAACCCTAAGACAGCGAGAACTTCAACTAAGTGTACTCAGTGCAGAATCTCTGCGGGAGAATTTTTT
  CTTGGGTGGAGTAACCCTGCCTTTGAAAGATTTCAACTTGAGCAAAGAGACGGTTAAATGGTATCAGCTG
  ACTGCGGCAACATACTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222192 representing NM_002645
  Red=Cloning site Green=Tags(s)
  
  MAQISSNSGFKECPSSHPEPTRAKDVDKEEALQMEAEALAKLQKDRQVTDNQRGFELSSSTRKKAQVYNK
  QDYDLMVFPESDSQKRALDIDVEKLTQAELEKLLLDDSFETKKTPVLPVTPILSPSFSAQLYFRPTIQRG
  QWPPGLPGPSTYALPSIYPSTYSKQAAFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATPFHPQG
  SLPIYRPVVSTDMAKLFDKIASTSEFLKNGKARTDLEITDSKVSNLQVSPKSEDISKFDWLDLDPLSKPK
  VDNVEVLDHEEEKNVSSLLAKDPWDAVLLEERSTANCHLERKVNGKSLSVATVTRSQSLNIRTTQLAKAQ
  GHISQKDPNGTSSLPTGSSLLQEVEVQNEEMAAFCRSITKLKTKFPYTNHRTNPGYLLSPVTAQRNICGE
  NASVKVSIDIEGFQLPVTFTCDVSSTVEIIIMQALCWVHDDLNQVDVGSYVLKVCGQEEVLQNNHCLGSH
  EHIQNCRKWDTEIRLQLLTFSAMCQNLARTAEDDETPVDLNKHLYQIEKPCKEAMTRHPVEELLDSYHNQ
  VELALQIENQHRAVDQVIKAVRKICSALDGVETLAITESVKKLKRAVNLPRSKTADVTSLFGGEDTSRSS
  TRGSLNPENPVQVSINQLTAAIYDLLRLHANSGRSPTDCAQSSKSVKEAWTTTEQLQFTIFAAHGISSNW
  VSNYEKYYLICSLSHNGKDLFKPIQSKKVGTYKNFFYLIKWDELIIFPIQISQLPLESVLHLTLFGILNQ
  SSGSSPDSNKQRKGPEALGKVSLPLFDFKRFLTCGTKLLYLWTSSHTNSVPGTVTKKGYVMERIVLQVDF
  PSPAFDIIYTTPQVDRSIIQQHNLETLENDIKGKLLDILHKDSSLGLSKEDKAFLWEKRYYCFKHPNCLP
  KILASAPNWKWVNLAKTYSLLHQWPALYPLIALELLDSKFADQEVRSLAVTWIEAISDDELTDLLPQFVQ
  ALKYEIYLNSSLVQFLLSRALGNIQIAHNLYWLLKDALHDVQFSTRYEHVLGALLSVGGKRLREELLKQT
  KLVQLLGGVAEKVRQASGSARQVVLQRSMERVQSFFQKNKCRLPLKPSLVAKELNIKSCSFFSSNAVPLK
  VTMVNADPMGEEINVMFKVGEDLRQDMLALQMIKIMDKIWLKEGLDLRMVIFKCLSTGRDRGMVELVPAS
  DTLRKIQVEYGVTGSFKDKPLAEWLRKYNPSEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLRS
  TGHMFHIDFGKFLGHAQMFGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQAYNLIRKQTNLF
  LNLLSLMIPSGLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIESSLGSIATKFNFFIHNLAQLRFSG
  LPSNDEPILSFSPKTYSFRQDGRIKEVSVFTYHKKYNPDKHYIYVVRILREGQIEPSFVFRTFDEFQELH
  NKLSIIFPLWKLPGFPNRMVLGRTHIKDVAAKRKIELNSYLQSLMNASTDVAECDLVCTFFHPLLRDEKA
  EGIARSADAGSFSPTPGQIGGAVKLSISYRNGTLFIMVMHIKDLVTEDGADPNPYVKTYLLPDNHKTSKR
  KTKISRKTRNPTFNEMLVYSGYSKETLRQRELQLSVLSAESLRENFFLGGVTLPLKDFNLSKETVKWYQL
  TAATYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002645
• ORF Size: 5058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002645.4
• RefSeq Size: 5061 bp
• RefSeq ORF: 5061 bp
• Locus ID: 5286
• UniProt ID: O00443
• Cytogenetics: 11p15.1
• Domains: C2, PI3K_rbd, PI3_PI4_kinase, PI3Ka, PX, PI3K_C2
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 190.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008]