MAML2 (NM_032427) Human Tagged ORF Clone

CAT#: RC222077

MAML2 (Myc-DDK-tagged)-Human mastermind-like 2 (Drosophila) (MAML2)

Product Images

Circular map for RC222077

• 

  Western blot validation of overexpression lysate (Cat# LY410122) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222077 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MAML2
• Synonyms: MAM-3; MAM2; MAM3; MLL-MAML2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222077 representing NM_032427
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACACAGCGCCCCCGCAGGCCCCCGCAGGAGGGCTAGGGGGGGCCTCTGGGGCGGGGCTCCTTG
  GAGGGGGCTCAGTCACCCCGAGAGTGCACAGTGCTATCGTGGAGCGCCTCCGGGCTCGGATCGCTGTCTG
  CCGCCAACACCACCTGAGCTGTGAAGGACGATATGAACGAGGTAGGGCCGAGAGCTCAGACCGGGAAAGA
  GAAAGCACCTTGCAGCTCCTGAGCCTTGTACAGCATGGCCAGGGGGCAAGGAAAGCTGGCAAACACACCA
  AGGCCACCGCCACTGCTGCCACCACTACAGCCCCTCCACCGCCCCCTGCTGCCCCTCCTGCGGCCTCCCA
  AGCAGCAGCAACAGCAGCCCCACCGCCCCCACCAGACTATCACCATCACCACCAGCAGCACCTGCTGAAC
  AGTAGCAATAATGGTGGCAGTGGTGGGATAAACGGAGAGCAGCAGCCGCCCGCTTCAACCCCAGGGGACC
  AGAGGAACTCAGCCCTGATTGCGCTCCAGGGTTCCTTGAAAAGAAAACAGGTAGTTAACCTATCTCCTGC
  CAACAGCAAGCGACCCAATGGCTTTGTGGACAACTCATTTCTTGATATCAAAAGAATTCGTGTTGGGGAG
  AATCTCTCTGCAGGACAAGGTGGCCTCCAAATAAACAATGGACAAAGTCAGATTATGTCAGGGACCTTGC
  CTATGAGCCAAGCACCCCTGCGAAAGACTAACACTCTGCCATCCCATACACATTCTCCTGGCAATGGCCT
  GTTTAACATGGGCTTAAAGGAGGTAAAGAAGGAGCCAGGAGAGACTCTGTCTTGCAGTAAGCACATGGAT
  GGCCAAATGACCCAAGAGAATATTTTTCCTAATAGGTACGGAGACGACCCTGGAGAACAACTGATGGATC
  CTGAGCTGCAGGAACTGTTCAATGAACTGACCAACATATCTGTGCCTCCCATGAGTGACCTTGAACTGGA
  GAACATGATCAATGCCACCATAAAGCAGGATGACCCATTTAACATTGACTTGGGTCAGCAAAGCCAGAGG
  AGCACACCTAGGCCCTCCTTACCCATGGAGAAAATAGTGATCAAAAGTGAATACTCACCGGGCTTGACTC
  AGGGCCCCTCAGGCTCTCCTCAGCTGAGGCCCCCATCAGCTGGCCCCGCATTCTCCATGGCCAACTCTGC
  CCTCTCCACTTCGTCTCCAATCCCTTCAGTCCCTCAGAGCCAGGCTCAGCCTCAGACAGGCTCCGGAGCA
  AGCCGGGCCTTGCCAAGCTGGCAGGAAGTATCCCATGCCCAGCAGCTCAAACAGATAGCTGCTAATCGTC
  AGCAGCATGCCCGGATGCAGCAGCACCAGCAGCAGCACCAGCCTACCAACTGGTCAGCCTTGCCCTCCTC
  TGCTGGACCATCACCAGGTCCATTTGGGCAGGAGAAAATCCCCAGCCCTTCTTTTGGTCAGCAGACATTC
  AGCCCACAGAGCTCCCCCATGCCTGGGGTAGCTGGCGGCAGCGGCCAGTCGAAAGTAATGGCTAACTACA
  TGTACAAGGCCGGCCCCTCAGCCCAGGGTGGGCACCTAGATGTCCTCATGCAGCAAAAGCCTCAGGATCT
  CAGTCGAAGTTTTATTAACAACCCGCACCCAGCCATGGAGCCCCGTCAGGGCAACACCAAGCCTTTGTTT
  CATTTTAACTCAGATCAAGCGAACCAGCAGATGCCTTCTGTTTTGCCTTCCCAGAACAAGCCTTCTCTCC
  TACACTACACCCAACAGCAACAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCA
  GCAGCAGCAACAGCAACAGCAACAGCAACAGCAGAGTTCAATTTCAGCTCAACAACAGCAACAGCAGCAG
  AGCTCAATTTCAGCCCAACAGCAGCAGCAGCAGCAACAACAGCAGCAGCAGCAGCAACAACAACAGCAAC
  AACAGCAGCAGCAGCAGCAGCAACAACCATCTTCTCAGCCTGCCCAATCTCTACCAAGCCAGCCTTTGCT
  AAGGTCACCTTTGCCACTTCAGCAAAAGCTCCTACTTCAGCAAATGCAGAATCAGCCCATTGCAGGAATG
  GGATACCAAGTCTCCCAACAACAGAGACAGGATCAACACTCTGTGGTAGGCCAGAACACAGGCCCCAGTC
  CAAGTCCTAACCCCTGCTCAAATCCAAACACTGGAAGTGGTTACATGAACTCCCAGCAATCACTGTTGAA
  TCAGCAATTGATGGGAAAGAAGCAGACTCTACAGAGGCAGATCATGGAGCAGAAACAGCAACTTCTTCTC
  CAGCAGCAGATGCTGGCTGACGCGGAGAAAATTGCTCCACAAGATCAGATAAACCGACATTTGTCAAGGC
  CACCTCCAGATTATAAAGACCAAAGAAGAAATGTGGGCAATATGCAACCAACTGCTCAGTATTCTGGTGG
  CTCATCCACAATAAGCTTAAACTCTAACCAGGCTTTGGCAAACCCAGTTTCAACACACACCATTTTAACT
  CCCAATTCCAGCCTCCTGTCTACTTCTCACGGGACAAGAATGCCATCATTATCTACAGCAGTTCAGAATA
  TGGGGATGTATGGAAATCTGCCTTGTAATCAACCTAACACATACAGTGTCACTTCAGGAATGAATCAATT
  GACCCAACAGAGAAACCCAAAGCAATTGTTAGCAAATCAAAACAACCCTATGATGCCACGGCCACCTACC
  TTAGGGCCAAGTAATAATAACAATGTAGCCACTTTTGGAGCTGGATCTGTTGGTAATTCACAACAATTGA
  GACCAAATTTAACCCATAGTATGGCAAGCATGCCACCACAGAGAACATCAAACGTAATGATCACATCCAA
  CACAACTGCACCAAACTGGGCCTCTCAAGAAGGAACAAGCAAACAGCAAGAAGCCCTGACGTCTGCAGGA
  GTCCGCTTCCCCACAGGTACACCTGCAGCCTATACCCCAAATCAGTCACTGCAACAGGCAGTAGGTAGCC
  AGCAATTTTCCCAGAGGGCAGTGGCTCCTCCTAACCAGTTAACACCAGCAGTGCAAATGAGACCCATGAA
  CCAAATGAGCCAAACACTAAATGGGCAAACCATGGGTCCCCTCAGGGGTCTGAATCTCAGACCCAATCAG
  CTAAGCACACAGATTTTGCCTAATTTGAATCAGTCAGGAACAGGGTTGAATCAGTCGAGGACGGGCATCA
  ACCAGCCACCATCCCTGACGCCCAGCAATTTTCCTTCACCCAACCAAAGTTCCAGGGCTTTTCAAGGAAC
  TGACCACAGCAGTGACTTAGCTTTTGACTTCCTCAGCCAACAAAATGATAACATGGGCCCTGCCCTAAAC
  AGTGATGCTGATTTCATTGATTCTTTATTGAAGACAGAGCCTGGTAATGATGACTGGATGAAAGACATCA
  ATCTTGATGAAATCTTGGGGAACAATTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222077 representing NM_032427
  Red=Cloning site Green=Tags(s)
  
  MGDTAPPQAPAGGLGGASGAGLLGGGSVTPRVHSAIVERLRARIAVCRQHHLSCEGRYERGRAESSDRER
  ESTLQLLSLVQHGQGARKAGKHTKATATAATTTAPPPPPAAPPAASQAAATAAPPPPPDYHHHHQQHLLN
  SSNNGGSGGINGEQQPPASTPGDQRNSALIALQGSLKRKQVVNLSPANSKRPNGFVDNSFLDIKRIRVGE
  NLSAGQGGLQINNGQSQIMSGTLPMSQAPLRKTNTLPSHTHSPGNGLFNMGLKEVKKEPGETLSCSKHMD
  GQMTQENIFPNRYGDDPGEQLMDPELQELFNELTNISVPPMSDLELENMINATIKQDDPFNIDLGQQSQR
  STPRPSLPMEKIVIKSEYSPGLTQGPSGSPQLRPPSAGPAFSMANSALSTSSPIPSVPQSQAQPQTGSGA
  SRALPSWQEVSHAQQLKQIAANRQQHARMQQHQQQHQPTNWSALPSSAGPSPGPFGQEKIPSPSFGQQTF
  SPQSSPMPGVAGGSGQSKVMANYMYKAGPSAQGGHLDVLMQQKPQDLSRSFINNPHPAMEPRQGNTKPLF
  HFNSDQANQQMPSVLPSQNKPSLLHYTQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSSISAQQQQQQQ
  SSISAQQQQQQQQQQQQQQQQQQQQQQQQQQQPSSQPAQSLPSQPLLRSPLPLQQKLLLQQMQNQPIAGM
  GYQVSQQQRQDQHSVVGQNTGPSPSPNPCSNPNTGSGYMNSQQSLLNQQLMGKKQTLQRQIMEQKQQLLL
  QQQMLADAEKIAPQDQINRHLSRPPPDYKDQRRNVGNMQPTAQYSGGSSTISLNSNQALANPVSTHTILT
  PNSSLLSTSHGTRMPSLSTAVQNMGMYGNLPCNQPNTYSVTSGMNQLTQQRNPKQLLANQNNPMMPRPPT
  LGPSNNNNVATFGAGSVGNSQQLRPNLTHSMASMPPQRTSNVMITSNTTAPNWASQEGTSKQQEALTSAG
  VRFPTGTPAAYTPNQSLQQAVGSQQFSQRAVAPPNQLTPAVQMRPMNQMSQTLNGQTMGPLRGLNLRPNQ
  LSTQILPNLNQSGTGLNQSRTGINQPPSLTPSNFPSPNQSSRAFQGTDHSSDLAFDFLSQQNDNMGPALN
  SDADFIDSLLKTEPGNDDWMKDINLDEILGNNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032427
• ORF Size: 3459 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032427.4
• RefSeq Size: 5428 bp
• RefSeq ORF: 3471 bp
• Locus ID: 84441
• UniProt ID: Q8IZL2
• Cytogenetics: 11q21
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Notch signaling pathway
• MW: 125 kDa
• Gene Summary: The protein encoded by this gene is a member of the Mastermind-like family of proteins. All family members are proline and glutamine-rich, and contain a conserved basic domain that binds the ankyrin repeat domain of the intracellular domain of the Notch receptors (ICN1-4) in their N-terminus, and a transcriptional activation domain in their C-terminus. This protein binds to an extended groove that is formed by the interaction of CBF1, Suppressor of Hairless, LAG-1 (CSL) with ICN, and positively regulates Notch signaling. High levels of expression of this gene have been observed in several B cell-derived lymphomas. Translocations resulting in fusion proteins with both CRTC1 and CRTC3 have been implicated in the development of mucoepidermoid carcinomas, while a translocation event with CXCR4 has been linked with chronic lymphocytic leukemia (CLL). Copy number variation in the polyglutamine tract has been observed. [provided by RefSeq, Jan 2015]