MAML2 (NM_032427) Human Tagged ORF Clone

CAT#: RG222077

MAML2 (tGFP-tagged) - Human mastermind-like 2 (Drosophila) (MAML2)

Product Images

Circular map for RG222077

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MAML2
• Synonyms: MAM-3; MAM2; MAM3; MLL-MAML2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222077 representing NM_032427
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGACACAGCGCCCCCGCAGGCCCCCGCAGGAGGGCTAGGGGGGGCCTCTGGGGCGGGGCTCCTTG
  GAGGGGGCTCAGTCACCCCGAGAGTGCACAGTGCTATCGTGGAGCGCCTCCGGGCTCGGATCGCTGTCTG
  CCGCCAACACCACCTGAGCTGTGAAGGACGATATGAACGAGGTAGGGCCGAGAGCTCAGACCGGGAAAGA
  GAAAGCACCTTGCAGCTCCTGAGCCTTGTACAGCATGGCCAGGGGGCAAGGAAAGCTGGCAAACACACCA
  AGGCCACCGCCACTGCTGCCACCACTACAGCCCCTCCACCGCCCCCTGCTGCCCCTCCTGCGGCCTCCCA
  AGCAGCAGCAACAGCAGCCCCACCGCCCCCACCAGACTATCACCATCACCACCAGCAGCACCTGCTGAAC
  AGTAGCAATAATGGTGGCAGTGGTGGGATAAACGGAGAGCAGCAGCCGCCCGCTTCAACCCCAGGGGACC
  AGAGGAACTCAGCCCTGATTGCGCTCCAGGGTTCCTTGAAAAGAAAACAGGTAGTTAACCTATCTCCTGC
  CAACAGCAAGCGACCCAATGGCTTTGTGGACAACTCATTTCTTGATATCAAAAGAATTCGTGTTGGGGAG
  AATCTCTCTGCAGGACAAGGTGGCCTCCAAATAAACAATGGACAAAGTCAGATTATGTCAGGGACCTTGC
  CTATGAGCCAAGCACCCCTGCGAAAGACTAACACTCTGCCATCCCATACACATTCTCCTGGCAATGGCCT
  GTTTAACATGGGCTTAAAGGAGGTAAAGAAGGAGCCAGGAGAGACTCTGTCTTGCAGTAAGCACATGGAT
  GGCCAAATGACCCAAGAGAATATTTTTCCTAATAGGTACGGAGACGACCCTGGAGAACAACTGATGGATC
  CTGAGCTGCAGGAACTGTTCAATGAACTGACCAACATATCTGTGCCTCCCATGAGTGACCTTGAACTGGA
  GAACATGATCAATGCCACCATAAAGCAGGATGACCCATTTAACATTGACTTGGGTCAGCAAAGCCAGAGG
  AGCACACCTAGGCCCTCCTTACCCATGGAGAAAATAGTGATCAAAAGTGAATACTCACCGGGCTTGACTC
  AGGGCCCCTCAGGCTCTCCTCAGCTGAGGCCCCCATCAGCTGGCCCCGCATTCTCCATGGCCAACTCTGC
  CCTCTCCACTTCGTCTCCAATCCCTTCAGTCCCTCAGAGCCAGGCTCAGCCTCAGACAGGCTCCGGAGCA
  AGCCGGGCCTTGCCAAGCTGGCAGGAAGTATCCCATGCCCAGCAGCTCAAACAGATAGCTGCTAATCGTC
  AGCAGCATGCCCGGATGCAGCAGCACCAGCAGCAGCACCAGCCTACCAACTGGTCAGCCTTGCCCTCTTC
  TGCTGGACCATCACCAGGTCCATTTGGGCAGGAGAAAATCCCCAGCCCTTCTTTTGGTCAGCAGACATTC
  AGCCCACAGAGCTCCCCCATGCCTGGGGTAGCTGGCGGCAGCGGCCAGTCGAAAGTAATGGCTAACTACA
  TGTACAAGGCCGGCCCCTCAGCCCAGGGTGGGCACCTAGATGTCCTCATGCAGCAAAAGCCTCAGGATCT
  CAGTCGAAGTTTTATTAACAACCCGCACCCAGCCATGGAGCCCCGTCAGGGCAACACCAAGCCTTTGTTT
  CATTTTAACTCAGATCAAGCGAACCAGCAGATGCCTTCTGTTTTGCCTTCCCAGAACAAGCCTTCTCTCC
  TACACTACACCCAACAGCAACAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCA
  ACAGCAGCAGCAGCAGCAACAGCAACAGCAACAGCAACAGCAGAGTTCAATTTCAGCTCAACAACAGCAA
  CAGCAGCAGAGCTCAATTTCAGCCCAACAGCAGCAGCAGCAGCAACAACAGCAGCAGCAGCAGCAACAAC
  AACAGCAACAACAGCAGCAGCAGCAGCAGCAACAACCATCTTCTCAGCCTGCCCAATCTCTACCAAGCCA
  GCCTTTGCTAAGGTCACCTTTGCCACTTCAGCAAAAGCTCCTACTTCAGCAAATGCAGAATCAGCCCATT
  GCAGGAATGGGATACCAAGTCTCCCAACAACAGAGACAGGATCAACACTCTGTGGTAGGCCAGAACACAG
  GCCCCAGTCCAAGTCCTAACCCCTGCTCAAATCCAAACACTGGAAGTGGTTACATGAACTCCCAGCAATC
  ACTGTTGAATCAGCAATTGATGGGAAAGAAGCAGACTCTACAGAGGCAGATCATGGAGCAGAAACAGCAA
  CTTCTTCTCCAGCAGCAGATGCTGGCTGACGCGGAGAAAATTGCTCCACAAGATCAGATAAACCGACATT
  TGTCAAGGCCACCTCCAGATTATAAAGACCAAAGAAGAAATGTGGGCAATATGCAACCAACTGCTCAGTA
  TTCTGGTGGCTCATCCACAATAAGCTTAAACTCTAACCAGGCTTTGGCAAACCCAGTTTCAACACACACC
  ATTTTAACTCCCAATTCCAGCCTCCTGTCTACTTCTCACGGGACAAGAATGCCATCATTATCTACAGCAG
  TTCAGAATATGGGGATGTATGGAAATCTGCCTTGTAATCAACCTAACACATACAGTGTCACTTCAGGAAT
  GAATCAATTGACCCAACAGAGAAACCCAAAGCAATTGTTAGCAAATCAAAACAACCCTATGATGCCACGG
  CCACCTACCTTAGGGCCAAGTAATAATAACAATGTAGCCACTTTTGGAGCTGGATCTGTTGGTAATTCAC
  AACAATTGAGACCAAATTTAACCCATAGTATGGCAAGCATGCCACCACAGAGAACATCAAACGTAATGAT
  CACATCCAACACAACTGCACCAAACTGGGCCTCTCAAGAAGGAACAAGCAAACAGCAAGAAGCCCTGACG
  TCTGCAGGAGTCCGCTTCCCCACAGGTACACCTGCAGCCTATACCCCAAATCAGTCACTGCAACAGGCAG
  TAGGTAGCCAGCAATTTTCCCAGAGGGCAGTGGCTCCTCCTAACCAGTTAACACCAGCAGTGCAAATGAG
  ACCCATGAACCAAATGAGCCAAACACTAAATGGGCAAACCATGGGTCCCCTCAGGGGTCTGAATCTCAGA
  CCCAATCAGCTAAGCACACAGATTTTGCCTAATTTGAATCAGTCAGGAACAGGGTTGAATCAGTCGAGGA
  CGGGCATCAACCAGCCACCATCCCTGACGCCCAGCAATTTTCCTTCACCCAACCAAAGTTCCAGGGCTTT
  TCAAGGAACTGACCACAGCAGTGACTTAGCTTTTGACTTCCTCAGCCAACAAAATGATAACATGGGCCCT
  GCCCTAAACAGTGATGCTGATTTCATTGATTCTTTATTGAAGACAGAGCCTGGTAATGATGACTGGATGA
  AAGACATCAATCTTGATGAAATCTTGGGGAACAATTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222077 representing NM_032427
  Red=Cloning site Green=Tags(s)
  
  MGDTAPPQAPAGGLGGASGAGLLGGGSVTPRVHSAIVERLRARIAVCRQHHLSCEGRYERGRAESSDRER
  ESTLQLLSLVQHGQGARKAGKHTKATATAATTTAPPPPPAAPPAASQAAATAAPPPPPDYHHHHQQHLLN
  SSNNGGSGGINGEQQPPASTPGDQRNSALIALQGSLKRKQVVNLSPANSKRPNGFVDNSFLDIKRIRVGE
  NLSAGQGGLQINNGQSQIMSGTLPMSQAPLRKTNTLPSHTHSPGNGLFNMGLKEVKKEPGETLSCSKHMD
  GQMTQENIFPNRYGDDPGEQLMDPELQELFNELTNISVPPMSDLELENMINATIKQDDPFNIDLGQQSQR
  STPRPSLPMEKIVIKSEYSPGLTQGPSGSPQLRPPSAGPAFSMANSALSTSSPIPSVPQSQAQPQTGSGA
  SRALPSWQEVSHAQQLKQIAANRQQHARMQQHQQQHQPTNWSALPSSAGPSPGPFGQEKIPSPSFGQQTF
  SPQSSPMPGVAGGSGQSKVMANYMYKAGPSAQGGHLDVLMQQKPQDLSRSFINNPHPAMEPRQGNTKPLF
  HFNSDQANQQMPSVLPSQNKPSLLHYTQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQSSISAQQQQ
  QQQSSISAQQQQQQQQQQQQQQQQQQQQQQQQQQQPSSQPAQSLPSQPLLRSPLPLQQKLLLQQMQNQPI
  AGMGYQVSQQQRQDQHSVVGQNTGPSPSPNPCSNPNTGSGYMNSQQSLLNQQLMGKKQTLQRQIMEQKQQ
  LLLQQQMLADAEKIAPQDQINRHLSRPPPDYKDQRRNVGNMQPTAQYSGGSSTISLNSNQALANPVSTHT
  ILTPNSSLLSTSHGTRMPSLSTAVQNMGMYGNLPCNQPNTYSVTSGMNQLTQQRNPKQLLANQNNPMMPR
  PPTLGPSNNNNVATFGAGSVGNSQQLRPNLTHSMASMPPQRTSNVMITSNTTAPNWASQEGTSKQQEALT
  SAGVRFPTGTPAAYTPNQSLQQAVGSQQFSQRAVAPPNQLTPAVQMRPMNQMSQTLNGQTMGPLRGLNLR
  PNQLSTQILPNLNQSGTGLNQSRTGINQPPSLTPSNFPSPNQSSRAFQGTDHSSDLAFDFLSQQNDNMGP
  ALNSDADFIDSLLKTEPGNDDWMKDINLDEILGNNS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032427
• ORF Size: 3468 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032427.4
• RefSeq Size: 5428 bp
• RefSeq ORF: 3471 bp
• Locus ID: 84441
• UniProt ID: Q8IZL2
• Cytogenetics: 11q21
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Notch signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the Mastermind-like family of proteins. All family members are proline and glutamine-rich, and contain a conserved basic domain that binds the ankyrin repeat domain of the intracellular domain of the Notch receptors (ICN1-4) in their N-terminus, and a transcriptional activation domain in their C-terminus. This protein binds to an extended groove that is formed by the interaction of CBF1, Suppressor of Hairless, LAG-1 (CSL) with ICN, and positively regulates Notch signaling. High levels of expression of this gene have been observed in several B cell-derived lymphomas. Translocations resulting in fusion proteins with both CRTC1 and CRTC3 have been implicated in the development of mucoepidermoid carcinomas, while a translocation event with CXCR4 has been linked with chronic lymphocytic leukemia (CLL). Copy number variation in the polyglutamine tract has been observed. [provided by RefSeq, Jan 2015]