DNMT3B (NM_175850) Human Tagged ORF Clone

CAT#: RC222068

DNMT3B (Myc-DDK-tagged)-Human DNA (cytosine-5-)-methyltransferase 3 beta (DNMT3B), transcript variant 6

Product Images

Circular map for RC222068

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DNMT3B
• Synonyms: ICF; ICF1; M.HsaIIIB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222068 representing NM_175850
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCAAGTCCTGAGCCTCCAAGCTTGGAAAGCATGAAGGGAGACACCAGGCATCTCAATGGAGAGG
  AGGACGCCGGCGGGAGGGAAGACTCGATCCTCGTCAACGGGGCCTGCAGCGACCAGTCCTCCGACTCGCC
  CCCAATCCTGGAGGCTATCCGCACCCCGGAGATCAGAGGCCGAAGATCAAGCTCGCGACTCTCCAAGAGG
  GAGGTGTCCAGTCTGCTAAGCTACACACAGGACTTGACAGGCGATGGCGACGGGGAAGATGGGGATGGCT
  CTGACACCCCAGTCATGCCAAAGCTCTTCCGGGAAACCAGGACTCGTTCAGAAAGCCCAGCTGTCCGAAC
  TCGAAATAACAACAGTGTCTCCAGCCGGGAGAGGCACAGGCCTTCCCCACGTTCCACCCGAGGCCGGCAG
  GGCCGCAACCATGTGGACGAGTCCCCCGTGGAGTTCCCGGCTACCAGGTCCCTGAGACGGCGGGCAACAG
  CATCGGCAGGAACGCCATGGCCGTCCCCTCCCAGCTCTTACCTTACCATCGACCTCACAGACGACACAGA
  GGACACACATGGGACGCCCCAGAGCAGCAGTACCCCCTACGCCCGCCTAGCCCAGGACAGCCAGCAGGGG
  GGCATGGAGTCCCCGCAGGTGGAGGCAGACAGTGGAGATGGAGACAGTTCAGAGTATCAGGATGGGAAGG
  AGTTTGGAATAGGGGACCTCGTGTGGGGAAAGATCAAGGGCTTCTCCTGGTGGCCCGCCATGGTGGTGTC
  TTGGAAGGCCACCTCCAAGCGACAGGCTATGTCTGGCATGCGGTGGGTCCAGTGGTTTGGCGATGGCAAG
  TTCTCCGAGGTCTCTGCAGACAAACTGGTGGCACTGGGGCTGTTCAGCCAGCACTTTAATTTGGCCACCT
  TCAATAAGCTCGTCTCCTATCGAAAAGCCATGTACCATGCTCTGGAGAAAGCTAGGGTGCGAGCTGGCAA
  GACCTTCCCCAGCAGCCCTGGAGACTCATTGGAGGACCAGCTGAAGCCCATGTTGGAGTGGGCCCACGGG
  GGCTTCAAGCCCACTGGGATCGAGGGCCTCAAACCCAACAACACGCAACCAGAGAACAAGACTCGAAGAC
  GCACAGCTGACGACTCAGCCACCTCTGACTACTGCCCCGCACCCAAGCGCCTCAAGACAAATTGCTATAA
  CAACGGCAAAGACCGAGGGGATGAAGATCAGAGCCGAGAACAAATGGCTTCAGATGTTGCCAACAACAAG
  AGCAGCCTGGAAGATGGCTGTTTGTCTTGTGGCAGGAAAAACCCCGTGTCCTTCCACCCTCTCTTTGAGG
  GGGGGCTCTGTCAGACATGCCGGGATCGCTTCCTTGAGCTGTTTTACATGTATGATGACGATGGCTATCA
  GTCTTACTGCACTGTGTGCTGCGAGGGCCGAGAGCTGCTGCTTTGCAGCAACACGAGCTGCTGCCGGTGT
  TTCTGTGTGGAGTGCCTGGAGGTGCTGGTGGGCACAGGCACAGCGGCCGAGGCCAAGCTTCAGGAGCCCT
  GGAGCTGTTACATGTGTCTCCCGCAGCGCTGTCATGGCGTCCTGCGGCGCCGGAAGGACTGGAACGTGCG
  CCTGCAGGCCTTCTTCACCAGTGACACGGGGCTTGAATATGAAGCCCCCAAGCTGTACCCTGCCATTCCC
  GCAGCCCGAAGGCGGCCCATTCGAGTCCTGTCATTGTTTGATGGCATCGCGACAGGCTACCTAGTCCTCA
  AAGAGTTGGGCATAAAGGTAGGAAAGTACGTCGCTTCTGAAGTGTGTGAGGAGTCCATTGCTGTTGGAAC
  CGTGAAGCACGAGGGGAATATCAAATACGTGAACGACGTGAGGAACATCACAAAGAAAAATATTGAAGAA
  TGGGGCCCATTTGACTTGGTGATTGGCGGAAGCCCATGCAACGATCTCTCAAATGTGAATCCAGCCAGGA
  AAGGCCTGTATGAGGGTACAGGCCGGCTCTTCTTCGAATTTTACCACCTGCTGAATTACTCACGCCCCAA
  GGAGGGTGATGACCGGCCGTTCTTCTGGATGTTTGAGAATGTTGTAGCCATGAAGGTTGGCGACAAGAGG
  GACATCTCACGGTTCCTGGAGTGTAATCCAGTGATGATTGATGCCATCAAAGTTTCTGCTGCTCACAGGG
  CCCGATACTTCTGGGGCAACCTACCCGGGATGAACAGGCCCGTGATAGCATCAAAGAATGATAAACTCGA
  GCTGCAGGACTGCTTGGAATACAATAGGATAGCCAAGTTAAAGAAAGTACAGACAATAACCACCAAGTCG
  AACTCGATCAAACAGGGGAAAAACCAACTTTTCCCTGTTGTCATGAATGGCAAAGAAGATGTTTTGTGGT
  GCACTGAGCTCGAAAGGATCTTTGGCTTTCCTGTGCACTACACAGACGTGTCCAACATGGGCCGTGGTGC
  CCGCCAGAAGCTGCTGGGAAGGTCCTGGAGCGTGCCTGTCATCCGACACCTCTTCGCCCCTCTGAAGGAC
  TACTTTGCATGTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222068 representing NM_175850
  Red=Cloning site Green=Tags(s)
  
  MEPSPEPPSLESMKGDTRHLNGEEDAGGREDSILVNGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKR
  EVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRETRTRSESPAVRTRNNNSVSSRERHRPSPRSTRGRQ
  GRNHVDESPVEFPATRSLRRRATASAGTPWPSPPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQDSQQG
  GMESPQVEADSGDGDSSEYQDGKEFGIGDLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRWVQWFGDGK
  FSEVSADKLVALGLFSQHFNLATFNKLVSYRKAMYHALEKARVRAGKTFPSSPGDSLEDQLKPMLEWAHG
  GFKPTGIEGLKPNNTQPENKTRRRTADDSATSDYCPAPKRLKTNCYNNGKDRGDEDQSREQMASDVANNK
  SSLEDGCLSCGRKNPVSFHPLFEGGLCQTCRDRFLELFYMYDDDGYQSYCTVCCEGRELLLCSNTSCCRC
  FCVECLEVLVGTGTAAEAKLQEPWSCYMCLPQRCHGVLRRRKDWNVRLQAFFTSDTGLEYEAPKLYPAIP
  AARRRPIRVLSLFDGIATGYLVLKELGIKVGKYVASEVCEESIAVGTVKHEGNIKYVNDVRNITKKNIEE
  WGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAMKVGDKR
  DISRFLECNPVMIDAIKVSAAHRARYFWGNLPGMNRPVIASKNDKLELQDCLEYNRIAKLKKVQTITTKS
  NSIKQGKNQLFPVVMNGKEDVLWCTELERIFGFPVHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKD
  YFACE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175850
• ORF Size: 2535 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175850.2, NP_787046.1
• RefSeq Size: 4272 bp
• RefSeq ORF: 2538 bp
• Locus ID: 1789
• UniProt ID: Q9UBC3
• Cytogenetics: 20q11.21
• Protein Families: Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• MW: 94.7 kDa
• Gene Summary: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]