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COL6A6 (NM_001102608) Human Tagged ORF Clone
CAT#: RC222036
- TrueORF®
COL6A6 (Myc-DDK-tagged)-Human collagen, type VI, alpha 6 (COL6A6). Note: ORF is codon optimized
ORF Plasmid: tGFP
"NM_001102608" in other vectors (2)
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Frequently bought together (4)
DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | COL6A6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222036 ORF sequence, codon optimized.
Due to the complexity of NM_001102608, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTGCTGATCCTGTTCCTGGTCATCATCTGCAGCCACATTTCCGTCAACCAGGATAGCGGCCCAG AGTATGCCGACGTCGTTTTTTTGGTGGACTCCAGTGATAGATTGGGAAGCAAAAGCTTTCCATTTGTCAA GATGTTCATTACTAAGATGATCTCTAGTCTCCCCATTGAGGCCGACAAATACAGAGTTGCCTTGGCGCAG TACTCCGACAAACTTCATTCTGAGTTCCACCTGTCCACATTTAAGGGACGATCTCCTATGCTGAACCACC TCAGGAAAAACTTTGGTTTCATCGGAGGGTCACTGCAGATAGGAAAAGCACTTCAGGAGGCACATCGGAC CTATTTCTCCGCACCTGCCAACGGGCGCGATAAGAAGCAGTTCCCTCCAATCCTGGTAGTGCTGGCCAGC AGTGAGAGTGAGGATAATGTTGAGGAGGCTTCAAAGGCCCTGAGGAAGGACGGTGTAAAAATCATCTCCG TAGGAGTTCAGAAGGCCTCCGAGGAGAATCTGAAGGCTATGGCCACTAGCCAGTTCCACTTCAATCTGAG GACAGTCAGAGATCTGTCCATGTTTAGCCAAAATATGACACATATAATTAAGGATGTCATCAAATACAAG GAGGGAGCCGTGGACGATATTTTTGTCGAAGCTTGTCAGGGGCCCAGCATGGCTGACGTGGTGTTTCTCC TCGACATGAGTATCAACGGAAGTGAAGAAAATTTCGACTATCTGAAGGGTTTCTTGGAGGAAAGTGTCAG TGCTTTGGACATTAAAGAGAACTGTATGAGAGTGGGCCTCGTAGCTTATTCAAATGAGACTAAGGTGATC AACAGCTTGTCCATGGGAATCAACAAGTCAGAAGTGCTGCAGCATATCCAGAACCTGTCACCCAGGACCG GAAAGGCTTACACTGGTGCCGCCATTAAAAAGCTGCGGAAGGAAGTGTTCTCTGCAAGGAACGGGAGCCG GAAGAATCAGGGCGTTCCACAGATCGCCGTGTTGGTTACCCACAGGGATTCTGAAGATAACGTGACCAAG GCCGCTGTCAACCTCCGGCGAGAGGGAGTCACGATCTTCACGCTGGGAATCGAAGGAGCCTCTGATACAC AGCTGGAAAAAATAGCTAGCCATCCTGCCGAACAATATGTGAGTAAGCTCAAGACTTTTGCCGATCTCGC TGCTCATAACCAAACCTTTCTGAAAAAACTGCGAAATCAAATAACTCACACGGTGTCCGTGTTCTCAGAA CGCACAGAGACACTGAAGTCCGGGTGCGTGGATACAGAGGAAGCTGATATCTATCTGCTTATCGACGGTA GCGGTTCCACCCAGGCCACAGACTTCCACGAGATGAAGACCTTCCTTTCCGAGGTCGTCGGAATGTTCAA CATCGCACCACATAAGGTCCGGGTTGGCGCGGTCCAATATGCAGACTCCTGGGATCTGGAGTTTGAGATT AACAAGTACAGCAACAAGCAGGACCTGGGCAAGGCTATTGAGAATATTAGGCAGATGGGTGGAAACACAA ATACTGGAGCGGCTTTGAATTTTACCCTCTCCCTTCTCCAGAAGGCGAAAAAGCAGCGCGGCAACAAAGT TCCCTGTCACCTTGTGGTCCTCACCAACGGCATGTCCAAGGATAGCATCCTGGAGCCCGCAAACCGCTTG AGGGAGGAGCACATCAGAGTGTATGCCATAGGCATTAAAGAGGCGAACCAGACTCAGCTTAGAGAGATTG CCGGAGAAGAGAAACGGGTGTACTATGTACACGACTTCGATGCACTGAAAGATATCAGGAACCAGGTGGT GCAGGAAATTTGTACGGAAGAGGCATGCAAAGAAATGAAAGCCGACATCATGTTCCTGGTCGACTCTTCC GGGTCTATCGGACCAGAGAACTTCAGCAAGATGAAGACTTTCATGAAAAATCTCGTGTCCAAGTCCCAGA TTGGACCAGACCGAGTTCAGATCGGTGTTGTGCAGTTCTCCGACATTAATAAGGAAGAGTTTCAGCTGAA TAGGTTCATGTCTCAGAGTGACATTTCTAACGCTATTGACCAGATGGCCCATATAGGACAAACCACCCTG ACCGGTTCTGCCCTGTCTTTCGTGTCCCAGTATTTTTCCCCAACCAAGGGCGCCCGCCCAAATATTCGCA AGTTCCTGATACTGATCACTGATGGCGAAGCACAGGACATTGTGAAAGAGCCTGCCGTGGTTCTGAGACA AGAGGGAGTCATTATCTACTCTGTGGGAGTTTTTGGAAGTAACGTGACACAACTGGAGGAGATCTCAGGA CGGCCGGAGATGGTCTTCTACGTCGAGAACTTTGACATCCTCCAGCGGATCGAGGACGATCTGGTCTTCG GAATCTGCAGCCCACGAGAAGAATGTAAGCGAATAGAGGTGTTGGATGTCGTTTTTGTGATAGATTCTAG CGGATCAATTGACTATGACGAATATAACATAATGAAGGATTTTATGATTGGCCTCGTGAAGAAAGCCGAT GTGGGAAAGAACCAGGTCAGATTTGGTGCCCTGAAATATGCTGACGACCCCGAAGTCCTGTTCTACCTCG ATGACTTCGGGACTAAGCTGGAAGTGATCAGCGTACTCCAGAACGATCAGGCAATGGGGGGGAGTACCTA CACTGCGGAGGCCCTTGGCTTTTCTGATCACATGTTTACAGAAGCACGAGGCTCACGCTTGAACAAGGGG GTACCACAGGTGCTGATCGTTATTACTGACGGAGAATCTCATGATGCCGATAAGCTTAACGCAACTGCCA AGGCCCTGAGGGACAAGGGCATCCTGGTGCTGGCAGTAGGAATAGACGGCGCCAACCCAGTGGAATTGCT GGCTATGGCCGGCTCTTCCGACAAATATTTCTTTGTGGAGACATTCGGTGGCTTGAAGGGGATATTTTCA GACGTTACCGCATCCGTGTGTAACAGTTCCAAGGTCGACTGTGAGATTGACAAGGTCGATCTGGTCTTCC TTATGGATGGGAGTACAAGCATCCAGCCCAACGATTTCAAGAAGATGAAGGAGTTCCTTGCCTCTGTAGT ACAGGACTTTGACGTGTCTCTGAACAGAGTCAGGATTGGAGCTGCCCAGTTCTCCGACACCTATCATCCT GAGTTTCCACTGGGGACATTCATCGGTGAAAAGGAGATCTCTTTCCAGATTGAGAACATCAAGCAGATCT TTGGCAACACACATATCGGAGCTGCATTGCGCGAGGTGGAGCACTACTTCAGGCCCGACATGGGTTCCCG AATAAACACTGGGACTCCACAGGTCCTCCTTGTCCTGACTGATGGGCAGTCCCAAGACGAGGTGGCCCAG GCAGCGGAAGCTCTTCGACACCGGGGTATAGACATTTACTCTGTGGGTATTGGCGATGTCGACGACCAAC AGCTGATCCAGATCACAGGGACCGCGGAGAAAAAGTTGACCGTTCATAACTTCGATGAACTGAAGAAGGT GAATAAGCGGATAGTTAGAAACATATGTACCACCGCGGGCGAAAGTAATTGCTTCGTGGACGTCGTCGTT GGGTTCGACGTATCCACTCAGGAAAAGGGGCAGACACTTCTTGAGGGACAACCTTGGATGGAAACCTACC TCCAGGACATTCTGAGGGCTATTAGTTCACTGAATGGCGTCTCTTGCGAGGTAGGAACCGAGACGCAAGT CAGTGTGGCTTTTCAGGTGACAAATGCTATGGAAAAGTATAGCCCGAAATTTGAGATTTACAGCGAGAAC ATTCTCAATAGCCTGAAGGATATCACAGTTAAGGGGCCCTCACTCTTGAATGCTAACCTGCTGGATTCCT TGTGGGATACCTTTCAGAATAAGTCCGCCGCTAGGGGAAAGGTAGTGCTCCTGTTCTCAGACGGCCTGGA CGACGACGTTGAGAAACTTGAGCAGAAAAGTGACGAATTGCGGAAAGAGGGTCTGAACGCACTGATCACC GTGGCCCTTGATGGACCTGCCGATAGCTCCGATCTGGCCGATCTCCCCTACATAGAGTTCGGAAAGGGTT TCGAGTACCGCACCCAACTGTCTATTGGCATGAGAGAACTCGGAAGTCGGTTGAGCAAGCAGCTGGTCAA CGTTGCCGAAAGAACTTGCTGTTGTCTGTTTTGTAAGTGTATCGGCGGAGATGGAACTATGGGGGACCCT GGGCCACCTGGCAAGAGGGGACCCCCCGGCTTTAAGGGTAGCGAGGGCTACCTGGGTGAGGAGGGCATTG CGGGAGAACGAGGCGCACCCGGCCCTGTCGGCGAGCAGGGGACCAAAGGGTGCTACGGCACCAAAGGGCC CAAGGGAAATAGGGGACTCAATGGACAAGAGGGGGAAGTGGGGGAAAACGGAATCGATGGACTGAACGGC GAACAGGGCGACAACGGTCTGCCCGGGCGCAAGGGCGAGAAGGGCGATGAGGGGAGTCAGGGGAGTCCTG GGAAAAGAGGCACTCCAGGAGATAGAGGGGCCAAAGGCCTGCGAGGCGATCCTGGCGCTCCTGGCGTGGA TTCCTCTATCGAAGGACCAACAGGCCTGAAAGGAGAGCGGGGCAGACAGGGACGGAGAGGATGGCCAGGC CCCCCAGGCACCCCGGGCTCACGCAGAAAGACCGCAGCACACGGAAGAAGAGGGCACACAGGTCCACAGG GCACGGCCGGCATCCCTGGTCCTGATGGGCTGGAAGGGAGTCTGGGATTGAAAGGACCTCAGGGTCCTCG CGGGGAGGCAGGCGTCAAAGGCGAAAAAGGCGGAGTGGGTAGCAAAGGGCCCCAAGGACCCCCCGGTCCC GGCGGCGAAGCAGGAAACCAGGGGAGGCTCGGCTCTCAGGGCAACAAGGGTGAACCCGGTGATCTGGGGG AAAAGGGTGCCGTCGGATTTCCTGGGCCACGGGGTCTGCAGGGGAACGATGGTAGCCCTGGATATGGCAG TGTCGGCAGGAAAGGTGCCAAGGGTCAGGAGGGCTTCCCCGGAGAAAGTGGTCCCAAGGGTGAGATTGGA GATCCAGGGGGACCCGGTGAAACTGGCTTGAAAGGCGCCCGGGGTAAAATGATCTCTGCTGGATTGCCAG GTGAAATGGGCTCCCCTGGCGAGCCCGGTCCACCCGGAAGGAAAGGCGTGAAGGGAGCAAAAGGACTCGC GTCCTTCAGCACCTGTGAGCTGATTCAGTATGTGAGGGACCGAAGCCCTGGACGCCATGGAAAGCCCGAG TGCCCGGTCCATCCCACGGAACTGGTTTTCGCACTTGACCATAGTAGGGATGTTACAGAACAGGAGTTTG AACGCATGAAGGAGATGATGGCTTTTCTGGTCAGAGACATTAAGGTCCGGGAGAATAGCTGTCCCGTGGG AGCCCATATCGCTATCCTGAGCTACAATTCTCATGCTAGACACTTGGTTCGATTTTCAGATGCCTATAAA AAGAGTCAGCTGCTGAGAGAGATCGAGACAATCCCGTATGAGAGATCATCCGCGTCTCGCGAGATCGGGA GGGCCATGCGATTCATCTCTAGGAATGTCTTTAAAAGGACTCTCCCAGGTGCTCACACACGCAAAATAGC AACATTCTTTAGCAGCGGACAGAGCGCCGATGCCCACAGTATCACTACTGCCGCCATGGAGTTCGGCGCC CTGGAGATAATCCCGGTGGTCATAACTTTTAGCAATGTCCCTAGTGTGAGGCGGGCCTTCGCTATTGATG ATACAGGCACGTTTCAGGTGATTGTGGTGCCTAGCGGAGCCGATTACATTCCTGCACTGGAGCGGCTGCA GCGATGTACCTTTTGCTACGACGTATGTAAACCCGATGCATCTTGCGATCAGGCACGGCCCCCTCCCGTA CAAAGCTACATGGATGCGGCCTTCCTGCTTGACGCCAGTAGGAATATGGGAAGCGCCGAATTTGAGGACA TTCGCGCCTTCCTGGGCGCTCTGCTGGATCACTTCGAAATTACTCCAGAACCAGAGACATCAGTCACCGG GGACCGGGTCGCTCTTTTGAGTCACGCCCCACCCGATTTCCTGCCGAACACTCAAAAGAGCCCCGTGAGA GCCGAATTTAACCTTACCACGTATAGATCTAAAAGGTTGATGAAGAGGCACGTGCACGAGTCCGTCAAAC AGCTCAACGGGGACGCATTCATCGGTCACGCATTGCAATGGACTCTCGACAATGTCTTTCTGAGCACTCC TAATTTGCGAAGGAACAAGGTGATTTTCGTTATCAGTGCAGGGGAAACGAGCCACCTGGATGGTGAAATT CTTAAAAAGGAGTCTCTGCGCGCCAAGTGCCAGGGGTACGCACTGTTTGTCTTTTCTCTTGGCCCTATTT GGGATGACAAAGAGCTGGAGGATTTGGCCTCACATCCGCTTGACCACCATCTCGTGCAGCTGGGTCGGAT TCACAAACCCGATCACTCATACGGCGTAAAGTTTGTAAAGAGTTTCATTAATAGCATAAGACGAGCCATT AACAAATACCCTCCTATAAATCTCAAAATTAAATGCAATCGACTGAACAGCATAGACCCTAAGCAGCCTC CGAGGCCGTTTCGAAGCTTCGTGCCAGGCCCACTGAAAGCCACCCTGAAAGAGGATGTACTTCAGAAAGC GAAGTTCTTCCAAGATAAAAAGTACCTCTCCCGCGTTGCCAGGAGCGGCAGGGACGACGCCATTCAGAAC TTTATGCGGTCTACCTCACACACCTTCAAGAATGGCAGAATGATTGAGTCCGCTCCCAAACAGCACGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222036 representing NM_001102608
Red=Cloning site Green=Tags(s) MMLLILFLVIICSHISVNQDSGPEYADVVFLVDSSDRLGSKSFPFVKMFITKMISSLPIEADKYRVALAQ YSDKLHSEFHLSTFKGRSPMLNHLRKNFGFIGGSLQIGKALQEAHRTYFSAPANGRDKKQFPPILVVLAS SESEDNVEEASKALRKDGVKIISVGVQKASEENLKAMATSQFHFNLRTVRDLSMFSQNMTHIIKDVIKYK EGAVDDIFVEACQGPSMADVVFLLDMSINGSEENFDYLKGFLEESVSALDIKENCMRVGLVAYSNETKVI NSLSMGINKSEVLQHIQNLSPRTGKAYTGAAIKKLRKEVFSARNGSRKNQGVPQIAVLVTHRDSEDNVTK AAVNLRREGVTIFTLGIEGASDTQLEKIASHPAEQYVSKLKTFADLAAHNQTFLKKLRNQITHTVSVFSE RTETLKSGCVDTEEADIYLLIDGSGSTQATDFHEMKTFLSEVVGMFNIAPHKVRVGAVQYADSWDLEFEI NKYSNKQDLGKAIENIRQMGGNTNTGAALNFTLSLLQKAKKQRGNKVPCHLVVLTNGMSKDSILEPANRL REEHIRVYAIGIKEANQTQLREIAGEEKRVYYVHDFDALKDIRNQVVQEICTEEACKEMKADIMFLVDSS GSIGPENFSKMKTFMKNLVSKSQIGPDRVQIGVVQFSDINKEEFQLNRFMSQSDISNAIDQMAHIGQTTL TGSALSFVSQYFSPTKGARPNIRKFLILITDGEAQDIVKEPAVVLRQEGVIIYSVGVFGSNVTQLEEISG RPEMVFYVENFDILQRIEDDLVFGICSPREECKRIEVLDVVFVIDSSGSIDYDEYNIMKDFMIGLVKKAD VGKNQVRFGALKYADDPEVLFYLDDFGTKLEVISVLQNDQAMGGSTYTAEALGFSDHMFTEARGSRLNKG VPQVLIVITDGESHDADKLNATAKALRDKGILVLAVGIDGANPVELLAMAGSSDKYFFVETFGGLKGIFS DVTASVCNSSKVDCEIDKVDLVFLMDGSTSIQPNDFKKMKEFLASVVQDFDVSLNRVRIGAAQFSDTYHP EFPLGTFIGEKEISFQIENIKQIFGNTHIGAALREVEHYFRPDMGSRINTGTPQVLLVLTDGQSQDEVAQ AAEALRHRGIDIYSVGIGDVDDQQLIQITGTAEKKLTVHNFDELKKVNKRIVRNICTTAGESNCFVDVVV GFDVSTQEKGQTLLEGQPWMETYLQDILRAISSLNGVSCEVGTETQVSVAFQVTNAMEKYSPKFEIYSEN ILNSLKDITVKGPSLLNANLLDSLWDTFQNKSAARGKVVLLFSDGLDDDVEKLEQKSDELRKEGLNALIT VALDGPADSSDLADLPYIEFGKGFEYRTQLSIGMRELGSRLSKQLVNVAERTCCCLFCKCIGGDGTMGDP GPPGKRGPPGFKGSEGYLGEEGIAGERGAPGPVGEQGTKGCYGTKGPKGNRGLNGQEGEVGENGIDGLNG EQGDNGLPGRKGEKGDEGSQGSPGKRGTPGDRGAKGLRGDPGAPGVDSSIEGPTGLKGERGRQGRRGWPG PPGTPGSRRKTAAHGRRGHTGPQGTAGIPGPDGLEGSLGLKGPQGPRGEAGVKGEKGGVGSKGPQGPPGP GGEAGNQGRLGSQGNKGEPGDLGEKGAVGFPGPRGLQGNDGSPGYGSVGRKGAKGQEGFPGESGPKGEIG DPGGPGETGLKGARGKMISAGLPGEMGSPGEPGPPGRKGVKGAKGLASFSTCELIQYVRDRSPGRHGKPE CPVHPTELVFALDHSRDVTEQEFERMKEMMAFLVRDIKVRENSCPVGAHIAILSYNSHARHLVRFSDAYK KSQLLREIETIPYERSSASREIGRAMRFISRNVFKRTLPGAHTRKIATFFSSGQSADAHSITTAAMEFGA LEIIPVVITFSNVPSVRRAFAIDDTGTFQVIVVPSGADYIPALERLQRCTFCYDVCKPDASCDQARPPPV QSYMDAAFLLDASRNMGSAEFEDIRAFLGALLDHFEITPEPETSVTGDRVALLSHAPPDFLPNTQKSPVR AEFNLTTYRSKRLMKRHVHESVKQLNGDAFIGHALQWTLDNVFLSTPNLRRNKVIFVISAGETSHLDGEI LKKESLRAKCQGYALFVFSLGPIWDDKELEDLASHPLDHHLVQLGRIHKPDHSYGVKFVKSFINSIRRAI NKYPPINLKIKCNRLNSIDPKQPPRPFRSFVPGPLKATLKEDVLQKAKFFQDKKYLSRVARSGRDDAIQN FMRSTSHTFKNGRMIESAPKQHD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001102608 |
| ORF Size | 6789 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001102608.1, NP_001096078.1 |
| RefSeq Size | 9572 bp |
| RefSeq ORF | 6792 bp |
| Locus ID | 131873 |
| UniProt ID | A6NMZ7 |
| Cytogenetics | 3q22.1 |
| Protein Pathways | ECM-receptor interaction, Focal adhesion |
| MW | 247.2 kDa |
| Gene Summary | This gene encodes a large protein that contains multiple von Willebrand factor domains and forms a component of the basal lamina of epithelial cells. This protein may regulate epithelial cell-fibronectin interactions. Variation in this gene may be implicated in skin diseases. [provided by RefSeq, May 2017] |
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