PCDHGB6 (NM_032100) Human Tagged ORF Clone

CAT#: RC221985

PCDHGB6 (Myc-DDK-tagged)-Human protocadherin gamma subfamily B, 6 (PCDHGB6), transcript variant 2

Product Images

Circular map for RC221985

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHGB6
• Synonyms: PCDH-GAMMA-B6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221985 representing NM_032100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGGGAGCTGCGCGCAGAGGCGCCGGGCCGGCCCGCGGCAGGTGCTATTTCCTTTGCTGCTGCCTT
  TGTTCTACCCCACCCTGAGTGAGCCGATCCGCTACTCGATTCCGGAGGAGCTGGCCAAGGGCTCGGTGGT
  GGGGAACCTCGCTAAGGATCTAGGGCTCAGTGTCCTGGATGTGTCGGCTCGCAAGCTGCGAGTGAGCGCG
  GAGAAGCTGCACTTCAGCGTAGACGCGGAGAGCGGGGACTTACTTGTGAAGAACCGAATAGACCGTGAGC
  AAATATGCAAAGAGAGAAGAAGATGTGAGTTGCAATTGGAAGCTGTGGTGGAAAATCCTTTAAATATTTT
  TCATGTCATTGTGGTGATTGAGGATGTTAATGACCACGCCCCTCAATTTGATAAAAAGGAAATACATTTA
  GAAATTTTCGAATCTGCATCCGCTGGTACACGACTATCGCTTGACCCTGCCACGGATCCTGATATAAACA
  TAAACTCAATTAAAGATTATAAGATAAACTCTAATCCTTATTTTTCATTAATGGTTAGAGTTAATTCCGA
  TGGTGGCAAATACCCAGAGTTATCTCTGGAGAAACTCCTAGACCGGGAAGAACAGAGATCTCATAGCTTG
  ATATTGACTGCCTTGGACGGAGGGGACCCACCAAGAAGTGCCACCGCTCACATAGAAATTTCTGTCAAGG
  ATACCAATGATAACCCCCCGGTTTTCAGCAGAGACGAATATAGAATTAGTCTTAGTGAAAATCTGCCCCC
  TGGGTCCCCTGTGTTGCAAGTGACAGCCACTGACCAGGATGAGGGGGTCAATGCTGAGATAAACTACTAC
  TTCCGAAGCACTGCCCAGAGCACAAAACATATGTTCTCATTGGATGAGAAAACAGGTATGATTAAGAATA
  ACCAGTCATTTGATTTTGAAGATGTAGAAAGGTACACCATGGAAGTGGAAGCGAAGGACGGAGGTGGTCT
  CTCTACCCAGTGTAAAGTAATCATAGAAATCCTTGATGAAAACGACAACAGCCCAGAAATAATCATCACT
  TCTCTCTCTGATCAGATTTTGGAGAATTCACCTCCAGGAATGGTTGTTGCCCTCTTCAAAACACGGGATC
  TGGATTTCGGAGGAAATGGAGAAGTCAGGTGTAATATAGAAACAGACATTCCATTCAAGATTTATTCTTC
  TTCCAATAACTACTACAAACTGGTGACAGATGGAGCCCTGGACCGAGAGCAGACACCAGAATACAATGTC
  ACCATCGTAGCCACTGACAGGGGCAAGCCGCCTCTTTCTTCCAGTAGAAGCATCACCTTGTATGTCGCTG
  ACATCAACGACAACGCCCCAGTTTTCGACCAGACGTCCTACGTGGTCCACGTGGCCGAGAACAACCCGCC
  AGGAGCCTCCATTGCGCAAGTGAGCGCCTCTGACCCGGATTTGGGGCTCAATGGCCACATCTCCTACTCT
  ATAGTGGCGAGTGACCTAGAGCCCCTGGCGGTGTCGTCATACGTGTCAGTGAGCGCGCAGAGCGGGGTGG
  TGTTCGCGCAGCGCGCCTTTGATCACGAGCAGCTGCGCGCCTTCGCGCTCACGCTGCAGGCCCGCGACCA
  CGGCTCGCCCACGCTCAGCGCCAACGTGAGCCTGCGCGTGTTGGTGGGAGACCGCAATGACAACGCACCG
  CGGGTGCTGTACCCAGCTCTGGGTCCTGACGGCTCCGCGTTCTTCGATATGGTACCTCGCTCTGCAGAGC
  CCGGCTACCTAGTGACTAAGGTGGTAGCGGTGGACGCCGACTCGGGACACAACGCCTGGCTGTCCTACCA
  CGTGCTGCAGGCCAGTGAGCCCGGACTCTTCAGCCTGGGGCTGCGCACTGGGGAGGTGCGCACGGCTCGA
  GCCTTAGGCGACAGGGACGCAGCCCGCCAGCGCCTGCTGGTCGCTGTGCGTGACGGTGGACAGCCGCCAC
  TCTCTGCCACCGCCACGCTTCATCTGGTCTTCGCAGACAACTTGCAAGAGATACTGCCAGACCTCAGCGA
  CCGCCCTGTACTCTCTGACCCCCAGGCTGAACTGCAGTTTTACCTGGTGGTGGCCTTGGCCTTAATCTCA
  GTGCTCTTCCTCCTCGCCGTGATTCTGGCCATTGCCTTGCGCCTGCGACGCTCTCTCAGCCCTGCTACTT
  GGGACTGCTTCCATCCTGGTCTCTGTGTCAAGTCTGGACCTGTAGTTCCCCCCAACTACAGTGAGGGGAC
  TTTGCCTTATTCTTATAATCTGTGCATTGCACATACGGGTACAAAAGAGTTTAATTTCCTAAAATGCAGT
  GTGCCCCTACATTCCAATGAAGACATGGTTTGCAGTGTTTCTCCTGGAGCCTTAATTCCACCTCATGGTG
  GGGAGGATTTGACTTCACATCCTGAGACTCTGACTTCGGTGAGTTTCTCTTTTTTGTGTGTGATTTATCT
  AATAGTCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221985 representing NM_032100
  Red=Cloning site Green=Tags(s)
  
  MGGSCAQRRRAGPRQVLFPLLLPLFYPTLSEPIRYSIPEELAKGSVVGNLAKDLGLSVLDVSARKLRVSA
  EKLHFSVDAESGDLLVKNRIDREQICKERRRCELQLEAVVENPLNIFHVIVVIEDVNDHAPQFDKKEIHL
  EIFESASAGTRLSLDPATDPDININSIKDYKINSNPYFSLMVRVNSDGGKYPELSLEKLLDREEQRSHSL
  ILTALDGGDPPRSATAHIEISVKDTNDNPPVFSRDEYRISLSENLPPGSPVLQVTATDQDEGVNAEINYY
  FRSTAQSTKHMFSLDEKTGMIKNNQSFDFEDVERYTMEVEAKDGGGLSTQCKVIIEILDENDNSPEIIIT
  SLSDQILENSPPGMVVALFKTRDLDFGGNGEVRCNIETDIPFKIYSSSNNYYKLVTDGALDREQTPEYNV
  TIVATDRGKPPLSSSRSITLYVADINDNAPVFDQTSYVVHVAENNPPGASIAQVSASDPDLGLNGHISYS
  IVASDLEPLAVSSYVSVSAQSGVVFAQRAFDHEQLRAFALTLQARDHGSPTLSANVSLRVLVGDRNDNAP
  RVLYPALGPDGSAFFDMVPRSAEPGYLVTKVVAVDADSGHNAWLSYHVLQASEPGLFSLGLRTGEVRTAR
  ALGDRDAARQRLLVAVRDGGQPPLSATATLHLVFADNLQEILPDLSDRPVLSDPQAELQFYLVVALALIS
  VLFLLAVILAIALRLRRSLSPATWDCFHPGLCVKSGPVVPPNYSEGTLPYSYNLCIAHTGTKEFNFLKCS
  VPLHSNEDMVCSVSPGALIPPHGGEDLTSHPETLTSVSFSFLCVIYLIVY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032100
• ORF Size: 2460 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032100.1, NP_115271.1
• RefSeq Size: 2463 bp
• RefSeq ORF: 2463 bp
• Locus ID: 56100
• UniProt ID: Q9Y5F9
• Cytogenetics: 5q31.3
• MW: 89.8 kDa
• Gene Summary: This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]