ZNF331 (NM_001079906) Human Tagged ORF Clone

CAT#: RC221898

ZNF331 (Myc-DDK-tagged)-Human zinc finger protein 331 (ZNF331), transcript variant 2. Note: ORF is codon optimized

Product Images

Circular map for RC221898

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF331
• Synonyms: RITA; ZNF361; ZNF463
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221898 ORF sequence, codon optimized.
  Due to the complexity of NM_001079906, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCAGGGGTTGGTTACTTTTGCAGACGTCGCCATCGACTTCAGCCAGGAGGAGTGGGCTTGTCTGA
  ACAGCGCTCAAAGGGACCTTTACTGGGACGTGATGCTGGAGAACTACAGTAATCTCGTGAGTCTCGACCT
  CGAGTCCGCATACGAAAATAAGAGCCTGCCAACCGAAAAGAACATACATGAGATTCGAGCATCCAAGCGG
  AATTCCGATCGCAGATCTAAGTCACTGGGGCGGAACTGGATCTGCGAGGGAACACTGGAGAGGCCACAGC
  GCTCTCGGGGACGATACGTCAACCAGATGATCATCAATTATGTTAAACGCCCCGCAACCAGAGAGGGAAC
  ACCTCCACGAACCCACCAGAGACATCATAAAGAAAACTCATTCGAATGCAAAGACTGCGGCAAGGCCTTC
  AGCCGGGGTTACCAACTTTCTCAGCACCAGAAAATTCACACAGGCGAGAAACCCTATGAATGTAAGGAGT
  GTAAAAAAGCATTCCGCTGGGGAAACCAGCTGACACAGCACCAGAAGATACACACCGGTGAGAAACCATA
  CGAATGCAAGGACTGTGGTAAAGCCTTCCGCTGGGGGAGTAGTCTGGTTATCCACAAAAGGATACATACC
  GGAGAGAAGCCTTATGAATGCAAGGATTGCGGAAAAGCCTTCCGGCGCGGAGACGAACTTACCCAACATC
  AGAGATTCCACACAGGTGAGAAAGATTATGAGTGCAAAGACTGTGGCAAAACTTTTTCTCGAGTGTATAA
  ATTGATCCAGCACAAAAGGATACATTCTGGCGAGAAACCCTATGAGTGTAAGGACTGTGGAAAAGCTTTC
  ATTTGTGGCAGCTCACTGATTCAACATAAGCGCATACATACCGGCGAAAAGCCTTACGAGTGCCAAGAGT
  GTGGAAAGGCTTTCACCAGAGTGAATTATCTCACCCAACACCAAAAAATCCATACGGGGGAAAAGCCCCA
  CGAGTGCAAAGAGTGCGGCAAGGCATTCCGGTGGGGCTCTAGCCTGGTCAAACACGAACGAATCCACACC
  GGCGAAAAACCGTACAAATGTACTGAATGCGGCAAGGCCTTCAACTGTGGCTACCACCTTACCCAACACG
  AAAGAATACACACTGGAGAAACTCCGTACAAATGCAAAGAGTGCGGCAAAGCTTTTATTTACGGATCCTC
  ATTGGTTAAACACGAAAGGATTCATACCGGTGTAAAACCTTATGGATGTACTGAGTGCGGGAAGTCCTTT
  TCACACGGGCATCAACTGACACAGCACCAGAAGACTCACAGCGGTGCAAAAAGCTATGAATGTAAAGAAT
  GCGGTAAGGCCTGCAACCACCTGAACCACCTGCGCGAGCACCAGCGGATTCACAACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221898 representing NM_001079906
  Red=Cloning site Green=Tags(s)
  
  MAQGLVTFADVAIDFSQEEWACLNSAQRDLYWDVMLENYSNLVSLDLESAYENKSLPTEKNIHEIRASKR
  NSDRRSKSLGRNWICEGTLERPQRSRGRYVNQMIINYVKRPATREGTPPRTHQRHHKENSFECKDCGKAF
  SRGYQLSQHQKIHTGEKPYECKECKKAFRWGNQLTQHQKIHTGEKPYECKDCGKAFRWGSSLVIHKRIHT
  GEKPYECKDCGKAFRRGDELTQHQRFHTGEKDYECKDCGKTFSRVYKLIQHKRIHSGEKPYECKDCGKAF
  ICGSSLIQHKRIHTGEKPYECQECGKAFTRVNYLTQHQKIHTGEKPHECKECGKAFRWGSSLVKHERIHT
  GEKPYKCTECGKAFNCGYHLTQHERIHTGETPYKCKECGKAFIYGSSLVKHERIHTGVKPYGCTECGKSF
  SHGHQLTQHQKTHSGAKSYECKECGKACNHLNHLREHQRIHNS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079906
• ORF Size: 1389 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079906.1, NP_001073375.1
• RefSeq Size: 4151 bp
• RefSeq ORF: 1392 bp
• Locus ID: 55422
• UniProt ID: Q9NQX6
• Cytogenetics: 19q13.42
• Protein Families: Transcription Factors
• MW: 53.7 kDa
• Gene Summary: This gene encodes a zinc finger protein containing a KRAB (Kruppel-associated box) domain found in transcriptional repressors. This gene may be methylated and silenced in cancer cells. This gene is located within a differentially methylated region (DMR) and shows allele-specific expression in placenta. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding the same protein. [provided by RefSeq, Nov 2015]