PHF19 (NM_015651) Human Tagged ORF Clone

CAT#: RC221894

PHF19 (Myc-DDK-tagged)-Human PHD finger protein 19 (PHF19), transcript variant 1

Product Images

Circular map for RC221894

• 

  Western blot validation of overexpression lysate (Cat# LY414412) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221894 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHF19
• Synonyms: MTF2L1; PCL3; TDRD19B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221894 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAATCGAGCTCTGGATCCAGGGACTCGGGACTCCTATGGTGCCACCAGCCACCTCCCCAACAAGG
  GGGCCCTGGCGAAGGTCAAGAACAACTTCAAAGACTTGATGTCCAAACTGACGGAGGGCCAGTATGTGCT
  GTGCCGGTGGACAGATGGCCTGTACTACCTCGGGAAGATCAAGAGGGTCAGCAGCTCTAAGCAAAGCTGC
  CTCGTGACTTTCGAAGATAATTCCAAATACTGGGTCCTATGGAAGGACATACAGCATGCCGGTGTTCCAG
  GAGAGGAGCCCAAGTGCAACATCTGCCTAGGGAAGACATCAGGGCCGCTGAATGAGATCCTCATCTGCGG
  GAAGTGTGGCCTGGGTTACCACCAGCAGTGCCACATCCCCATAGCGGGCAGTGCTGACCAGCCCCTGCTC
  ACACCTTGGTTCTGCCGACGCTGCATCTTCGCACTGGCTGTGCGGAAAGGCGGCGCGCTGAAGAAGGGCG
  CCATCGCCAGGACGCTGCAGGCCGTGAAGATGGTGCTGTCCTACCAGCCCGAGGAGCTCGAGTGGGACTC
  GCCCCATCGCACCAACCAGCAGCAATGCTACTGCTACTGCGGCGGGCCCGGAGAATGGTACCTGCGGATG
  CTGCAATGTTACCGGTGCAGGCAGTGGTTCCACGAGGCCTGCACCCAGTGCCTCAATGAGCCCATGATGT
  TTGGAGACCGGTTTTACCTGTTCTTCTGCTCCGTGTGTAACCAGGGCCCAGAGTACATCGAGAGGCTGCC
  CCTGCGATGGGTGGATGTGGTTCACCTGGCCCTCTATAATCTGGGGGTACAGAGCAAGAAGAAGTACTTT
  GACTTTGAGGAGATTCTGGCCTTTGTCAACCACCACTGGGAGCTCCTGCAGCTTGGCAAGCTCACCAGCA
  CCCCAGTGACAGATCGAGGACCACATCTCCTCAACGCTCTGAACAGTTATAAAAGCCGGTTCCTCTGCGG
  CAAGGAGATCAAGAAGAAGAAGTGCATCTTCCGCCTGCGCATCCGCGTCCCACCCAACCCGCCAGGGAAG
  CTGCTGCCTGACAAAGGACTGCTGCCAAATGAGAACAGCGCCTCCTCTGAGCTGCGTAAGAGAGGAAAGA
  GCAAGCCTGGTTTGTTGCCTCACGAATTCCAGCAGCAGAAAAGGCGAGTTTATAGAAGAAAAAGATCAAA
  GTTTTTGCTGGAAGATGCTATTCCCAGTAGTGACTTCACCTCAGCCTGGAGCACCAACCACCACCTGGCT
  AGCATATTTGACTTCACGCTGGATGAAATTCAAAGTTTAAAAAGTGCCAGCTCAGGCCAGACCTTCTTCT
  CAGATGTCGACTCCACCGACGCTGCCAGCACCTCTGGCTCTGCCTCCACCAGCCTCTCCTATGACTCCAG
  ATGGACAGTGGGCAGCCGAAAGAGGAAGCTGGCAGCCAAGGCATACATGCCCCTGCGGGCAAAGCGGTGG
  GCAGCTGAGCTGGATGGACGCTGCCCCTCGGACAGCAGTGCAGAGGGGGCTTCAGTCCCCGAGCGGCCAG
  ACGAAGGCATTGACAGCCACACATTTGAGAGCATCAGTGAAGATGACTCATCCCTGTCCCACCTCAAGTC
  ATCTATCACCAACTACTTTGGTGCAGCTGGGCGGTTGGCCTGTGGGGAGAAGTACCAGGTGTTGGCTCGG
  AGGGTCACACCTGAGGGCAAGGTTCAGTACCTGGTGGAGTGGGAAGGGACCACCCCTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221894 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MENRALDPGTRDSYGATSHLPNKGALAKVKNNFKDLMSKLTEGQYVLCRWTDGLYYLGKIKRVSSSKQSC
  LVTFEDNSKYWVLWKDIQHAGVPGEEPKCNICLGKTSGPLNEILICGKCGLGYHQQCHIPIAGSADQPLL
  TPWFCRRCIFALAVRKGGALKKGAIARTLQAVKMVLSYQPEELEWDSPHRTNQQQCYCYCGGPGEWYLRM
  LQCYRCRQWFHEACTQCLNEPMMFGDRFYLFFCSVCNQGPEYIERLPLRWVDVVHLALYNLGVQSKKKYF
  DFEEILAFVNHHWELLQLGKLTSTPVTDRGPHLLNALNSYKSRFLCGKEIKKKKCIFRLRIRVPPNPPGK
  LLPDKGLLPNENSASSELRKRGKSKPGLLPHEFQQQKRRVYRRKRSKFLLEDAIPSSDFTSAWSTNHHLA
  SIFDFTLDEIQSLKSASSGQTFFSDVDSTDAASTSGSASTSLSYDSRWTVGSRKRKLAAKAYMPLRAKRW
  AAELDGRCPSDSSAEGASVPERPDEGIDSHTFESISEDDSSLSHLKSSITNYFGAAGRLACGEKYQVLAR
  RVTPEGKVQYLVEWEGTTPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015651
• ORF Size: 1740 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015651.3
• RefSeq Size: 4310 bp
• RefSeq ORF: 1743 bp
• Locus ID: 26147
• UniProt ID: Q5T6S3
• Cytogenetics: 9q33.2
• Protein Families: Druggable Genome
• MW: 65.6 kDa
• Gene Summary: Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.[UniProtKB/Swiss-Prot Function]