Complement C8A (C8A) (NM_000562) Human Tagged ORF Clone

CAT#: RC221824

C8A (Myc-DDK-tagged)-Human complement component 8, alpha polypeptide (C8A)

Product Images

Circular map for RC221824

• 

  Western blot validation of overexpression lysate (Cat# LY424641) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221824 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Complement C8A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221824 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGCTGTTGTTTTCTTCATCTTGTCTTTGATGACTTGTCAGCCTGGGGTAACTGCACAGGAGAAGG
  TGAACCAGAGAGTAAGACGGGCAGCTACACCCGCAGCAGTTACCTGCCAGCTGAGCAACTGGTCAGAGTG
  GACAGATTGCTTTCCGTGCCAGGACAAAAAGTACCGACACCGGAGCCTCTTGCAGCCAAACAAGTTTGGG
  GGAACCATCTGCAGTGGTGACATCTGGGATCAAGCCAGCTGCTCCAGTTCTACAACTTGTGTAAGGAAAG
  CACAGTGTGGACAGGATTTCCAGTGTAAGGAGACAGGTCGCTGCCTGAAACGCCACCTTGTGTGTAATGG
  AGACCAGGACTGCCTTGATGGCTCTGATGAGGACGACTGTGAAGATGTCAGGGCCATTGACGAAGACTGC
  AGCCAGTATGAACCAATTCCAGGATCACAGAAGGCAGCCTTGGGGTACAATATCCTGACCCAGGAAGATG
  CTCAGAGTGTGTACGATGCCAGTTATTATGGGGGCCAGTGTGAGACGGTATACAATGGGGAATGGAGGGA
  GCTTCGATATGACTCCACCTGTGAACGTCTCTACTATGGAGATGATGAGAAATACTTTCGGAAACCCTAC
  AACTTTCTGAAGTACCACTTTGAAGCCCTGGCAGATACTGGAATCTCCTCAGAGTTTTATGATAATGCAA
  ATGACCTTCTTTCCAAAGTTAAAAAAGACAAGTCTGACTCATTTGGAGTGACCATCGGCATAGGCCCAGC
  CGGCAGCCCTTTATTGGTGGGTGTAGGTGTATCCCACTCACAAGACACTTCATTCTTGAACGAATTAAAC
  AAGTATAATGAGAAGAAATTCATTTTCACAAGAATCTTCACAAAGGTGCAGACTGCACATTTTAAGATGA
  GGAAGGATGACATTATGCTGGATGAAGGAATGCTGCAGTCATTAATGGAGCTTCCAGATCAGTACAATTA
  TGGCATGTATGCCAAGTTCATCAATGACTATGGCACCCATTACATCACATCTGGATCCATGGGTGGCATT
  TATGAATATATCCTGGTGATTGACAAAGCAAAAATGGAATCCCTTGGTATTACCAGCAGAGATATCACGA
  CATGTTTTGGAGGCTCCTTGGGCATTCAATATGAAGACAAAATAAATGTTGGTGGAGGTTTATCAGGAGA
  CCATTGTAAAAAATTTGGAGGTGGCAAAACTGAAAGGGCCAGGAAGGCCATGGCTGTGGAAGACATTATT
  TCTCGGGTGCGAGGTGGCAGTTCTGGCTGGAGCGGTGGCTTGGCACAGAACAGGAGCACCATTACATACC
  GTTCCTGGGGGAGGTCATTAAAGTATAATCCTGTTGTTATCGATTTTGAGATGCAGCCTATCCACGAGGT
  GCTGCGGCACACAAGCCTGGGGCCTCTGGAGGCCAAGCGCCAGAACCTGCGCCGCGCCTTGGACCAGTAT
  CTGATGGAATTCAATGCCTGCCGATGTGGGCCTTGCTTCAACAATGGGGTGCCCATCCTCGAGGGCACCA
  GCTGCAGGTGCCAGTGCCGCCTGGGTAGCTTGGGTGCTGCCTGTGAGCAAACACAGACAGAAGGAGCCAA
  AGCAGATGGGAGCTGGAGTTGCTGGAGCTCCTGGTCTGTATGCAGAGCAGGCATCCAGGAAAGGAGAAGA
  GAGTGTGACAATCCAGCACCTCAGAATGGAGGGGCCTCGTGTCCAGGGCGGAAAGTACAGACGCAGGCTT
  GC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221824 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFAVVFFILSLMTCQPGVTAQEKVNQRVRRAATPAAVTCQLSNWSEWTDCFPCQDKKYRHRSLLQPNKFG
  GTICSGDIWDQASCSSSTTCVRKAQCGQDFQCKETGRCLKRHLVCNGDQDCLDGSDEDDCEDVRAIDEDC
  SQYEPIPGSQKAALGYNILTQEDAQSVYDASYYGGQCETVYNGEWRELRYDSTCERLYYGDDEKYFRKPY
  NFLKYHFEALADTGISSEFYDNANDLLSKVKKDKSDSFGVTIGIGPAGSPLLVGVGVSHSQDTSFLNELN
  KYNEKKFIFTRIFTKVQTAHFKMRKDDIMLDEGMLQSLMELPDQYNYGMYAKFINDYGTHYITSGSMGGI
  YEYILVIDKAKMESLGITSRDITTCFGGSLGIQYEDKINVGGGLSGDHCKKFGGGKTERARKAMAVEDII
  SRVRGGSSGWSGGLAQNRSTITYRSWGRSLKYNPVVIDFEMQPIHEVLRHTSLGPLEAKRQNLRRALDQY
  LMEFNACRCGPCFNNGVPILEGTSCRCQCRLGSLGAACEQTQTEGAKADGSWSCWSSWSVCRAGIQERRR
  ECDNPAPQNGGASCPGRKVQTQAC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000562
• ORF Size: 1752 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000562.3
• RefSeq Size: 2392 bp
• RefSeq ORF: 1755 bp
• Locus ID: 731
• UniProt ID: P07357
• Cytogenetics: 1p32.2
• Domains: tsp_1, MACPF, ldl_recept_a
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• MW: 65.2 kDa
• Gene Summary: C8 is a component of the complement system and contains three polypeptides, alpha, beta and gamma. This gene encodes the alpha subunit of C8. C8 participates in the formation of the membrane attack complex (MAC). The MAC assembles on bacterial membranes to form a pore, permitting disruption of bacterial membrane organization. Mutations in this gene cause complement C8 alpha-gamma deficiency. [provided by RefSeq, Nov 2008]