FTSJD1 (CMTR2) (NM_001099642) Human Tagged ORF Clone

CAT#: RC221751

FTSJD1 (Myc-DDK-tagged)-Human FtsJ methyltransferase domain containing 1 (FTSJD1), transcript variant 2

Product Images

Circular map for RC221751

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FTSJD1
• Synonyms: AFT; FTSJD1; HMTr2; MTr2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221751 representing NM_001099642
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAAGTGCAGAAAGACACCAGTTCAGCAGCTAGCAAGTCCCGCGTCATTCAGCCCAGATATTCTTG
  CTGACATTTTTGAACTCTTTGCCAAGAACTTTTCTTATGGCAAGCCACTTAATAATGAGTGGCAGTTACC
  AGATCCCAGTGAGATTTTCACCTGTGACCACACTGAACTTAATGCATTTCTTGATTTGAAGAACTCCCTA
  AATGAAGTAAAAAACCTACTGAGTGATAAGAAACTGGATGAGTGGCATGAGCACACTGCTTTCACTAATA
  AAGCGGGGAAAATCATTTCTCATGTTAGAAAATCTGTGAATGCTGAACTTTGTACTCAAGCATGGTGTAA
  GTTCCATGAGATTTTGTGCAGCTTTCCACTTATTCCACAGGAAGCTTTTCAGAATGGAAAACTGAATTCT
  CTACACCTTTGTGAAGCTCCAGGAGCTTTTATAGCTAGTCTCAACCACTACTTAAAATCCCATCGGTTTC
  CTTGTCATTGGAGTTGGGTAGCGAATACTCTGAATCCATACCATGAAGCAAATGACGACCTCATGATGAT
  TATGGATGACCGGCTTATTGCAAATACCTTGCACTGGTGGTACTTTGGTCCAGATAACACTGGTGATATC
  ATGACCCTGAAATTCTTGACTGGACTTCAGAATTTCATAAGCAGCATGGCTACTGTTCACTTGGTCACTG
  CAGATGGGAGTTTTGATTGCCAAGGAAACCCAGGTGAACAAGAAGCTTTAGTTTCTTCTTTGCATTACTG
  TGAAGTTGTCACTGCTCTGACCACTCTTGGAAACGGTGGCTCTTTTGTTCTAAAGATGTTTACTATGTTT
  GAACATTGTTCCATAAACTTGATGTACCTGCTAAACTGTTGTTTTGACCAAGTCCATGTTTTCAAACCTG
  CTACTAGCAAGGCAGGAAACTCCGAAGTCTATGTGGTTTGCCTCCACTATAAGGGGAGAGAGGCCATCCA
  TCCTCTGTTATCTAAGATGACCTTGAATTTTGGGACTGAAATGAAAAGGAAAGCCCTTTTTCCCCATCAT
  GTGATTCCTGATTCTTTTCTTAAGAGACATGAAGAATGTTGTGTGTTCTTTCATAAATATCAGCTAGAGA
  CTATTTCTGAAAACATTCGTCTATTTGAGTGCATGGGAAAGGCGGAACAAGAAAAGCTGAATAATTTAAG
  GGATTGTGCTATACAATATTTTATGCAAAAATTTCAACTGAAACATCTTTCCAGAAATAATTGGCTAGTA
  AAAAAATCTAGTATTGGTTGTAGTACAAATACAAAATGGTTTGGGCAGAGGAACAAATATTTTAAAACTT
  ATAATGAAAGGAAGATGCTAGAAGCCCTTTCATGGAAAGATAAAGTAGCCAAAGGATACTTTAATAGTTG
  GGCTGAAGAACATGGTGTATATCATCCTGGGCAGAGTTCTATTTTAGAAGGAACAGCTTCCAATCTTGAG
  TGTCACTTATGGCATATTTTGGAGGGAAAGAAACTGCCAAAGGTAAAATGTTCTCCTTTTTGCAATGGTG
  AAATTTTAAAAACTCTTAATGAAGCAATTGAAAAGTCATTAGGAGGAGCTTTTAATTTGGATTCCAAGTT
  TAGGCCAAAACAGCAGTATTCTTGTTCTTGTCATGTTTTTTCTGAAGAACTGATATTTTCCGAGTTGTGT
  AGCCTTACTGAGTGCCTTCAGGATGAGCAGGTTGTAGTACCCAGCAATCAAATAAAGTGCCTGCTGGTGG
  GCTTTTCGACTCTCCGTAATATCAAAATGCATATACCGTTGGAAGTTCGACTCCTAGAATCAGCTGAACT
  CACAACTTTTAGCTGTTCATTGCTTCATGATGGAGATCCAACTTACCAGCGTTTATTTTTGGACTGCCTT
  CTACATTCATTGCGGGAGCTTCATACAGGAGATGTTATGATTTTGCCTGTACTTTCTTGCTTCACAAGAT
  TTATGGCTGGTTTGATCTTTGTACTCCACAGTTGTTTTAGATTCATCACTTTTGTTTGTCCCACATCCTC
  TGATCCCCTGAGGACCTGCGCAGTCCTGCTATGTGTTGGTTATCAGGACCTTCCAAATCCAGTTTTCCGA
  TATTTGCAGAGTGTGAATGAATTGTTGAGCACTTTGCTCAACTCTGACTCACCCCAGCAGGTTTTACAGT
  TTGTGCCAATGGAGGTACTCCTTAAGGGGGCCCTGCTTGATTTTTTGTGGGATTTGAATGCTGCCATTGC
  TAAAAGGCATTTGCATTTCATTATTCAAAGAGAGAGAGAAGAAATTATCAACAGCCTTCAGTTACAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221751 representing NM_001099642
  Red=Cloning site Green=Tags(s)
  
  MSKCRKTPVQQLASPASFSPDILADIFELFAKNFSYGKPLNNEWQLPDPSEIFTCDHTELNAFLDLKNSL
  NEVKNLLSDKKLDEWHEHTAFTNKAGKIISHVRKSVNAELCTQAWCKFHEILCSFPLIPQEAFQNGKLNS
  LHLCEAPGAFIASLNHYLKSHRFPCHWSWVANTLNPYHEANDDLMMIMDDRLIANTLHWWYFGPDNTGDI
  MTLKFLTGLQNFISSMATVHLVTADGSFDCQGNPGEQEALVSSLHYCEVVTALTTLGNGGSFVLKMFTMF
  EHCSINLMYLLNCCFDQVHVFKPATSKAGNSEVYVVCLHYKGREAIHPLLSKMTLNFGTEMKRKALFPHH
  VIPDSFLKRHEECCVFFHKYQLETISENIRLFECMGKAEQEKLNNLRDCAIQYFMQKFQLKHLSRNNWLV
  KKSSIGCSTNTKWFGQRNKYFKTYNERKMLEALSWKDKVAKGYFNSWAEEHGVYHPGQSSILEGTASNLE
  CHLWHILEGKKLPKVKCSPFCNGEILKTLNEAIEKSLGGAFNLDSKFRPKQQYSCSCHVFSEELIFSELC
  SLTECLQDEQVVVPSNQIKCLLVGFSTLRNIKMHIPLEVRLLESAELTTFSCSLLHDGDPTYQRLFLDCL
  LHSLRELHTGDVMILPVLSCFTRFMAGLIFVLHSCFRFITFVCPTSSDPLRTCAVLLCVGYQDLPNPVFR
  YLQSVNELLSTLLNSDSPQQVLQFVPMEVLLKGALLDFLWDLNAAIAKRHLHFIIQREREEIINSLQLQN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099642
• ORF Size: 2310 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099642.2
• RefSeq Size: 4040 bp
• RefSeq ORF: 2313 bp
• Locus ID: 55783
• UniProt ID: Q8IYT2
• Cytogenetics: 16q22.2
• MW: 88.1 kDa
• Gene Summary: S-adenosyl-L-methionine-dependent methyltransferase that mediates mRNA cap2 2'-O-ribose methylation to the 5'-cap structure of mRNAs. Methylates the ribose of the second nucleotide of a m(7)GpppG-capped mRNA and small nuclear RNA (snRNA) (cap0) to produce m(7)GpppRmpNm (cap2). Recognizes a guanosine cap on RNA independently of its N(7) methylation status. Display cap2 methylation on both cap0 and cap1. Displays a preference for cap1 RNAs.[UniProtKB/Swiss-Prot Function]