C14orf68 (SLC25A47) (NM_207117) Human Tagged ORF Clone

CAT#: RC221734

SLC25A47 (Myc-DDK-tagged)-Human solute carrier family 25, member 47 (SLC25A47)

Product Images

Circular map for RC221734

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C14orf68
• Synonyms: C14orf68; HDCMP; HDMCP; HMFN1655
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221734 representing NM_207117
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTTGTCGCTGGAGCCATCGGAGGCGTCTGCGGTGTTGCTGTGGGCTACCCCCTGGACACGGTGA
  AGGTCAGGATCCAGACGGAGCCAAAGTACACAGGCATCTGGCACTGCGTCCGGGATACGTATCACCGAGA
  GCGCGTGTGGGGCTTCTACCGGGGCCTCTCGCTGCCCGTGTGCACGGTGTCCCTGGTATCTTCCGTGTCT
  TTTGGCACCTACCGCCACTGCCTGGCGCACATCTGCCGGCTCCGGTACGGCAACCCTGACGCCAAGCCCA
  CCAAGGCCGACATCACGCTCTCGGGATGCGCCTCCGGCCTCGTCCGCGTGTTCCTGACGTCGCCCACTGA
  GGTGGCCAAAGTCCGCTTGCAGACGCAGACACAGGCGCAGAAGCAGCAGCGGCGGCTTTCGGCCTCGGGG
  CCGTTGGCTGTGCCCCCCATGTGTCCTGTGCCCCCAGCCTGCCCAGAGCCCAAGTACCGCGGGCCACTGC
  ACTGCCTGGCCACGGTAGCCCGTGAGGAGGGGCTGTGCGGCCTCTACAAGGGCAGCTCGGCCCTGGTCTT
  ACGGGACGGCCACTCCTTTGCCACCTACTTCCTTTCCTACGCGGTCCTCTGCGAGTGGCTCAGCCCCGCT
  GGCCACAGCCGGCCAGATGTCCCGGGCGTGCTGGTGGCCGGGGGCTGTGCAGGAGTCCTGGCCTGGGCTG
  TGGCCACCCCCATGGACGTGATCAAGTCGAGACTGCAGGCAGACGGGCAGGGCCAGAGGCGCTACCGGGG
  TCTCCTGCACTGTATGGTGACCAGCGTTCGAGAGGAGGGACCCCGGGTCCTTTTCAAGGGGCTGGTACTC
  AATTGCTGCCGCGCCTTCCCTGTCAACATGGTGGTCTTCGTCGCCTATGAGGCAGTGCTGAGGCTCGCCC
  GGGGTCTGCTCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221734 representing NM_207117
  Red=Cloning site Green=Tags(s)
  
  MDFVAGAIGGVCGVAVGYPLDTVKVRIQTEPKYTGIWHCVRDTYHRERVWGFYRGLSLPVCTVSLVSSVS
  FGTYRHCLAHICRLRYGNPDAKPTKADITLSGCASGLVRVFLTSPTEVAKVRLQTQTQAQKQQRRLSASG
  PLAVPPMCPVPPACPEPKYRGPLHCLATVAREEGLCGLYKGSSALVLRDGHSFATYFLSYAVLCEWLSPA
  GHSRPDVPGVLVAGGCAGVLAWAVATPMDVIKSRLQADGQGQRRYRGLLHCMVTSVREEGPRVLFKGLVL
  NCCRAFPVNMVVFVAYEAVLRLARGLLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207117
• ORF Size: 924 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207117.4
• RefSeq Size: 1733 bp
• RefSeq ORF: 927 bp
• Locus ID: 283600
• UniProt ID: Q6Q0C1
• Cytogenetics: 14q32.2
• Protein Families: Druggable Genome, Transmembrane
• MW: 33.3 kDa
• Gene Summary: This gene encodes a member of a large family of mitochondrial transporters. The nuclear-encoded carrier protein is embedded in the inner mitochondrial membrane. This member of the family is thought to be an uncoupling protein that uncouples mitochondrial respiration from ATP synthesis by dissipating the transmembrane proton gradient. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2017]