CLEC2D (NM_001004419) Human Tagged ORF Clone

CAT#: RC221680

CLEC2D (Myc-DDK-tagged)-Human C-type lectin domain family 2, member D (CLEC2D), transcript variant 2

Product Images

Circular map for RC221680

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  Western blot validation of overexpression lysate (Cat# LY423748) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221680 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CLEC2D protein (Cat# TP321680). The protein was produced from HEK293T cells transfected with CLEC2D cDNA clone (Cat# RC221680) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLEC2D
• Synonyms: CLAX; LLT1; OCIL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221680 representing NM_001004419
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGACAGTAACAATGTGGAGAAAGACATTACACCATCTGAATTGCCTGCAAACCCAGGTTGTCTGC
  ATTCAAAAGAGCATTCTATTAAAGCTACCTTAATTTGGCGCTTATTTTTCTTAATCATGTTTCTGACAAT
  CATAGTGTGTGGAATGGTTGCTGCTTTAAGCGCAATAAGAGCTAACTGCCATCAAGAGCCATCAGTATGT
  CTTCAAGCTGCATGCCCAGAAAGCTGGATTGGTTTTCAAAGAAAGTGTTTCTATTTTTCTGATGACACCA
  AGAACTGGACATCAAGTCAGAGGTTTTGTGACTCACAAGATGCTGATCTTGCTCAGGTTGAAAGCTTCCA
  GGAACTGAATTTCCTGTTGAGATATAAAGGCCCATCTGATCACTGGATTGGGCTGAGCAGAGAACAAGGC
  CAACCATGGAAATGGATAAATGGTACTGAATGGACAAGACAGTTAGTCATGAAAGAAGATGGTGCCAACT
  TGTATGTTGCAAAGGTTTCACAAGTTCCTCGAATGAATCCAAGACCTGTCATGGTTTCCTATCCTGGGAG
  CAGGAGAGTGTGCCTATTTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221680 representing NM_001004419
  Red=Cloning site Green=Tags(s)
  
  MHDSNNVEKDITPSELPANPGCLHSKEHSIKATLIWRLFFLIMFLTIIVCGMVAALSAIRANCHQEPSVC
  LQAACPESWIGFQRKCFYFSDDTKNWTSSQRFCDSQDADLAQVESFQELNFLLRYKGPSDHWIGLSREQG
  QPWKWINGTEWTRQLVMKEDGANLYVAKVSQVPRMNPRPVMVSYPGSRRVCLFE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004419
• ORF Size: 582 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004419.5
• RefSeq Size: 1821 bp
• RefSeq ORF: 585 bp
• Locus ID: 29121
• UniProt ID: Q9UHP7
• Cytogenetics: 12p13.31
• Protein Families: Druggable Genome, Transmembrane
• MW: 22.1 kDa
• Gene Summary: This gene encodes a member of the natural killer cell receptor C-type lectin family. The encoded protein inhibits osteoclast formation and contains a transmembrane domain near the N-terminus as well as the C-type lectin-like extracellular domain. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Oct 2010]