OC90 (NM_001080399) Human Tagged ORF Clone

CAT#: RC221622

OC90 (Myc-DDK-tagged)-Human otoconin 90 (OC90)

Product Images

Circular map for RC221622

• 

  Western blot validation of overexpression lysate (Cat# LY421617) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221622 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OC90
• Synonyms: PLA2L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221622 representing NM_001080399
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCTGAAGACGTTATCATCTGTGATACATTACAGCCATGTCACGTGGCCATGGCAACAGATATCA
  CTTTCTTCAGTGGGATGTTTAAAAATGTGGAAAGTGTGGCTGAAATTTTTGATTGCCTGGGCCCCCACTT
  CACCTGGCTGCAGGCTGTCTTCACCAATTTCCCTGTGCTGATCCAGTTTGTCAATGGTATGAAGTGTGTG
  GCTGGTCTCTGCCCCCGAGACTTTGAAGACTATGGTTGCACCTGCAGGTTTGAGATGGAAGGGTTGCCTG
  TGGATGAATCTGACAGCTGCTGCTTCCAGCACCGCAGGTGCTATGAGGAGGCCGCTGAGATGGACTGTCT
  CCAAGACCCCGCCAAACTTAGCACAGAGGTCAATTGTGTCAGCAAGAAGATCATATGTGAGTCCAAGGAC
  AACTGTGAGCACCTGCTGTGTACCTGTGATAAGGCTGCCATAGAGTGCTTGGCTCGATCCAGCCTCAACT
  CTTCCCTGAACCTTCTGGACACCTCCTTCTGCCTGGCTCAGACTCCAGAGACAACCATCAAGGAAGACTT
  GACAACACTTCTGCCCAGAGTGGTTCCTGTGGAGCCCACAGACACCAGCCTGACAGCCCTTTCAGGAGAA
  GTGGCTGCAGAGACTGAGGCTGACAGACTGATCACTCTCTCCAAGAAAAAAGCAGGCCACGATCAGGAAG
  GAGTGGGAGCTGCTAGGGCTACGTCCCCTCCAGGATCTGCAGAGATAGTTGCAACAAGGGTTACAGCTAA
  AATTGTAACCCTTGTCCCTGCTGGCATTAAATCTCTGGGGCTGGCAGTGTCATCTGTTGAAAATGATCCT
  GAGGAGACCACTGAAAAAGCCTGTGACAGATTCACCTTCCTGCACCTGGGAAGTGGGGACAACATGCAGG
  TGATGCCACAGCTTGGAGAGATGCTCTTTTGTCTGACATCCCGGTGCCCGGAGGAATTTGAGTCTTATGG
  CTGTTACTGTGGACAAGAAGGAAGAGGCGAGCCAAGGGATGACCTAGACAGGTGCTGCTTGTCCCATCAC
  TGCTGCCTAGAGCAAGTGAGAAGGCTGGGCTGCCTGCTTGAGAGGCTTCCTTGGTCACCGGTGGTGTGTG
  TGGATCATACGCCCAAGTGTGGGGGCCAAAGCCTGTGTGAGAAGTTGCTCTGTGCCTGTGACCAGACGGC
  AGCTGAGTGCATGACCTCTGCCTCCTTTAACCAAAGCCTCAAGTCCCCAAGCAGACTCGGGTGCCCTGGG
  CAGCCAGCAGCCTGTGAAGACAGCCTGCACCCTGTGCCCGCAGCCCCCACCCTGGGCTCCAGCTCTGAGG
  AGGACAGCGAGGAGGACCCTCCACAGGAGGACCTCGGCAGAGCCAAGAGGTTTCTGCGGAAGTCACTGGG
  TCCCTTGGGGATCGGGCCTCTTCATGGAAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221622 representing NM_001080399
  Red=Cloning site Green=Tags(s)
  
  MAAEDVIICDTLQPCHVAMATDITFFSGMFKNVESVAEIFDCLGPHFTWLQAVFTNFPVLIQFVNGMKCV
  AGLCPRDFEDYGCTCRFEMEGLPVDESDSCCFQHRRCYEEAAEMDCLQDPAKLSTEVNCVSKKIICESKD
  NCEHLLCTCDKAAIECLARSSLNSSLNLLDTSFCLAQTPETTIKEDLTTLLPRVVPVEPTDTSLTALSGE
  VAAETEADRLITLSKKKAGHDQEGVGAARATSPPGSAEIVATRVTAKIVTLVPAGIKSLGLAVSSVENDP
  EETTEKACDRFTFLHLGSGDNMQVMPQLGEMLFCLTSRCPEEFESYGCYCGQEGRGEPRDDLDRCCLSHH
  CCLEQVRRLGCLLERLPWSPVVCVDHTPKCGGQSLCEKLLCACDQTAAECMTSASFNQSLKSPSRLGCPG
  QPAACEDSLHPVPAAPTLGSSSEEDSEEDPPQEDLGRAKRFLRKSLGPLGIGPLHGR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080399
• ORF Size: 1431 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080399.1, NP_001073868.1
• RefSeq Size: 1434 bp
• RefSeq ORF: 1434 bp
• Locus ID: 729330
• UniProt ID: Q02509
• Cytogenetics: 8q24.22
• Protein Families: Secreted Protein
• MW: 51.5 kDa
• Gene Summary: Major protein of the otoconia, a calcium carbonate structure in the saccule and utricle of the ear. Together with OTOL1, acts as a scaffold for otoconia biomineralization: sequesters calcium and forms interconnecting fibrils between otoconia that are incorporated into the calcium crystal structure. Together with OTOL1, modulates calcite crystal morphology and growth kinetics. It is unlikely that this protein has phospholipase A2 activity.[UniProtKB/Swiss-Prot Function]