TAG1 (CNTN2) (NM_005076) Human Tagged ORF Clone

CAT#: RC221569

CNTN2 (Myc-DDK-tagged)-Human contactin 2 (axonal) (CNTN2)

Product Images

Circular map for RC221569

• 

  Western blot validation of overexpression lysate (Cat# LY417560) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221569 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAG1
• Synonyms: AXT; FAME5; TAG-1; TAX; TAX1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221569 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACAGCCACCAGGAGGAAGCCACACCTGCTGCTGGTAGCTGCTGTGGCCCTTGTCTCCTCTTCAG
  CTTGGAGTTCAGCCCTGGAATCCCAAACCACCTTCGGGCCTGTCTTTGAAGACCAGCCCCTCAGTGTGCT
  ATTCCCAGAGGAGTCCACGGAGGAGCAGGTGTTGCTGGCATGCCGCGCCCGGGCCAGCCCTCCAGCCACC
  TATCGGTGGAAGATGAATGGTACCGAGATGAAGCTGGAGCCAGGTTCCCGTCACCAGCTGGTGGGGGGCA
  ACCTGGTCATCATGAACCCCACCAAGGCACAGGATGCCGGGGTCTACCAGTGCCTGGCCTCCAACCCAGT
  GGGCACCGTTGTCAGCAGGGAGGCCATCCTCCGCTTCGGCTTTCTGCAGGAATTCTCCAAGGAGGAGCGA
  GACCCAGTGAAAGCTCATGAAGGCTGGGGGGTGATGTTGCCCTGTAACCCACCTGCCCACTACCCAGGCT
  TGTCCTACCGCTGGCTCCTCAACGAGTTCCCCAACTTCATCCCGACGGACGGGCGTCACTTCGTGTCCCA
  GACCACAGGGAACCTGTACATTGCCCGAACCAATGCCTCAGACCTGGGCAACTACTCCTGTTTGGCCACC
  AGCCACATGGACTTCTCCACCAAGAGCGTCTTCAGCAAGTTTGCTCAGCTCAACCTGGCTGCTGAAGATA
  CCCGGCTCTTTGCACCCAGCATCAAGGCCCGGTTCCCAGCAGAGACCTATGCACTGGTGGGGCAGCAGGT
  CACCCTGGAGTGCTTCGCCTTTGGGAACCCTGTCCCCCGGATCAAGTGGCGCAAAGTGGACGGCTCCCTG
  TCCCCGCAGTGGACCACAGCTGAGCCCACCCTGCAGATCCCCAGCGTCAGCTTTGAGGATGAGGGCACCT
  ACGAGTGTGAGGCGGAGAACTCCAAGGGCCGAGACACCGTGCAGGGCCGCATCATCGTGCAGGCTCAGCC
  TGAGTGGCTAAAAGTGATCTCGGACACAGAGGCTGACATTGGCTCCAACCTGCGTTGGGGCTGTGCAGCC
  GCCGGCAAGCCCCGGCCTACAGTGCGCTGGCTGCGGAACGGGGAGCCTCTGGCCTCCCAGAACCGGGTGG
  AGGTGTTGGCTGGGGACCTGCGGTTCTCCAAGCTGAGCCTGGAAGACTCGGGCATGTACCAGTGTGTGGC
  AGAGAATAAGCACGGTACCATCTACGCCAGCGCCGAGCTAGCCGTGCAAGCACTCGCCCCTGACTTCAGG
  CTGAATCCCGTGAGGCGTCTGATCCCCGCGGCCCGCGGGGGAGAGATCCTTATCCCCTGCCAGCCCCGGG
  CAGCTCCAAAGGCCGTGGTGCTCTGGAGCAAAGGCACGGAGATTTTGGTCAACAGCAGCAGAGTGACTGT
  AACTCCAGATGGCACCTTGATCATAAGAAACATCAGCCGGTCAGATGAAGGCAAATACACCTGCTTTGCT
  GAGAACTTCATGGGCAAAGCCAACAGCACTGGAATCCTATCTGTGCGAGATGCAACCAAAATCACTCTAG
  CCCCCTCAAGTGCCGACATCAACTTGGGTGACAACCTGACCCTACAGTGCCATGCCTCCCACGACCCCAC
  CATGGACCTCACCTTCACCTGGACCCTGGACGACTTCCCCATCGACTTTGATAAGCCTGGAGGGCACTAC
  CGGAGAACTAATGTGAAGGAGACCATTGGGGATCTGACCATCCTGAACGCCCAGCTGCGCCATGGGGGGA
  AGTACACGTGCATGGCCCAGACGGTGGTGGACAGCGCGTCCAAGGAGGCCACAGTCCTGGTCCGAGGTCC
  GCCAGGTCCCCCAGGAGGTGTGGTGGTGAGGGACATTGGCGACACCACCATCCAGCTCAGCTGGAGCCGT
  GGCTTCGACAACCACAGCCCCATCGCTAAGTACACCCTGCAAGCTCGCACTCCACCTGCAGGGAAGTGGA
  AGCAGGTTCGGACCAATCCTGCAAACATCGAGGGCAATGCCGAGACTGCACAGGTGCTGGGCCTCACCCC
  CTGGATGGACTATGAGTTCCGGGTCATAGCCAGCAACATTCTGGGCACTGGGGAGCCTAGTGGGCCCTCC
  AGCAAAATCCGGACCAGGGAAGCAGCCCCCTCGGTGGCACCCTCAGGACTCAGCGGAGGAGGTGGAGCCC
  CCGGAGAGCTCATCGTCAACTGGACGCCCATGTCACGGGAGTACCAGAACGGAGACGGCTTCGGCTACCT
  GCTGTCCTTCCGCAGGCAGGGCAGCACTCACTGGCAGACCGCCCGGGTGCCTGGCGCCGATGCCCAGTAC
  TTTGTCTACAGCAACGAGAGCGTCCGGCCCTACACGCCCTTTGAGGTCAAGATCCGCAGCTACAACCGCC
  GCGGGGATGGGCCCGAGAGCCTCACTGCACTCGTGTACTCAGCTGAGGAAGAGCCCAGGGTGGCCCCTAC
  CAAGGTGTGGGCCAAAGGGGTCTCATCCTCAGAGATGAACGTGACCTGGGAACCCGTGCAGCAGGACATG
  AATGGTATCCTCCTGGGGTATGAGATCCGCTACTGGAAAGCTGGGGACAAAGAAGCAGCTGCGGACCGAG
  TGAGGACAGCAGGGCTGGACACCAGTGCCCGAGTCAGCGGCCTGCATCCCAACACCAAGTACCATGTGAC
  CGTGAGGGCCTACAACCGGGCTGGCACTGGGCCTGCCAGCCCTTCTGCCAACGCCACGACCATGAAGCCC
  CCTCCGCGGCGACCTCCTGGCAACATCTCCTGGACTTTCTCAAGCTCTAGTCTTAGCATTAAGTGGGACC
  CTGTGGTCCCTTTCCGAAATGAGTCTGCAGTCACCGGCTATAAGATGCTGTACCAGAATGACTTACACCT
  GACTCCCACGCTCCACCTCACCGGCAAGAACTGGATAGAAATCCCAGTGCCTGAAGACATTGGCCATGCC
  CTGGTACAAATTCGGACCACAGGGCCCGGAGGGGATGGGATCCCTGCAGAAGTCCACATCGTGAGGAATG
  GAGGCACAAGCATGATGGTGGAGAACATGGCAGTCCGCCCAGCACCACACCCTGGCACCGTCATTTCCCA
  CTCCGTGGCGATGCTGATCCTCATAGGCTCCCTGGAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221569 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGTATRRKPHLLLVAAVALVSSSAWSSALESQTTFGPVFEDQPLSVLFPEESTEEQVLLACRARASPPAT
  YRWKMNGTEMKLEPGSRHQLVGGNLVIMNPTKAQDAGVYQCLASNPVGTVVSREAILRFGFLQEFSKEER
  DPVKAHEGWGVMLPCNPPAHYPGLSYRWLLNEFPNFIPTDGRHFVSQTTGNLYIARTNASDLGNYSCLAT
  SHMDFSTKSVFSKFAQLNLAAEDTRLFAPSIKARFPAETYALVGQQVTLECFAFGNPVPRIKWRKVDGSL
  SPQWTTAEPTLQIPSVSFEDEGTYECEAENSKGRDTVQGRIIVQAQPEWLKVISDTEADIGSNLRWGCAA
  AGKPRPTVRWLRNGEPLASQNRVEVLAGDLRFSKLSLEDSGMYQCVAENKHGTIYASAELAVQALAPDFR
  LNPVRRLIPAARGGEILIPCQPRAAPKAVVLWSKGTEILVNSSRVTVTPDGTLIIRNISRSDEGKYTCFA
  ENFMGKANSTGILSVRDATKITLAPSSADINLGDNLTLQCHASHDPTMDLTFTWTLDDFPIDFDKPGGHY
  RRTNVKETIGDLTILNAQLRHGGKYTCMAQTVVDSASKEATVLVRGPPGPPGGVVVRDIGDTTIQLSWSR
  GFDNHSPIAKYTLQARTPPAGKWKQVRTNPANIEGNAETAQVLGLTPWMDYEFRVIASNILGTGEPSGPS
  SKIRTREAAPSVAPSGLSGGGGAPGELIVNWTPMSREYQNGDGFGYLLSFRRQGSTHWQTARVPGADAQY
  FVYSNESVRPYTPFEVKIRSYNRRGDGPESLTALVYSAEEEPRVAPTKVWAKGVSSSEMNVTWEPVQQDM
  NGILLGYEIRYWKAGDKEAAADRVRTAGLDTSARVSGLHPNTKYHVTVRAYNRAGTGPASPSANATTMKP
  PPRRPPGNISWTFSSSSLSIKWDPVVPFRNESAVTGYKMLYQNDLHLTPTLHLTGKNWIEIPVPEDIGHA
  LVQIRTTGPGGDGIPAEVHIVRNGGTSMMVENMAVRPAPHPGTVISHSVAMLILIGSLEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005076
• ORF Size: 3120 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005076.5
• RefSeq Size: 7670 bp
• RefSeq ORF: 3123 bp
• Locus ID: 6900
• UniProt ID: Q02246
• Cytogenetics: 1q32.1
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 113.5 kDa
• Gene Summary: This gene encodes a member of the contactin family of proteins, part of the immunoglobulin superfamily of cell adhesion molecules. The encoded glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein plays a role in the proliferation, migration, and axon guidance of neurons of the developing cerebellum. A mutation in this gene may be associated with adult myoclonic epilepsy. [provided by RefSeq, Sep 2016]