CLOCK (NM_004898) Human Tagged ORF Clone

CAT#: RC221408

CLOCK (Myc-DDK-tagged)-Human clock homolog (mouse) (CLOCK)

Product Images

Circular map for RC221408

• 

  Western blot validation of overexpression lysate (Cat# LY417668) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221408 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CLOCK
• Synonyms: bHLHe8; KAT13D
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221408 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTTTACCGTAAGCTGTAGTAAAATGAGCTCGATTGTTGACAGAGATGACAGTAGTATTTTTGATG
  GGTTGGTGGAAGAAGATGACAAGGACAAAGCGAAAAGAGTATCTAGAAACAAATCTGAAAAGAAACGTAG
  AGATCAATTTAATGTTCTCATTAAAGAACTGGGATCCATGCTTCCTGGTAATGCTAGAAAGATGGACAAA
  TCTACTGTTCTGCAGAAAAGCATTGATTTTTTACGAAAACATAAAGAAATCACTGCACAGTCAGATGCTA
  GTGAAATTCGACAGGACTGGAAACCTACATTCCTTAGTAATGAAGAGTTTACACAATTAATGTTAGAGGC
  TCTTGATGGTTTTTTTTTAGCAATCATGACAGATGGAAGCATAATATATGTGTCTGAGAGTGTAACTTCA
  TTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTTATCCCAGAAGGGGAACATT
  CAGAGGTTTATAAAATACTCTCTACTCATCTGCTGGAAAGTGATTCATTAACCCCAGAATATTTAAAATC
  AAAAAATCAGTTAGAATTCTGTTGTCACATGCTGCGAGGAACAATAGACCCAAAGGAGCCATCTACCTAT
  GAATATGTAAAATTTATAGGAAATTTCAAATCTTTAAACAGTGTATCCTCTTCAGCACACAATGGTTTTG
  AAGGAACTATACAACGCACACATAGGCCATCTTATGAAGATAGAGTTTGTTTTGTAGCTACTGTCAGGTT
  AGCTACACCTCAGTTCATCAAGGAAATGTGCACTGTTGAAGAACCCAATGAAGAGTTTACATCTAGACAT
  AGTTTAGAATGGAAGTTTCTGTTTCTAGATCACAGGGCACCACCCATAATAGGGTATTTGCCATTTGAAG
  TTCTGGGAACATCAGGCTATGATTACTATCATGTGGATGACCTAGAAAATTTGGCAAAATGTCATGAGCA
  CTTAATGCAATATGGGAAAGGCAAATCATGTTATTATAGGTTCCTGACTAAGGGGCAACAGTGGATTTGG
  CTTCAGACTCATTATTATATCACTTACCATCAGTGGAATTCAAGGCCAGAGTTTATTGTTTGTACTCACA
  CTGTAGTAAGTTATGCAGAAGTTAGGGCTGAAAGACGACGAGAACTTGGCATTGAAGAGTCTCTTCCTGA
  GACAGCTGCTGACAAAAGCCAAGATTCTGGGTCAGATAATCGTATAAACACAGTCAGTCTCAAGGAAGCA
  TTGGAAAGGTTTGATCACAGCCCAACCCCTTCTGCCTCTTCTCGGAGTTCAAGAAAATCATCTCACACGG
  CCGTCTCAGACCCTTCCTCAACACCAACCAAGATCCCGACGGATACGAGCACTCCACCCAGGCAGCATTT
  ACCAGCTCATGAGAAGATGGTGCAAAGAAGGTCATCATTTAGTAGTCAGTCCATAAATTCCCAGTCTGTT
  GGTTCATCATTAACACAGCCAGTGATGTCTCAAGCTACAAATTTACCAATTCCACAAGGCATGTCCCAGT
  TTCAGTTTTCAGCTCAATTAGGAGCCATGCAACATCTGAAAGACCAATTGGAACAACGGACACGCATGAT
  AGAAGCAAATATTCATCGGCAACAAGAAGAACTAAGAAAAATTCAAGAACAACTTCAGATGGTCCATGGT
  CAGGGGCTGCAGATGTTTTTGCAACAATCAAATCCTGGGTTGAATTTTGGTTCCGTTCAACTTTCTTCTG
  GAAATTCATCTAACATCCAGCAACTTGCACCTATAAATATGCAAGGCCAAGTTGTTCCTACTAACCAGAT
  TCAAAGTGGAATGAATACTGGACACATTGGCACAACTCAGCACATGATACAACAACAGACTTTACAGAGT
  ACATCAACTCAGAGTCAACAAAATGTACTGAGTGGGCACAGTCAGCAAACATCTCTACCCAGTCAGACAC
  AGAGCACTCTTACAGCCCCACTGTATAACACTATGGTGATTTCTCAGCCTGCAGCCGGAAGCATGGTCCA
  GATTCCATCTAGTATGCCACAAAACAGCACCCAGAGTGCTGCAGTAACTACATTCACTCAGGACAGGCAG
  ATAAGATTTTCTCAAGGTCAACAACTTGTGACCAAATTAGTGACTGCTCCTGTAGCTTGTGGGGCAGTCA
  TGGTACCTAGTACTATGCTTATGGGCCAGGTGGTGACTGCATATCCTACTTTTGCTACACAACAGCAACA
  GTCACAGACATTGTCAGTAACGCAGCAGCAGCAGCAGCAGAGCTCCCAGGAGCAGCAGCTCACTTCAGTT
  CAGCAACCATCTCAGGCTCAGCTGACCCAGCCACCGCAACAATTTTTACAGACTTCTAGGTTGCTCCATG
  GGAATCCCTCAACTCAACTCATTCTCTCTGCTGCATTTCCTCTACAACAGAGCACCTTCCCTCAGTCACA
  TCACCAGCAACATCAGTCTCAGCAACAGCAGCAACTCAGCCGGCACAGGACTGACAGCTTGCCCGACCCT
  TCCAAGGTTCAACCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221408 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MLFTVSCSKMSSIVDRDDSSIFDGLVEEDDKDKAKRVSRNKSEKKRRDQFNVLIKELGSMLPGNARKMDK
  STVLQKSIDFLRKHKEITAQSDASEIRQDWKPTFLSNEEFTQLMLEALDGFFLAIMTDGSIIYVSESVTS
  LLEHLPSDLVDQSIFNFIPEGEHSEVYKILSTHLLESDSLTPEYLKSKNQLEFCCHMLRGTIDPKEPSTY
  EYVKFIGNFKSLNSVSSSAHNGFEGTIQRTHRPSYEDRVCFVATVRLATPQFIKEMCTVEEPNEEFTSRH
  SLEWKFLFLDHRAPPIIGYLPFEVLGTSGYDYYHVDDLENLAKCHEHLMQYGKGKSCYYRFLTKGQQWIW
  LQTHYYITYHQWNSRPEFIVCTHTVVSYAEVRAERRRELGIEESLPETAADKSQDSGSDNRINTVSLKEA
  LERFDHSPTPSASSRSSRKSSHTAVSDPSSTPTKIPTDTSTPPRQHLPAHEKMVQRRSSFSSQSINSQSV
  GSSLTQPVMSQATNLPIPQGMSQFQFSAQLGAMQHLKDQLEQRTRMIEANIHRQQEELRKIQEQLQMVHG
  QGLQMFLQQSNPGLNFGSVQLSSGNSSNIQQLAPINMQGQVVPTNQIQSGMNTGHIGTTQHMIQQQTLQS
  TSTQSQQNVLSGHSQQTSLPSQTQSTLTAPLYNTMVISQPAAGSMVQIPSSMPQNSTQSAAVTTFTQDRQ
  IRFSQGQQLVTKLVTAPVACGAVMVPSTMLMGQVVTAYPTFATQQQQSQTLSVTQQQQQQSSQEQQLTSV
  QQPSQAQLTQPPQQFLQTSRLLHGNPSTQLILSAAFPLQQSTFPQSHHQQHQSQQQQQLSRHRTDSLPDP
  SKVQPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004898
• ORF Size: 2538 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004898.2, NP_004889.1
• RefSeq Size: 10472 bp
• RefSeq ORF: 2541 bp
• Locus ID: 9575
• UniProt ID: O15516
• Cytogenetics: 4q12
• Domains: PAS, HLH, PAC
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Circadian rhythm - mammal
• MW: 95.3 kDa
• Gene Summary: The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]