DCST1 (NM_152494) Human Tagged ORF Clone

CAT#: RC221368

DCST1 (Myc-DDK-tagged)-Human DC-STAMP domain containing 1 (DCST1), transcript variant 1

Product Images

Circular map for RC221368

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DCST1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221368 representing NM_152494
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATTAAACATCATCAGAATGGCACAAGAGGGCAAAGAAGAAAACAGCCTCATACCACGGTGCAGA
  GGCTCCTGACCTGGGGGCTGCCAGTCTCCTGTAGCTGGTTCCTGTGGCGCCAGCCGGGCGAGTTTCCTGT
  CACTGCTCTTCTGCTGGGGGCAGGCGCTGGGGGGCTCCTGGCCATAGGTCTCTTTCAGCTCCTGGTGAAC
  CCCATGAACATCTACGAAGAACAGAAGATTATGTTCTTGTACAGCTTGGTGGGCTTGGGGGCCATGGGCT
  GGGGGACCTCCCCTCACATCCGCTGTGCCAGCCTCCTACTAGTACCCAAGATGCTGGGCAAGGAAGGCAG
  GCTCTTTGTCCTGGGATACGCCTTGGCTGCCATCTATGTGGGGCCAGTAGCCAATCTGCGACACAATCTC
  AACAACGTGATCGCATCGCTGGGCTGCACCGTGGAGCTGCAGATCAACAACACCCGCGCAGCTTGGCGCA
  TCTCCACGGCCCCCTTACGGGCCATGTTCAAGGACCTGCTGAGTAGCAAAGAATTGCTGAGAGCAGAGAC
  TCGGAACATCTCCGCCACTTTTGAGGACCTGGATGCCCAGGTGAATAGTGAGACGGGCTACACGCCTGAG
  GATACCATGGACTCAGGGGAGACAGCCCAGGGCAGGGAGGCCCGCCAAGCCCCAGCCTCCAGACTCCACC
  TGTCGACACAGAAGATGTATGAGCTGAAGACCAAGCTGCGTTGCTCCTATGTGGTGAACCAGGCCATACT
  CAGCTGCCGTCGTTGGTTTGACCGCAAGCATGAACAGTGCATGAAGCACATCTGGGTCCCACTCCTCACC
  CACCTGCTCTGCCTGCCTATGAAGTTCAAGTTCTTCTGTGGCATTGCCAAGGTGATGGAGGTTTGGTGCC
  GCAATCGCATCCCAGTGGAAGGCAACTTTGGGCAGACCTACGACTCCCTCAACCAGTCTATTCGTGGCCT
  GGATGGGGAATTTTCAGCCAATATTGACTTCAAGGAAGAGAAGCAGGCTGGGGTGCTGGGGCTCAACACA
  AGCTGGGAGCGCGTGAGCACCGAGGTGCGGGACTACGTGTACCGCCAGGAGGCCCGGCTGGAGTGGGCCC
  TGGGGCTGCTGCACGTGCTGCTCTCCTGCACTTTCCTGCTGGTCCTGCATGCGTCTTTCTCCTACATGGA
  CAGCTATAACCATGACATTCGTTTTGACAACATCTACATCAGTACCTACTTCTGCCAGATCGATGACCGC
  AGGAAGAAGCTGGGCAAACGGACTCTGCTGCCACTCCGCAAAGCTGAGGAGAAAACCGTCATCTTCCCTT
  GCAAGCCCACCATCCAGGCCTCAGAAATGAGCAATGTGGTGAGGGAGCTCCTGGAGACACTGCCCATTCC
  GCTGCTGCTGGTGGTGCTGTGTGGCTTGGACTGGGCTCTCTACTCCATCTTCGACACCATCCGCCACCAC
  TCCTTCCTGCAGTACTCCTTCCGCAGCAGTCATAAACTGGAGGTGAAGGTCGGGGGAGACTCCCTGCTAG
  CCCGGCTTCTTCGAAAAACCATTGGGGCCCTGAACACCTCCTCAGAGACAGTGATGGAATCAAACAACAT
  GCCCTGCCTGCCCCAGCCTGTGGGCCTGGATGCCAGGGCCTACTGGAGAGCTGCAGTACCGATTGGCCTG
  TTAGTGTGTCTCTGCCTGTTACAGGCTTTTGGCTACCGACTCCGGAGGGTCATCGCAGCCTTCTACTTCC
  CCAAGCGAGAGAAGAAGCGGATCCTGTTCCTCTACAATGACCTATTGAAGAAAAGAGCAGCCTTCACCAA
  ACTCAGGAGGGCCGCTATCCTGAGGCGGGAGCGACAGCAGAAGGCTCCGCGCCACCCGCTGGCGGATATC
  CTGCACCGCGGCTGCCCGCTCCTGCGCCGCTGGCTGTGCCGGCGCTGCGTGGTGTGCCAGGCACCCGAGA
  CGCCCGAGTCCTACGTGTGCCGGACGCTGGACTGCGAGGCCGTGTACTGCTGGTCGTGCTGGGACGACAT
  GCGGCAGCGGTGCCCGGTCTGCACGCCCCGCGAAGAGCTCTCTTCCTCCGCCTTTAGTGACAGCAACGAC
  GACACTGCCTACGCGGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221368 representing NM_152494
  Red=Cloning site Green=Tags(s)
  
  MDIKHHQNGTRGQRRKQPHTTVQRLLTWGLPVSCSWFLWRQPGEFPVTALLLGAGAGGLLAIGLFQLLVN
  PMNIYEEQKIMFLYSLVGLGAMGWGTSPHIRCASLLLVPKMLGKEGRLFVLGYALAAIYVGPVANLRHNL
  NNVIASLGCTVELQINNTRAAWRISTAPLRAMFKDLLSSKELLRAETRNISATFEDLDAQVNSETGYTPE
  DTMDSGETAQGREARQAPASRLHLSTQKMYELKTKLRCSYVVNQAILSCRRWFDRKHEQCMKHIWVPLLT
  HLLCLPMKFKFFCGIAKVMEVWCRNRIPVEGNFGQTYDSLNQSIRGLDGEFSANIDFKEEKQAGVLGLNT
  SWERVSTEVRDYVYRQEARLEWALGLLHVLLSCTFLLVLHASFSYMDSYNHDIRFDNIYISTYFCQIDDR
  RKKLGKRTLLPLRKAEEKTVIFPCKPTIQASEMSNVVRELLETLPIPLLLVVLCGLDWALYSIFDTIRHH
  SFLQYSFRSSHKLEVKVGGDSLLARLLRKTIGALNTSSETVMESNNMPCLPQPVGLDARAYWRAAVPIGL
  LVCLCLLQAFGYRLRRVIAAFYFPKREKKRILFLYNDLLKKRAAFTKLRRAAILRRERQQKAPRHPLADI
  LHRGCPLLRRWLCRRCVVCQAPETPESYVCRTLDCEAVYCWSCWDDMRQRCPVCTPREELSSSAFSDSND
  DTAYAG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152494
• ORF Size: 2118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152494.1, NP_689707.1
• RefSeq Size: 2314 bp
• RefSeq ORF: 2121 bp
• Locus ID: 149095
• UniProt ID: Q5T197
• Cytogenetics: 1q21.3
• Protein Families: Druggable Genome, Transmembrane
• MW: 80.5 kDa
• Gene Summary: This gene encodes a protein with a domain similar to one found in dendritic cells (PMID:11169400) which play a key role in antigen processing and display for immune responses. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]